MD05G1234100.v1.1

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0028824
Physical Location & Seq
Reverse (-)
36645757 .. 36647191
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1234100.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGACCACTGCAAGTTTTGATCATGCTCGTAGTACCGATCGTCGGAGTCGCAACCGAATAAGAAAGAGAAAAACTAGTAGGATGGATAGATTCAGTAATCTCCCGATCGAGATCGCTCATGAAATTATGTCTCGCCTCAACTTCAAAGACCTTACTAGGGTTGGCTGCGTGTCCAAAGCATGCAGAGAGTTTTATATATCGACCCCGTACTTGAATTTTCATGACTTTTCTGGTGTGGATATATCCACTCGTCGAAAGAACAAAAAGGTTTTCAATTCTTTGAAAAGGTTCTTCATTCAACGTGGGGTGCATCTTAAGATACAATGCTTTCGTTTTCGTTGGTGCTTTTTTGATGACGACCATGATGAGGATTTCCGATTGCGATTGAGGGAATGGATTGAAATTGCTGTGAGGTGCAATGTTGAAGTGCTTCATCTTGTTGTCACTTATATTAGGTTTGGAGAGCTTCCTGCTTCCATCTTACACTGTAAATCGTTGAAATCTCTGCTTGTGGACATGAAGTGCACGATTAGTGATGTGCCCTTCATCGATTTTTTGTCTAATCTCGATTACCTGAGGTTGAAAGATGTTTCGATAAAGGATGAAGATGATCGATTTTTGAAGTGGATTTCATGTTGCTGCACTTCCATGAAGGAATTACTTCTTAAAGATTGTGGATTTTGGCATGAGTCTGATTTTTACCACATCCCCGAACGCCATGTTAACATCTCAGGTGACAGACTGAAAAAATTACATTTAAATCCACAGCTTCGGAGGAAGACAAAAAAATGGGTCAACATTTATGCTCCAAACCTCAAGTGTTTGGAATGGTTTGGGGAATGTACGACTAGCCAAAATCTTGGAAACTTAATGTGCTTGGAGAAGGCTGAGATTTCTTCGTCAATTCCGATCTGTAACCATGATATCTTAATTGATTTTCTTTGTAGCATCCGCAAGGTTAAAGTTCTTATTCTGCATCCAGATATTCTTGAGCTTCTATGGTTAAATATGGACGATCGGAAATGGCAAGATCTTTCATTTATTTCTCAAGCCAAGGAGGTAGTCATACCATATGATGATTATGAGCCTGATATTGAGCCCGCATATTCTATTCTCGAACATGCTCACGATTTAGAGAAGATGACCATATACTATCCTCACTGTTTCGATGCACATGATGACCTGACGGATGTATATGATGACCTGATGGAGTTGAGAACAGAAGTGATGTCGAAAGGAATTCACTTCAACATAATTACGACCTCGAGGGGTAGCTCCCTACTTAGTTCGATTCAATCTTTGGCTGATTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

439

Amino Acids

51.76

Weight (kDa)

7.24

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 31 - 63 2.4e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 126 - 326 4.9e-09 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028824)

Species Orthologous Gene IDs
malus_domestica MD05G1234100.v1.1
pyrus_communis pycom05g21150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1264
AciI CCGC 2 cut(s) 952, 1101
AcsI RAATTY 2 cut(s) 214, 1239
AfaI GTAC 3 cut(s) 34, 209, 844
AfiI CCNNNNNNNGG 3 cut(s) 42, 157, 367
AflII CTTAAG 1 cut(s) 314
AhlI ACTAGT 1 cut(s) 74
AluBI AGCT 4 cut(s) 466, 769, 994, 1275
AluI AGCT 4 cut(s) 466, 769, 994, 1275
Alw21I GWGCWC 1 cut(s) 527
Alw26I GTCTC 1 cut(s) 135
Alw44I GTGCAC 1 cut(s) 523
Ama87I CYCGRG 1 cut(s) 1264
ApaLI GTGCAC 1 cut(s) 523
ApeKI GCWGC 2 cut(s) 165, 639
ApoI RAATTY 2 cut(s) 214, 1239
Asp700I GAANNNNTTC 3 cut(s) 287, 429, 660
AsuHPI GGTGA 1 cut(s) 746
AvaI CYCGRG 1 cut(s) 1264
AxyI CCTNAGG 1 cut(s) 575
BaeGI GKGCMC 2 cut(s) 527, 543
BanII GRGCYC 1 cut(s) 1101
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 2 cut(s) 152, 626
BccI CCATC 3 cut(s) 76, 485, 1201
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 135
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 3 cut(s) 75, 156, 849
BfrI CTTAAG 1 cut(s) 314
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 2 cut(s) 166, 640
BlsI GCNGC 2 cut(s) 167, 641
BmeT110I CYCGRG 1 cut(s) 1264
BmsI GCATC 4 cut(s) 319, 957, 985, 1159
BpiI GAAGAC 1 cut(s) 785
BpuEI CTTGAG 3 cut(s) 800, 1010, 1032
Bsa29I ATCGAT 2 cut(s) 549, 613
BsaBI GATNNNNATC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 1053
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 157, 367
Bse21I CCTNAGG 1 cut(s) 575
Bse3DI GCAATG 1 cut(s) 424
Bse8I GATNNNNATC 1 cut(s) 110
BseCI ATCGAT 2 cut(s) 549, 613
BseDI CCNNGG 1 cut(s) 1053
BseGI GGATG 6 cut(s) 87, 607, 705, 948, 976, 1195
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 3 cut(s) 42, 157, 367
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 3 cut(s) 566, 744, 879
BseSI GKGCMC 2 cut(s) 527, 543
BseXI GCAGC 2 cut(s) 152, 626
BsgI GTGCAG 1 cut(s) 625
Bsh1285I CGRYCG 3 cut(s) 40, 108, 1018
BshVI ATCGAT 2 cut(s) 549, 613
BsiEI CGRYCG 3 cut(s) 40, 108, 1018
BsiHKAI GWGCWC 1 cut(s) 527
BsiHKCI CYCGRG 1 cut(s) 1264
BslI CCNNNNNNNGG 3 cut(s) 42, 157, 367
BsmAI GTCTC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 1264
Bsp1286I GDGCHC 3 cut(s) 527, 543, 1101
Bsp143I GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
BspACI CCGC 2 cut(s) 952, 1101
BspCNI CTCAG 3 cut(s) 567, 743, 880
BspDI ATCGAT 2 cut(s) 549, 613
BspHI TCATGA 2 cut(s) 118, 220
BspTI CTTAAG 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 1053
BssMI GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
BssT1I CCWWGG 1 cut(s) 1053
Bst4CI ACNGT 2 cut(s) 488, 1163
BstAFI CTTAAG 1 cut(s) 314
BstC8I GCNNGC 2 cut(s) 181, 1101
BstDEI CTNAG 4 cut(s) 575, 730, 888, 1283
BstF5I GGATG 6 cut(s) 87, 607, 705, 948, 976, 1195
BstKTI GATC 8 cut(s) 22, 40, 108, 114, 613, 912, 1018, 1033
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
BstMCI CGRYCG 3 cut(s) 40, 108, 1018
BstNSI RCATGY 2 cut(s) 183, 1124
BstSLI GKGCMC 2 cut(s) 527, 543
BstV1I GCAGC 2 cut(s) 152, 626
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 1030
BstXI CCANNNNNNTGG 1 cut(s) 860
BstYI RGATCY 1 cut(s) 1030
Bsu15I ATCGAT 2 cut(s) 549, 613
Bsu36I CCTNAGG 1 cut(s) 575
BsuTUI ATCGAT 2 cut(s) 549, 613
BtsCI GGATG 6 cut(s) 87, 607, 705, 948, 976, 1195
BtsI GCAGTG 1 cut(s) 6
BtsIMutI CAGTG 3 cut(s) 6, 484, 1159
Cac8I GCNNGC 2 cut(s) 181, 1101
CciI TCATGA 2 cut(s) 118, 220
ClaI ATCGAT 2 cut(s) 549, 613
Csp6I GTAC 3 cut(s) 33, 208, 843
CviQI GTAC 3 cut(s) 33, 208, 843
DdeI CTNAG 4 cut(s) 575, 730, 888, 1283
DpnI GATC 8 cut(s) 21, 39, 107, 113, 612, 911, 1017, 1032
DpnII GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
DraI TTTAAA 1 cut(s) 759
Eco130I CCWWGG 1 cut(s) 1053
Eco24I GRGCYC 1 cut(s) 1101
Eco32I GATATC 1 cut(s) 925
Eco81I CCTNAGG 1 cut(s) 575
Eco88I CYCGRG 1 cut(s) 1264
EcoRI GAATTC 1 cut(s) 1239
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 1 cut(s) 1053
EcoT38I GRGCYC 1 cut(s) 1101
ErhI CCWWGG 1 cut(s) 1053
FauI CCCGC 1 cut(s) 1108
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 166, 640
FokI GGATG 6 cut(s) 94, 614, 692, 935, 963, 1202
FriOI GRGCYC 1 cut(s) 1101
Fsp4HI GCNGC 2 cut(s) 166, 640
FspBI CTAG 3 cut(s) 75, 156, 849
GluI GCNGC 2 cut(s) 166, 640
HincII GTYRAC 2 cut(s) 724, 796
HindII GTYRAC 2 cut(s) 724, 796
HinfI GANTC 4 cut(s) 46, 90, 689, 1291
HpaI GTTAAC 1 cut(s) 724
HphI GGTGA 1 cut(s) 746
Hpy166II GTNNAC 4 cut(s) 514, 525, 724, 796
Hpy188I TCNGA 6 cut(s) 45, 377, 694, 774, 909, 1020
Hpy188III TCNNGA 9 cut(s) 103, 109, 119, 221, 566, 980, 989, 1115, 1127
Hpy8I GTNNAC 4 cut(s) 514, 525, 724, 796
Hpy99I CGWCG 2 cut(s) 45, 255
HpyAV CCTTC 3 cut(s) 553, 646, 877
HpyCH4III ACNGT 2 cut(s) 488, 1163
HpyCH4IV ACGT 1 cut(s) 301
HpyCH4V TGCA 8 cut(s) 11, 183, 310, 417, 525, 642, 976, 1172
HpyF3I CTNAG 4 cut(s) 575, 730, 888, 1283
HpySE526I ACGT 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 19
KspAI GTTAAC 1 cut(s) 724
Kzo9I GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
LmnI GCTCC 2 cut(s) 811, 1280
LpnPI CCDG 8 cut(s) 216, 483, 587, 717, 993, 1101, 1196, 1217
Lsp1109I GCAGC 2 cut(s) 152, 626
LweI GCATC 4 cut(s) 319, 957, 985, 1159
MaeI CTAG 3 cut(s) 75, 156, 849
MaeII ACGT 1 cut(s) 301
MaeIII GTNAC 3 cut(s) 442, 734, 914
MalI GATC 8 cut(s) 21, 39, 107, 113, 612, 911, 1017, 1032
MboI GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
MboII GAAGA 5 cut(s) 283, 617, 790, 888, 1150
MflI RGATCY 1 cut(s) 1030
MhlI GDGCHC 3 cut(s) 527, 543, 1101
MlyI GAGTC 2 cut(s) 55, 698
MmeI TCCRAC 1 cut(s) 23
MroXI GAANNNNTTC 3 cut(s) 287, 429, 660
MseI TTAA 9 cut(s) 315, 666, 723, 758, 869, 929, 960, 1004, 1315
MslI CAYNNNNRTG 1 cut(s) 647
MspCI CTTAAG 1 cut(s) 314
NdeI CATATG 1 cut(s) 1072
NdeII GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
NmuCI GTSAC 2 cut(s) 442, 734
NspI RCATGY 2 cut(s) 183, 1124
PaeI GCATGC 1 cut(s) 183
PaeR7I CTCGAG 1 cut(s) 1264
PagI TCATGA 2 cut(s) 118, 220
PcsI WCGNNNNNNNCGW 1 cut(s) 905
PdmI GAANNNNTTC 3 cut(s) 287, 429, 660
PfeI GAWTC 2 cut(s) 90, 1291
PkrI GCNGC 2 cut(s) 167, 641
Ple19I CGATCG 3 cut(s) 40, 108, 1018
PleI GAGTC 2 cut(s) 54, 697
PpsI GAGTC 2 cut(s) 54, 697
PspXI VCTCGAGB 1 cut(s) 1264
PsuI RGATCY 1 cut(s) 1030
PvuI CGATCG 3 cut(s) 40, 108, 1018
RsaI GTAC 3 cut(s) 34, 209, 844
RsaNI GTAC 3 cut(s) 33, 208, 843
RseI CAYNNNNRTG 1 cut(s) 647
SaqAI TTAA 9 cut(s) 315, 666, 723, 758, 869, 929, 960, 1004, 1315
SatI GCNGC 2 cut(s) 166, 640
Sau3AI GATC 8 cut(s) 19, 37, 105, 111, 610, 909, 1015, 1030
SchI GAGTC 2 cut(s) 55, 698
SduI GDGCHC 3 cut(s) 527, 543, 1101
SfaNI GCATC 4 cut(s) 319, 957, 985, 1159
Sfr274I CTCGAG 1 cut(s) 1264
SlaI CTCGAG 1 cut(s) 1264
SmiI ATTTAAAT 1 cut(s) 759
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 5 cut(s) 314, 815, 989, 1047, 1264
SmoI CTYRAG 5 cut(s) 314, 815, 989, 1047, 1264
SpeI ACTAGT 1 cut(s) 74
SphI GCATGC 1 cut(s) 183
SsiI CCGC 2 cut(s) 952, 1101
SspMI CTAG 3 cut(s) 75, 156, 849
StyI CCWWGG 1 cut(s) 1053
SwaI ATTTAAAT 1 cut(s) 759
TaaI ACNGT 2 cut(s) 488, 1163
TaiI ACGT 1 cut(s) 304
TfiI GAWTC 2 cut(s) 90, 1291
Tru1I TTAA 9 cut(s) 315, 666, 723, 758, 869, 929, 960, 1004, 1315
Tru9I TTAA 9 cut(s) 315, 666, 723, 758, 869, 929, 960, 1004, 1315
TscAI CASTG 3 cut(s) 13, 491, 1166
TseFI GTSAC 2 cut(s) 442, 734
TseI GCWGC 2 cut(s) 165, 639
Tsp45I GTSAC 2 cut(s) 442, 734
TspGWI ACGGA 1 cut(s) 1202
TspRI CASTG 3 cut(s) 13, 491, 1166
Vha464I CTTAAG 1 cut(s) 314
VneI GTGCAC 1 cut(s) 523
XapI RAATTY 2 cut(s) 214, 1239
XceI RCATGY 2 cut(s) 183, 1124
XhoI CTCGAG 1 cut(s) 1264
XmnI GAANNNNTTC 3 cut(s) 287, 429, 660
XspI CTAG 3 cut(s) 75, 156, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.