MD05G1324900.v1.1

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024454
Physical Location & Seq
Reverse (-)
45138567 .. 45139734
1168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1324900.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGACCGACCCGGACCCGAGTCTGCTCCCCCGCAACCCCTACTTTCCGACCGGGTCCTCATAAGCGGCGTCGTCACACCCGTGGCTCTATTCTCCGACGGAGTGCTCCAGTGGTCGGAGAGAAGTCAACGGAGCTTGATCGTCGAGAAGGAGGTCCTGGGGTTCGCGGCGGAAGGTCCGAAGATTAGGATAAGAGCTTTTGTGGAAGGCGGAGCTGGAATCTGCTGTGGCGCGAACAGAGGAGCTGTGGCGAGGAAGGACTTGGTGTTCGAGCCTCTCTCTGAGGACTCGCACAGGCTCTGGTGCCAGAAGCTCGGGGATTACATCGATTCTCTCGGACGGCCAAAGAGGCTGTTTGTTTTTGTGAATCCGTTTGGTGGTAGCAAATCTGCTTCAAAGATTTATGTTGATCACGTAAAACCTCTTTTCGAGGATGCCGATATCCAATTTACGCTGCAAGAAACTCAATATCAACTACATGCGAAGGAAGTTGTGAAAACACTAGATCTTTCAAAGTATGATGGCATTGTTTGTGTTAGTGGGGACGGAATCTTGGTTGAGGTCGGTTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

192

Amino Acids

20.95

Weight (kDa)

5.66

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 117 - 188 4.9e-16 Diacylglycerol kinase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024454)

Species Orthologous Gene IDs
malus_domestica MD05G1324900.v1.1
pyrus_communis pycom10g25530
rosa_multiflora Rmu_co8106934.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 303
AccII CGCG 2 cut(s) 167, 233
AciI CCGC 5 cut(s) 32, 66, 167, 170, 210
AcoI YGGCCR 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 69
AfiI CCNNNNNNNGG 2 cut(s) 115, 377
AgsI TTSAA 2 cut(s) 396, 513
AjnI CCWGG 1 cut(s) 156
AleI CACNNNNGTG 1 cut(s) 80
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 5 cut(s) 135, 197, 215, 245, 313
AluI AGCT 5 cut(s) 135, 197, 215, 245, 313
Alw21I GWGCWC 1 cut(s) 108
AlwNI CAGNNNCTG 1 cut(s) 300
Ama87I CYCGRG 2 cut(s) 17, 314
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 1 cut(s) 454
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 5 cut(s) 4, 14, 55, 154, 176
AsuC2I CCSGG 2 cut(s) 12, 53
AvaI CYCGRG 2 cut(s) 17, 314
AvaII GGWCC 5 cut(s) 4, 14, 55, 154, 176
BanI GGYRCC 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 441
BccI CCATC 1 cut(s) 515
BceAI ACGGC 1 cut(s) 356
BciT130I CCWGG 1 cut(s) 158
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 2 cut(s) 12, 53
BfaI CTAG 1 cut(s) 503
BglI GCCNNNNNGGC 1 cut(s) 349
BglII AGATCT 1 cut(s) 505
BisI GCNGC 3 cut(s) 67, 168, 455
BlsI GCNGC 3 cut(s) 68, 169, 456
Bme1390I CCNGG 3 cut(s) 12, 53, 158
Bme18I GGWCC 5 cut(s) 4, 14, 55, 154, 176
BmeT110I CYCGRG 2 cut(s) 17, 314
BmgT120I GGNCC 5 cut(s) 4, 14, 55, 154, 176
BmiI GGNNCC 3 cut(s) 16, 56, 305
BmrFI CCNGG 3 cut(s) 12, 53, 158
BmsI GCATC 1 cut(s) 424
BoxI GACNNNNGTC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 1 cut(s) 92
BpuMI CCSGG 2 cut(s) 12, 53
Bsa29I ATCGAT 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 415
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 81, 157
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 377
Bse1I ACTGG 1 cut(s) 109
BseBI CCWGG 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 81, 157
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 2 cut(s) 115, 377
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 109
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 1 cut(s) 441
Bsh1236I CGCG 2 cut(s) 167, 233
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 303
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 108
BsiHKCI CYCGRG 2 cut(s) 17, 314
BsiSI CCGG 2 cut(s) 12, 52
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 2 cut(s) 115, 377
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 1 cut(s) 343
BsoBI CYCGRG 2 cut(s) 17, 314
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 3 cut(s) 138, 409, 505
BspACI CCGC 5 cut(s) 32, 66, 167, 170, 210
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 274
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 2 cut(s) 167, 233
BspLI GGNNCC 3 cut(s) 16, 56, 305
BspT107I GGYRCC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 109
BssECI CCNNGG 2 cut(s) 81, 157
BssMI GATC 3 cut(s) 138, 409, 505
BssNI GRCGYC 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 415
BstDEI CTNAG 1 cut(s) 282
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 2 cut(s) 167, 233
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 3 cut(s) 141, 412, 508
BstMBI GATC 3 cut(s) 138, 409, 505
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 1 cut(s) 482
BstPAI GACNNNNGTC 1 cut(s) 19
BstSCI CCNGG 3 cut(s) 10, 51, 156
BstUI CGCG 2 cut(s) 167, 233
BstV1I GCAGC 1 cut(s) 441
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
Bsu15I ATCGAT 1 cut(s) 327
BsuRI GGCC 1 cut(s) 343
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 439
BtsIMutI CAGTG 1 cut(s) 116
CaiI CAGNNNCTG 1 cut(s) 300
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 5 cut(s) 4, 14, 55, 154, 176
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 1 cut(s) 58
CviAII CATG 1 cut(s) 479
DdeI CTNAG 1 cut(s) 282
DpnI GATC 3 cut(s) 140, 411, 507
DpnII GATC 3 cut(s) 138, 409, 505
EaeI YGGCCR 1 cut(s) 341
EciI GGCGGA 2 cut(s) 185, 225
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 5 cut(s) 4, 14, 55, 154, 176
Eco88I CYCGRG 2 cut(s) 17, 314
EcoO109I RGGNCCY 2 cut(s) 55, 154
EcoRII CCWGG 1 cut(s) 156
EcoRV GATATC 1 cut(s) 442
FaeI CATG 1 cut(s) 482
FaiI YATR 4 cut(s) 62, 405, 480, 519
FaqI GGGAC 1 cut(s) 557
FatI CATG 1 cut(s) 478
FauI CCCGC 1 cut(s) 39
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 67, 168, 455
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 3 cut(s) 67, 168, 455
FspBI CTAG 1 cut(s) 503
GlaI GCGC 1 cut(s) 232
GluI GCNGC 3 cut(s) 67, 168, 455
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 1 cut(s) 343
HapII CCGG 2 cut(s) 12, 52
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 1 cut(s) 482
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 1 cut(s) 128
HindII GTYRAC 1 cut(s) 128
HinfI GANTC 6 cut(s) 20, 219, 287, 329, 367, 549
HpaII CCGG 2 cut(s) 12, 52
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 6 cut(s) 49, 97, 118, 180, 283, 338
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 3 cut(s) 74, 101, 146
HpyAV CCTTC 5 cut(s) 142, 167, 200, 250, 478
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 1 cut(s) 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 414
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 1 cut(s) 482
HspAI GCGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 3 cut(s) 138, 409, 505
LmnI GCTCC 5 cut(s) 31, 111, 132, 212, 242
LpnPI CCDG 9 cut(s) 25, 65, 122, 143, 170, 201, 280, 286, 320
Lsp1109I GCAGC 1 cut(s) 441
LweI GCATC 1 cut(s) 424
MaeI CTAG 1 cut(s) 503
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 73
MalI GATC 3 cut(s) 140, 411, 507
MboI GATC 3 cut(s) 138, 409, 505
MboII GAAGA 1 cut(s) 193
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 1 cut(s) 446
MlyI GAGTC 2 cut(s) 29, 281
MmeI TCCRAC 3 cut(s) 72, 96, 120
MslI CAYNNNNRTG 1 cut(s) 80
MspI CCGG 2 cut(s) 12, 52
MspR9I CCNGG 3 cut(s) 12, 53, 158
MvaI CCWGG 1 cut(s) 158
MvnI CGCG 2 cut(s) 167, 233
MwoI GCNNNNNNNGC 1 cut(s) 349
NciI CCSGG 2 cut(s) 12, 53
NdeII GATC 3 cut(s) 138, 409, 505
NlaIII CATG 1 cut(s) 482
NlaIV GGNNCC 3 cut(s) 16, 56, 305
NmuCI GTSAC 1 cut(s) 73
NspI RCATGY 1 cut(s) 482
OliI CACNNNNGTG 1 cut(s) 80
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 435
PfeI GAWTC 4 cut(s) 219, 329, 367, 549
PflFI GACNNNGTC 1 cut(s) 53
PkrI GCNGC 3 cut(s) 68, 169, 456
PleI GAGTC 2 cut(s) 28, 281
PpsI GAGTC 2 cut(s) 28, 281
Ppu21I YACGTR 1 cut(s) 415
PpuMI RGGWCCY 2 cut(s) 55, 154
PshAI GACNNNNGTC 1 cut(s) 19
Psp5II RGGWCCY 2 cut(s) 55, 154
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 3 cut(s) 16, 56, 305
PspPI GGNCC 5 cut(s) 4, 14, 55, 154, 176
PspPPI RGGWCCY 2 cut(s) 55, 154
PstNI CAGNNNCTG 1 cut(s) 300
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 80
SatI GCNGC 3 cut(s) 67, 168, 455
Sau3AI GATC 3 cut(s) 138, 409, 505
Sau96I GGNCC 5 cut(s) 4, 14, 55, 154, 176
SchI GAGTC 2 cut(s) 29, 281
ScrFI CCNGG 3 cut(s) 12, 53, 158
SduI GDGCHC 1 cut(s) 108
SfaNI GCATC 1 cut(s) 424
SinI GGWCC 5 cut(s) 4, 14, 55, 154, 176
SmiMI CAYNNNNRTG 1 cut(s) 80
Sse9I AATT 1 cut(s) 446
SsiI CCGC 5 cut(s) 32, 66, 167, 170, 210
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 10, 51, 156
TaiI ACGT 1 cut(s) 417
TaqI TCGA 4 cut(s) 144, 270, 327, 429
TaqII GACCGA 1 cut(s) 21
TasI AATT 1 cut(s) 446
TauI GCSGC 2 cut(s) 69, 170
TfiI GAWTC 4 cut(s) 219, 329, 367, 549
TscAI CASTG 1 cut(s) 116
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 1 cut(s) 454
Tsp45I GTSAC 1 cut(s) 73
TspGWI ACGGA 4 cut(s) 114, 145, 360, 561
TspRI CASTG 1 cut(s) 116
Tth111I GACNNNGTC 1 cut(s) 53
VpaK11BI GGWCC 5 cut(s) 4, 14, 55, 154, 176
XceI RCATGY 1 cut(s) 482
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.