MD05G1345400.v1.1

cellular response to nitrogen starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0028797
Physical Location & Seq
Forward (+)
46503289 .. 46505955
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1345400.v1.1.491

Sequence Viewer

Length: 477 bp
ATGCAGGTGAGTGTGGAGACGGATGCAAGGAGAGACGTTCCTGGCTTTAGAGACAACAAATACCATGTCCACCGTGCTATTCGTCTTGGAGCTTTTGTCAAGCTTATTCAGCTTCGTCTCGAGGTTATAGGAATCACGGATCCTGTCTTTGTGTATTCCAAGAACACCGCTAAACCTCCCAATGAGATCTTGATGTCTGCAATTGACAAGGATGAGGAAAATAAGGATGAAGATGGATTTCTTCACTTGATATACACTAATGAAGAGGAAGGAGTACCAATTCCAGCGAAGAAAGCTGCCACAAGGCACAAAAGTCCTTGCAGATTCAAGAGGAGGAAGAAAATCGGAATGATCCAGTTTTCAACTCCCATTGGTCTGTTAGACTCCTGGAACCTGATCAATACAGGCATAGCTGTTTTTTCATCTATGCGTTTGATTTATGCTCAGTGGTTGTTTGGAGGCGGACAAACCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.95

Weight (kDa)

9.41

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 3 - 89 2.3e-09 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028797)

Species Orthologous Gene IDs
malus_domestica MD05G1345400.v1.1
pyrus_communis pycom05g31430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 168, 462
AclWI GGATC 3 cut(s) 134, 147, 346
AfaI GTAC 1 cut(s) 276
AgsI TTSAA 2 cut(s) 328, 363
AjnI CCWGG 2 cut(s) 40, 386
AluBI AGCT 5 cut(s) 92, 103, 112, 296, 413
AluI AGCT 5 cut(s) 92, 103, 112, 296, 413
Alw26I GTCTC 4 cut(s) 11, 27, 45, 122
AlwI GGATC 3 cut(s) 134, 147, 346
Ama87I CYCGRG 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 296
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 119
BamHI GGATCC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 283
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 2 cut(s) 42, 388
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 4 cut(s) 11, 27, 45, 122
BfaI CTAG 1 cut(s) 475
BglII AGATCT 1 cut(s) 186
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme1390I CCNGG 2 cut(s) 42, 388
BmeT110I CYCGRG 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 141, 392
BmrFI CCNGG 2 cut(s) 42, 388
BmsI GCATC 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bse1I ACTGG 1 cut(s) 355
BseBI CCWGG 2 cut(s) 42, 388
BseGI GGATG 3 cut(s) 28, 217, 232
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 346
BseXI GCAGC 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 119
BsmAI GTCTC 4 cut(s) 11, 27, 45, 122
BsmBI CGTCTC 3 cut(s) 11, 27, 122
BsoBI CYCGRG 1 cut(s) 119
Bsp143I GATC 4 cut(s) 139, 186, 351, 396
BspACI CCGC 2 cut(s) 168, 462
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 141, 392
BspPI GGATC 3 cut(s) 134, 147, 346
BsrI ACTGG 1 cut(s) 355
BssMI GATC 4 cut(s) 139, 186, 351, 396
Bst2UI CCWGG 2 cut(s) 42, 388
Bst4CI ACNGT 1 cut(s) 74
Bst6I CTCTTC 1 cut(s) 258
BstDEI CTNAG 1 cut(s) 444
BstF5I GGATG 3 cut(s) 28, 217, 232
BstKTI GATC 4 cut(s) 142, 189, 354, 399
BstMAI GTCTC 4 cut(s) 11, 27, 45, 122
BstMBI GATC 4 cut(s) 139, 186, 351, 396
BstMWI GCNNNNNNNGC 2 cut(s) 109, 293
BstNI CCWGG 2 cut(s) 42, 388
BstSCI CCNGG 2 cut(s) 40, 386
BstV1I GCAGC 1 cut(s) 283
BstX2I RGATCY 2 cut(s) 139, 186
BstYI RGATCY 2 cut(s) 139, 186
BtsCI GGATG 3 cut(s) 28, 217, 232
BtsIMutI CAGTG 1 cut(s) 452
Csp6I GTAC 1 cut(s) 275
CviAII CATG 1 cut(s) 65
CviJI RGCY 6 cut(s) 45, 92, 103, 112, 296, 413
CviKI_1 RGCY 6 cut(s) 45, 92, 103, 112, 296, 413
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 1 cut(s) 444
DpnI GATC 4 cut(s) 141, 188, 353, 398
DpnII GATC 4 cut(s) 139, 186, 351, 396
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
EciI GGCGGA 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 40, 386
Esp3I CGTCTC 3 cut(s) 11, 27, 122
FaeI CATG 1 cut(s) 68
FaiI YATR 6 cut(s) 66, 128, 253, 410, 428, 441
FatI CATG 1 cut(s) 64
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 3 cut(s) 35, 224, 239
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 1 cut(s) 475
GluI GCNGC 1 cut(s) 297
Hin1II CATG 1 cut(s) 68
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 3 cut(s) 132, 324, 383
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 1 cut(s) 347
Hpy188III TCNNGA 3 cut(s) 119, 190, 328
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 1 cut(s) 263
HpyCH4III ACNGT 1 cut(s) 74
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 4 cut(s) 4, 26, 200, 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 293
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 1 cut(s) 68
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 4 cut(s) 139, 186, 351, 396
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 9 cut(s) 27, 54, 156, 297, 368, 373, 390, 400, 407
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 475
MaeII ACGT 1 cut(s) 36
MalI GATC 4 cut(s) 141, 188, 353, 398
MboI GATC 4 cut(s) 139, 186, 351, 396
MboII GAAGA 5 cut(s) 233, 242, 275, 301, 349
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 2 cut(s) 139, 186
MluCI AATT 2 cut(s) 201, 279
MlyI GAGTC 1 cut(s) 377
MnlI CCTC 7 cut(s) 115, 186, 208, 259, 324, 327, 452
MspR9I CCNGG 2 cut(s) 42, 388
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 2 cut(s) 42, 388
MwoI GCNNNNNNNGC 2 cut(s) 109, 293
NdeII GATC 4 cut(s) 139, 186, 351, 396
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 2 cut(s) 141, 392
PaeR7I CTCGAG 1 cut(s) 119
PfeI GAWTC 2 cut(s) 132, 324
PfoI TCCNGGA 1 cut(s) 386
PkrI GCNGC 1 cut(s) 298
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
Psp6I CCWGG 2 cut(s) 40, 386
PspGI CCWGG 2 cut(s) 40, 386
PspN4I GGNNCC 2 cut(s) 141, 392
PsuI RGATCY 2 cut(s) 139, 186
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 4 cut(s) 139, 186, 351, 396
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 42, 388
SfaNI GCATC 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 2 cut(s) 201, 279
SsiI CCGC 2 cut(s) 168, 462
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 2 cut(s) 40, 386
TaaI ACNGT 1 cut(s) 74
TaiI ACGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 120
TasI AATT 2 cut(s) 201, 279
TfiI GAWTC 2 cut(s) 132, 324
TscAI CASTG 1 cut(s) 452
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 3 cut(s) 243, 276, 411
TspGWI ACGGA 2 cut(s) 35, 152
TspRI CASTG 1 cut(s) 452
XhoI CTCGAG 1 cut(s) 119
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.