Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0028828

Physical Location & Seq

Genomic Coordinates Reverse (-)

46813387 .. 46813878

Gene Structure 492 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1351000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 387 bp

ATGTTCCATCTCGTTGAGATAGTTAAACATGAAACCATTGAAGCATTTGTTTTGTTGCTCTCGCCATATGCACCTCATATGGCTGAGGAGCTTTGGTTTCGCTTGGGACACTCCGAATCTCTGGCATATGAGCCTTTTCCGAAGACGGATCCTGCTTACTTGAAGGAATCTACTAGTGTCCTCCCAGTTCAGATCAACGGCAAGACGAGGGGTACGATCCAGGTTGAAGAGACCTGTTCGGAGGAGGATGCCTTCCAGCTTGCATCACAAGATGAAAAACTCTCCAAATACCTGGATGGGAAGTCAATTAAAAAGAGAATTTTTGTTCCTAGCAAGATCTTGAATGTTATTCTGGACCGTGGAAACGTCAAGGCGGCTGTCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004823 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006429 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032543 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140053 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1 tRNA-synt_1_2

Protein Analysis

129

Amino Acids

14.52

Weight (kDa)

6.08

Isoelectric Point (pI)

49.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Anticodon_1	PF08264	10 - 76	1.4e-12	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0028828)

Species	Orthologous Gene IDs
malus_domestica	MD05G1351000.v1.1
pyrus_communis	pycom05g31830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	374
AclWI	GGATC	3 cut(s)	143, 156, 211
AcsI	RAATTY	1 cut(s)	318
AfaI	GTAC	1 cut(s)	214
AgsI	TTSAA	4 cut(s)	41, 163, 227, 343
AhlI	ACTAGT	1 cut(s)	173
AjnI	CCWGG	2 cut(s)	219, 291
AluBI	AGCT	2 cut(s)	91, 259
AluI	AGCT	2 cut(s)	91, 259
Alw26I	GTCTC	1 cut(s)	224
AlwI	GGATC	3 cut(s)	143, 156, 211
ApoI	RAATTY	1 cut(s)	318
AspS9I	GGNCC	1 cut(s)	355
AvaII	GGWCC	1 cut(s)	355
BamHI	GGATCC	1 cut(s)	148
BbsI	GAAGAC	1 cut(s)	149
BbvCI	CCTCAGC	1 cut(s)	84
BccI	CCATC	2 cut(s)	15, 290
BceAI	ACGGC	1 cut(s)	214
BciT130I	CCWGG	2 cut(s)	221, 293
BcoDI	GTCTC	1 cut(s)	224
BcuI	ACTAGT	1 cut(s)	173
BfaI	CTAG	2 cut(s)	174, 330
BglII	AGATCT	1 cut(s)	336
BisI	GCNGC	1 cut(s)	375
BlsI	GCNGC	1 cut(s)	376
Bme1390I	CCNGG	2 cut(s)	221, 293
Bme18I	GGWCC	1 cut(s)	355
BmgT120I	GGNCC	1 cut(s)	355
BmiI	GGNNCC	1 cut(s)	150
BmrFI	CCNGG	2 cut(s)	221, 293
BmrI	ACTGGG	1 cut(s)	179
BmsI	GCATC	2 cut(s)	238, 272
BmuI	ACTGGG	1 cut(s)	179
BpiI	GAAGAC	1 cut(s)	149
Bpu10I	CCTNAGC	1 cut(s)	84
BsaI	GGTCTC	1 cut(s)	224
BsaJI	CCNNGG	1 cut(s)	358
BsaXI	ACNNNNNCTCC	2 cut(s)	80, 110
Bse1I	ACTGG	1 cut(s)	185
BseBI	CCWGG	2 cut(s)	221, 293
BseDI	CCNNGG	1 cut(s)	358
BseGI	GGATG	2 cut(s)	253, 301
BseMII	CTCAG	1 cut(s)	75
BseNI	ACTGG	1 cut(s)	185
BseRI	GAGGAG	2 cut(s)	101, 257
BslFI	GGGAC	1 cut(s)	120
BsmAI	GTCTC	1 cut(s)	224
BsmFI	GGGAC	1 cut(s)	120
Bso31I	GGTCTC	1 cut(s)	224
Bsp143I	GATC	4 cut(s)	148, 192, 216, 336
BspACI	CCGC	1 cut(s)	374
BspCNI	CTCAG	1 cut(s)	76
BspLI	GGNNCC	1 cut(s)	150
BspPI	GGATC	3 cut(s)	143, 156, 211
BspTNI	GGTCTC	1 cut(s)	224
BsrI	ACTGG	1 cut(s)	185
BssECI	CCNNGG	1 cut(s)	358
BssMI	GATC	4 cut(s)	148, 192, 216, 336
Bst2UI	CCWGG	2 cut(s)	221, 293
Bst4CI	ACNGT	1 cut(s)	359
Bst6I	CTCTTC	1 cut(s)	222
BstC8I	GCNNGC	1 cut(s)	261
BstDEI	CTNAG	1 cut(s)	84
BstDSI	CCRYGG	1 cut(s)	358
BstF5I	GGATG	2 cut(s)	253, 301
BstKTI	GATC	4 cut(s)	151, 195, 219, 339
BstMAI	GTCTC	1 cut(s)	224
BstMBI	GATC	4 cut(s)	148, 192, 216, 336
BstNI	CCWGG	2 cut(s)	221, 293
BstSCI	CCNGG	2 cut(s)	219, 291
BstV2I	GAAGAC	1 cut(s)	149
BstX2I	RGATCY	2 cut(s)	148, 336
BstXI	CCANNNNNNTGG	1 cut(s)	292
BstYI	RGATCY	2 cut(s)	148, 336
BtgI	CCRYGG	1 cut(s)	358
BtsCI	GGATG	2 cut(s)	253, 301
Cac8I	GCNNGC	1 cut(s)	261
Cfr13I	GGNCC	1 cut(s)	355
Csp6I	GTAC	1 cut(s)	213
CviAII	CATG	1 cut(s)	29
CviJI	RGCY	5 cut(s)	83, 91, 133, 259, 377
CviKI_1	RGCY	5 cut(s)	83, 91, 133, 259, 377
CviQI	GTAC	1 cut(s)	213
DdeI	CTNAG	1 cut(s)	84
DpnI	GATC	4 cut(s)	150, 194, 218, 338
DpnII	GATC	4 cut(s)	148, 192, 216, 336
Eam1104I	CTCTTC	1 cut(s)	222
EarI	CTCTTC	1 cut(s)	222
Eco31I	GGTCTC	1 cut(s)	224
Eco47I	GGWCC	1 cut(s)	355
EcoRII	CCWGG	2 cut(s)	219, 291
FaeI	CATG	1 cut(s)	32
FaiI	YATR	7 cut(s)	30, 67, 69, 78, 80, 127, 129
FaqI	GGGAC	1 cut(s)	120
FatI	CATG	1 cut(s)	28
FauNDI	CATATG	3 cut(s)	67, 78, 127
Fnu4HI	GCNGC	1 cut(s)	375
FokI	GGATG	2 cut(s)	260, 308
Fsp4HI	GCNGC	1 cut(s)	375
FspBI	CTAG	2 cut(s)	174, 330
GluI	GCNGC	1 cut(s)	375
Hin1II	CATG	1 cut(s)	32
HinfI	GANTC	2 cut(s)	116, 167
Hpy188I	TCNGA	5 cut(s)	115, 141, 192, 241, 383
Hpy188III	TCNNGA	2 cut(s)	340, 353
HpyAV	CCTTC	2 cut(s)	157, 262
HpyCH4III	ACNGT	1 cut(s)	359
HpyCH4IV	ACGT	1 cut(s)	366
HpyCH4V	TGCA	2 cut(s)	71, 263
HpyF3I	CTNAG	1 cut(s)	84
HpySE526I	ACGT	1 cut(s)	366
Hsp92II	CATG	1 cut(s)	32
Kzo9I	GATC	4 cut(s)	148, 192, 216, 336
LmnI	GCTCC	1 cut(s)	88
LweI	GCATC	2 cut(s)	238, 272
MaeI	CTAG	2 cut(s)	174, 330
MaeII	ACGT	1 cut(s)	366
MalI	GATC	4 cut(s)	150, 194, 218, 338
MboI	GATC	4 cut(s)	148, 192, 216, 336
MboII	GAAGA	2 cut(s)	154, 239
MflI	RGATCY	2 cut(s)	148, 336
MluCI	AATT	2 cut(s)	306, 318
MnlI	CCTC	6 cut(s)	79, 84, 191, 201, 235, 238
MseI	TTAA	2 cut(s)	24, 309
MspR9I	CCNGG	2 cut(s)	221, 293
MvaI	CCWGG	2 cut(s)	221, 293
NdeI	CATATG	3 cut(s)	67, 78, 127
NdeII	GATC	4 cut(s)	148, 192, 216, 336
NlaIII	CATG	1 cut(s)	32
NlaIV	GGNNCC	1 cut(s)	150
PcsI	WCGNNNNNNNCGW	1 cut(s)	212
PfeI	GAWTC	2 cut(s)	116, 167
PkrI	GCNGC	1 cut(s)	376
Psp6I	CCWGG	2 cut(s)	219, 291
PspGI	CCWGG	2 cut(s)	219, 291
PspN4I	GGNNCC	1 cut(s)	150
PspPI	GGNCC	1 cut(s)	355
PsuI	RGATCY	2 cut(s)	148, 336
RsaI	GTAC	1 cut(s)	214
RsaNI	GTAC	1 cut(s)	213
SaqAI	TTAA	2 cut(s)	24, 309
SatI	GCNGC	1 cut(s)	375
Sau3AI	GATC	4 cut(s)	148, 192, 216, 336
Sau96I	GGNCC	1 cut(s)	355
ScrFI	CCNGG	2 cut(s)	221, 293
SetI	ASST	7 cut(s)	76, 93, 225, 236, 261, 294, 369
SfaNI	GCATC	2 cut(s)	238, 272
SinI	GGWCC	1 cut(s)	355
SpeI	ACTAGT	1 cut(s)	173
Sse9I	AATT	2 cut(s)	306, 318
SsiI	CCGC	1 cut(s)	374
SspMI	CTAG	2 cut(s)	174, 330
StyD4I	CCNGG	2 cut(s)	219, 291
TaaI	ACNGT	1 cut(s)	359
TaiI	ACGT	1 cut(s)	369
TasI	AATT	2 cut(s)	306, 318
TauI	GCSGC	1 cut(s)	377
TfiI	GAWTC	2 cut(s)	116, 167
Tru1I	TTAA	2 cut(s)	24, 309
Tru9I	TTAA	2 cut(s)	24, 309
TspDTI	ATGAA	2 cut(s)	45, 288
TspGWI	ACGGA	1 cut(s)	161
VpaK11BI	GGWCC	1 cut(s)	355
XapI	RAATTY	1 cut(s)	318
XspI	CTAG	2 cut(s)	174, 330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.