MD06G1203400.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013982
Physical Location & Seq
Forward (+)
33802344 .. 33805578
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1203400.v1.1.491

Sequence Viewer

Length: 1773 bp
ATGTTTACCGCGATTACAAATCGAAGAGACACCTTCACTCTCTATATAAACTCAGTCTCATTTCACTTCTCCCACCTCTACCATCCCTCTCAGCTTTTAATTTCCCACCACTTGGGTTTCTTGTTTCCTAAGCTTTGCCTTTTCATTTTCTCGTTCATTTTCCGAGTAACCATGGAATCCAACCCTCTCTTGATTCCACCAACTGGGTTATCATCATTTTTGAGCTTGGAGTTGTTCATCTGTCTCTTCCTCTTCGTCTTTGTTTTTGGATTTTGGCTCTCACCCGGTGGATTGGCTTGGGCATTGTCCAAATTTAAAGGTCAAATCCATTCGAAAACCACCATTCCCGGTCCGTCCGGCCTTCCTCTTCTAGGGTTGGTCTTGTCCTTCACTGGCTCTCTCACTCACAGAGTTCTAGCCAAGCTTGCTGAGACCTCAAAGGCCAAACCTCTAATGGCGTTCTCAGTAGGGTTTACTCGCTTTGTCATCTCCAGTCACCCTGATACAGCGAAAGAGCTCTTGAACAGCACCGCCTTCGCTGACCGACCCATCAAAGAGTCGGCTTACGAGCTTTTGTTCCACAAAGCAATGGGGTTTGCCCCTTTTGGTGAGTATTGGAGGAACTTGAGGAGAATCTCCTCCACCCATTTGTTCCACCCGCAGCGAATTGCTAGTTTTGGGTTGTTTCGCGAGAGTATCGGGCGAAAAATGGTGGGGGAGATGAAGAGTTTTATGAAGAGGAGCGATGAGGTGGAGGTTAGGAAAGTGTTGCATTTTGGGTCTTTGAACAGTGTGATGATGAGTGTGTTTGGGAGGAGCTATGAGTTTGGTGGAGAATTAGGGTGTGGAAATGGTGAGGGTTTAGAGCTTGAGGGGTTGGTTAGTGAGGGGTATGAGTTGCTTGGGATTTTCAATTGGAGTGATCACTTTCCTTTTTTGGGGTTTTTGGACTTGCAAGGTGTGAGGAGGAGGTGTAAAAAATTGGTGGCAAAGGTGAATGCTTTTGTTGGGAAAATCATAGAGGAGCATAGGGTGAAGAGGGATGAAAATTATGGAGTTTTGAGTGCTGATCATGAAAGTGGTGATTTTGTTGACGTGCTTCTTGATCTGGAGAAGGAGAACAAACTCAGTGACTCTGATATGATTGCAGTTCTGTGGGAAATGATCTTCAGAGGTACTGATACAGTGGCAATACTCCTAGAGTGGATTCTAGCAAGAATGGTTCTCCACCCAGATATCCAAGCCAAGGCCCAATCGGAGATCGACGCCGTTGTAGGCACTCCGGCACGATCTGTATCCAATTTCGACCTCCCCAAGCTTCCTTACCTCCATGCCATAGTCAAAGAAACCCTAAGAATGCATCCACCAGGACCCCTCTTGTCCTGGGCCAGACTTGCCATCCATGACACTCACATAGGCCACCACTTCATCCCGGCCGGCACCACTGCCATGGTCAACATGTTTGCAATCACACACGATGAGGAAATCTGGTCAAACCCTAATGAGTTCAAACCAGAAAGATTGGTTAGTGAAGATGTTCTAATCATGGGGTCTGATTTAAGGCTGGCTCCCTTTGGGTCTGGAAGAAGGGTTTGTCCTGGAAAAGCCATGGGATTGGCTACTGTTGAGCTTTGGCTTGCTCAGTTGCTTCAGAGCTTCAAGTGGGTCCCTTGTGATGATCAGTCAGGTGTGGATTTGTCTGAGACCTTGAAGCTTTCTTTGGAAATGAAGAACTCTCTTGTCTGCAAAGCTGTTCCAAGGGTTGTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

591

Amino Acids

66.24

Weight (kDa)

7.37

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 116 - 578 1.7e-84 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74110
fragaria_vesca FvH4_5g13590
malus_domestica MD06G1203400.v1.1 MD14G1214200.v1.1
prunus_persica Prupe.5G207500_v2.0.a1
pyrus_communis pycom06g18140 pycom14g17760
rosa_chinensis RchiOBHm_Chr7g0183351
rosa_laevigata RLG00000005054
rosa_multiflora Rmu_sc0008602.1_g000003
rosa_roxburghii Rroxscaffold_3G00270520
rosa_rugosa Rorug06G0457000
rosa_samantha Rh7AG058400 Rh7BG058400 Rh7CG060000 Rh7DG058100
rosa_wichuraiana Rw7G004800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1439
AccB7I CCANNNNNTGG 2 cut(s) 112, 203
AccII CGCG 2 cut(s) 11, 690
AciI CCGC 3 cut(s) 9, 531, 659
AcoI YGGCCR 1 cut(s) 1434
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 2 cut(s) 1153, 1634
AcyI GRCGYC 1 cut(s) 1266
AdeI CACNNNGTG 1 cut(s) 287
AfaI GTAC 1 cut(s) 1177
AfiI CCNNNNNNNGG 5 cut(s) 112, 203, 371, 938, 1246
AflIII ACRYGT 1 cut(s) 1458
AgsI TTSAA 6 cut(s) 523, 787, 913, 1510, 1660, 1711
AjiI CACGTC 1 cut(s) 1096
AjnI CCWGG 3 cut(s) 1366, 1382, 1597
AjuI GAANNNNNNNTTGG 2 cut(s) 1703, 1735
Alw21I GWGCWC 1 cut(s) 519
Alw26I GTCTC 5 cut(s) 21, 61, 248, 425, 1697
AoxI GGCC 6 cut(s) 358, 441, 1248, 1386, 1417, 1434
ApeKI GCWGC 1 cut(s) 661
ApoI RAATTY 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 1536
AspS9I GGNCC 5 cut(s) 350, 1249, 1370, 1386, 1666
AsuC2I CCSGG 3 cut(s) 285, 348, 1433
AsuHPI GGTGA 7 cut(s) 273, 488, 620, 866, 1006, 1045, 1094
AsuII TTCGAA 1 cut(s) 332
AvaII GGWCC 3 cut(s) 350, 1370, 1666
BanI GGYRCC 1 cut(s) 1439
BanII GRGCYC 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 519
BbvI GCAGC 1 cut(s) 673
BccI CCATC 3 cut(s) 90, 557, 1406
BceAI ACGGC 1 cut(s) 1253
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BciT130I CCWGG 3 cut(s) 1368, 1384, 1599
BciVI GTATCC 1 cut(s) 1306
BclI TGATCA 3 cut(s) 922, 1069, 1678
BcnI CCSGG 3 cut(s) 285, 348, 1433
BcoDI GTCTC 5 cut(s) 21, 61, 248, 425, 1697
BfaI CTAG 5 cut(s) 371, 416, 672, 1199, 1211
BfuI GTATCC 1 cut(s) 1306
BisI GCNGC 1 cut(s) 662
BlsI GCNGC 1 cut(s) 663
Bme1390I CCNGG 6 cut(s) 285, 348, 1368, 1384, 1433, 1599
Bme18I GGWCC 3 cut(s) 350, 1370, 1666
BmgBI CACGTC 1 cut(s) 1096
BmgT120I GGNCC 5 cut(s) 350, 1249, 1370, 1386, 1666
BmiI GGNNCC 5 cut(s) 1372, 1441, 1569, 1667, 1668
BmrFI CCNGG 6 cut(s) 285, 348, 1368, 1384, 1433, 1599
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 1 cut(s) 1369
BmuI ACTGGG 1 cut(s) 213
BpmI CTGGAG 2 cut(s) 475, 1130
Bpu10I CCTNAGC 1 cut(s) 129
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 2 cut(s) 646, 890
BpuMI CCSGG 3 cut(s) 285, 348, 1433
BsaBI GATNNNNATC 1 cut(s) 1294
BsaHI GRCGYC 1 cut(s) 1266
BsaI GGTCTC 2 cut(s) 425, 1697
BsaJI CCNNGG 6 cut(s) 171, 1245, 1383, 1449, 1608, 1757
BsaXI ACNNNNNCTCC 2 cut(s) 958, 988
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 203, 371, 938, 1246
Bse118I RCCGGY 1 cut(s) 1436
Bse1I ACTGG 3 cut(s) 208, 397, 492
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 1294
BseBI CCWGG 3 cut(s) 1368, 1384, 1599
BseDI CCNNGG 6 cut(s) 171, 1245, 1383, 1449, 1608, 1757
BseGI GGATG 5 cut(s) 82, 1048, 1360, 1398, 1428
BseJI GATNNNNATC 1 cut(s) 1294
BseLI CCNNNNNNNGG 5 cut(s) 112, 203, 371, 938, 1246
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 7 cut(s) 66, 104, 420, 477, 1141, 1655, 1692
BseNI ACTGG 3 cut(s) 208, 397, 492
BseRI GAGGAG 7 cut(s) 628, 643, 754, 829, 979, 982, 1037
BseX3I CGGCCG 1 cut(s) 1434
BseXI GCAGC 1 cut(s) 673
Bsh1236I CGCG 2 cut(s) 11, 690
Bsh1285I CGRYCG 1 cut(s) 1437
BshFI GGCC 6 cut(s) 360, 443, 1250, 1388, 1419, 1436
BshNI GGYRCC 1 cut(s) 1439
BsiEI CGRYCG 1 cut(s) 1437
BsiHKAI GWGCWC 1 cut(s) 519
BsiSI CCGG 6 cut(s) 285, 348, 357, 1283, 1433, 1437
BslFI GGGAC 1 cut(s) 1652
BslI CCNNNNNNNGG 5 cut(s) 112, 203, 371, 938, 1246
BsmAI GTCTC 5 cut(s) 21, 61, 248, 425, 1697
BsmFI GGGAC 1 cut(s) 1652
BsmI GAATGC 2 cut(s) 1003, 1362
BsnI GGCC 6 cut(s) 360, 443, 1250, 1388, 1419, 1436
Bso31I GGTCTC 2 cut(s) 425, 1697
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 519
Bsp143I GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
Bsp19I CCATGG 3 cut(s) 171, 1449, 1608
Bsp68I TCGCGA 1 cut(s) 690
BspACI CCGC 3 cut(s) 9, 531, 659
BspANI GGCC 6 cut(s) 360, 443, 1250, 1388, 1419, 1436
BspCNI CTCAG 7 cut(s) 65, 103, 421, 476, 1140, 1654, 1693
BspFNI CGCG 2 cut(s) 11, 690
BspHI TCATGA 1 cut(s) 1072
BspLI GGNNCC 5 cut(s) 1372, 1441, 1569, 1667, 1668
BspT104I TTCGAA 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 1439
BspTNI GGTCTC 2 cut(s) 425, 1697
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 1 cut(s) 1436
BsrI ACTGG 3 cut(s) 208, 397, 492
BssAI RCCGGY 1 cut(s) 1436
BssECI CCNNGG 6 cut(s) 171, 1245, 1383, 1449, 1608, 1757
BssMI GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
BssNI GRCGYC 1 cut(s) 1266
BssT1I CCWWGG 5 cut(s) 171, 1245, 1449, 1608, 1757
Bst2UI CCWGG 3 cut(s) 1368, 1384, 1599
Bst4CI ACNGT 3 cut(s) 791, 1186, 1624
Bst6I CTCTTC 7 cut(s) 19, 251, 257, 372, 719, 731, 1031
BstACI GRCGYC 1 cut(s) 1266
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 4 cut(s) 426, 1438, 1566, 1638
BstDEI CTNAG 9 cut(s) 52, 90, 129, 429, 463, 1127, 1352, 1641, 1701
BstDSI CCRYGG 3 cut(s) 171, 1449, 1608
BstENI CCTNNNNNAGG 1 cut(s) 369
BstF5I GGATG 5 cut(s) 82, 1048, 1360, 1398, 1428
BstFNI CGCG 2 cut(s) 11, 690
BstKTI GATC 7 cut(s) 925, 1072, 1108, 1167, 1263, 1292, 1681
BstMAI GTCTC 5 cut(s) 21, 61, 248, 425, 1697
BstMBI GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
BstMCI CGRYCG 1 cut(s) 1437
BstMWI GCNNNNNNNGC 2 cut(s) 425, 1394
BstNI CCWGG 3 cut(s) 1368, 1384, 1599
BstNSI RCATGY 1 cut(s) 1462
BstSCI CCNGG 6 cut(s) 283, 346, 1366, 1382, 1431, 1597
BstUI CGCG 2 cut(s) 11, 690
BstV1I GCAGC 1 cut(s) 673
BstXI CCANNNNNNTGG 2 cut(s) 1450, 1615
BstZI CGGCCG 1 cut(s) 1434
BsuI GTATCC 1 cut(s) 1306
BsuRI GGCC 6 cut(s) 360, 443, 1250, 1388, 1419, 1436
BtgI CCRYGG 3 cut(s) 171, 1449, 1608
BtgZI GCGATG 1 cut(s) 759
BtrI CACGTC 1 cut(s) 1096
BtsCI GGATG 5 cut(s) 82, 1048, 1360, 1398, 1428
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 5 cut(s) 390, 796, 1135, 1191, 1443
BtuMI TCGCGA 1 cut(s) 690
Cac8I GCNNGC 4 cut(s) 426, 1438, 1566, 1638
CciI TCATGA 1 cut(s) 1072
Cfr10I RCCGGY 1 cut(s) 1436
Cfr13I GGNCC 5 cut(s) 350, 1249, 1370, 1386, 1666
CpoI CGGWCCG 1 cut(s) 350
CseI GACGC 1 cut(s) 1274
Csp6I GTAC 1 cut(s) 1176
CspI CGGWCCG 1 cut(s) 350
CviAII CATG 8 cut(s) 172, 1073, 1331, 1403, 1450, 1459, 1546, 1609
CviQI GTAC 1 cut(s) 1176
DdeI CTNAG 9 cut(s) 52, 90, 129, 429, 463, 1127, 1352, 1641, 1701
DpnI GATC 7 cut(s) 924, 1071, 1107, 1166, 1262, 1291, 1680
DpnII GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
DraI TTTAAA 1 cut(s) 316
DraIII CACNNNGTG 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 1434
EagI CGGCCG 1 cut(s) 1434
Eam1104I CTCTTC 7 cut(s) 19, 251, 257, 372, 719, 731, 1031
EarI CTCTTC 7 cut(s) 19, 251, 257, 372, 719, 731, 1031
Ecl136II GAGCTC 1 cut(s) 517
EclXI CGGCCG 1 cut(s) 1434
Eco130I CCWWGG 5 cut(s) 171, 1245, 1449, 1608, 1757
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 2 cut(s) 425, 1697
Eco32I GATATC 1 cut(s) 1237
Eco47I GGWCC 3 cut(s) 350, 1370, 1666
Eco52I CGGCCG 1 cut(s) 1434
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 2 cut(s) 1153, 1634
EcoICRI GAGCTC 1 cut(s) 517
EcoNI CCTNNNNNAGG 1 cut(s) 369
EcoO109I RGGNCCY 2 cut(s) 1370, 1666
EcoRII CCWGG 3 cut(s) 1366, 1382, 1597
EcoRV GATATC 1 cut(s) 1237
EcoT14I CCWWGG 5 cut(s) 171, 1245, 1449, 1608, 1757
EcoT22I ATGCAT 1 cut(s) 1362
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 5 cut(s) 171, 1245, 1449, 1608, 1757
FaeI CATG 8 cut(s) 175, 1076, 1334, 1406, 1453, 1462, 1549, 1612
FaqI GGGAC 1 cut(s) 1652
FatI CATG 8 cut(s) 171, 1072, 1330, 1402, 1449, 1458, 1545, 1608
FauI CCCGC 1 cut(s) 666
FbaI TGATCA 3 cut(s) 922, 1069, 1678
Fnu4HI GCNGC 1 cut(s) 662
FokI GGATG 5 cut(s) 69, 1055, 1347, 1385, 1415
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 1 cut(s) 662
FspBI CTAG 5 cut(s) 371, 416, 672, 1199, 1211
GluI GCNGC 1 cut(s) 662
GsuI CTGGAG 2 cut(s) 475, 1130
HaeIII GGCC 6 cut(s) 360, 443, 1250, 1388, 1419, 1436
HapII CCGG 6 cut(s) 285, 348, 357, 1283, 1433, 1437
HgaI GACGC 1 cut(s) 1274
Hin1I GRCGYC 1 cut(s) 1266
Hin1II CATG 8 cut(s) 175, 1076, 1334, 1406, 1453, 1462, 1549, 1612
HincII GTYRAC 2 cut(s) 1093, 1456
HindII GTYRAC 2 cut(s) 1093, 1456
HindIII AAGCTT 4 cut(s) 131, 422, 1316, 1712
HinfI GANTC 6 cut(s) 176, 193, 557, 633, 1133, 1207
HpaII CCGG 6 cut(s) 285, 348, 357, 1283, 1433, 1437
HphI GGTGA 7 cut(s) 273, 488, 620, 866, 1006, 1045, 1094
Hpy166II GTNNAC 4 cut(s) 6, 474, 1093, 1456
Hpy188I TCNGA 7 cut(s) 164, 1138, 1172, 1258, 1555, 1653, 1702
Hpy188III TCNNGA 7 cut(s) 190, 520, 689, 1073, 1103, 1109, 1581
Hpy8I GTNNAC 4 cut(s) 6, 474, 1093, 1456
Hpy99I CGWCG 1 cut(s) 1268
HpyAV CCTTC 6 cut(s) 43, 371, 397, 544, 1108, 1581
HpyCH4III ACNGT 3 cut(s) 791, 1186, 1624
HpyCH4IV ACGT 1 cut(s) 1095
HpyCH4V TGCA 6 cut(s) 772, 955, 1148, 1360, 1466, 1746
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 1394
HpyF3I CTNAG 9 cut(s) 52, 90, 129, 429, 463, 1127, 1352, 1641, 1701
HpySE526I ACGT 1 cut(s) 1095
Hsp92I GRCGYC 1 cut(s) 1266
Hsp92II CATG 8 cut(s) 175, 1076, 1334, 1406, 1453, 1462, 1549, 1612
KflI GGGWCCC 1 cut(s) 1666
KroI GCCGGC 1 cut(s) 1436
KroNI GCCGGC 1 cut(s) 1438
Ksp22I TGATCA 3 cut(s) 922, 1069, 1678
Kzo9I GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
LmnI GCTCC 4 cut(s) 741, 816, 1024, 1573
Lsp1109I GCAGC 1 cut(s) 673
LweI GCATC 1 cut(s) 1369
MaeI CTAG 5 cut(s) 371, 416, 672, 1199, 1211
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 3 cut(s) 166, 494, 1130
MalI GATC 7 cut(s) 924, 1071, 1107, 1166, 1262, 1291, 1680
MboI GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
MfeI CAATTG 1 cut(s) 913
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 8 cut(s) 99, 311, 666, 836, 913, 980, 1048, 1300
MlyI GAGTC 2 cut(s) 566, 1127
MmeI TCCRAC 1 cut(s) 204
Mph1103I ATGCAT 1 cut(s) 1362
MroNI GCCGGC 1 cut(s) 1436
MroXI GAANNNNTTC 1 cut(s) 1536
MseI TTAA 3 cut(s) 98, 315, 1559
MslI CAYNNNNRTG 2 cut(s) 1077, 1448
MspI CCGG 6 cut(s) 285, 348, 357, 1283, 1433, 1437
MspR9I CCNGG 6 cut(s) 285, 348, 1368, 1384, 1433, 1599
MunI CAATTG 1 cut(s) 913
Mva1269I GAATGC 2 cut(s) 1003, 1362
MvaI CCWGG 3 cut(s) 1368, 1384, 1599
MvnI CGCG 2 cut(s) 11, 690
MwoI GCNNNNNNNGC 2 cut(s) 425, 1394
NaeI GCCGGC 1 cut(s) 1438
NciI CCSGG 3 cut(s) 285, 348, 1433
NcoI CCATGG 3 cut(s) 171, 1449, 1608
NdeII GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
NgoMIV GCCGGC 1 cut(s) 1436
NlaIII CATG 8 cut(s) 175, 1076, 1334, 1406, 1453, 1462, 1549, 1612
NlaIV GGNNCC 5 cut(s) 1372, 1441, 1569, 1667, 1668
NmuCI GTSAC 2 cut(s) 494, 1130
NruI TCGCGA 1 cut(s) 690
NsiI ATGCAT 1 cut(s) 1362
NspI RCATGY 1 cut(s) 1462
NspV TTCGAA 1 cut(s) 332
PagI TCATGA 1 cut(s) 1072
PciI ACATGT 1 cut(s) 1458
PctI GAATGC 2 cut(s) 1003, 1362
PdiI GCCGGC 1 cut(s) 1438
PdmI GAANNNNTTC 1 cut(s) 1536
PfeI GAWTC 4 cut(s) 176, 193, 633, 1207
PflMI CCANNNNNTGG 2 cut(s) 112, 203
PfoI TCCNGGA 1 cut(s) 1597
PkrI GCNGC 1 cut(s) 663
PleI GAGTC 2 cut(s) 565, 1127
PpsI GAGTC 2 cut(s) 565, 1127
PpuMI RGGWCCY 2 cut(s) 1370, 1666
PscI ACATGT 1 cut(s) 1458
Psp124BI GAGCTC 1 cut(s) 519
Psp5II RGGWCCY 2 cut(s) 1370, 1666
Psp6I CCWGG 3 cut(s) 1366, 1382, 1597
PspGI CCWGG 3 cut(s) 1366, 1382, 1597
PspN4I GGNNCC 5 cut(s) 1372, 1441, 1569, 1667, 1668
PspPI GGNCC 5 cut(s) 350, 1249, 1370, 1386, 1666
PspPPI RGGWCCY 2 cut(s) 1370, 1666
RruI TCGCGA 1 cut(s) 690
RsaI GTAC 1 cut(s) 1177
RsaNI GTAC 1 cut(s) 1176
RseI CAYNNNNRTG 2 cut(s) 1077, 1448
Rsr2I CGGWCCG 1 cut(s) 350
RsrII CGGWCCG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 519
SaqAI TTAA 3 cut(s) 98, 315, 1559
SatI GCNGC 1 cut(s) 662
Sau3AI GATC 7 cut(s) 922, 1069, 1105, 1164, 1260, 1289, 1678
Sau96I GGNCC 5 cut(s) 350, 1249, 1370, 1386, 1666
SchI GAGTC 2 cut(s) 566, 1127
ScrFI CCNGG 6 cut(s) 285, 348, 1368, 1384, 1433, 1599
SduI GDGCHC 1 cut(s) 519
SfaNI GCATC 1 cut(s) 1369
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 3 cut(s) 350, 1370, 1666
SmiMI CAYNNNNRTG 2 cut(s) 1077, 1448
SmlI CTYRAG 2 cut(s) 625, 869
SmoI CTYRAG 2 cut(s) 625, 869
Sse9I AATT 8 cut(s) 99, 311, 666, 836, 913, 980, 1048, 1300
SsiI CCGC 3 cut(s) 9, 531, 659
SspMI CTAG 5 cut(s) 371, 416, 672, 1199, 1211
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 6 cut(s) 283, 346, 1366, 1382, 1431, 1597
StyI CCWWGG 5 cut(s) 171, 1245, 1449, 1608, 1757
TaaI ACNGT 3 cut(s) 791, 1186, 1624
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 4 cut(s) 22, 332, 1263, 1305
TaqII GACCGA 1 cut(s) 558
TasI AATT 8 cut(s) 99, 311, 666, 836, 913, 980, 1048, 1300
TfiI GAWTC 4 cut(s) 176, 193, 633, 1207
Tru1I TTAA 3 cut(s) 98, 315, 1559
Tru9I TTAA 3 cut(s) 98, 315, 1559
TscAI CASTG 5 cut(s) 397, 796, 1135, 1191, 1450
TseFI GTSAC 2 cut(s) 494, 1130
TseI GCWGC 1 cut(s) 661
Tsp45I GTSAC 2 cut(s) 494, 1130
TspDTI ATGAA 9 cut(s) 133, 145, 226, 737, 749, 1059, 1089, 1417, 1742
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 5 cut(s) 397, 796, 1135, 1191, 1450
Van91I CCANNNNNTGG 2 cut(s) 112, 203
VpaK11BI GGWCC 3 cut(s) 350, 1370, 1666
XagI CCTNNNNNAGG 1 cut(s) 369
XapI RAATTY 1 cut(s) 311
XceI RCATGY 1 cut(s) 1462
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 1536
XspI CTAG 5 cut(s) 371, 416, 672, 1199, 1211
Zsp2I ATGCAT 1 cut(s) 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.