MD07G1012300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0028861
Physical Location & Seq
Reverse (-)
1112570 .. 1114222
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1012300.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGGTTCCAAGGATCGGGATGGGGTTCTAGGCGTTGAATGGGTTCCAGATGAGAGAGATGTGACCATGACGTTAGCATGGAGTCAGATGAGCCGTCTCCTTCCTCTGTCGATTTTAGGCCGGCCTGTGGACTGGCTTGGGCTGGGTGCTAGTCTATTCCACGGGCTGGAGTGGATTGACAGGGTCCTAGCCTGGTTTTTTGACTGGGTGCTGGGCTATTCCACCTCCGGTGGAACTGCTCTAAAGATCTTCGGTGGCCTTTCGGCTGTCAATTCTTGTAAAGATTGCCGGAATTCTGAGTCTGGCGAGCTCCATCTCTTTGACAGGTTCTGGTTCAAGATTGAAGAAACTCTCAGGTTCGTTCAGAATGTTTGTTGTTTAGGGGCCAGCCTTTCTGGCGTTGTGAACATGCTTGTCATGGCTTCAAATCACCACGGTATAGGTTTTACAATTGTTGATACTGACAAATTAAAGATTGAGCTTGGAATAATGTCTGGAATGGTGTGCCTGATGCTGCATTGCCTTTTGAGGAGCCTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.0

Weight (kDa)

5.68

Isoelectric Point (pI)

29.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028861)

Species Orthologous Gene IDs
malus_domestica MD07G1012300.v1.1
pyrus_communis pycom07g00990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 166
AclWI GGATC 1 cut(s) 21
AcsI RAATTY 1 cut(s) 292
AfiI CCNNNNNNNGG 3 cut(s) 15, 127, 166
AgsI TTSAA 4 cut(s) 38, 337, 344, 426
AjnI CCWGG 1 cut(s) 191
AluBI AGCT 2 cut(s) 310, 481
AluI AGCT 2 cut(s) 310, 481
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 21
AlwNI CAGNNNCTG 1 cut(s) 330
AoxI GGCC 4 cut(s) 118, 122, 256, 384
ApeKI GCWGC 1 cut(s) 514
ApoI RAATTY 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 184, 384
AsuHPI GGTGA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 1 cut(s) 501
BccI CCATC 2 cut(s) 14, 321
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 3 cut(s) 29, 150, 188
BglI GCCNNNNNGGC 1 cut(s) 396
BglII AGATCT 1 cut(s) 246
BisI GCNGC 1 cut(s) 515
BlsI GCNGC 1 cut(s) 516
Bme1390I CCNGG 1 cut(s) 193
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 184, 384
BmiI GGNNCC 5 cut(s) 7, 45, 185, 385, 533
BmrFI CCNGG 1 cut(s) 193
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 501
BmuI ACTGGG 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 9, 160, 433
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 127, 166
Bse118I RCCGGY 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 137, 209
Bse3DI GCAATG 1 cut(s) 517
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 3 cut(s) 9, 160, 433
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 3 cut(s) 15, 127, 166
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 2 cut(s) 288, 367
BseNI ACTGG 2 cut(s) 137, 209
BseXI GCAGC 1 cut(s) 501
BseYI CCCAGC 2 cut(s) 142, 211
BshFI GGCC 4 cut(s) 120, 124, 258, 386
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 3 cut(s) 121, 228, 289
BslI CCNNNNNNNGG 3 cut(s) 15, 127, 166
BsmAI GTCTC 1 cut(s) 101
BsmBI CGTCTC 1 cut(s) 101
BsnI GGCC 4 cut(s) 120, 124, 258, 386
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 13, 246
BspANI GGCC 4 cut(s) 120, 124, 258, 386
BspCNI CTCAG 2 cut(s) 289, 366
BspLI GGNNCC 5 cut(s) 7, 45, 185, 385, 533
BspPI GGATC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 120
BsrI ACTGG 2 cut(s) 137, 209
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 3 cut(s) 9, 160, 433
BssMI GATC 2 cut(s) 13, 246
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 122, 308, 388
BstDEI CTNAG 2 cut(s) 297, 353
BstDSI CCRYGG 2 cut(s) 160, 433
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 2 cut(s) 16, 249
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 13, 246
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstNI CCWGG 1 cut(s) 193
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 501
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 4 cut(s) 120, 124, 258, 386
BtgI CCRYGG 2 cut(s) 160, 433
BtsCI GGATG 1 cut(s) 25
Cac8I GCNNGC 3 cut(s) 122, 308, 388
CaiI CAGNNNCTG 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 184, 384
CviAII CATG 4 cut(s) 67, 78, 409, 418
DdeI CTNAG 2 cut(s) 297, 353
DpnI GATC 2 cut(s) 15, 248
DpnII GATC 2 cut(s) 13, 246
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 310
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 4 cut(s) 70, 81, 412, 421
FaiI YATR 5 cut(s) 68, 79, 410, 419, 440
FatI CATG 4 cut(s) 66, 77, 408, 417
Fnu4HI GCNGC 1 cut(s) 515
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 312
FseI GGCCGGCC 1 cut(s) 124
Fsp4HI GCNGC 1 cut(s) 515
FspBI CTAG 3 cut(s) 29, 150, 188
GluI GCNGC 1 cut(s) 515
GsaI CCCAGC 2 cut(s) 146, 215
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 4 cut(s) 120, 124, 258, 386
HapII CCGG 3 cut(s) 121, 228, 289
Hin1II CATG 4 cut(s) 70, 81, 412, 421
HinfI GANTC 2 cut(s) 82, 299
HpaII CCGG 3 cut(s) 121, 228, 289
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 130, 406
Hpy188I TCNGA 4 cut(s) 87, 298, 366, 542
Hpy188III TCNNGA 4 cut(s) 17, 47, 337, 495
Hpy8I GTNNAC 2 cut(s) 130, 406
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 1 cut(s) 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpyF3I CTNAG 2 cut(s) 297, 353
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 4 cut(s) 70, 81, 412, 421
KroI GCCGGC 1 cut(s) 120
KroNI GCCGGC 1 cut(s) 122
Kzo9I GATC 2 cut(s) 13, 246
LmnI GCTCC 2 cut(s) 315, 531
Lsp1109I GCAGC 1 cut(s) 501
LweI GCATC 1 cut(s) 501
MaeI CTAG 3 cut(s) 29, 150, 188
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 1 cut(s) 61
MalI GATC 2 cut(s) 15, 248
MboI GATC 2 cut(s) 13, 246
MboII GAAGA 2 cut(s) 241, 356
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 4 cut(s) 271, 292, 450, 467
MlyI GAGTC 2 cut(s) 91, 308
MnlI CCTC 3 cut(s) 114, 235, 522
MroNI GCCGGC 1 cut(s) 120
MroXI GAANNNNTTC 1 cut(s) 42
MseI TTAA 1 cut(s) 470
MspI CCGG 3 cut(s) 121, 228, 289
MspR9I CCNGG 1 cut(s) 193
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 396
NaeI GCCGGC 1 cut(s) 122
NdeII GATC 2 cut(s) 13, 246
NgoMIV GCCGGC 1 cut(s) 120
NlaIII CATG 4 cut(s) 70, 81, 412, 421
NlaIV GGNNCC 5 cut(s) 7, 45, 185, 385, 533
NmuCI GTSAC 1 cut(s) 61
NspI RCATGY 1 cut(s) 412
PdiI GCCGGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 42
PflFI GACNNNGTC 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 1 cut(s) 516
PleI GAGTC 2 cut(s) 90, 307
PpsI GAGTC 2 cut(s) 90, 307
PpuMI RGGWCCY 1 cut(s) 184
Psp124BI GAGCTC 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 191
PspFI CCCAGC 2 cut(s) 142, 211
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 5 cut(s) 7, 45, 185, 385, 533
PspPI GGNCC 2 cut(s) 184, 384
PspPPI RGGWCCY 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 330
PsuI RGATCY 1 cut(s) 246
PsyI GACNNNGTC 1 cut(s) 182
RigI GGCCGGCC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 1 cut(s) 515
Sau3AI GATC 2 cut(s) 13, 246
Sau96I GGNCC 2 cut(s) 184, 384
SchI GAGTC 2 cut(s) 91, 308
ScrFI CCNGG 1 cut(s) 193
SduI GDGCHC 1 cut(s) 312
SetI ASST 7 cut(s) 74, 227, 312, 329, 359, 445, 483
SfaNI GCATC 1 cut(s) 501
SinI GGWCC 1 cut(s) 184
Sse9I AATT 4 cut(s) 271, 292, 450, 467
SspMI CTAG 3 cut(s) 29, 150, 188
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 191
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 437
TaiI ACGT 1 cut(s) 74
TaqI TCGA 1 cut(s) 110
TasI AATT 4 cut(s) 271, 292, 450, 467
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 1 cut(s) 61
Tth111I GACNNNGTC 1 cut(s) 182
Van91I CCANNNNNTGG 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 3 cut(s) 29, 150, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.