MD07G1153000.v1.1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015426
Physical Location & Seq
Forward (+)
22294319 .. 22297203
2885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1153000.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGACAAACGTTTTCTCCACTTGGTTTACACAATGGCTAAGCTTGTTGTTAATTTTTGCTTACAATTTTCATGTAATGGCACAATGGCCTAGTGGAGGATCAACCAGGTTCTATGATTTCAAGGTACAAACCAAAAGAGTTACTAGACTGTGCAACACCAAGGGCATCGTGACCATCAATGGAATGTTCCCGGGGCCATCTGTATATGCTCAAGAAGATGACAGAATCGTAGTCAAAGTAACCAATGAGACACCTTACAATACCACAATTCACTGGCACGGCGTCAGGCAGAAGCTATCGTGCTGGTTTGATGGACCTTCATACATAACCCAGTGTCCAATTCAAGCTGGCCGGAGTTTTACATATGAGTTTACAATGGTGAAGCAGAAGGGCACCTTTTTTTGGCATGCTCATGTTTCATGGCTTAGGGCAACTGTTTACGGTGCCCTTTTGGTGTACCCAAAAACTGGCGTTCCCTACCCATTTAAAGTTCCTCATGAAGAGCATACCTTAATTCTAGGGGAGTATTGGATGCAGGATGTTGTGGAACTTGAGCTTAACACTACCGCAAGTGGAGGGCCTCCTACACCTGCAAATGCATATACAATCAATGGCCAACCAGGCCCCAACTACAACTGCTCCAACAATGATGTATACCACATTGATGTGGTATCAGGGAAGACATACTTGCTAAGGCTAATACATGCAGGTTTAAACATGGAGAACTTCTTTGCCATAGCCAATCACAATCTGACAATTGTCGAAGCGGATGGAGAGTACACAAAACCATTCACTACGGACCGTGTGATGCTTGGGCCAGGCCAAACAATGAGCGTTCTGGTCACCACTGACCAATCTCTAGGAAAATATTCCATGGCAATGGGACCTTACATGTCCGCTAAGGGAGTCAAATTCCAAAACATTTCATCCATTGCTTACTTCCAGTACTTAGGTGCTGTCCCTAATACCTTATCTTTGCCTGCTAAACTACCCGTCTTCAATGATAATCTTGCCGTTAAGACGGTTATGGACGGATTAAGGAGCCTAAACAATTGTGTTAATGTGCCAAAACAAATTGACACTGAACTCTTTGTCACCATTGGATTGAATGTTCAGAAGTGTCGATCCAAGACACCCGAAAAAAATTGTCGAGGCCTTAATAATGGGGTTATGGCAGCTTCGATGAATAATATAAGCTTCGTTAAGCCAAAAACGTCACTTTTGGAAGCGTATTATAAGAAGATTAATGGCTCTTTCACTGAGGATTTTCCTGGGGTACCCCTCAAGTACTATGACTTTGTCAATGGGGCGCCTAATAACGTTCCTAATAATACACAAGCTCTGAATGGGACTAGGGCATTGGTCCTTGAATATGGTACTAGAGTGCAACTCATCATGCAGGATACTGGGACAGTCACTACTGAGAACCACCCAATTCATCTTCACGGCTATAGCTTCTATGTTGTTGGGTATGGGAGTGGGAATTTCGATCTGAAAACTGCAAACTTCAACCTGGTTGACCCGCCTTATATGAACACAATCGGAGTCCCAGTGGGCGGATGGGCTGCTATACGTTTCGTTGCCGATAATCCTGGGGTTTGGTTTATGCATTGTCACCTTGACATACATCAGTCTTGGGGTTTAGGGACGGTACTTATCGTGACTAATGGTCAAGGAGAGCTTGAGACCCTTCCTCATCCTCCTGCAGACTTGCCTCAGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

575

Amino Acids

63.95

Weight (kDa)

7.62

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 42 - 155 1.1e-40 Multicopper oxidase
Cu-oxidase PF00394 167 - 318 8.5e-40 Multicopper oxidase
Cu-oxidase_2 PF07731 439 - 557 1.4e-38 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46570 AT2G46570
fragaria_vesca FvH4_7g16960 FvH4_7g16960 FvH4_7g16960
malus_domestica MD07G1153000.v1.1
prunus_persica Prupe.2G191900_v2.0.a1
pyrus_communis pycom07g15100
rosa_chinensis RchiOBHm_Chr1g0360211
rosa_laevigata RLG00000027820
rosa_multiflora Rmu_ssc0000368.1_g000050
rosa_roxburghii Rroxscaffold_4G00296290
rosa_rugosa Rorug01G0276000
rosa_samantha Rh1BG255300 Rh1CG272600
rosa_wichuraiana Rw1G025700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1236
AarI CACCTGC 1 cut(s) 598
Acc36I ACCTGC 2 cut(s) 598, 698
Acc65I GGTACC 1 cut(s) 1276
AccB1I GGYRCC 4 cut(s) 392, 443, 1276, 1309
AccB7I CCANNNNNTGG 1 cut(s) 467
AccI GTMKAC 1 cut(s) 654
AciI CCGC 5 cut(s) 567, 767, 897, 1523, 1557
AclI AACGTT 2 cut(s) 9, 1320
AclWI GGATC 2 cut(s) 106, 1119
AcoI YGGCCR 2 cut(s) 349, 613
AcsI RAATTY 2 cut(s) 911, 1483
AcyI GRCGYC 2 cut(s) 282, 1310
AfaI GTAC 8 cut(s) 126, 458, 779, 947, 1278, 1289, 1378, 1653
AfiI CCNNNNNNNGG 4 cut(s) 95, 402, 467, 1556
AflIII ACRYGT 1 cut(s) 891
AgsI TTSAA 6 cut(s) 121, 344, 1000, 1108, 1370, 1510
AhdI GACNNNNNGTC 1 cut(s) 1668
AjnI CCWGG 6 cut(s) 104, 619, 817, 1270, 1512, 1591
AloI GAACNNNNNNTCC 2 cut(s) 1095, 1127
AluBI AGCT 9 cut(s) 42, 295, 347, 556, 1178, 1197, 1340, 1455, 1681
AluI AGCT 9 cut(s) 42, 295, 347, 556, 1178, 1197, 1340, 1455, 1681
Alw26I GTCTC 2 cut(s) 242, 1679
AlwI GGATC 2 cut(s) 106, 1119
Ama87I CYCGRG 1 cut(s) 190
AoxI GGCC 9 cut(s) 86, 194, 349, 578, 613, 622, 815, 820, 1153
ApeKI GCWGC 2 cut(s) 1175, 1565
ApoI RAATTY 2 cut(s) 911, 1483
AseI ATTAAT 1 cut(s) 1245
Asp700I GAANNNNTTC 1 cut(s) 868
Asp718I GGTACC 1 cut(s) 1276
AspLEI GCGC 1 cut(s) 1312
AspS9I GGNCC 8 cut(s) 194, 314, 578, 623, 799, 815, 884, 1363
AsuC2I CCSGG 2 cut(s) 191, 192
AsuHPI GGTGA 4 cut(s) 391, 835, 1087, 1607
AvaI CYCGRG 1 cut(s) 190
AvaII GGWCC 4 cut(s) 314, 799, 884, 1363
BaeGI GKGCMC 2 cut(s) 395, 448
BaeI ACNNNNGTAYC 2 cut(s) 1368, 1401
BalI TGGCCA 1 cut(s) 615
BanI GGYRCC 4 cut(s) 392, 443, 1276, 1309
BbsI GAAGAC 2 cut(s) 686, 988
BbvI GCAGC 2 cut(s) 1187, 1552
BccI CCATC 5 cut(s) 182, 205, 305, 764, 1554
BceAI ACGGC 3 cut(s) 295, 998, 1462
BciT130I CCWGG 6 cut(s) 106, 621, 819, 1272, 1514, 1593
BciVI GTATCC 1 cut(s) 1396
BcnI CCSGG 2 cut(s) 191, 192
BcoDI GTCTC 2 cut(s) 242, 1679
BfaI CTAG 7 cut(s) 90, 144, 518, 860, 1353, 1380, 1723
BfmI CTRYAG 2 cut(s) 1450, 1704
BfoI RGCGCY 1 cut(s) 1313
BfuAI ACCTGC 2 cut(s) 598, 698
BfuI GTATCC 1 cut(s) 1396
BglI GCCNNNNNGGC 1 cut(s) 621
BisI GCNGC 2 cut(s) 1176, 1566
BlpI GCTNAGC 1 cut(s) 38
BlsI GCNGC 2 cut(s) 1177, 1567
BmcAI AGTACT 2 cut(s) 947, 1289
Bme1390I CCNGG 8 cut(s) 106, 191, 192, 621, 819, 1272, 1514, 1593
Bme18I GGWCC 4 cut(s) 314, 799, 884, 1363
BmeRI GACNNNNNGTC 1 cut(s) 1668
BmeT110I CYCGRG 1 cut(s) 190
BmgT120I GGNCC 8 cut(s) 194, 314, 578, 623, 799, 815, 884, 1363
BmiI GGNNCC 8 cut(s) 195, 394, 445, 625, 885, 1043, 1278, 1311
BmrFI CCNGG 8 cut(s) 106, 191, 192, 621, 819, 1272, 1514, 1593
BmrI ACTGGG 3 cut(s) 325, 1416, 1544
BmsI GCATC 3 cut(s) 174, 522, 798
BmuI ACTGGG 3 cut(s) 325, 1416, 1544
BoxI GACNNNNGTC 1 cut(s) 758
BpiI GAAGAC 2 cut(s) 686, 988
BplI GAGNNNNNCTC 2 cut(s) 1374, 1406
Bpu10I CCTNAGC 3 cut(s) 425, 692, 900
Bpu1102I GCTNAGC 1 cut(s) 38
BpuEI CTTGAG 4 cut(s) 195, 572, 1268, 1703
BpuMI CCSGG 2 cut(s) 191, 192
BsaHI GRCGYC 2 cut(s) 282, 1310
BsaI GGTCTC 1 cut(s) 1679
BsaJI CCNNGG 6 cut(s) 159, 190, 191, 873, 1271, 1592
BsaXI ACNNNNNCTCC 5 cut(s) 29, 623, 653, 1536, 1566
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 402, 467, 1556
Bse1I ACTGG 6 cut(s) 278, 331, 472, 943, 1411, 1550
Bse3DI GCAATG 2 cut(s) 885, 930
BseBI CCWGG 6 cut(s) 106, 621, 819, 1272, 1514, 1593
BseDI CCNNGG 6 cut(s) 159, 190, 191, 873, 1271, 1592
BseGI GGATG 6 cut(s) 537, 544, 775, 926, 1565, 1696
BseLI CCNNNNNNNGG 4 cut(s) 95, 402, 467, 1556
BseMI GCAATG 2 cut(s) 885, 930
BseMII CTCAG 2 cut(s) 1251, 1413
BseNI ACTGG 6 cut(s) 278, 331, 472, 943, 1411, 1550
BseSI GKGCMC 2 cut(s) 395, 448
BseXI GCAGC 2 cut(s) 1187, 1552
BshFI GGCC 9 cut(s) 88, 196, 351, 580, 615, 624, 817, 822, 1155
BshNI GGYRCC 4 cut(s) 392, 443, 1276, 1309
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 2 cut(s) 191, 352
BslFI GGGAC 6 cut(s) 897, 944, 1363, 1423, 1532, 1660
BslI CCNNNNNNNGG 4 cut(s) 95, 402, 467, 1556
BsmAI GTCTC 2 cut(s) 242, 1679
BsmFI GGGAC 6 cut(s) 897, 944, 1363, 1423, 1532, 1660
BsnI GGCC 9 cut(s) 88, 196, 351, 580, 615, 624, 817, 822, 1155
Bso31I GGTCTC 1 cut(s) 1679
BsoBI CYCGRG 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 395, 448
Bsp143I GATC 3 cut(s) 98, 1124, 1489
Bsp1720I GCTNAGC 1 cut(s) 38
Bsp19I CCATGG 1 cut(s) 873
BspACI CCGC 5 cut(s) 567, 767, 897, 1523, 1557
BspANI GGCC 9 cut(s) 88, 196, 351, 580, 615, 624, 817, 822, 1155
BspCNI CTCAG 2 cut(s) 1252, 1414
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 8 cut(s) 195, 394, 445, 625, 885, 1043, 1278, 1311
BspMAI CTGCAG 1 cut(s) 1708
BspMI ACCTGC 2 cut(s) 598, 698
BspPI GGATC 2 cut(s) 106, 1119
BspQI GCTCTTC 1 cut(s) 495
BspT107I GGYRCC 4 cut(s) 392, 443, 1276, 1309
BspTNI GGTCTC 1 cut(s) 1679
BsrDI GCAATG 2 cut(s) 885, 930
BsrI ACTGG 6 cut(s) 278, 331, 472, 943, 1411, 1550
BssECI CCNNGG 6 cut(s) 159, 190, 191, 873, 1271, 1592
BssMI GATC 3 cut(s) 98, 1124, 1489
BssNAI GTATAC 1 cut(s) 655
BssNI GRCGYC 2 cut(s) 282, 1310
BssT1I CCWWGG 2 cut(s) 159, 873
Bst1107I GTATAC 1 cut(s) 655
Bst2UI CCWGG 6 cut(s) 106, 621, 819, 1272, 1514, 1593
Bst4CI ACNGT 7 cut(s) 150, 436, 443, 803, 1024, 1414, 1651
Bst6I CTCTTC 1 cut(s) 495
BstACI GRCGYC 2 cut(s) 282, 1310
BstC8I GCNNGC 3 cut(s) 349, 408, 981
BstDEI CTNAG 8 cut(s) 38, 425, 692, 900, 949, 1260, 1422, 1716
BstDSI CCRYGG 1 cut(s) 873
BstEII GGTNACC 1 cut(s) 841
BstENI CCTNNNNNAGG 1 cut(s) 93
BstF5I GGATG 6 cut(s) 537, 544, 775, 926, 1565, 1696
BstH2I RGCGCY 1 cut(s) 1313
BstHHI GCGC 1 cut(s) 1312
BstKTI GATC 3 cut(s) 101, 1127, 1492
BstMAI GTCTC 2 cut(s) 242, 1679
BstMBI GATC 3 cut(s) 98, 1124, 1489
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstNI CCWGG 6 cut(s) 106, 621, 819, 1272, 1514, 1593
BstNSI RCATGY 3 cut(s) 410, 707, 895
BstPAI GACNNNNGTC 1 cut(s) 758
BstPI GGTNACC 1 cut(s) 841
BstSCI CCNGG 8 cut(s) 104, 189, 190, 619, 817, 1270, 1512, 1591
BstSFI CTRYAG 2 cut(s) 1450, 1704
BstSLI GKGCMC 2 cut(s) 395, 448
BstV1I GCAGC 2 cut(s) 1187, 1552
BstV2I GAAGAC 2 cut(s) 686, 988
BstXI CCANNNNNNTGG 1 cut(s) 880
BstZ17I GTATAC 1 cut(s) 655
BsuI GTATCC 1 cut(s) 1396
BsuRI GGCC 9 cut(s) 88, 196, 351, 580, 615, 624, 817, 822, 1155
BtgI CCRYGG 1 cut(s) 873
BtsCI GGATG 6 cut(s) 537, 544, 775, 926, 1565, 1696
BtsIMutI CAGTG 7 cut(s) 271, 338, 846, 1080, 1257, 1557, 1724
BveI ACCTGC 2 cut(s) 598, 698
Cac8I GCNNGC 3 cut(s) 349, 408, 981
CciI TCATGA 1 cut(s) 496
CfoI GCGC 1 cut(s) 1312
Cfr13I GGNCC 8 cut(s) 194, 314, 578, 623, 799, 815, 884, 1363
Cfr9I CCCGGG 1 cut(s) 190
CpoI CGGWCCG 1 cut(s) 799
CseI GACGC 1 cut(s) 271
CsiI ACCWGGT 2 cut(s) 104, 1512
Csp6I GTAC 8 cut(s) 125, 457, 778, 946, 1277, 1288, 1377, 1652
CspI CGGWCCG 1 cut(s) 799
CviQI GTAC 8 cut(s) 125, 457, 778, 946, 1277, 1288, 1377, 1652
DdeI CTNAG 8 cut(s) 38, 425, 692, 900, 949, 1260, 1422, 1716
DinI GGCGCC 1 cut(s) 1311
DpnI GATC 3 cut(s) 100, 1126, 1491
DpnII GATC 3 cut(s) 98, 1124, 1489
DraI TTTAAA 2 cut(s) 487, 714
DriI GACNNNNNGTC 1 cut(s) 1668
EaeI YGGCCR 2 cut(s) 349, 613
Eam1104I CTCTTC 1 cut(s) 495
Eam1105I GACNNNNNGTC 1 cut(s) 1668
EarI CTCTTC 1 cut(s) 495
EciI GGCGGA 1 cut(s) 1572
Eco130I CCWWGG 2 cut(s) 159, 873
Eco147I AGGCCT 1 cut(s) 1155
Eco31I GGTCTC 1 cut(s) 1679
Eco47I GGWCC 4 cut(s) 314, 799, 884, 1363
Eco88I CYCGRG 1 cut(s) 190
Eco91I GGTNACC 1 cut(s) 841
EcoNI CCTNNNNNAGG 1 cut(s) 93
EcoO109I RGGNCCY 3 cut(s) 578, 623, 884
EcoO65I GGTNACC 1 cut(s) 841
EcoRII CCWGG 6 cut(s) 104, 619, 817, 1270, 1512, 1591
EcoT14I CCWWGG 2 cut(s) 159, 873
EcoT22I ATGCAT 2 cut(s) 601, 1611
EgeI GGCGCC 1 cut(s) 1311
EheI GGCGCC 1 cut(s) 1311
ErhI CCWWGG 2 cut(s) 159, 873
FalI AAGNNNNNCTT 6 cut(s) 380, 412, 671, 703, 1665, 1697
FaqI GGGAC 6 cut(s) 897, 944, 1363, 1423, 1532, 1660
FauI CCCGC 1 cut(s) 1530
FauNDI CATATG 1 cut(s) 364
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 2 cut(s) 1176, 1566
FokI GGATG 6 cut(s) 544, 551, 782, 913, 1572, 1683
Fsp4HI GCNGC 2 cut(s) 1176, 1566
FspBI CTAG 7 cut(s) 90, 144, 518, 860, 1353, 1380, 1723
GlaI GCGC 1 cut(s) 1311
GluI GCNGC 2 cut(s) 1176, 1566
HaeII RGCGCY 1 cut(s) 1313
HaeIII GGCC 9 cut(s) 88, 196, 351, 580, 615, 624, 817, 822, 1155
HapII CCGG 2 cut(s) 191, 352
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 1312
Hin1I GRCGYC 2 cut(s) 282, 1310
Hin6I GCGC 1 cut(s) 1310
HinP1I GCGC 1 cut(s) 1310
HincII GTYRAC 1 cut(s) 1519
HindII GTYRAC 1 cut(s) 1519
HindIII AAGCTT 2 cut(s) 40, 1195
HinfI GANTC 3 cut(s) 225, 906, 1545
HpaII CCGG 2 cut(s) 191, 352
HphI GGTGA 4 cut(s) 391, 835, 1087, 1607
Hpy166II GTNNAC 7 cut(s) 27, 372, 439, 457, 655, 780, 1519
Hpy188I TCNGA 5 cut(s) 753, 1116, 1344, 1494, 1544
Hpy188III TCNNGA 4 cut(s) 169, 212, 497, 1660
Hpy8I GTNNAC 7 cut(s) 27, 372, 439, 457, 655, 780, 1519
HpyAV CCTTC 3 cut(s) 327, 382, 1700
HpyCH4III ACNGT 7 cut(s) 150, 436, 443, 803, 1024, 1414, 1651
HpyCH4IV ACGT 4 cut(s) 9, 1214, 1320, 1573
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpyF3I CTNAG 8 cut(s) 38, 425, 692, 900, 949, 1260, 1422, 1716
HpySE526I ACGT 4 cut(s) 9, 1214, 1320, 1573
Hsp92I GRCGYC 2 cut(s) 282, 1310
HspAI GCGC 1 cut(s) 1310
KasI GGCGCC 1 cut(s) 1309
KpnI GGTACC 1 cut(s) 1280
Kzo9I GATC 3 cut(s) 98, 1124, 1489
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 644, 1041
Lsp1109I GCAGC 2 cut(s) 1187, 1552
LweI GCATC 3 cut(s) 174, 522, 798
MabI ACCWGGT 2 cut(s) 104, 1512
MaeI CTAG 7 cut(s) 90, 144, 518, 860, 1353, 1380, 1723
MaeII ACGT 4 cut(s) 9, 1214, 1320, 1573
MaeIII GTNAC 9 cut(s) 139, 169, 238, 841, 1093, 1215, 1414, 1613, 1660
MalI GATC 3 cut(s) 100, 1126, 1491
MboI GATC 3 cut(s) 98, 1124, 1489
MboII GAAGA 6 cut(s) 227, 512, 691, 988, 1252, 1433
MfeI CAATTG 2 cut(s) 756, 1051
MhlI GDGCHC 2 cut(s) 395, 448
MlsI TGGCCA 1 cut(s) 615
MluNI TGGCCA 1 cut(s) 615
Mly113I GGCGCC 1 cut(s) 1310
MlyI GAGTC 2 cut(s) 915, 1554
MmeI TCCRAC 1 cut(s) 666
Mox20I TGGCCA 1 cut(s) 615
Mph1103I ATGCAT 2 cut(s) 601, 1611
MroXI GAANNNNTTC 1 cut(s) 868
MscI TGGCCA 1 cut(s) 615
MslI CAYNNNNRTG 4 cut(s) 411, 663, 665, 878
Msp20I TGGCCA 1 cut(s) 615
MspI CCGG 2 cut(s) 191, 352
MspR9I CCNGG 8 cut(s) 106, 191, 192, 621, 819, 1272, 1514, 1593
MssI GTTTAAAC 1 cut(s) 714
MunI CAATTG 2 cut(s) 756, 1051
MvaI CCWGG 6 cut(s) 106, 621, 819, 1272, 1514, 1593
MwoI GCNNNNNNNGC 1 cut(s) 621
NarI GGCGCC 1 cut(s) 1310
NciI CCSGG 2 cut(s) 191, 192
NcoI CCATGG 1 cut(s) 873
NdeI CATATG 1 cut(s) 364
NdeII GATC 3 cut(s) 98, 1124, 1489
NlaIV GGNNCC 8 cut(s) 195, 394, 445, 625, 885, 1043, 1278, 1311
NmuCI GTSAC 7 cut(s) 169, 841, 1093, 1215, 1414, 1613, 1660
NsiI ATGCAT 2 cut(s) 601, 1611
NspI RCATGY 3 cut(s) 410, 707, 895
PaeI GCATGC 1 cut(s) 410
PagI TCATGA 1 cut(s) 496
PaqCI CACCTGC 1 cut(s) 598
PceI AGGCCT 1 cut(s) 1155
PciI ACATGT 1 cut(s) 891
PciSI GCTCTTC 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 868
PfeI GAWTC 1 cut(s) 225
PflFI GACNNNGTC 1 cut(s) 1298
PflMI CCANNNNNTGG 1 cut(s) 467
PkrI GCNGC 2 cut(s) 1177, 1567
PleI GAGTC 2 cut(s) 914, 1553
PluTI GGCGCC 1 cut(s) 1313
PmeI GTTTAAAC 1 cut(s) 714
PpsI GAGTC 2 cut(s) 914, 1553
PpuMI RGGWCCY 1 cut(s) 884
PscI ACATGT 1 cut(s) 891
PshAI GACNNNNGTC 1 cut(s) 758
PshBI ATTAAT 1 cut(s) 1245
PsiI TTATAA 1 cut(s) 1236
Psp1406I AACGTT 2 cut(s) 9, 1320
Psp5II RGGWCCY 1 cut(s) 884
Psp6I CCWGG 6 cut(s) 104, 619, 817, 1270, 1512, 1591
PspEI GGTNACC 1 cut(s) 841
PspGI CCWGG 6 cut(s) 104, 619, 817, 1270, 1512, 1591
PspN4I GGNNCC 8 cut(s) 195, 394, 445, 625, 885, 1043, 1278, 1311
PspPI GGNCC 8 cut(s) 194, 314, 578, 623, 799, 815, 884, 1363
PspPPI RGGWCCY 1 cut(s) 884
PstI CTGCAG 1 cut(s) 1708
PsyI GACNNNGTC 1 cut(s) 1298
RsaI GTAC 8 cut(s) 126, 458, 779, 947, 1278, 1289, 1378, 1653
RsaNI GTAC 8 cut(s) 125, 457, 778, 946, 1277, 1288, 1377, 1652
RseI CAYNNNNRTG 4 cut(s) 411, 663, 665, 878
Rsr2I CGGWCCG 1 cut(s) 799
RsrII CGGWCCG 1 cut(s) 799
SapI GCTCTTC 1 cut(s) 495
SatI GCNGC 2 cut(s) 1176, 1566
Sau3AI GATC 3 cut(s) 98, 1124, 1489
Sau96I GGNCC 8 cut(s) 194, 314, 578, 623, 799, 815, 884, 1363
ScaI AGTACT 2 cut(s) 947, 1289
SchI GAGTC 2 cut(s) 915, 1554
ScrFI CCNGG 8 cut(s) 106, 191, 192, 621, 819, 1272, 1514, 1593
SduI GDGCHC 2 cut(s) 395, 448
SexAI ACCWGGT 2 cut(s) 104, 1512
SfaNI GCATC 3 cut(s) 174, 522, 798
SfcI CTRYAG 2 cut(s) 1450, 1704
SfiI GGCCNNNNNGGCC 1 cut(s) 621
SfoI GGCGCC 1 cut(s) 1311
SinI GGWCC 4 cut(s) 314, 799, 884, 1363
SmaI CCCGGG 1 cut(s) 192
SmiMI CAYNNNNRTG 4 cut(s) 411, 663, 665, 878
SmlI CTYRAG 4 cut(s) 210, 551, 1283, 1682
SmoI CTYRAG 4 cut(s) 210, 551, 1283, 1682
SphI GCATGC 1 cut(s) 410
SseBI AGGCCT 1 cut(s) 1155
SsiI CCGC 5 cut(s) 567, 767, 897, 1523, 1557
SspDI GGCGCC 1 cut(s) 1309
SspI AATATT 1 cut(s) 869
SspMI CTAG 7 cut(s) 90, 144, 518, 860, 1353, 1380, 1723
StuI AGGCCT 1 cut(s) 1155
StyD4I CCNGG 8 cut(s) 104, 189, 190, 619, 817, 1270, 1512, 1591
StyI CCWWGG 2 cut(s) 159, 873
TaaI ACNGT 7 cut(s) 150, 436, 443, 803, 1024, 1414, 1651
TaiI ACGT 4 cut(s) 12, 1217, 1323, 1576
TaqI TCGA 5 cut(s) 762, 1123, 1150, 1181, 1488
TatI WGTACW 3 cut(s) 777, 945, 1287
TfiI GAWTC 1 cut(s) 225
TscAI CASTG 7 cut(s) 278, 338, 853, 1087, 1264, 1557, 1724
TseFI GTSAC 7 cut(s) 169, 841, 1093, 1215, 1414, 1613, 1660
TseI GCWGC 2 cut(s) 1175, 1565
Tsp45I GTSAC 7 cut(s) 169, 841, 1093, 1215, 1414, 1613, 1660
TspDTI ATGAA 8 cut(s) 59, 309, 408, 513, 915, 1199, 1427, 1547
TspGWI ACGGA 2 cut(s) 812, 1047
TspMI CCCGGG 1 cut(s) 190
TspRI CASTG 7 cut(s) 278, 338, 853, 1087, 1264, 1557, 1724
Tth111I GACNNNGTC 1 cut(s) 1298
Van91I CCANNNNNTGG 1 cut(s) 467
VpaK11BI GGWCC 4 cut(s) 314, 799, 884, 1363
VspI ATTAAT 1 cut(s) 1245
XagI CCTNNNNNAGG 1 cut(s) 93
XapI RAATTY 2 cut(s) 911, 1483
XceI RCATGY 3 cut(s) 410, 707, 895
XcmI CCANNNNNNNNNTGG 1 cut(s) 1557
XmaI CCCGGG 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 654
XmnI GAANNNNTTC 1 cut(s) 868
XspI CTAG 7 cut(s) 90, 144, 518, 860, 1353, 1380, 1723
ZrmI AGTACT 2 cut(s) 947, 1289
Zsp2I ATGCAT 2 cut(s) 601, 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.