MD07G1200800.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0028881
Physical Location & Seq
Reverse (-)
27859907 .. 27860743
837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1200800.v1.1.491

Sequence Viewer

Length: 276 bp
ATGGCTGGGGTGTCACTGAGGAGGCTGCCAACTGAGTTCCAAGAGCTGGAGGAGAGGCTAACCAGTTTAATATTTATGACTAGTGGGAGTAGTCTTTTTCCTGGCATGAGCGGGCGCTTGGACGCTGGAATCCGGCTGACTCGGCCTTGTGGTTCTCCCATAAGGATAGCCAAAGCATTGGATCCGATATTGGATGCATGGCGCGGGGCTTCCGCTTACGCTGCCGCCTCCCATTTCCCGACACAAACTTTCAGCAAGGAAGAGTATTTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

92

Amino Acids

10.03

Weight (kDa)

6.57

Isoelectric Point (pI)

51.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 15 - 91 4.9e-11 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028881)

Species Orthologous Gene IDs
malus_domestica MD07G1200800.v1.1
pyrus_communis pycom07g18790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 46
AccBSI CCGCTC 1 cut(s) 111
AccII CGCG 1 cut(s) 204
AciI CCGC 4 cut(s) 111, 204, 213, 225
AclWI GGATC 2 cut(s) 176, 189
AfiI CCNNNNNNNGG 1 cut(s) 46
AhlI ACTAGT 1 cut(s) 80
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
AlwI GGATC 2 cut(s) 176, 189
AoxI GGCC 1 cut(s) 143
ApeKI GCWGC 2 cut(s) 25, 221
AspLEI GCGC 2 cut(s) 117, 204
BamHI GGATCC 1 cut(s) 181
BbvI GCAGC 2 cut(s) 12, 208
BciT130I CCWGG 1 cut(s) 102
BcuI ACTAGT 1 cut(s) 80
BfaI CTAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 118
BisI GCNGC 3 cut(s) 26, 222, 225
BlsI GCNGC 3 cut(s) 27, 223, 226
Bme1390I CCNGG 1 cut(s) 102
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 102
BmsI GCATC 1 cut(s) 184
BpmI CTGGAG 1 cut(s) 68
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 8, 24
BseNI ACTGG 1 cut(s) 63
BseRI GAGGAG 2 cut(s) 34, 65
BseXI GCAGC 2 cut(s) 12, 208
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 1 cut(s) 145
BsiSI CCGG 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 46
BsnI GGCC 1 cut(s) 145
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 4 cut(s) 111, 204, 213, 225
BspANI GGCC 1 cut(s) 145
BspCNI CTCAG 2 cut(s) 9, 25
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 183
BspPI GGATC 2 cut(s) 176, 189
BsrBI CCGCTC 1 cut(s) 111
BsrI ACTGG 1 cut(s) 63
BssMI GATC 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 102
Bst6I CTCTTC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 113
BstDEI CTNAG 2 cut(s) 17, 33
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 117, 204
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 2 cut(s) 142, 221
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 1 cut(s) 100
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 12, 208
BstX2I RGATCY 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 178
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 1 cut(s) 145
BtsCI GGATG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 113
CfoI GCGC 2 cut(s) 117, 204
CseI GACGC 1 cut(s) 131
CviAII CATG 2 cut(s) 106, 198
CviJI RGCY 8 cut(s) 5, 25, 46, 58, 136, 145, 170, 209
CviKI_1 RGCY 8 cut(s) 5, 25, 46, 58, 136, 145, 170, 209
DdeI CTNAG 2 cut(s) 17, 33
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 199
FaeI CATG 2 cut(s) 109, 201
FaiI YATR 4 cut(s) 77, 107, 161, 199
FatI CATG 2 cut(s) 105, 197
FauI CCCGC 2 cut(s) 104, 197
Fnu4HI GCNGC 3 cut(s) 26, 222, 225
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 3 cut(s) 26, 222, 225
FspBI CTAG 1 cut(s) 81
GlaI GCGC 2 cut(s) 116, 203
GluI GCNGC 3 cut(s) 26, 222, 225
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 145
HapII CCGG 1 cut(s) 133
HgaI GACGC 1 cut(s) 131
HhaI GCGC 2 cut(s) 117, 204
Hin1II CATG 2 cut(s) 109, 201
Hin6I GCGC 2 cut(s) 115, 202
HinP1I GCGC 2 cut(s) 115, 202
HinfI GANTC 2 cut(s) 129, 139
HpaII CCGG 1 cut(s) 133
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 1 cut(s) 238
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 221
HpyF3I CTNAG 2 cut(s) 17, 33
Hsp92II CATG 2 cut(s) 109, 201
HspAI GCGC 2 cut(s) 115, 202
Kzo9I GATC 1 cut(s) 181
LpnPI CCDG 6 cut(s) 32, 76, 87, 111, 114, 146
Lsp1109I GCAGC 2 cut(s) 12, 208
LweI GCATC 1 cut(s) 184
MaeI CTAG 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 12
MalI GATC 1 cut(s) 183
MbiI CCGCTC 1 cut(s) 111
MboI GATC 1 cut(s) 181
MboII GAAGA 1 cut(s) 272
MflI RGATCY 1 cut(s) 181
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 5 cut(s) 12, 15, 43, 48, 238
Mph1103I ATGCAT 1 cut(s) 199
MseI TTAA 1 cut(s) 68
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 1 cut(s) 102
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 142, 221
NdeII GATC 1 cut(s) 181
NlaIII CATG 2 cut(s) 109, 201
NlaIV GGNNCC 1 cut(s) 183
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 12
NsiI ATGCAT 1 cut(s) 199
PfeI GAWTC 1 cut(s) 129
PflMI CCANNNNNTGG 1 cut(s) 46
PkrI GCNGC 3 cut(s) 27, 223, 226
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 183
PsuI RGATCY 1 cut(s) 181
SaqAI TTAA 1 cut(s) 68
SatI GCNGC 3 cut(s) 26, 222, 225
Sau3AI GATC 1 cut(s) 181
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 102
SetI ASST 1 cut(s) 48
SfaNI GCATC 1 cut(s) 184
SpeI ACTAGT 1 cut(s) 80
SsiI CCGC 4 cut(s) 111, 204, 213, 225
SspI AATATT 1 cut(s) 72
SspMI CTAG 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 100
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 129
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 2 cut(s) 25, 221
Tsp45I GTSAC 1 cut(s) 12
TspRI CASTG 1 cut(s) 21
Van91I CCANNNNNTGG 1 cut(s) 46
XspI CTAG 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.