MD07G1202900.v1.1

ARF guanine-nucleotide exchange factor GNOM-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0028874
Physical Location & Seq
Reverse (-)
28087533 .. 28090824
3292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1202900.v1.1.491

Sequence Viewer

Length: 3096 bp
ATGAATTGTTTCGAAATCTTATGCAGTTTGGTTTGGTTTTTTCCACTTATTCTTTCAATGGTATGCAGCATTGTTCTTAATTTGTACCATTTGCGCACTGAACTTAAACTACAGCTTGAGGCTTTCTTTTCTTGTCAGGAGGTTGCTATGGAGGCTATGTTGACTTCTGCAGGCAGAAAAATTGCATTGGAGATTGCATGGCTAACTATGCTCAATTGTGTATTAACATGCAGCAATGTCTTTGAACTTGCTAATCTGGTCTGGAATGGATCTGTTTCTGTACACCCAGTGGAAGAGAATCCGTTCTCGTTAGTGGTGACAACTACAGTGATCCTAGTAATTGGGTTCCATTTGTTCGCCGGAGGAAAGTACATAAAGAGAAGGCTAATGATTGGAGCTGATCATTTTAACCGACCCAAAGAAGGACTGAAAGGAATTCCTCCTCCAAGGAACATATCTGTTGCCCGACCCGACAAATCCTGTTGCGTGGCCTGCTTTTTCAGGTACACTGCTGGGTTGGACAAGAATCTAGTTGGGGATTTCTTGGGAAATCATGACGAGTTTTGTGTTCAGGTTCTTCATAAATTTGCCGGTACCTTTGATTTCACAGATATGAACTTGGATACTGCACTCCGACTGTTTTTGGAGACATTTCGATTGCCTGGAGAATCACAAAAGATACAGAGGGTGCTTGAAGCATTCTCAGAGAGATATTATGAGCAATCACCACTAATTCTAGCTAACAAGGATGCTGCGCTTTTGCTGTCATATTCACTCATAATGCTCAATACAGATCAACACAATGTTCAGGTAAAGAAGAAAATGACGGAGGAGGATTTCATTCGGAATAATCGGCACATCAATGGAGGCAGTGATCTTCCTCGAGAATTCCTGACAGAGCTTTACCACTCAATATGCAAGAATGAGATCCGAACAACTCCAGAACAAGGTGCTGGTTATCCTGAAATGACCCCAAGCCGGTGGATTGATTTAATGCACAAATCTAAGAAGAGTGCTCCATTTATCATGTCTGATTCCAGAGCCTACCTAGACCACGATATGTTTGCTATAATGTCTGGCCCTACCATTGCTGCTATCTCTGTGGTGTTTGATCATGCAGAACATGAAGAGATTTACCAAACTTGCATCGATGGATTTTTAGCCGTTGCAAAGATTTCAGCATGCCATCATCTTGAAGATGTTCTGGATGATCTGGTTGTATCTCTCTGTAAGTTTACCACCCTTTTAAACCCATCATCTGTTGAGGAGCCTGTGTTAGCCTTTGGTGATGACACAAAAGCAAGAATGGCAACTGTGACTCTATTCACAATTGCCAATAGGTATGGTGATTACATCCGCACAGGTTGGAGAAATATTCTGGACTGCATCTTAAGATTACACAAGCTTGGTCTTCTTCCAGCTCGTGTGGCCAGTGATGCAGCCGATGAGTTGGAGTTTTCTGCTGACACAGGGCCTGGGAAGCCTATATCAAATTCTCTCTCTTCTGTTCATGTACCTTCCATAGGCACTCCTAGGAGATCCTCTGGATTGATGGGTCGGTTTAGTCAGCTCTTATCTCTTGAGACAGAGGAGCCGAGATCGCAGCCTACTGAACAACAACTTGCTGCTCATCAACGCACCGTTCAGACAATTCAAAAGTGTCATATTGACAGCATATTTACTGAGAGCAAGTTTCTGCAGGCTGAATCCCTCTTACAGCTTGCACGCGCACTGGTTTGGGCTGCAGGACGACCCCAGAAAGGGAACAGCTCTCCTGAGGACGAGGATACTGCAGTTTTCTGTTTGGAGTTGCTGATTGCTATTACCCTAAATAATAGGGATAGGATTGATCTTCTTTGGCAGGGAGTGTATGAGCACATATCGACCATTGTTCAGTCAACTGTGATGCCTTGTGCTCTGGTAGAGAAGGCTGTTTTTGGACTTCTTCGGATTTGCCAGCGGCTGCTTCCCTATAAAGAGAACCTTGCTGATGATCTCTTGAAGTCCCTGCAGCTTGTCTTGAAGCTTGATGCTCGGGTTGCTGATGCTTACTGTGAGCAAATTACACAGGAAGTCAGTCGCCTTGTGAAAGCAAATGCCTCTCACATCAGATCCCATGGGGGGTGGCGCACAATTACATCCTTACTCTCTATCACAGCCCGGCACCCTGATGCTTCTGAGGCTGGGTTTAACGCACTGTTCTTCATTATGTCAGAAGGAACCCACTTGTTGCCGGCCAACTATGTTGTGTGTGTTGATGCTTCAAGGCAGTTTGCAGAATCTCGCGTTGGGCAGGCAGAACGATCTGTGTGCGCACTTGATCTTATGGCAGGTTCTGTTGATTGTTTAGCAAGGTGGGCCTGTGAGGCTAAGCAAGCTGTGAACGAGGAGGAAGCGGTGAAAATGTCTGAGGATATTGGGGAAATGTGGTTCCGACTTGTTCAGGCACTAAGAAAAGTTTGTTTGGACCAGAGAGAAGATGTCAGGAACCATGCTTTGTCGTTGTTGCAGAAGTGCTTGACGGGAGTGGATGGGATCCCTCTTCTACATGGTCTCTGGCTACAGTGCTTTGATGTGGTGATCTTCACAATGCTTGACGACTTGCTTGAAATTGCACAGGGACACTCCCAAAAGGACTACCGCAACATGGAAGGCACTCTTATCCTTGCAATGGAGCTCTTGTCCAAGGTGTTCTTACAGCTACTTCCTGATCTATCGCAGTTAACAACCTTCTGCAAACTATGGCTGGGTGTTCTCAGTCGAATGGAAAAGTATATGAAGGTGAAAGTTAGAGGGAAAAAAAGTGAGAAGCTTCAGGACCAAGTACCCAAACTACTTAAGAACACATTGCTTGTGATGAATTTAAAGGGAGTGCTCGTGCAAAGGAGCGCTCTAGGAGGTGACAGCTTGTGGGAGTTGACATGGCTACATGTCAATAACATTGCTCCATCCTTGCAATCTGAGGTTTTCCCTGATCAAGGTTCAGAGCAGCCCGAGACTAAGCTGGGTGAAAACAGAGGTCTAGTTTCGGATGAAAAGGGTAATGTACTTCCAACTGAAATGGCGTCGCCAGAAGTTTCTGGCACTGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000281 GO:0000578 GO:0000902 GO:0000910 GO:0000911 GO:0001736 GO:0001738 GO:0002009 GO:0003006 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006897 GO:0007049 GO:0007155 GO:0007164 GO:0007275 GO:0007389 GO:0008150 GO:0009605 GO:0009606 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009826 GO:0009880 GO:0009888 GO:0009914 GO:0009926 GO:0009942 GO:0009987 GO:0010008 GO:0010015 GO:0010053 GO:0010054 GO:0010087 GO:0010101 GO:0010102 GO:0010154 GO:0010274 GO:0010311 GO:0010540 GO:0010817 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016197 GO:0019899 GO:0021700 GO:0022402 GO:0022414 GO:0022610 GO:0022622 GO:0030154 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032506 GO:0032509 GO:0032879 GO:0032989 GO:0040007 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046907 GO:0046983 GO:0048209 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051049 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051649 GO:0060341 GO:0060429 GO:0060560 GO:0060627 GO:0060918 GO:0061458 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0071944 GO:0071985 GO:0090558 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098657 GO:0098772 GO:0098791 GO:0098805 GO:0099402 GO:1902410 GO:1903047 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Sec7 Sec7_N

Protein Analysis

1032

Amino Acids

115.42

Weight (kDa)

5.79

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7 PF01369 126 - 310 1.7e-63 Sec7 domain
TPR_28 PF23325 809 - 962 5.5e-14 GBF1-like, tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028874)

Species Orthologous Gene IDs
malus_domestica MD07G1202900.v1.1
rosa_multiflora Rmu_sc0006033.1_g000002

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 95, 2314
Acc36I ACCTGC 1 cut(s) 2321
Acc65I GGTACC 1 cut(s) 593
AccB1I GGYRCC 2 cut(s) 593, 2163
AccII CGCG 2 cut(s) 1728, 2286
AciI CCGC 4 cut(s) 1357, 1960, 2396, 2641
AclWI GGATC 7 cut(s) 277, 325, 922, 1533, 2106, 2530, 2543
AcoI YGGCCR 2 cut(s) 1428, 2235
AcsI RAATTY 5 cut(s) 435, 584, 887, 1492, 2860
AcuI CTGAAG 1 cut(s) 2798
AcyI GRCGYC 1 cut(s) 3065
AdeI CACNNNGTG 1 cut(s) 289
AfaI GTAC 8 cut(s) 86, 282, 371, 506, 595, 1515, 2826, 3048
AfeI AGCGCT 1 cut(s) 2890
AflII CTTAAG 2 cut(s) 1390, 2837
AflIII ACRYGT 1 cut(s) 2929
AgsI TTSAA 9 cut(s) 57, 245, 695, 1196, 1655, 2002, 2023, 2265, 2609
AjnI CCWGG 2 cut(s) 661, 1474
AjuI GAANNNNNNNTTGG 2 cut(s) 2271, 2303
AloI GAACNNNNNNTCC 2 cut(s) 2414, 2446
Alw21I GWGCWC 5 cut(s) 1018, 1878, 1918, 2679, 2877
Alw26I GTCTC 4 cut(s) 641, 1577, 2558, 2990
AlwI GGATC 7 cut(s) 277, 325, 922, 1533, 2106, 2530, 2543
AlwNI CAGNNNCTG 3 cut(s) 1475, 1963, 2336
Ama87I CYCGRG 3 cut(s) 882, 2034, 2993
Aor51HI AGCGCT 1 cut(s) 2890
AoxI GGCC 6 cut(s) 489, 1078, 1428, 1472, 2235, 2358
ApoI RAATTY 5 cut(s) 435, 584, 887, 1492, 2860
Asp700I GAANNNNTTC 3 cut(s) 8, 302, 1200
Asp718I GGTACC 1 cut(s) 593
AspA2I CCTAGG 1 cut(s) 1532
AspLEI GCGC 6 cut(s) 96, 757, 1730, 2130, 2315, 2891
AspS9I GGNCC 5 cut(s) 1079, 1472, 2358, 2467, 2818
AsuC2I CCSGG 1 cut(s) 2161
AsuHPI GGTGA 9 cut(s) 328, 717, 1298, 1358, 2410, 2590, 2794, 2912, 3020
AsuII TTCGAA 1 cut(s) 12
AvaI CYCGRG 3 cut(s) 882, 2034, 2993
AvaII GGWCC 2 cut(s) 2467, 2818
AvrII CCTAGG 1 cut(s) 1532
AxyI CCTNAGG 1 cut(s) 1776
BaeI ACNNNNGTAYC 2 cut(s) 1779, 1812
BalI TGGCCA 1 cut(s) 1430
BamHI GGATCC 1 cut(s) 2535
BanI GGYRCC 2 cut(s) 593, 2163
BanII GRGCYC 1 cut(s) 2679
BauI CACGAG 2 cut(s) 1422, 2876
BbsI GAAGAC 1 cut(s) 1403
Bbv12I GWGCWC 5 cut(s) 1018, 1878, 1918, 2679, 2877
BccI CCATC 6 cut(s) 1145, 1194, 1261, 1546, 2525, 2956
BceAI ACGGC 1 cut(s) 1148
BcgI CGANNNNNNTGC 6 cut(s) 1046, 1080, 1772, 1806, 2292, 2326
BciT130I CCWGG 2 cut(s) 663, 1476
BciVI GTATCC 2 cut(s) 616, 1780
BclI TGATCA 3 cut(s) 400, 1111, 2974
BcnI CCSGG 1 cut(s) 2161
BcoDI GTCTC 4 cut(s) 641, 1577, 2558, 2990
BfaI CTAG 7 cut(s) 335, 530, 737, 1049, 1533, 2894, 3023
BfmI CTRYAG 8 cut(s) 110, 168, 324, 1697, 1743, 1791, 2009, 2561
BfoI RGCGCY 1 cut(s) 2892
BfrI CTTAAG 2 cut(s) 1390, 2837
BfuAI ACCTGC 1 cut(s) 2321
BfuI GTATCC 2 cut(s) 616, 1780
BglI GCCNNNNNGGC 1 cut(s) 2366
BlnI CCTAGG 1 cut(s) 1532
BlpI GCTNAGC 1 cut(s) 2370
Bme1390I CCNGG 3 cut(s) 663, 1476, 2161
Bme18I GGWCC 2 cut(s) 2467, 2818
BmeT110I CYCGRG 3 cut(s) 882, 2034, 2993
BmgT120I GGNCC 5 cut(s) 1079, 1472, 2358, 2467, 2818
BmiI GGNNCC 9 cut(s) 347, 595, 1269, 1593, 2165, 2221, 2432, 2489, 2537
BmrFI CCNGG 3 cut(s) 663, 1476, 2161
BmrI ACTGGG 1 cut(s) 281
BmsI GCATC 9 cut(s) 739, 1155, 1395, 1426, 1896, 2020, 2035, 2161, 2248
BmuI ACTGGG 1 cut(s) 281
BpiI GAAGAC 1 cut(s) 1403
BpmI CTGGAG 2 cut(s) 684, 924
Bpu1102I GCTNAGC 1 cut(s) 2370
Bpu14I TTCGAA 1 cut(s) 12
BpuEI CTTGAG 2 cut(s) 137, 1601
BpuMI CCSGG 1 cut(s) 2161
Bsa29I ATCGAT 1 cut(s) 1149
BsaHI GRCGYC 1 cut(s) 3065
BsaI GGTCTC 1 cut(s) 2558
BsaJI CCNNGG 5 cut(s) 446, 1475, 1532, 2116, 2685
BsaXI ACNNNNNCTCC 8 cut(s) 143, 173, 1259, 1289, 2666, 2696, 2862, 2892
Bse118I RCCGGY 3 cut(s) 590, 978, 2233
Bse1I ACTGG 3 cut(s) 287, 1431, 1737
Bse21I CCTNAGG 1 cut(s) 1776
Bse3DI GCAATG 5 cut(s) 241, 1086, 2676, 2846, 2940
BseBI CCWGG 2 cut(s) 663, 1476
BseCI ATCGAT 1 cut(s) 1149
BseDI CCNNGG 5 cut(s) 446, 1475, 1532, 2116, 2685
BseGI GGATG 7 cut(s) 754, 1213, 1353, 2138, 2536, 2948, 3037
BseMI GCAATG 5 cut(s) 241, 1086, 2676, 2846, 2940
BseMII CTCAG 7 cut(s) 717, 1674, 1767, 2169, 2400, 2770, 2952
BseNI ACTGG 3 cut(s) 287, 1431, 1737
BseRI GAGGAG 5 cut(s) 432, 845, 1280, 1604, 2402
BseYI CCCAGC 4 cut(s) 512, 2183, 2746, 3004
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 2 cut(s) 1728, 2286
BshFI GGCC 6 cut(s) 491, 1080, 1430, 1474, 2237, 2360
BshNI GGYRCC 2 cut(s) 593, 2163
BshVI ATCGAT 1 cut(s) 1149
BsiHKAI GWGCWC 5 cut(s) 1018, 1878, 1918, 2679, 2877
BsiHKCI CYCGRG 3 cut(s) 882, 2034, 2993
BsiSI CCGG 5 cut(s) 360, 591, 979, 2161, 2234
BslFI GGGAC 2 cut(s) 1990, 2634
BsmAI GTCTC 4 cut(s) 641, 1577, 2558, 2990
BsmFI GGGAC 2 cut(s) 1990, 2634
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 6 cut(s) 491, 1080, 1430, 1474, 2237, 2360
Bso31I GGTCTC 1 cut(s) 2558
BsoBI CYCGRG 3 cut(s) 882, 2034, 2993
Bsp119I TTCGAA 1 cut(s) 12
Bsp1286I GDGCHC 5 cut(s) 1018, 1878, 1918, 2679, 2877
Bsp1407I TGTACA 1 cut(s) 280
Bsp1720I GCTNAGC 1 cut(s) 2370
Bsp19I CCATGG 1 cut(s) 2116
BspACI CCGC 4 cut(s) 1357, 1960, 2396, 2641
BspANI GGCC 6 cut(s) 491, 1080, 1430, 1474, 2237, 2360
BspCNI CTCAG 7 cut(s) 716, 1675, 1768, 2170, 2401, 2769, 2953
BspDI ATCGAT 1 cut(s) 1149
BspFNI CGCG 2 cut(s) 1728, 2286
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 9 cut(s) 347, 595, 1269, 1593, 2165, 2221, 2432, 2489, 2537
BspMAI CTGCAG 5 cut(s) 172, 1701, 1747, 1795, 2013
BspMI ACCTGC 1 cut(s) 2321
BspPI GGATC 7 cut(s) 277, 325, 922, 1533, 2106, 2530, 2543
BspT104I TTCGAA 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 593, 2163
BspTI CTTAAG 2 cut(s) 1390, 2837
BspTNI GGTCTC 1 cut(s) 2558
BsrDI GCAATG 5 cut(s) 241, 1086, 2676, 2846, 2940
BsrFI RCCGGY 3 cut(s) 590, 978, 2233
BsrGI TGTACA 1 cut(s) 280
BsrI ACTGG 3 cut(s) 287, 1431, 1737
BssAI RCCGGY 3 cut(s) 590, 978, 2233
BssECI CCNNGG 5 cut(s) 446, 1475, 1532, 2116, 2685
BssNI GRCGYC 1 cut(s) 3065
BssSI CACGAG 2 cut(s) 1422, 2876
BssT1I CCWWGG 4 cut(s) 446, 1532, 2116, 2685
Bst2BI CACGAG 2 cut(s) 1422, 2876
Bst2UI CCWGG 2 cut(s) 663, 1476
Bst4CI ACNGT 8 cut(s) 328, 639, 1315, 1642, 1903, 2054, 2199, 2565
Bst6I CTCTTC 5 cut(s) 288, 1004, 1122, 1507, 2547
BstACI GRCGYC 1 cut(s) 3065
BstAFI CTTAAG 2 cut(s) 1390, 2837
BstAUI TGTACA 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 12
BstDSI CCRYGG 1 cut(s) 2116
BstENI CCTNNNNNAGG 2 cut(s) 1521, 2976
BstF5I GGATG 7 cut(s) 754, 1213, 1353, 2138, 2536, 2948, 3037
BstFNI CGCG 2 cut(s) 1728, 2286
BstH2I RGCGCY 1 cut(s) 2892
BstHHI GCGC 6 cut(s) 96, 757, 1730, 2130, 2315, 2891
BstMAI GTCTC 4 cut(s) 641, 1577, 2558, 2990
BstNI CCWGG 2 cut(s) 663, 1476
BstNSI RCATGY 3 cut(s) 231, 1185, 2933
BstSCI CCNGG 3 cut(s) 661, 1474, 2159
BstSFI CTRYAG 8 cut(s) 110, 168, 324, 1697, 1743, 1791, 2009, 2561
BstUI CGCG 2 cut(s) 1728, 2286
BstV2I GAAGAC 1 cut(s) 1403
BstX2I RGATCY 5 cut(s) 269, 927, 1538, 2111, 2535
BstXI CCANNNNNNTGG 1 cut(s) 981
BstYI RGATCY 5 cut(s) 269, 927, 1538, 2111, 2535
Bsu15I ATCGAT 1 cut(s) 1149
Bsu36I CCTNAGG 1 cut(s) 1776
BsuI GTATCC 2 cut(s) 616, 1780
BsuRI GGCC 6 cut(s) 491, 1080, 1430, 1474, 2237, 2360
BsuTUI ATCGAT 1 cut(s) 1149
BtgI CCRYGG 1 cut(s) 2116
BtsCI GGATG 7 cut(s) 754, 1213, 1353, 2138, 2536, 2948, 3037
BtsI GCAGTG 3 cut(s) 507, 877, 3084
BveI ACCTGC 1 cut(s) 2321
CaiI CAGNNNCTG 3 cut(s) 1475, 1963, 2336
CciI TCATGA 1 cut(s) 553
CfoI GCGC 6 cut(s) 96, 757, 1730, 2130, 2315, 2891
Cfr10I RCCGGY 3 cut(s) 590, 978, 2233
Cfr13I GGNCC 5 cut(s) 1079, 1472, 2358, 2467, 2818
ClaI ATCGAT 1 cut(s) 1149
CseI GACGC 1 cut(s) 3054
Csp6I GTAC 8 cut(s) 85, 281, 370, 505, 594, 1514, 2825, 3047
CviQI GTAC 8 cut(s) 85, 281, 370, 505, 594, 1514, 2825, 3047
DraI TTTAAA 2 cut(s) 1248, 2865
DraIII CACNNNGTG 1 cut(s) 289
EaeI YGGCCR 2 cut(s) 1428, 2235
Eam1104I CTCTTC 5 cut(s) 288, 1004, 1122, 1507, 2547
EarI CTCTTC 5 cut(s) 288, 1004, 1122, 1507, 2547
Ecl136II GAGCTC 1 cut(s) 2677
Eco130I CCWWGG 4 cut(s) 446, 1532, 2116, 2685
Eco24I GRGCYC 1 cut(s) 2679
Eco31I GGTCTC 1 cut(s) 2558
Eco47I GGWCC 2 cut(s) 2467, 2818
Eco47III AGCGCT 1 cut(s) 2890
Eco53kI GAGCTC 1 cut(s) 2677
Eco57I CTGAAG 1 cut(s) 2798
Eco81I CCTNAGG 1 cut(s) 1776
Eco88I CYCGRG 3 cut(s) 882, 2034, 2993
EcoICRI GAGCTC 1 cut(s) 2677
EcoNI CCTNNNNNAGG 2 cut(s) 1521, 2976
EcoO109I RGGNCCY 1 cut(s) 1472
EcoRI GAATTC 2 cut(s) 435, 887
EcoRII CCWGG 2 cut(s) 661, 1474
EcoT14I CCWWGG 4 cut(s) 446, 1532, 2116, 2685
EcoT38I GRGCYC 1 cut(s) 2679
ErhI CCWWGG 4 cut(s) 446, 1532, 2116, 2685
FalI AAGNNNNNCTT 6 cut(s) 1968, 2000, 2643, 2675, 2678, 2710
FaqI GGGAC 2 cut(s) 1990, 2634
FbaI TGATCA 3 cut(s) 400, 1111, 2974
FokI GGATG 7 cut(s) 761, 1220, 1340, 2125, 2543, 2935, 3044
FriOI GRGCYC 1 cut(s) 2679
FspAI RTGCGCAY 1 cut(s) 2314
FspBI CTAG 7 cut(s) 335, 530, 737, 1049, 1533, 2894, 3023
FspI TGCGCA 2 cut(s) 95, 2314
GlaI GCGC 6 cut(s) 95, 756, 1729, 2129, 2314, 2890
GsaI CCCAGC 4 cut(s) 516, 2187, 2750, 3008
GsuI CTGGAG 2 cut(s) 684, 924
HaeII RGCGCY 1 cut(s) 2892
HaeIII GGCC 6 cut(s) 491, 1080, 1430, 1474, 2237, 2360
HapII CCGG 5 cut(s) 360, 591, 979, 2161, 2234
HgaI GACGC 1 cut(s) 3054
HhaI GCGC 6 cut(s) 96, 757, 1730, 2130, 2315, 2891
Hin1I GRCGYC 1 cut(s) 3065
Hin6I GCGC 6 cut(s) 94, 755, 1728, 2128, 2313, 2889
HinP1I GCGC 6 cut(s) 94, 755, 1728, 2128, 2313, 2889
HincII GTYRAC 4 cut(s) 162, 1899, 2724, 2919
HindII GTYRAC 4 cut(s) 162, 1899, 2724, 2919
HindIII AAGCTT 3 cut(s) 1403, 2024, 2810
HinfI GANTC 7 cut(s) 298, 526, 668, 1034, 1318, 1706, 2279
HpaI GTTAAC 1 cut(s) 2724
HpaII CCGG 5 cut(s) 360, 591, 979, 2161, 2234
HphI GGTGA 9 cut(s) 328, 717, 1298, 1358, 2410, 2590, 2794, 2912, 3020
Hpy166II GTNNAC 8 cut(s) 162, 283, 507, 1236, 1899, 2383, 2724, 2919
Hpy8I GTNNAC 8 cut(s) 162, 283, 507, 1236, 1899, 2383, 2724, 2919
Hpy99I CGWCG 1 cut(s) 3070
HpyAV CCTTC 8 cut(s) 375, 416, 1527, 1921, 2210, 2645, 2740, 2773
HpyCH4III ACNGT 8 cut(s) 328, 639, 1315, 1642, 1903, 2054, 2199, 2565
Hsp92I GRCGYC 1 cut(s) 3065
HspAI GCGC 6 cut(s) 94, 755, 1728, 2128, 2313, 2889
KpnI GGTACC 1 cut(s) 597
KroI GCCGGC 1 cut(s) 2233
KroNI GCCGGC 1 cut(s) 2235
Ksp22I TGATCA 3 cut(s) 400, 1111, 2974
KspAI GTTAAC 1 cut(s) 2724
LmnI GCTCC 7 cut(s) 395, 1021, 1267, 1591, 2674, 2886, 2950
LweI GCATC 9 cut(s) 739, 1155, 1395, 1426, 1896, 2020, 2035, 2161, 2248
MaeI CTAG 7 cut(s) 335, 530, 737, 1049, 1533, 2894, 3023
MaeIII GTNAC 3 cut(s) 316, 1315, 2900
MfeI CAATTG 2 cut(s) 214, 1329
MflI RGATCY 5 cut(s) 269, 927, 1538, 2111, 2535
MhlI GDGCHC 5 cut(s) 1018, 1878, 1918, 2679, 2877
MlsI TGGCCA 1 cut(s) 1430
MluNI TGGCCA 1 cut(s) 1430
MlyI GAGTC 1 cut(s) 1312
MmeI TCCRAC 6 cut(s) 498, 658, 1346, 1431, 2458, 3077
Mox20I TGGCCA 1 cut(s) 1430
MroNI GCCGGC 1 cut(s) 2233
MroXI GAANNNNTTC 3 cut(s) 8, 302, 1200
MscI TGGCCA 1 cut(s) 1430
MslI CAYNNNNRTG 3 cut(s) 611, 861, 2169
Msp20I TGGCCA 1 cut(s) 1430
MspA1I CMGCKG 1 cut(s) 1960
MspCI CTTAAG 2 cut(s) 1390, 2837
MspI CCGG 5 cut(s) 360, 591, 979, 2161, 2234
MspR9I CCNGG 3 cut(s) 663, 1476, 2161
MunI CAATTG 2 cut(s) 214, 1329
Mva1269I GAATGC 1 cut(s) 698
MvaI CCWGG 2 cut(s) 663, 1476
MvnI CGCG 2 cut(s) 1728, 2286
NaeI GCCGGC 1 cut(s) 2235
NciI CCSGG 1 cut(s) 2161
NcoI CCATGG 1 cut(s) 2116
NgoMIV GCCGGC 1 cut(s) 2233
NlaIV GGNNCC 9 cut(s) 347, 595, 1269, 1593, 2165, 2221, 2432, 2489, 2537
NmeAIII GCCGAG 1 cut(s) 1620
NmuCI GTSAC 3 cut(s) 316, 1315, 2900
NsbI TGCGCA 2 cut(s) 95, 2314
NspI RCATGY 3 cut(s) 231, 1185, 2933
NspV TTCGAA 1 cut(s) 12
PaeI GCATGC 1 cut(s) 1185
PaeR7I CTCGAG 1 cut(s) 882
PagI TCATGA 1 cut(s) 553
PciI ACATGT 1 cut(s) 2929
PctI GAATGC 1 cut(s) 698
PdiI GCCGGC 1 cut(s) 2235
PdmI GAANNNNTTC 3 cut(s) 8, 302, 1200
PfeI GAWTC 6 cut(s) 298, 526, 668, 1034, 1706, 2279
PleI GAGTC 1 cut(s) 1312
PpsI GAGTC 1 cut(s) 1312
PscI ACATGT 1 cut(s) 2929
Psp124BI GAGCTC 1 cut(s) 2679
Psp6I CCWGG 2 cut(s) 661, 1474
PspFI CCCAGC 4 cut(s) 512, 2183, 2746, 3004
PspGI CCWGG 2 cut(s) 661, 1474
PspN4I GGNNCC 9 cut(s) 347, 595, 1269, 1593, 2165, 2221, 2432, 2489, 2537
PspPI GGNCC 5 cut(s) 1079, 1472, 2358, 2467, 2818
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PstI CTGCAG 5 cut(s) 172, 1701, 1747, 1795, 2013
PstNI CAGNNNCTG 3 cut(s) 1475, 1963, 2336
PsuI RGATCY 5 cut(s) 269, 927, 1538, 2111, 2535
RsaI GTAC 8 cut(s) 86, 282, 371, 506, 595, 1515, 2826, 3048
RsaNI GTAC 8 cut(s) 85, 281, 370, 505, 594, 1514, 2825, 3047
RseI CAYNNNNRTG 3 cut(s) 611, 861, 2169
SacI GAGCTC 1 cut(s) 2679
Sau96I GGNCC 5 cut(s) 1079, 1472, 2358, 2467, 2818
SchI GAGTC 1 cut(s) 1312
ScrFI CCNGG 3 cut(s) 663, 1476, 2161
SduI GDGCHC 5 cut(s) 1018, 1878, 1918, 2679, 2877
SfaNI GCATC 9 cut(s) 739, 1155, 1395, 1426, 1896, 2020, 2035, 2161, 2248
SfcI CTRYAG 8 cut(s) 110, 168, 324, 1697, 1743, 1791, 2009, 2561
Sfr274I CTCGAG 1 cut(s) 882
SfuI TTCGAA 1 cut(s) 12
SinI GGWCC 2 cut(s) 2467, 2818
SlaI CTCGAG 1 cut(s) 882
SmiMI CAYNNNNRTG 3 cut(s) 611, 861, 2169
SmlI CTYRAG 5 cut(s) 116, 882, 1390, 1580, 2837
SmoI CTYRAG 5 cut(s) 116, 882, 1390, 1580, 2837
SphI GCATGC 1 cut(s) 1185
SsiI CCGC 4 cut(s) 1357, 1960, 2396, 2641
SspI AATATT 1 cut(s) 1375
SspMI CTAG 7 cut(s) 335, 530, 737, 1049, 1533, 2894, 3023
SstI GAGCTC 1 cut(s) 2679
StyD4I CCNGG 3 cut(s) 661, 1474, 2159
StyI CCWWGG 4 cut(s) 446, 1532, 2116, 2685
TaaI ACNGT 8 cut(s) 328, 639, 1315, 1642, 1903, 2054, 2199, 2565
TaqI TCGA 6 cut(s) 12, 655, 883, 1149, 1883, 2761
TatI WGTACW 3 cut(s) 280, 369, 3046
TauI GCSGC 1 cut(s) 1963
TfiI GAWTC 6 cut(s) 298, 526, 668, 1034, 1706, 2279
TseFI GTSAC 3 cut(s) 316, 1315, 2900
Tsp45I GTSAC 3 cut(s) 316, 1315, 2900
TspGWI ACGGA 2 cut(s) 291, 842
Vha464I CTTAAG 2 cut(s) 1390, 2837
VpaK11BI GGWCC 2 cut(s) 2467, 2818
XagI CCTNNNNNAGG 2 cut(s) 1521, 2976
XapI RAATTY 5 cut(s) 435, 584, 887, 1492, 2860
XceI RCATGY 3 cut(s) 231, 1185, 2933
XhoI CTCGAG 1 cut(s) 882
XmaJI CCTAGG 1 cut(s) 1532
XmnI GAANNNNTTC 3 cut(s) 8, 302, 1200
XspI CTAG 7 cut(s) 335, 530, 737, 1049, 1533, 2894, 3023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.