MD07G1213800.v1.1

Coronatine-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0028850
Physical Location & Seq
Reverse (-)
29120433 .. 29122079
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1213800.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGAGGATCGAAGCGTGCGGTGTAGGATTTCCGACGTCGTAATGGACTGCGTGATGCCGTATCTCCACGACCCCAAGGACCGTGACGCCGTGTCGTTGGTGTGCAAGCGGTGGTACGAGCTCGACGCGCTCACGCGGAAGCACGTGACCATCGCGCTCTGCTACACCACGACCCCGGATCGGCTGCGGCAGCGGTTTCAGCACCTGGAGTCGCTGAAGCTGAAGGGGAAGCCGAGGGCGGCGATGTTCAATCTGATTCCCGAGGACTGGGGAGGGTTTGTAACGCCGTGGGTGATGGAGATCGCCAACTCGTTCCACCGCTTGAAGTGTCTGCACTTTCGGCGGATGATTGTTAGTGATTCGGACCTGGAGCTCCTGGCTGACTCACGTGGGCGCGTGCTTCAGGTGCTTAAGCTCGACAAGTGCTCAGGGTTTACCACCGGTGGGCTTTTGCACATCGGCCGCTCCTGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009625 GO:0009628 GO:0009639 GO:0009641 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009814 GO:0009861 GO:0009867 GO:0009900 GO:0009901 GO:0009908 GO:0009909 GO:0009966 GO:0009987 GO:0010033 GO:0010118 GO:0010218 GO:0010498 GO:0010646 GO:0019538 GO:0019941 GO:0022414 GO:0022622 GO:0023051 GO:0023052 GO:0030163 GO:0031146 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0042221 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0045087 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048519 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048653 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051239 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0080134 GO:0090567 GO:0098542 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1901701 GO:2000022 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
F-box-like

Protein Analysis

159

Amino Acids

18.32

Weight (kDa)

9.21

Isoelectric Point (pI)

34.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 8 - 48 6.7e-23 F-box
Transp_inhibit PF18791 68 - 113 7.5e-23 Transport inhibitor response 1 protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028850)

Species Orthologous Gene IDs
malus_domestica MD07G1213800.v1.1
rosa_multiflora Rmu_sc0001482.1_g000007

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 39
Acc36I ACCTGC 1 cut(s) 462
AccBSI CCGCTC 1 cut(s) 465
AccII CGCG 4 cut(s) 128, 136, 155, 396
AciI CCGC 9 cut(s) 19, 109, 136, 187, 193, 239, 319, 343, 463
AclWI GGATC 2 cut(s) 15, 186
AcoI YGGCCR 1 cut(s) 460
AcuI CTGAAG 3 cut(s) 236, 242, 386
AcvI CACGTG 2 cut(s) 145, 389
AcyI GRCGYC 2 cut(s) 36, 87
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 3 cut(s) 180, 267, 444
AflII CTTAAG 1 cut(s) 410
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 2 cut(s) 250, 325
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 3 cut(s) 204, 366, 375
AluBI AGCT 4 cut(s) 121, 220, 373, 415
AluI AGCT 4 cut(s) 121, 220, 373, 415
Alw21I GWGCWC 3 cut(s) 123, 375, 428
AlwI GGATC 2 cut(s) 15, 186
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 1 cut(s) 460
ApeKI GCWGC 2 cut(s) 184, 190
AsiGI ACCGGT 1 cut(s) 440
AspLEI GCGC 3 cut(s) 130, 157, 396
AspS9I GGNCC 2 cut(s) 79, 364
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 2 cut(s) 79, 364
BanII GRGCYC 2 cut(s) 123, 375
BbrPI CACGTG 2 cut(s) 145, 389
Bbv12I GWGCWC 3 cut(s) 123, 375, 428
BbvI GCAGC 2 cut(s) 171, 202
BccI CCATC 2 cut(s) 158, 289
BceAI ACGGC 3 cut(s) 43, 74, 271
BciT130I CCWGG 3 cut(s) 206, 368, 377
BcnI CCSGG 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 469
BfrI CTTAAG 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 462
BisI GCNGC 5 cut(s) 185, 188, 191, 240, 463
BlsI GCNGC 5 cut(s) 186, 189, 192, 241, 464
Bme1390I CCNGG 4 cut(s) 176, 206, 368, 377
Bme18I GGWCC 2 cut(s) 79, 364
BmeRI GACNNNNNGTC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 79, 364
BmrFI CCNGG 4 cut(s) 176, 206, 368, 377
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 45
BmuI ACTGGG 1 cut(s) 277
BpmI CTGGAG 2 cut(s) 227, 389
Bpu10I CCTNAGC 1 cut(s) 427
BpuMI CCSGG 1 cut(s) 176
BsaAI YACGTR 2 cut(s) 145, 389
BsaBI GATNNNNATC 1 cut(s) 299
BsaHI GRCGYC 2 cut(s) 36, 87
BsaJI CCNNGG 5 cut(s) 75, 174, 233, 261, 287
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 267, 444
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 1 cut(s) 272
Bse8I GATNNNNATC 1 cut(s) 299
BseBI CCWGG 3 cut(s) 206, 368, 377
BseDI CCNNGG 5 cut(s) 75, 174, 233, 261, 287
BseGI GGATG 1 cut(s) 351
BseJI GATNNNNATC 1 cut(s) 299
BseLI CCNNNNNNNGG 3 cut(s) 180, 267, 444
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 1 cut(s) 272
BseX3I CGGCCG 1 cut(s) 460
BseXI GCAGC 2 cut(s) 171, 202
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 4 cut(s) 128, 136, 155, 396
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 1 cut(s) 462
BshTI ACCGGT 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 463
BsiHKAI GWGCWC 3 cut(s) 123, 375, 428
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 2 cut(s) 176, 441
BslI CCNNNNNNNGG 3 cut(s) 180, 267, 444
BsnI GGCC 1 cut(s) 462
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 3 cut(s) 123, 375, 428
Bsp143I GATC 3 cut(s) 7, 178, 300
BspACI CCGC 9 cut(s) 19, 109, 136, 187, 193, 239, 319, 343, 463
BspANI GGCC 1 cut(s) 462
BspCNI CTCAG 1 cut(s) 440
BspFNI CGCG 4 cut(s) 128, 136, 155, 396
BspMAI CTGCAG 1 cut(s) 473
BspMI ACCTGC 1 cut(s) 462
BspPI GGATC 2 cut(s) 15, 186
BspTI CTTAAG 1 cut(s) 410
BsrBI CCGCTC 1 cut(s) 465
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 5 cut(s) 75, 174, 233, 261, 287
BssMI GATC 3 cut(s) 7, 178, 300
BssNI GRCGYC 2 cut(s) 36, 87
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 3 cut(s) 206, 368, 377
Bst4CI ACNGT 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 36, 87
BstAFI CTTAAG 1 cut(s) 410
BstBAI YACGTR 2 cut(s) 145, 389
BstC8I GCNNGC 3 cut(s) 17, 107, 398
BstDEI CTNAG 1 cut(s) 427
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 351
BstFNI CGCG 4 cut(s) 128, 136, 155, 396
BstHHI GCGC 3 cut(s) 130, 157, 396
BstKTI GATC 3 cut(s) 10, 181, 303
BstMBI GATC 3 cut(s) 7, 178, 300
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 6 cut(s) 127, 190, 199, 340, 406, 468
BstNI CCWGG 3 cut(s) 206, 368, 377
BstSCI CCNGG 4 cut(s) 174, 204, 366, 375
BstSFI CTRYAG 1 cut(s) 469
BstUI CGCG 4 cut(s) 128, 136, 155, 396
BstV1I GCAGC 2 cut(s) 171, 202
BstZI CGGCCG 1 cut(s) 460
BsuRI GGCC 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 287
BtgZI GCGATG 2 cut(s) 136, 257
BtsCI GGATG 1 cut(s) 351
BveI ACCTGC 1 cut(s) 462
Cac8I GCNNGC 3 cut(s) 17, 107, 398
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 3 cut(s) 130, 157, 396
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 2 cut(s) 79, 364
CseI GACGC 2 cut(s) 95, 134
Csp6I GTAC 1 cut(s) 115
CspAI ACCGGT 1 cut(s) 440
CviJI RGCY 9 cut(s) 121, 184, 220, 232, 373, 380, 415, 448, 462
CviKI_1 RGCY 9 cut(s) 121, 184, 220, 232, 373, 380, 415, 448, 462
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 1 cut(s) 427
DpnI GATC 3 cut(s) 9, 180, 302
DpnII GATC 3 cut(s) 7, 178, 300
DriI GACNNNNNGTC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 460
EagI CGGCCG 1 cut(s) 460
Eam1105I GACNNNNNGTC 1 cut(s) 91
EciI GGCGGA 1 cut(s) 358
Ecl136II GAGCTC 2 cut(s) 121, 373
EclXI CGGCCG 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 2 cut(s) 123, 375
Eco47I GGWCC 2 cut(s) 79, 364
Eco52I CGGCCG 1 cut(s) 460
Eco53kI GAGCTC 2 cut(s) 121, 373
Eco57I CTGAAG 3 cut(s) 236, 242, 386
Eco72I CACGTG 2 cut(s) 145, 389
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 2 cut(s) 121, 373
EcoRII CCWGG 3 cut(s) 204, 366, 375
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 2 cut(s) 123, 375
ErhI CCWWGG 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 185, 188, 191, 240, 463
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 2 cut(s) 123, 375
Fsp4HI GCNGC 5 cut(s) 185, 188, 191, 240, 463
GlaI GCGC 3 cut(s) 129, 156, 395
GluI GCNGC 5 cut(s) 185, 188, 191, 240, 463
GsuI CTGGAG 2 cut(s) 227, 389
HaeIII GGCC 1 cut(s) 462
HapII CCGG 2 cut(s) 176, 441
HgaI GACGC 2 cut(s) 95, 134
HhaI GCGC 3 cut(s) 130, 157, 396
Hin1I GRCGYC 2 cut(s) 36, 87
Hin6I GCGC 3 cut(s) 128, 155, 394
HinP1I GCGC 3 cut(s) 128, 155, 394
HinfI GANTC 4 cut(s) 209, 256, 359, 383
HpaII CCGG 2 cut(s) 176, 441
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 3 cut(s) 34, 255, 364
Hpy188III TCNNGA 1 cut(s) 260
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 3 cut(s) 38, 41, 128
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 3 cut(s) 36, 144, 388
HpyCH4V TGCA 4 cut(s) 105, 334, 454, 471
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 190, 199, 340, 406, 468
HpyF3I CTNAG 1 cut(s) 427
HpySE526I ACGT 3 cut(s) 36, 144, 388
Hsp92I GRCGYC 2 cut(s) 36, 87
HspAI GCGC 3 cut(s) 128, 155, 394
Kzo9I GATC 3 cut(s) 7, 178, 300
LmnI GCTCC 3 cut(s) 370, 378, 470
Lsp1109I GCAGC 2 cut(s) 171, 202
LweI GCATC 1 cut(s) 45
MaeII ACGT 3 cut(s) 36, 144, 388
MaeIII GTNAC 3 cut(s) 83, 145, 280
MalI GATC 3 cut(s) 9, 180, 302
MbiI CCGCTC 1 cut(s) 465
MboI GATC 3 cut(s) 7, 178, 300
MhlI GDGCHC 3 cut(s) 123, 375, 428
MlyI GAGTC 2 cut(s) 218, 377
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 3 cut(s) 228, 256, 266
MseI TTAA 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 193
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 2 cut(s) 176, 441
MspR9I CCNGG 4 cut(s) 176, 206, 368, 377
MvaI CCWGG 3 cut(s) 206, 368, 377
MvnI CGCG 4 cut(s) 128, 136, 155, 396
MwoI GCNNNNNNNGC 6 cut(s) 127, 190, 199, 340, 406, 468
NciI CCSGG 1 cut(s) 176
NdeII GATC 3 cut(s) 7, 178, 300
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 2 cut(s) 83, 145
PfeI GAWTC 2 cut(s) 256, 359
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 5 cut(s) 186, 189, 192, 241, 464
PleI GAGTC 2 cut(s) 217, 377
PmaCI CACGTG 2 cut(s) 145, 389
PmlI CACGTG 2 cut(s) 145, 389
PpsI GAGTC 2 cut(s) 217, 377
Ppu21I YACGTR 2 cut(s) 145, 389
Psp124BI GAGCTC 2 cut(s) 123, 375
Psp6I CCWGG 3 cut(s) 204, 366, 375
PspCI CACGTG 2 cut(s) 145, 389
PspGI CCWGG 3 cut(s) 204, 366, 375
PspPI GGNCC 2 cut(s) 79, 364
PstI CTGCAG 1 cut(s) 473
PstNI CAGNNNCTG 1 cut(s) 205
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SacI GAGCTC 2 cut(s) 123, 375
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 5 cut(s) 185, 188, 191, 240, 463
Sau3AI GATC 3 cut(s) 7, 178, 300
Sau96I GGNCC 2 cut(s) 79, 364
SbfI CCTGCAGG 1 cut(s) 473
SchI GAGTC 2 cut(s) 218, 377
ScrFI CCNGG 4 cut(s) 176, 206, 368, 377
SdaI CCTGCAGG 1 cut(s) 473
SduI GDGCHC 3 cut(s) 123, 375, 428
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 1 cut(s) 469
SgrAI CRCCGGYG 1 cut(s) 440
SinI GGWCC 2 cut(s) 79, 364
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse8387I CCTGCAGG 1 cut(s) 473
SsiI CCGC 9 cut(s) 19, 109, 136, 187, 193, 239, 319, 343, 463
SstI GAGCTC 2 cut(s) 123, 375
StyD4I CCNGG 4 cut(s) 174, 204, 366, 375
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 3 cut(s) 39, 147, 391
TaqI TCGA 3 cut(s) 10, 123, 417
TauI GCSGC 3 cut(s) 190, 242, 465
TfiI GAWTC 2 cut(s) 256, 359
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TseFI GTSAC 2 cut(s) 83, 145
TseI GCWGC 2 cut(s) 184, 190
Tsp45I GTSAC 2 cut(s) 83, 145
Vha464I CTTAAG 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 79, 364
ZraI GACGTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.