MD07G1269800.v1.1

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0028852
Physical Location & Seq
Forward (+)
33714070 .. 33715161
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1269800.v1.1.491

Sequence Viewer

Length: 615 bp
ATGATCAGCCACAGACGCTTTAGAGAATTCTCAGCCTTTGTTTTGTCCCTTAAAATTCTCTTCCCCTCTTCCCCTCCGCAATTTTCTGGTCTCAGCCTTTCCCCCCAAAAACAAGGATTTCTCCTTCTGCTGCTATACTTTCCATCTCTCTTTCTTTTCCGGCTTTCTTCTTCATTTCGCACTACGCTCCGTCTCTTTTCCGACGACAAAATGGCGTCCGGCATGGCCGTGAATGATGAATGCAAGCTCAAGTTCGTGGAGCTAAAAGCGAAGAGAAACTATCGATTCATCGTATTCAAGATTGACAAACAAGAAGTGGTGGTAGAGAAAGTTGGCAGCCCGGATGAAACTTACGAGGATTTCGCGGCGTCGCTGCCTGCTGATGAGTGCCGCTATGCTGTCTTTGATTTTGATTTCACCACTGCTGAGAACTGCCAGAAAAGCAAGATTTTCTTCATTGCATGGTCTCCTGAGACATCGAAGGTGAGAATGAAGATGGTGTATGCAAGCTCTAAAGATCGGTTCAAGAGGGAACTTGACGGCATTCAGGTCGAGTTGCAGGCAACTGATCCGAGCGAAATGAGCCTCGACATTATTAAAGGCCGAGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

205

Amino Acids

23.52

Weight (kDa)

8.91

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 74 - 202 1.4e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028852)

Species Orthologous Gene IDs
malus_domestica MD07G1269800.v1.1
pyrus_communis pycom07g24690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 365
AciI CCGC 3 cut(s) 77, 365, 391
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 2 cut(s) 26, 54
AcyI GRCGYC 2 cut(s) 215, 368
AgsI TTSAA 2 cut(s) 298, 526
AluBI AGCT 4 cut(s) 247, 262, 510, 608
AluI AGCT 4 cut(s) 247, 262, 510, 608
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 4 cut(s) 95, 197, 467, 471
AlwI GGATC 1 cut(s) 563
AoxI GGCC 2 cut(s) 225, 601
ApeKI GCWGC 3 cut(s) 130, 336, 373
ApoI RAATTY 2 cut(s) 26, 54
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 409, 496
BanII GRGCYC 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 3 cut(s) 117, 348, 360
BccI CCATC 2 cut(s) 151, 490
BceAI ACGGC 2 cut(s) 212, 556
BcgI CGANNNNNNTGC 2 cut(s) 532, 566
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 4 cut(s) 95, 197, 467, 471
BisI GCNGC 5 cut(s) 131, 337, 366, 374, 391
BlsI GCNGC 5 cut(s) 132, 338, 367, 375, 392
Bme1390I CCNGG 1 cut(s) 341
BmrFI CCNGG 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 341
Bsa29I ATCGAT 1 cut(s) 283
BsaHI GRCGYC 2 cut(s) 215, 368
BsaI GGTCTC 2 cut(s) 95, 471
Bse3DI GCAATG 1 cut(s) 456
BseCI ATCGAT 1 cut(s) 283
BseGI GGATG 1 cut(s) 349
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 4 cut(s) 45, 106, 417, 462
BseXI GCAGC 3 cut(s) 117, 348, 360
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 2 cut(s) 227, 603
BshVI ATCGAT 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 610
BsiSI CCGG 3 cut(s) 160, 219, 341
BslFI GGGAC 1 cut(s) 31
BsmAI GTCTC 4 cut(s) 95, 197, 467, 471
BsmBI CGTCTC 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 31
BsmI GAATGC 2 cut(s) 245, 543
BsnI GGCC 2 cut(s) 227, 603
Bso31I GGTCTC 2 cut(s) 95, 471
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 3 cut(s) 3, 517, 568
BspACI CCGC 3 cut(s) 77, 365, 391
BspANI GGCC 2 cut(s) 227, 603
BspCNI CTCAG 4 cut(s) 44, 105, 418, 463
BspDI ATCGAT 1 cut(s) 283
BspFNI CGCG 1 cut(s) 365
BspPI GGATC 1 cut(s) 563
BspTNI GGTCTC 2 cut(s) 95, 471
BsrDI GCAATG 1 cut(s) 456
BssMI GATC 3 cut(s) 3, 517, 568
BssNI GRCGYC 2 cut(s) 215, 368
Bst6I CTCTTC 3 cut(s) 65, 73, 266
BstACI GRCGYC 2 cut(s) 215, 368
BstC8I GCNNGC 4 cut(s) 245, 378, 508, 561
BstDEI CTNAG 4 cut(s) 31, 92, 426, 471
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 365
BstKTI GATC 3 cut(s) 6, 520, 571
BstMAI GTCTC 4 cut(s) 95, 197, 467, 471
BstMBI GATC 3 cut(s) 3, 517, 568
BstMWI GCNNNNNNNGC 3 cut(s) 15, 441, 582
BstSCI CCNGG 1 cut(s) 339
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 3 cut(s) 117, 348, 360
Bsu15I ATCGAT 1 cut(s) 283
BsuRI GGCC 2 cut(s) 227, 603
BsuTUI ATCGAT 1 cut(s) 283
BtsCI GGATG 1 cut(s) 349
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 4 cut(s) 245, 378, 508, 561
ClaI ATCGAT 1 cut(s) 283
CseI GACGC 3 cut(s) 24, 204, 357
CviAII CATG 2 cut(s) 223, 462
DdeI CTNAG 4 cut(s) 31, 92, 426, 471
DpnI GATC 3 cut(s) 5, 519, 570
DpnII GATC 3 cut(s) 3, 517, 568
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 3 cut(s) 65, 73, 266
EarI CTCTTC 3 cut(s) 65, 73, 266
Ecl136II GAGCTC 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 610
Eco31I GGTCTC 2 cut(s) 95, 471
Eco53kI GAGCTC 1 cut(s) 608
EcoICRI GAGCTC 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 610
Esp3I CGTCTC 1 cut(s) 197
FaeI CATG 2 cut(s) 226, 465
FaiI YATR 5 cut(s) 136, 224, 396, 463, 504
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 1 cut(s) 31
FatI CATG 2 cut(s) 222, 461
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 5 cut(s) 131, 337, 366, 374, 391
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 5 cut(s) 131, 337, 366, 374, 391
GluI GCNGC 5 cut(s) 131, 337, 366, 374, 391
HaeIII GGCC 2 cut(s) 227, 603
HapII CCGG 3 cut(s) 160, 219, 341
HgaI GACGC 3 cut(s) 24, 204, 357
Hin1I GRCGYC 2 cut(s) 215, 368
Hin1II CATG 2 cut(s) 226, 465
HinfI GANTC 1 cut(s) 285
HpaII CCGG 3 cut(s) 160, 219, 341
HphI GGTGA 2 cut(s) 409, 496
Hpy188I TCNGA 2 cut(s) 202, 573
Hpy188III TCNNGA 3 cut(s) 298, 470, 526
Hpy99I CGWCG 2 cut(s) 206, 373
HpyAV CCTTC 2 cut(s) 134, 475
HpyCH4V TGCA 4 cut(s) 243, 461, 506, 559
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 441, 582
HpyF3I CTNAG 4 cut(s) 31, 92, 426, 471
Hsp92I GRCGYC 2 cut(s) 215, 368
Hsp92II CATG 2 cut(s) 226, 465
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 517, 568
LmnI GCTCC 2 cut(s) 192, 259
LpnPI CCDG 9 cut(s) 72, 173, 232, 354, 390, 449, 483, 533, 545
Lsp1109I GCAGC 3 cut(s) 117, 348, 360
MalI GATC 3 cut(s) 5, 519, 570
MboI GATC 3 cut(s) 3, 517, 568
MboII GAAGA 7 cut(s) 52, 60, 159, 162, 283, 445, 505
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 3 cut(s) 26, 54, 80
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 5 cut(s) 76, 84, 349, 522, 596
MseI TTAA 3 cut(s) 51, 597, 613
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 3 cut(s) 160, 219, 341
MspR9I CCNGG 1 cut(s) 341
Mva1269I GAATGC 2 cut(s) 245, 543
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 3 cut(s) 15, 441, 582
NciI CCSGG 1 cut(s) 341
NdeII GATC 3 cut(s) 3, 517, 568
NlaIII CATG 2 cut(s) 226, 465
PctI GAATGC 2 cut(s) 245, 543
PfeI GAWTC 1 cut(s) 285
PkrI GCNGC 5 cut(s) 132, 338, 367, 375, 392
Psp124BI GAGCTC 1 cut(s) 610
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 610
SaqAI TTAA 3 cut(s) 51, 597, 613
SatI GCNGC 5 cut(s) 131, 337, 366, 374, 391
Sau3AI GATC 3 cut(s) 3, 517, 568
ScrFI CCNGG 1 cut(s) 341
SduI GDGCHC 1 cut(s) 610
SetI ASST 6 cut(s) 249, 264, 486, 512, 552, 610
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 3 cut(s) 26, 54, 80
SsiI CCGC 3 cut(s) 77, 365, 391
SstI GAGCTC 1 cut(s) 610
StyD4I CCNGG 1 cut(s) 339
TaqI TCGA 4 cut(s) 283, 479, 552, 588
TasI AATT 3 cut(s) 26, 54, 80
TauI GCSGC 2 cut(s) 368, 393
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 3 cut(s) 51, 597, 613
Tru9I TTAA 3 cut(s) 51, 597, 613
TscAI CASTG 1 cut(s) 427
TseI GCWGC 3 cut(s) 130, 336, 373
TspDTI ATGAA 6 cut(s) 162, 252, 277, 360, 445, 506
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 1 cut(s) 427
XapI RAATTY 2 cut(s) 26, 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.