MD08G1170500.v1.1

UPF0695 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0028646
Physical Location & Seq
Forward (+)
20490316 .. 20491612
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1170500.v1.1.491

Sequence Viewer

Length: 768 bp
ATGAGGAAACGTACTTGTCAAGTAGATTATTTGACGCGCAGCATTGTGCAGATCTTAACGAGATATCTGCTGCAAAAGTTGTTTAGCCCTGGAGTTGCTGGTGTAACAAGCAACCAAACGATTCTCTATCTTGATCTTCCCTCTAATATGAGATATATCCTATACGTCCGACAATTTGGCCATTCGAATTACTTAGGAAGCCTTACAACTTTCAACGGTTGGAACCAGAAAATGCTTGAACTCAGTGTTGAACACCATTGGGTGTTTGCTCTGCTCGGTTTTCTAATGGCTCAAATTGCTGGACTAATCCTTCTCTTTTATGTGACGGATTATATCGGTTTGTTTCTTGCGGCCTACTCAATCATTTTTAGGGTAGAGACAGCAAGGGGTTTTAGGCATCTTCTTGATAGGAGCTTAATATGTGGTGTCACTAGCTCTAGAAGGAGCTCGAGGGTTGATAGCTATAAGCGTCACCTGGTAGCTTTAGCAGTATTCTTGCTTATGCTAGGTGCTCTATGGGGTGTGAGTGGAATATTGTTGAGAGAGGAATTTAATAGCGACAGCAGCGAGGTGCAACTGTGGTTGGGTTGCATAGTTGGACCTCTTGGTGTGTGGATCCAGTGGTTCTTAGCCTGGCTTAATGGACGTGGATTAGGGATGGCGGGGTTGTTTAAATGGGTTCTATTTGGTACTCTTAAGTGCCAATGTTTCTGCAGCTTGTATCATGGTTGCCCTGTCTACTGTGAAGAAAGCGGTAAGTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRCB

Protein Analysis

256

Amino Acids

29.08

Weight (kDa)

9.25

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028646)

Species Orthologous Gene IDs
malus_domestica MD08G1170500.v1.1
pyrus_communis pycom08g14600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 738
AccII CGCG 1 cut(s) 37
AciI CCGC 3 cut(s) 350, 662, 753
AclWI GGATC 2 cut(s) 610, 623
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 548
AfaI GTAC 2 cut(s) 13, 691
AflII CTTAAG 1 cut(s) 695
AgsI TTSAA 3 cut(s) 214, 239, 251
AjiI CACGTC 1 cut(s) 647
AjnI CCWGG 3 cut(s) 88, 474, 632
AluBI AGCT 6 cut(s) 414, 435, 447, 462, 482, 717
AluI AGCT 6 cut(s) 414, 435, 447, 462, 482, 717
Alw21I GWGCWC 2 cut(s) 449, 514
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 2 cut(s) 610, 623
Ama87I CYCGRG 1 cut(s) 448
AoxI GGCC 2 cut(s) 178, 351
ApeKI GCWGC 4 cut(s) 39, 70, 564, 714
ApoI RAATTY 1 cut(s) 548
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 464
AsuII TTCGAA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 448
AvaII GGWCC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 180
BamHI GGATCC 1 cut(s) 615
BanII GRGCYC 1 cut(s) 449
Bbv12I GWGCWC 2 cut(s) 449, 514
BbvI GCAGC 4 cut(s) 51, 57, 576, 726
BccI CCATC 1 cut(s) 652
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BciT130I CCWGG 3 cut(s) 90, 476, 634
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 3 cut(s) 432, 438, 506
BfmI CTRYAG 1 cut(s) 712
BfrI CTTAAG 1 cut(s) 695
BglII AGATCT 1 cut(s) 51
BisI GCNGC 5 cut(s) 40, 71, 351, 565, 715
BlsI GCNGC 5 cut(s) 41, 72, 352, 566, 716
Bme1390I CCNGG 3 cut(s) 90, 476, 634
Bme18I GGWCC 1 cut(s) 599
BmeT110I CYCGRG 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 2 cut(s) 224, 617
BmrFI CCNGG 3 cut(s) 90, 476, 634
BmsI GCATC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 619
BseBI CCWGG 3 cut(s) 90, 476, 634
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 1 cut(s) 663
BseMII CTCAG 1 cut(s) 256
BseNI ACTGG 1 cut(s) 619
BseXI GCAGC 4 cut(s) 51, 57, 576, 726
BsgI GTGCAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 180, 353
BsiHKAI GWGCWC 2 cut(s) 449, 514
BsiHKCI CYCGRG 1 cut(s) 448
BsmAI GTCTC 1 cut(s) 371
BsnI GGCC 2 cut(s) 180, 353
BsoBI CYCGRG 1 cut(s) 448
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 449, 514
Bsp143I GATC 3 cut(s) 51, 133, 615
BspACI CCGC 3 cut(s) 350, 662, 753
BspANI GGCC 2 cut(s) 180, 353
BspCNI CTCAG 1 cut(s) 255
BspFNI CGCG 1 cut(s) 37
BspLI GGNNCC 2 cut(s) 224, 617
BspMAI CTGCAG 1 cut(s) 716
BspPI GGATC 2 cut(s) 610, 623
BspT104I TTCGAA 1 cut(s) 185
BspTI CTTAAG 1 cut(s) 695
BsrI ACTGG 1 cut(s) 619
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 3 cut(s) 51, 133, 615
Bst2UI CCWGG 3 cut(s) 90, 476, 634
Bst4CI ACNGT 3 cut(s) 218, 579, 743
BstAFI CTTAAG 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 185
BstDEI CTNAG 3 cut(s) 193, 242, 628
BstF5I GGATG 1 cut(s) 663
BstFNI CGCG 1 cut(s) 37
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 3 cut(s) 54, 136, 618
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 3 cut(s) 51, 133, 615
BstMWI GCNNNNNNNGC 2 cut(s) 296, 564
BstNI CCWGG 3 cut(s) 90, 476, 634
BstSCI CCNGG 3 cut(s) 88, 474, 632
BstSFI CTRYAG 1 cut(s) 712
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 4 cut(s) 51, 57, 576, 726
BstX2I RGATCY 2 cut(s) 51, 615
BstYI RGATCY 2 cut(s) 51, 615
BsuRI GGCC 2 cut(s) 180, 353
BtrI CACGTC 1 cut(s) 647
BtsCI GGATG 1 cut(s) 663
BtsIMutI CAGTG 2 cut(s) 250, 626
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 599
CseI GACGC 2 cut(s) 43, 458
CsiI ACCWGGT 1 cut(s) 474
Csp6I GTAC 2 cut(s) 12, 690
CviAII CATG 1 cut(s) 725
CviQI GTAC 2 cut(s) 12, 690
DdeI CTNAG 3 cut(s) 193, 242, 628
DpnI GATC 3 cut(s) 53, 135, 617
DpnII GATC 3 cut(s) 51, 133, 615
DraI TTTAAA 1 cut(s) 673
EaeI YGGCCR 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 449
Eco32I GATATC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 599
Eco53kI GAGCTC 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 447
EcoRII CCWGG 3 cut(s) 88, 474, 632
EcoRV GATATC 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 449
FaeI CATG 1 cut(s) 728
FatI CATG 1 cut(s) 724
FauI CCCGC 1 cut(s) 655
FblI GTMKAC 1 cut(s) 738
Fnu4HI GCNGC 5 cut(s) 40, 71, 351, 565, 715
FokI GGATG 1 cut(s) 670
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 5 cut(s) 40, 71, 351, 565, 715
FspBI CTAG 3 cut(s) 432, 438, 506
GlaI GCGC 1 cut(s) 38
GluI GCNGC 5 cut(s) 40, 71, 351, 565, 715
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 2 cut(s) 180, 353
HgaI GACGC 2 cut(s) 43, 458
HhaI GCGC 1 cut(s) 39
Hin1II CATG 1 cut(s) 728
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 1 cut(s) 121
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 3 cut(s) 131, 404, 438
Hpy8I GTNNAC 1 cut(s) 739
HpyAV CCTTC 2 cut(s) 320, 435
HpyCH4III ACNGT 3 cut(s) 218, 579, 743
HpyCH4IV ACGT 3 cut(s) 10, 165, 646
HpyCH4V TGCA 5 cut(s) 49, 73, 574, 591, 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 564
HpyF3I CTNAG 3 cut(s) 193, 242, 628
HpySE526I ACGT 3 cut(s) 10, 165, 646
Hsp92II CATG 1 cut(s) 728
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 51, 133, 615
LmnI GCTCC 2 cut(s) 411, 444
Lsp1109I GCAGC 4 cut(s) 51, 57, 576, 726
LweI GCATC 1 cut(s) 406
MabI ACCWGGT 1 cut(s) 474
MaeI CTAG 3 cut(s) 432, 438, 506
MaeII ACGT 3 cut(s) 10, 165, 646
MaeIII GTNAC 4 cut(s) 103, 322, 427, 470
MalI GATC 3 cut(s) 53, 135, 617
MboI GATC 3 cut(s) 51, 133, 615
MboII GAAGA 3 cut(s) 128, 392, 758
MflI RGATCY 2 cut(s) 51, 615
MhlI GDGCHC 2 cut(s) 449, 514
MlsI TGGCCA 1 cut(s) 180
MluCI AATT 4 cut(s) 173, 187, 294, 548
MluNI TGGCCA 1 cut(s) 180
MmeI TCCRAC 3 cut(s) 193, 200, 577
MnlI CCTC 5 cut(s) 151, 444, 538, 562, 612
Mox20I TGGCCA 1 cut(s) 180
MscI TGGCCA 1 cut(s) 180
MseI TTAA 6 cut(s) 56, 416, 552, 639, 672, 696
Msp20I TGGCCA 1 cut(s) 180
MspCI CTTAAG 1 cut(s) 695
MspR9I CCNGG 3 cut(s) 90, 476, 634
MvaI CCWGG 3 cut(s) 90, 476, 634
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 2 cut(s) 296, 564
NdeII GATC 3 cut(s) 51, 133, 615
NlaIII CATG 1 cut(s) 728
NlaIV GGNNCC 2 cut(s) 224, 617
NmuCI GTSAC 3 cut(s) 322, 427, 470
NspV TTCGAA 1 cut(s) 185
PaeR7I CTCGAG 1 cut(s) 448
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 5 cut(s) 41, 72, 352, 566, 716
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 3 cut(s) 88, 474, 632
PspGI CCWGG 3 cut(s) 88, 474, 632
PspN4I GGNNCC 2 cut(s) 224, 617
PspPI GGNCC 1 cut(s) 599
PspXI VCTCGAGB 1 cut(s) 448
PstI CTGCAG 1 cut(s) 716
PsuI RGATCY 2 cut(s) 51, 615
RsaI GTAC 2 cut(s) 13, 691
RsaNI GTAC 2 cut(s) 12, 690
SacI GAGCTC 1 cut(s) 449
SaqAI TTAA 6 cut(s) 56, 416, 552, 639, 672, 696
SatI GCNGC 5 cut(s) 40, 71, 351, 565, 715
Sau3AI GATC 3 cut(s) 51, 133, 615
Sau96I GGNCC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 90, 476, 634
SduI GDGCHC 2 cut(s) 449, 514
SexAI ACCWGGT 1 cut(s) 474
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 1 cut(s) 712
Sfr274I CTCGAG 1 cut(s) 448
SfuI TTCGAA 1 cut(s) 185
SinI GGWCC 1 cut(s) 599
SlaI CTCGAG 1 cut(s) 448
SmlI CTYRAG 2 cut(s) 448, 695
SmoI CTYRAG 2 cut(s) 448, 695
Sse9I AATT 4 cut(s) 173, 187, 294, 548
SsiI CCGC 3 cut(s) 350, 662, 753
SspI AATATT 1 cut(s) 534
SspMI CTAG 3 cut(s) 432, 438, 506
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 3 cut(s) 88, 474, 632
TaaI ACNGT 3 cut(s) 218, 579, 743
TaiI ACGT 3 cut(s) 13, 168, 649
TaqI TCGA 2 cut(s) 185, 449
TasI AATT 4 cut(s) 173, 187, 294, 548
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 6 cut(s) 56, 416, 552, 639, 672, 696
Tru9I TTAA 6 cut(s) 56, 416, 552, 639, 672, 696
TscAI CASTG 2 cut(s) 250, 626
TseFI GTSAC 3 cut(s) 322, 427, 470
TseI GCWGC 4 cut(s) 39, 70, 564, 714
Tsp45I GTSAC 3 cut(s) 322, 427, 470
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 2 cut(s) 250, 626
Vha464I CTTAAG 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 1 cut(s) 548
XbaI TCTAGA 1 cut(s) 437
XhoI CTCGAG 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 738
XspI CTAG 3 cut(s) 432, 438, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.