Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0016206

Physical Location & Seq

Genomic Coordinates Reverse (-)

30874319 .. 30877630

Gene Structure 3312 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1243400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGGGTTGTGCGACGCTGGACCTCGCCGGAGCTGTTTCTCTCAAGCTCAACAACGATACCCACTTCCTCCGCTGCTCTCCGAAGCCTTTCCTCTCCTTTGTGGAGCTCATCCGCTCTCGCCGCCATCCCAAGGGCCCCCCTTCCAACCTTCTCAGCTGTCGTGCTGACAAATCACGGCCGTCCTTTTCCCTTCGCGCCCAATCAACCACTACTGTTGCTCCTGAAGAAGAGCGGGTGGTTGTTTTGGTGATTGGTGGAGGGGGAAGGGAGCACGCTCTTTGCTACGCATTGAAGCGGTCCCCTTCCTGTGACGCCGTCTTTTGCGCTCCTGGCAATCCCGGAATATCCAGCTCCGGGGATGCCACTTGTATTTCTGACATCGACATCTCTGATAGCTCGGCAGTCATATCATTTTGCCAGAAATGGCGTGTGGGGCTTGTCGTTGTTGGACCTGAGGCCCCTCTTGTTGCTGGTCTCGTGAATGATTTGCTCAAGGCTGGAATCCATGCTTTCGGCCCTTCATCTGAGGCTGCTGCTTTGGAAGGGTCCAAGAACTTCATGAAGAGTTTGTGCGACAAGTACAGAATACCTACTGCTAAGTATCAAACGTTTACAGACCCGTCTGCTGCAAAGCAATATATACAAGAGCAAGGGGTTCCTATTGTTGTTAAAGCAGATGGATTGGCTGCTGGAAAAGGAGTTATTGTCGCTATGACTTTGAACGAGGCTTTTGATGCGGTTGATGCAATGCTAGTAAAAGGTTCTTTTGGGTCTGCAGGTTGTCGTGTCATTGTTGAGGAATATCTGGAAGGTGAAGAAGCGTCTTTCTTTGCGCTGGTGGATGGAGAGAATGCTATACCTCTGGAATCTGCTCAGGACCATAAACGCGTTGGGGATGGCGATACGGGGCCAAATACTGGTGGAATGGGTGCATACTCACCTGCTCCCATCCTAACTAAAGAACTTGAAGATTTAGTCATGAAATCCATCATTTACCCCACAGTGAAAGGCATGTCAGCCGAGGGCTGTAAGTTTGTTGGGGTTTTATATGCTGGACTCATGATCGAAAAGAAGTCTGGCTTACCTAAACTGATAGAGTACAATGTGCGTTTTGGCGATCCAGAGTGTCAGGTTTTAATGGTTCGCTTGGAGTCTGATCTGGCACAAGTTCTACTTGCAGCTTGTAGAGGACAGCTAAGCGAAGTGTCGTTGAACTGGTCGGCTGGGTCAGCCATGGTGGTGGTAATGGCAAGTAAAGGGTACCCAGGATCATATGAGAAGGGAAGTGTGATCCGGAACCTTGATGAAGCAGAAGCTGTTGCCCCATCTGTTAAGGTATTTCATGCGGGAACTGCACTGGATTCAGATGGCAATTTCATTGCTGCTGGAGGGCGTGTCCTTGGGGTGACTGCCAAGGGAATAGATCTCAAAGAGGCACGCGACCGGGCATATCAAGGCATTGAAGAAATTAATTGGCCAGGAGGGTTCTACCGCCGTGATATTGGCTGGAGAGCCCTTCCTCTTCCTCAGAAACAGTTTGTGACAGGAGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 ko01110 ko01130 map00230 map01100 map01110 map01130

Pfam Domains

Protein Families

GARS_A GARS_C GARS_N

Protein Analysis

518

Amino Acids

54.79

Weight (kDa)

5.85

Isoelectric Point (pI)

39.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GARS_N	PF02844	81 - 181	8.2e-33	Phosphoribosylglycinamide synthetase, N domain
GARS_A	PF01071	182 - 376	1.6e-84	Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain
GARS_C	PF02843	411 - 504	1.5e-32	Phosphoribosylglycinamide synthetase, C domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016206)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09830
malus_domestica	MD08G1243400.v1.1 MD15G1437700.v1.1
prunus_persica	Prupe.1G580800_v2.0.a1
pyrus_communis	pycom08g21110 pycom15g38590
rosa_laevigata	RLG00000026323
rosa_multiflora	Rmu_sc0016989.1_g000004
rosa_roxburghii	Rroxscaffold_4G00279600
rosa_rugosa	Rorug01G0418600
rosa_samantha	Rh1AG443500 Rh1BG398400 Rh1CG411800 Rh1DG429100
rosa_wichuraiana	Rw1G038510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	949
Acc36I	ACCTGC	2 cut(s)	767, 949
Acc65I	GGTACC	1 cut(s)	1260
AccB1I	GGYRCC	1 cut(s)	1260
AccB7I	CCANNNNNTGG	1 cut(s)	917
AccBSI	CCGCTC	2 cut(s)	114, 232
AccII	CGCG	3 cut(s)	195, 888, 1440
AccIII	TCCGGA	1 cut(s)	1293
AciI	CCGC	8 cut(s)	70, 112, 121, 232, 295, 737, 1346, 1492
AclI	AACGTT	1 cut(s)	608
AclWI	GGATC	3 cut(s)	1112, 1276, 1285
AcoI	YGGCCR	2 cut(s)	176, 1475
AcuI	CTGAAG	1 cut(s)	243
AcyI	GRCGYC	1 cut(s)	312
AfaI	GTAC	3 cut(s)	581, 1100, 1262
AfiI	CCNNNNNNNGG	3 cut(s)	130, 354, 917
AflIII	ACRYGT	1 cut(s)	886
AgsI	TTSAA	5 cut(s)	292, 721, 968, 1213, 1463
AjnI	CCWGG	3 cut(s)	328, 1264, 1477
AluBI	AGCT	9 cut(s)	32, 46, 106, 156, 351, 396, 1181, 1195, 1316
AluI	AGCT	9 cut(s)	32, 46, 106, 156, 351, 396, 1181, 1195, 1316
Alw21I	GWGCWC	2 cut(s)	108, 273
Alw26I	GTCTC	1 cut(s)	479
AlwI	GGATC	3 cut(s)	1112, 1276, 1285
AlwNI	CAGNNNCTG	1 cut(s)	1316
Aor13HI	TCCGGA	1 cut(s)	1293
AoxI	GGCC	6 cut(s)	133, 176, 456, 514, 908, 1475
ApaI	GGGCCC	1 cut(s)	137
ApeKI	GCWGC	7 cut(s)	72, 530, 533, 626, 686, 1178, 1382
AseI	ATTAAT	1 cut(s)	1470
Asp700I	GAANNNNTTC	1 cut(s)	86
Asp718I	GGTACC	1 cut(s)	1260
AspLEI	GCGC	3 cut(s)	197, 326, 835
AsuC2I	CCSGG	3 cut(s)	339, 355, 1445
AsuHPI	GGTGA	4 cut(s)	259, 824, 930, 1417
AvaII	GGWCC	5 cut(s)	19, 297, 449, 546, 877
AxyI	CCTNAGG	1 cut(s)	453
BaeGI	GKGCMC	1 cut(s)	137
BalI	TGGCCA	1 cut(s)	1477
BanI	GGYRCC	1 cut(s)	1260
BanII	GRGCYC	3 cut(s)	108, 137, 1516
BauI	CACGAG	1 cut(s)	476
Bbv12I	GWGCWC	2 cut(s)	108, 273
BbvI	GCAGC	7 cut(s)	59, 517, 520, 613, 673, 1190, 1369
BccI	CCATC	8 cut(s)	132, 671, 836, 890, 956, 995, 1333, 1361
BceAI	ACGGC	4 cut(s)	163, 191, 299, 1479
BciT130I	CCWGG	3 cut(s)	330, 1266, 1479
BcnI	CCSGG	3 cut(s)	339, 355, 1445
BcoDI	GTCTC	1 cut(s)	479
BfaI	CTAG	1 cut(s)	752
BfmI	CTRYAG	1 cut(s)	774
BfuAI	ACCTGC	2 cut(s)	767, 949
BglII	AGATCT	1 cut(s)	1423
BisI	GCNGC	8 cut(s)	73, 121, 531, 534, 627, 687, 1179, 1383
BlpI	GCTNAGC	1 cut(s)	1196
BlsI	GCNGC	8 cut(s)	74, 122, 532, 535, 628, 688, 1180, 1384
Bme1390I	CCNGG	6 cut(s)	330, 339, 355, 1266, 1445, 1479
Bme18I	GGWCC	5 cut(s)	19, 297, 449, 546, 877
BmiI	GGNNCC	9 cut(s)	135, 136, 299, 459, 547, 657, 909, 1262, 1298
BmrFI	CCNGG	6 cut(s)	330, 339, 355, 1266, 1445, 1479
BmsI	GCATC	3 cut(s)	349, 724, 733
BpmI	CTGGAG	2 cut(s)	1407, 1528
Bpu10I	CCTNAGC	1 cut(s)	873
Bpu1102I	GCTNAGC	1 cut(s)	1196
BpuEI	CTTGAG	2 cut(s)	26, 476
BpuMI	CCSGG	3 cut(s)	339, 355, 1445
BsaHI	GRCGYC	1 cut(s)	312
BsaI	GGTCTC	1 cut(s)	479
BsaJI	CCNNGG	7 cut(s)	129, 354, 1020, 1233, 1264, 1399, 1413
BsaWI	WCCGGW	1 cut(s)	1293
BsaXI	ACNNNNNCTCC	6 cut(s)	202, 232, 690, 720, 1380, 1410
Bsc4I	CCNNNNNNNGG	3 cut(s)	130, 354, 917
Bse1I	ACTGG	3 cut(s)	922, 1220, 1362
Bse21I	CCTNAGG	1 cut(s)	453
Bse3DI	GCAATG	2 cut(s)	753, 1377
BseAI	TCCGGA	1 cut(s)	1293
BseBI	CCWGG	3 cut(s)	330, 1266, 1479
BseDI	CCNNGG	7 cut(s)	129, 354, 1020, 1233, 1264, 1399, 1413
BseGI	GGATG	6 cut(s)	108, 124, 364, 847, 901, 948
BseLI	CCNNNNNNNGG	3 cut(s)	130, 354, 917
BseMI	GCAATG	2 cut(s)	753, 1377
BseMII	CTCAG	5 cut(s)	166, 444, 516, 887, 1541
BseNI	ACTGG	3 cut(s)	922, 1220, 1362
BseSI	GKGCMC	1 cut(s)	137
BseX3I	CGGCCG	1 cut(s)	176
BseXI	GCAGC	7 cut(s)	59, 517, 520, 613, 673, 1190, 1369
BseYI	CCCAGC	1 cut(s)	1223
BsgI	GTGCAG	1 cut(s)	1338
Bsh1236I	CGCG	3 cut(s)	195, 888, 1440
Bsh1285I	CGRYCG	2 cut(s)	179, 1444
BshFI	GGCC	6 cut(s)	135, 178, 458, 516, 910, 1477
BshNI	GGYRCC	1 cut(s)	1260
BsiEI	CGRYCG	2 cut(s)	179, 1444
BsiHKAI	GWGCWC	2 cut(s)	108, 273
BsiSI	CCGG	5 cut(s)	27, 339, 354, 1294, 1444
BslFI	GGGAC	1 cut(s)	283
BslI	CCNNNNNNNGG	3 cut(s)	130, 354, 917
BsmAI	GTCTC	1 cut(s)	479
BsmFI	GGGAC	1 cut(s)	283
BsmI	GAATGC	1 cut(s)	856
BsnI	GGCC	6 cut(s)	135, 178, 458, 516, 910, 1477
Bso31I	GGTCTC	1 cut(s)	479
Bsp120I	GGGCCC	1 cut(s)	133
Bsp1286I	GDGCHC	4 cut(s)	108, 137, 273, 1516
Bsp13I	TCCGGA	1 cut(s)	1293
Bsp143I	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
Bsp1720I	GCTNAGC	1 cut(s)	1196
Bsp19I	CCATGG	1 cut(s)	1233
BspACI	CCGC	8 cut(s)	70, 112, 121, 232, 295, 737, 1346, 1492
BspANI	GGCC	6 cut(s)	135, 178, 458, 516, 910, 1477
BspCNI	CTCAG	5 cut(s)	165, 445, 517, 886, 1540
BspEI	TCCGGA	1 cut(s)	1293
BspFNI	CGCG	3 cut(s)	195, 888, 1440
BspHI	TCATGA	3 cut(s)	558, 978, 1059
BspLI	GGNNCC	9 cut(s)	135, 136, 299, 459, 547, 657, 909, 1262, 1298
BspMAI	CTGCAG	1 cut(s)	778
BspMI	ACCTGC	2 cut(s)	767, 949
BspPI	GGATC	3 cut(s)	1112, 1276, 1285
BspQI	GCTCTTC	1 cut(s)	222
BspT107I	GGYRCC	1 cut(s)	1260
BspTNI	GGTCTC	1 cut(s)	479
BsrBI	CCGCTC	2 cut(s)	114, 232
BsrDI	GCAATG	2 cut(s)	753, 1377
BsrI	ACTGG	3 cut(s)	922, 1220, 1362
BssECI	CCNNGG	7 cut(s)	129, 354, 1020, 1233, 1264, 1399, 1413
BssMI	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
BssNI	GRCGYC	1 cut(s)	312
BssSI	CACGAG	1 cut(s)	476
BssT1I	CCWWGG	4 cut(s)	129, 1233, 1399, 1413
Bst2BI	CACGAG	1 cut(s)	476
Bst2UI	CCWGG	3 cut(s)	330, 1266, 1479
Bst4CI	ACNGT	3 cut(s)	214, 1003, 1536
Bst6I	CTCTTC	3 cut(s)	222, 557, 1527
BstACI	GRCGYC	1 cut(s)	312
BstC8I	GCNNGC	2 cut(s)	273, 1438
BstDEI	CTNAG	7 cut(s)	152, 453, 525, 597, 873, 1196, 1527
BstDSI	CCRYGG	1 cut(s)	1233
BstF5I	GGATG	6 cut(s)	108, 124, 364, 847, 901, 948
BstFNI	CGCG	3 cut(s)	195, 888, 1440
BstHHI	GCGC	3 cut(s)	197, 326, 835
BstKTI	GATC	6 cut(s)	1065, 1120, 1159, 1271, 1293, 1426
BstMAI	GTCTC	1 cut(s)	479
BstMBI	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
BstMCI	CGRYCG	2 cut(s)	179, 1444
BstMWI	GCNNNNNNNGC	7 cut(s)	120, 330, 433, 734, 743, 1229, 1352
BstNI	CCWGG	3 cut(s)	330, 1266, 1479
BstNSI	RCATGY	1 cut(s)	1015
BstSCI	CCNGG	6 cut(s)	328, 337, 353, 1264, 1443, 1477
BstSFI	CTRYAG	1 cut(s)	774
BstSLI	GKGCMC	1 cut(s)	137
BstUI	CGCG	3 cut(s)	195, 888, 1440
BstV1I	GCAGC	7 cut(s)	59, 517, 520, 613, 673, 1190, 1369
BstX2I	RGATCY	1 cut(s)	1423
BstXI	CCANNNNNNTGG	1 cut(s)	1240
BstYI	RGATCY	1 cut(s)	1423
BstZI	CGGCCG	1 cut(s)	176
Bsu36I	CCTNAGG	1 cut(s)	453
BsuRI	GGCC	6 cut(s)	135, 178, 458, 516, 910, 1477
BtgI	CCRYGG	1 cut(s)	1233
BtsCI	GGATG	6 cut(s)	108, 124, 364, 847, 901, 948
BtsIMutI	CAGTG	2 cut(s)	1008, 1355
BveI	ACCTGC	2 cut(s)	767, 949
Cac8I	GCNNGC	2 cut(s)	273, 1438
CaiI	CAGNNNCTG	1 cut(s)	1316
CciI	TCATGA	3 cut(s)	558, 978, 1059
CfoI	GCGC	3 cut(s)	197, 326, 835
CseI	GACGC	3 cut(s)	22, 320, 810
Csp6I	GTAC	3 cut(s)	580, 1099, 1261
CspCI	CAANNNNNGTGG	2 cut(s)	196, 231
CviAII	CATG	7 cut(s)	506, 559, 979, 1012, 1060, 1234, 1343
CviQI	GTAC	3 cut(s)	580, 1099, 1261
DdeI	CTNAG	7 cut(s)	152, 453, 525, 597, 873, 1196, 1527
DpnI	GATC	6 cut(s)	1064, 1119, 1158, 1270, 1292, 1425
DpnII	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
EaeI	YGGCCR	2 cut(s)	176, 1475
EagI	CGGCCG	1 cut(s)	176
Eam1104I	CTCTTC	3 cut(s)	222, 557, 1527
EarI	CTCTTC	3 cut(s)	222, 557, 1527
Ecl136II	GAGCTC	1 cut(s)	106
EclXI	CGGCCG	1 cut(s)	176
Eco130I	CCWWGG	4 cut(s)	129, 1233, 1399, 1413
Eco24I	GRGCYC	3 cut(s)	108, 137, 1516
Eco31I	GGTCTC	1 cut(s)	479
Eco47I	GGWCC	5 cut(s)	19, 297, 449, 546, 877
Eco52I	CGGCCG	1 cut(s)	176
Eco53kI	GAGCTC	1 cut(s)	106
Eco57I	CTGAAG	1 cut(s)	243
Eco81I	CCTNAGG	1 cut(s)	453
EcoICRI	GAGCTC	1 cut(s)	106
EcoO109I	RGGNCCY	3 cut(s)	133, 134, 457
EcoRII	CCWGG	3 cut(s)	328, 1264, 1477
EcoT14I	CCWWGG	4 cut(s)	129, 1233, 1399, 1413
EcoT38I	GRGCYC	3 cut(s)	108, 137, 1516
ErhI	CCWWGG	4 cut(s)	129, 1233, 1399, 1413
FaeI	CATG	7 cut(s)	509, 562, 982, 1015, 1063, 1237, 1346
FalI	AAGNNNNNCTT	4 cut(s)	1064, 1096, 1158, 1190
FaqI	GGGAC	1 cut(s)	283
FatI	CATG	7 cut(s)	505, 558, 978, 1011, 1059, 1233, 1342
FauI	CCCGC	2 cut(s)	225, 1339
FauNDI	CATATG	1 cut(s)	1273
Fnu4HI	GCNGC	8 cut(s)	73, 121, 531, 534, 627, 687, 1179, 1383
FokI	GGATG	6 cut(s)	95, 111, 371, 854, 908, 935
FriOI	GRGCYC	3 cut(s)	108, 137, 1516
Fsp4HI	GCNGC	8 cut(s)	73, 121, 531, 534, 627, 687, 1179, 1383
FspBI	CTAG	1 cut(s)	752
GlaI	GCGC	3 cut(s)	196, 325, 834
GluI	GCNGC	8 cut(s)	73, 121, 531, 534, 627, 687, 1179, 1383
GsaI	CCCAGC	1 cut(s)	1227
GsuI	CTGGAG	2 cut(s)	1407, 1528
HaeIII	GGCC	6 cut(s)	135, 178, 458, 516, 910, 1477
HapII	CCGG	5 cut(s)	27, 339, 354, 1294, 1444
HgaI	GACGC	3 cut(s)	22, 320, 810
HhaI	GCGC	3 cut(s)	197, 326, 835
Hin1I	GRCGYC	1 cut(s)	312
Hin1II	CATG	7 cut(s)	509, 562, 982, 1015, 1063, 1237, 1346
Hin6I	GCGC	3 cut(s)	195, 324, 833
HinP1I	GCGC	3 cut(s)	195, 324, 833
HinfI	GANTC	5 cut(s)	501, 866, 1056, 1151, 1361
HpaII	CCGG	5 cut(s)	27, 339, 354, 1294, 1444
HphI	GGTGA	4 cut(s)	259, 824, 930, 1417
Hpy166II	GTNNAC	1 cut(s)	612
Hpy188I	TCNGA	7 cut(s)	81, 376, 391, 526, 1156, 1366, 1530
Hpy8I	GTNNAC	1 cut(s)	612
Hpy99I	CGWCG	1 cut(s)	16
HpyCH4III	ACNGT	3 cut(s)	214, 1003, 1536
HpyCH4IV	ACGT	1 cut(s)	608
HpyCH4V	TGCA	6 cut(s)	629, 746, 776, 932, 1178, 1355
HpyF10VI	GCNNNNNNNGC	7 cut(s)	120, 330, 433, 734, 743, 1229, 1352
HpyF3I	CTNAG	7 cut(s)	152, 453, 525, 597, 873, 1196, 1527
HpySE526I	ACGT	1 cut(s)	608
Hsp92I	GRCGYC	1 cut(s)	312
Hsp92II	CATG	7 cut(s)	509, 562, 982, 1015, 1063, 1237, 1346
HspAI	GCGC	3 cut(s)	195, 324, 833
Kpn2I	TCCGGA	1 cut(s)	1293
KpnI	GGTACC	1 cut(s)	1264
Kzo9I	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
LguI	GCTCTTC	1 cut(s)	222
LmnI	GCTCC	7 cut(s)	29, 103, 223, 268, 331, 356, 949
Lsp1109I	GCAGC	7 cut(s)	59, 517, 520, 613, 673, 1190, 1369
LweI	GCATC	3 cut(s)	349, 724, 733
MaeI	CTAG	1 cut(s)	752
MaeII	ACGT	1 cut(s)	608
MaeIII	GTNAC	3 cut(s)	308, 1405, 1540
MalI	GATC	6 cut(s)	1064, 1119, 1158, 1270, 1292, 1425
MbiI	CCGCTC	2 cut(s)	114, 232
MboI	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
MboII	GAAGA	7 cut(s)	236, 239, 574, 827, 980, 1475, 1514
MflI	RGATCY	1 cut(s)	1423
MhlI	GDGCHC	4 cut(s)	108, 137, 273, 1516
MlsI	TGGCCA	1 cut(s)	1477
MluCI	AATT	3 cut(s)	1372, 1467, 1471
MluI	ACGCGT	1 cut(s)	886
MluNI	TGGCCA	1 cut(s)	1477
MlyI	GAGTC	2 cut(s)	1050, 1160
MmeI	TCCRAC	2 cut(s)	168, 427
Mox20I	TGGCCA	1 cut(s)	1477
MroI	TCCGGA	1 cut(s)	1293
MroXI	GAANNNNTTC	1 cut(s)	86
MscI	TGGCCA	1 cut(s)	1477
MseI	TTAA	5 cut(s)	669, 1136, 1332, 1470, 1552
MslI	CAYNNNNRTG	1 cut(s)	1238
Msp20I	TGGCCA	1 cut(s)	1477
MspA1I	CMGCKG	2 cut(s)	72, 156
MspI	CCGG	5 cut(s)	27, 339, 354, 1294, 1444
MspR9I	CCNGG	6 cut(s)	330, 339, 355, 1266, 1445, 1479
Mva1269I	GAATGC	1 cut(s)	856
MvaI	CCWGG	3 cut(s)	330, 1266, 1479
MvnI	CGCG	3 cut(s)	195, 888, 1440
MwoI	GCNNNNNNNGC	7 cut(s)	120, 330, 433, 734, 743, 1229, 1352
NciI	CCSGG	3 cut(s)	339, 355, 1445
NcoI	CCATGG	1 cut(s)	1233
NdeI	CATATG	1 cut(s)	1273
NdeII	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
NlaIII	CATG	7 cut(s)	509, 562, 982, 1015, 1063, 1237, 1346
NlaIV	GGNNCC	9 cut(s)	135, 136, 299, 459, 547, 657, 909, 1262, 1298
NmeAIII	GCCGAG	2 cut(s)	377, 1045
NmuCI	GTSAC	3 cut(s)	308, 1405, 1540
NspI	RCATGY	1 cut(s)	1015
PagI	TCATGA	3 cut(s)	558, 978, 1059
PaqCI	CACCTGC	1 cut(s)	949
PciSI	GCTCTTC	1 cut(s)	222
PctI	GAATGC	1 cut(s)	856
PdmI	GAANNNNTTC	1 cut(s)	86
PfeI	GAWTC	3 cut(s)	501, 866, 1361
PflFI	GACNNNGTC	1 cut(s)	314
PflMI	CCANNNNNTGG	1 cut(s)	917
PfoI	TCCNGGA	1 cut(s)	337
PkrI	GCNGC	8 cut(s)	74, 122, 532, 535, 628, 688, 1180, 1384
PleI	GAGTC	2 cut(s)	1050, 1159
PpsI	GAGTC	2 cut(s)	1050, 1159
PshBI	ATTAAT	1 cut(s)	1470
Psp124BI	GAGCTC	1 cut(s)	108
Psp1406I	AACGTT	1 cut(s)	608
Psp6I	CCWGG	3 cut(s)	328, 1264, 1477
PspFI	CCCAGC	1 cut(s)	1223
PspGI	CCWGG	3 cut(s)	328, 1264, 1477
PspN4I	GGNNCC	9 cut(s)	135, 136, 299, 459, 547, 657, 909, 1262, 1298
PspOMI	GGGCCC	1 cut(s)	133
PstI	CTGCAG	1 cut(s)	778
PstNI	CAGNNNCTG	1 cut(s)	1316
PsuI	RGATCY	1 cut(s)	1423
PsyI	GACNNNGTC	1 cut(s)	314
PvuII	CAGCTG	1 cut(s)	156
RsaI	GTAC	3 cut(s)	581, 1100, 1262
RsaNI	GTAC	3 cut(s)	580, 1099, 1261
RseI	CAYNNNNRTG	1 cut(s)	1238
SacI	GAGCTC	1 cut(s)	108
SapI	GCTCTTC	1 cut(s)	222
SaqAI	TTAA	5 cut(s)	669, 1136, 1332, 1470, 1552
SatI	GCNGC	8 cut(s)	73, 121, 531, 534, 627, 687, 1179, 1383
Sau3AI	GATC	6 cut(s)	1062, 1117, 1156, 1268, 1290, 1423
SchI	GAGTC	2 cut(s)	1050, 1160
ScrFI	CCNGG	6 cut(s)	330, 339, 355, 1266, 1445, 1479
SduI	GDGCHC	4 cut(s)	108, 137, 273, 1516
SfaNI	GCATC	3 cut(s)	349, 724, 733
SfcI	CTRYAG	1 cut(s)	774
SinI	GGWCC	5 cut(s)	19, 297, 449, 546, 877
SmiMI	CAYNNNNRTG	1 cut(s)	1238
SmlI	CTYRAG	2 cut(s)	41, 491
SmoI	CTYRAG	2 cut(s)	41, 491
Sse9I	AATT	3 cut(s)	1372, 1467, 1471
SsiI	CCGC	8 cut(s)	70, 112, 121, 232, 295, 737, 1346, 1492
SspMI	CTAG	1 cut(s)	752
SstI	GAGCTC	1 cut(s)	108
StyD4I	CCNGG	6 cut(s)	328, 337, 353, 1264, 1443, 1477
StyI	CCWWGG	4 cut(s)	129, 1233, 1399, 1413
TaaI	ACNGT	3 cut(s)	214, 1003, 1536
TaiI	ACGT	1 cut(s)	611
TaqI	TCGA	2 cut(s)	381, 1065
TasI	AATT	3 cut(s)	1372, 1467, 1471
TatI	WGTACW	2 cut(s)	579, 1098
TauI	GCSGC	1 cut(s)	123
TfiI	GAWTC	3 cut(s)	501, 866, 1361
Tru1I	TTAA	5 cut(s)	669, 1136, 1332, 1470, 1552
Tru9I	TTAA	5 cut(s)	669, 1136, 1332, 1470, 1552
TscAI	CASTG	2 cut(s)	1008, 1362
TseFI	GTSAC	3 cut(s)	308, 1405, 1540
TseI	GCWGC	7 cut(s)	72, 530, 533, 626, 686, 1178, 1382
Tsp45I	GTSAC	3 cut(s)	308, 1405, 1540
TspDTI	ATGAA	7 cut(s)	510, 547, 575, 995, 1320, 1331, 1366
TspRI	CASTG	2 cut(s)	1008, 1362
Tth111I	GACNNNGTC	1 cut(s)	314
Van91I	CCANNNNNTGG	1 cut(s)	917
VpaK11BI	GGWCC	5 cut(s)	19, 297, 449, 546, 877
VspI	ATTAAT	1 cut(s)	1470
XceI	RCATGY	1 cut(s)	1015
XcmI	CCANNNNNNNNNTGG	1 cut(s)	887
XmnI	GAANNNNTTC	1 cut(s)	86
XspI	CTAG	1 cut(s)	752

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.