MD09G1022100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0028464
Physical Location & Seq
Forward (+)
1335224 .. 1336049
826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1022100.v1.1.491

Sequence Viewer

Length: 462 bp
ATGACATTAAAGTTCATCCCTAATCCGATCCCCGGATTACTTGATGAACACGGTTGCATCAAGTATCAAAACGACGTTGCTAGACAGTTCAACAAGGTTCTCAACCAACAAGTGATCCAGCTAAGGACGGAGCTCCCCCTTTCTGCTATAACATATGTCGATGTCTTTTCCGCAAAGTATCAACTCTTCGGTAGAGCAAAGGAGCACGGATTTGGGAATACTGCAAACATCTGCCGTGGATATTTTGAAGATAAGAGCCAAGTGTGGTGTGGGCAAAGGGCAAACGTAAATTGGACAGAAGTGTTTGGTGGTTCATGTGAAGATCCTTCCTCGTATGTGAGCTGGGATGGTGTGCACTACACTGATGCTGCAAATCATTTGATTGCTGCTCAAATAATTAACGGTTCATATTCAGACCCACCAGTTCCAATCAAAAGATCATGTCACACGATTCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

154

Amino Acids

17.15

Weight (kDa)

6.49

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 11 - 132 9.6e-11 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028464)

Species Orthologous Gene IDs
malus_domestica MD09G1022100.v1.1
pyrus_communis pycom111g01850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 171
AclWI GGATC 3 cut(s) 22, 109, 317
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 3 cut(s) 91, 248, 455
AluBI AGCT 3 cut(s) 121, 133, 342
AluI AGCT 3 cut(s) 121, 133, 342
Alw21I GWGCWC 3 cut(s) 135, 207, 357
Alw44I GTGCAC 1 cut(s) 353
AlwI GGATC 3 cut(s) 22, 109, 317
ApaLI GTGCAC 1 cut(s) 353
ApeKI GCWGC 2 cut(s) 368, 386
AsuC2I CCSGG 1 cut(s) 33
BaeGI GKGCMC 1 cut(s) 357
BanII GRGCYC 1 cut(s) 135
BarI GAAGNNNNNNTAC 2 cut(s) 170, 202
Bbv12I GWGCWC 3 cut(s) 135, 207, 357
BbvI GCAGC 2 cut(s) 355, 373
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 219
BcnI CCSGG 1 cut(s) 33
BfaI CTAG 1 cut(s) 81
BisI GCNGC 2 cut(s) 369, 387
BlsI GCNGC 2 cut(s) 370, 388
Bme1390I CCNGG 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 33
BmsI GCATC 2 cut(s) 66, 355
Bpu10I CCTNAGC 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 31, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 31, 235
BseGI GGATG 2 cut(s) 15, 352
BseLI CCNNNNNNNGG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 422
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 2 cut(s) 355, 373
BseYI CCCAGC 1 cut(s) 342
BsiHKAI GWGCWC 3 cut(s) 135, 207, 357
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 32
Bsp1286I GDGCHC 3 cut(s) 135, 207, 357
Bsp143I GATC 4 cut(s) 27, 114, 322, 437
BspACI CCGC 1 cut(s) 171
BspPI GGATC 3 cut(s) 22, 109, 317
BsrI ACTGG 1 cut(s) 422
BssECI CCNNGG 2 cut(s) 31, 235
BssMI GATC 4 cut(s) 27, 114, 322, 437
Bst4CI ACNGT 3 cut(s) 53, 87, 404
Bst6I CTCTTC 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 2 cut(s) 15, 352
BstKTI GATC 4 cut(s) 30, 117, 325, 440
BstMBI GATC 4 cut(s) 27, 114, 322, 437
BstSCI CCNGG 1 cut(s) 31
BstSLI GKGCMC 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 355, 373
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 2 cut(s) 15, 352
BtsIMutI CAGTG 1 cut(s) 360
CviAII CATG 3 cut(s) 315, 441, 459
CviJI RGCY 4 cut(s) 121, 133, 258, 342
CviKI_1 RGCY 4 cut(s) 121, 133, 258, 342
DdeI CTNAG 1 cut(s) 122
DpnI GATC 4 cut(s) 29, 116, 324, 439
DpnII GATC 4 cut(s) 27, 114, 322, 437
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 3 cut(s) 318, 444, 462
FaiI YATR 8 cut(s) 149, 154, 156, 316, 336, 409, 442, 460
FatI CATG 3 cut(s) 314, 440, 458
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 2 cut(s) 369, 387
FokI GGATG 2 cut(s) 2, 359
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 2 cut(s) 369, 387
FspBI CTAG 1 cut(s) 81
GluI GCNGC 2 cut(s) 369, 387
GsaI CCCAGC 1 cut(s) 346
HapII CCGG 1 cut(s) 33
Hin1II CATG 3 cut(s) 318, 444, 462
HinfI GANTC 1 cut(s) 451
HpaII CCGG 1 cut(s) 33
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 2 cut(s) 27, 415
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 336
HpyCH4III ACNGT 3 cut(s) 53, 87, 404
HpyCH4IV ACGT 2 cut(s) 75, 285
HpyCH4V TGCA 4 cut(s) 57, 224, 355, 371
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 2 cut(s) 75, 285
Hsp92II CATG 3 cut(s) 318, 444, 462
Kzo9I GATC 4 cut(s) 27, 114, 322, 437
LmnI GCTCC 3 cut(s) 130, 138, 202
LpnPI CCDG 4 cut(s) 46, 131, 328, 435
Lsp1109I GCAGC 2 cut(s) 355, 373
LweI GCATC 2 cut(s) 66, 355
MaeI CTAG 1 cut(s) 81
MaeII ACGT 2 cut(s) 75, 285
MaeIII GTNAC 1 cut(s) 443
MalI GATC 4 cut(s) 29, 116, 324, 439
MboI GATC 4 cut(s) 27, 114, 322, 437
MboII GAAGA 3 cut(s) 178, 260, 332
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 3 cut(s) 135, 207, 357
MluCI AATT 2 cut(s) 289, 396
MnlI CCTC 1 cut(s) 340
MseI TTAA 2 cut(s) 8, 399
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 33
NciI CCSGG 1 cut(s) 33
NdeI CATATG 1 cut(s) 154
NdeII GATC 4 cut(s) 27, 114, 322, 437
NlaIII CATG 3 cut(s) 318, 444, 462
NmuCI GTSAC 1 cut(s) 443
PfeI GAWTC 1 cut(s) 451
PkrI GCNGC 2 cut(s) 370, 388
Psp124BI GAGCTC 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 342
PsuI RGATCY 1 cut(s) 322
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 8, 399
SatI GCNGC 2 cut(s) 369, 387
Sau3AI GATC 4 cut(s) 27, 114, 322, 437
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 3 cut(s) 135, 207, 357
SetI ASST 6 cut(s) 78, 99, 123, 135, 288, 344
SfaNI GCATC 2 cut(s) 66, 355
Sse9I AATT 2 cut(s) 289, 396
SsiI CCGC 1 cut(s) 171
SspMI CTAG 1 cut(s) 81
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 31
TaaI ACNGT 3 cut(s) 53, 87, 404
TaiI ACGT 2 cut(s) 78, 288
TaqI TCGA 1 cut(s) 159
TasI AATT 2 cut(s) 289, 396
TfiI GAWTC 1 cut(s) 451
Tru1I TTAA 2 cut(s) 8, 399
Tru9I TTAA 2 cut(s) 8, 399
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 2 cut(s) 368, 386
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 4 cut(s) 4, 60, 303, 396
TspGWI ACGGA 2 cut(s) 143, 222
TspRI CASTG 1 cut(s) 367
VneI GTGCAC 1 cut(s) 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.