MD09G1037300.v1.1

Protein ALTERED XYLOGLUCAN 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0028473
Physical Location & Seq
Reverse (-)
2275374 .. 2276643
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1037300.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCTGTTATCGGCGACTCTGTTGCGCAGAACCATTTTGAGTTTCTGCTCTGCCTCTTGTCACAGGAGGAATCCCCTAAAGACATTTACAAGGATTTCGAAGATCACTTCACGACATGGTACTTTCCACAACATGACTTCACCCTTATGAAATTATGGTCCAAATTTCTCATTGCTGCAGAAGAGAGGATGGTTAATGGTAAAGGATCCAGCATTTTCGATTTGCAACTAGACAAAGTCGACGAGAAATGGGCAGAACACCTCCCGGATTTAGACTACGCTATATTCTCAGCAGGGCATTGGTTTAACCGGATTATGTACCTGCACCAAGGTGGCAACCTCATTGGATGCTTCTACTGCGGTGAACCTAATGTCACCAATTACAACGGTAGCCACATCGTTCACTTGGCGTTTCGAACTGCATTCAAGTATATCATGGATTGCAAGTACTGCAAGGGAGGCCTCCTGACCTTGTTGAGGACATTTACACCGGCACATTTTGAGGGTGGCGTTTGGAACACCAGTGGGTACTGCAATCAAATGAGTCCTTTGAGCGAGGCAGACATTGATTTGGGACGTTCGGATTGGGAACTGAGGAGCATTCAGGTTGAGGAAATTGAAAGAGCAAGAAAAGAAGGGGAAGTGCAGGGAAAGACGTTTAGGGTTTTGGATGTAACCAGGGCCATGTTTATGAGCCGGACGGGCACCTCGGAGCTCACTGGAACAAGTGGATGGACGGTTACAACGATTGCGTGCATTGGTGCATGCCAGGCCCTATTGATTACTGGAACCATTTTCTGA

Protein Analysis

267

Amino Acids

30.25

Weight (kDa)

5.35

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 1 - 235 2.7e-51 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028473)

Species Orthologous Gene IDs
malus_domestica MD09G1037300.v1.1
pyrus_communis pycom111g02990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 330
AccB1I GGYRCC 1 cut(s) 704
AccI GTMKAC 1 cut(s) 240
AciI CCGC 1 cut(s) 360
AclWI GGATC 2 cut(s) 201, 214
AcsI RAATTY 1 cut(s) 164
AfaI GTAC 4 cut(s) 122, 320, 449, 530
AfiI CCNNNNNNNGG 1 cut(s) 477
AgsI TTSAA 2 cut(s) 427, 620
AjnI CCWGG 2 cut(s) 677, 768
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 1 cut(s) 715
AluI AGCT 1 cut(s) 715
Alw21I GWGCWC 1 cut(s) 717
AlwI GGATC 2 cut(s) 201, 214
AoxI GGCC 3 cut(s) 460, 681, 771
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 164
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 3 cut(s) 159, 681, 772
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 133, 367, 374
AsuII TTCGAA 2 cut(s) 99, 415
AvaII GGWCC 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 707
BamHI GGATCC 1 cut(s) 206
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 1 cut(s) 163
BccI CCATC 2 cut(s) 184, 726
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 679, 770
BcnI CCSGG 1 cut(s) 266
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 177
BfuAI ACCTGC 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 702
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 3 cut(s) 266, 679, 770
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 159, 681, 772
BmiI GGNNCC 3 cut(s) 208, 706, 790
BmrFI CCNGG 3 cut(s) 266, 679, 770
BmsI GCATC 1 cut(s) 338
Bpu14I TTCGAA 2 cut(s) 99, 415
BpuMI CCSGG 1 cut(s) 266
BsaJI CCNNGG 3 cut(s) 328, 678, 708
BsaWI WCCGGW 1 cut(s) 309
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 3 cut(s) 522, 724, 790
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 2 cut(s) 679, 770
BseDI CCNNGG 3 cut(s) 328, 678, 708
BseGI GGATG 4 cut(s) 195, 353, 676, 737
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 2 cut(s) 303, 584
BseNI ACTGG 3 cut(s) 522, 724, 790
BseRI GAGGAG 1 cut(s) 610
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 1 cut(s) 163
BsgI GTGCAG 2 cut(s) 308, 665
BshFI GGCC 3 cut(s) 462, 683, 773
BshNI GGYRCC 1 cut(s) 704
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 4 cut(s) 266, 310, 491, 697
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 1 cut(s) 477
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 2 cut(s) 422, 600
BsnI GGCC 3 cut(s) 462, 683, 773
Bsp119I TTCGAA 2 cut(s) 99, 415
Bsp1286I GDGCHC 2 cut(s) 707, 717
Bsp143I GATC 2 cut(s) 103, 206
BspACI CCGC 1 cut(s) 360
BspANI GGCC 3 cut(s) 462, 683, 773
BspCNI CTCAG 2 cut(s) 302, 585
BspLI GGNNCC 3 cut(s) 208, 706, 790
BspMAI CTGCAG 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 330
BspPI GGATC 2 cut(s) 201, 214
BspT104I TTCGAA 2 cut(s) 99, 415
BspT107I GGYRCC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 490
BsrI ACTGG 3 cut(s) 522, 724, 790
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 328, 678, 708
BssMI GATC 2 cut(s) 103, 206
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 2 cut(s) 679, 770
Bst4CI ACNGT 2 cut(s) 389, 739
Bst6I CTCTTC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 450
BstBI TTCGAA 2 cut(s) 99, 415
BstC8I GCNNGC 2 cut(s) 754, 766
BstDEI CTNAG 2 cut(s) 289, 593
BstENI CCTNNNNNAGG 1 cut(s) 475
BstF5I GGATG 4 cut(s) 195, 353, 676, 737
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 2 cut(s) 106, 209
BstMBI GATC 2 cut(s) 103, 206
BstMWI GCNNNNNNNGC 5 cut(s) 357, 450, 459, 702, 770
BstNI CCWGG 2 cut(s) 679, 770
BstNSI RCATGY 1 cut(s) 768
BstSCI CCNGG 3 cut(s) 264, 677, 768
BstSFI CTRYAG 1 cut(s) 177
BstSLI GKGCMC 1 cut(s) 707
BstV1I GCAGC 1 cut(s) 163
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 3 cut(s) 462, 683, 773
BtsCI GGATG 4 cut(s) 195, 353, 676, 737
BtsIMutI CAGTG 2 cut(s) 529, 717
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 2 cut(s) 754, 766
CfoI GCGC 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 3 cut(s) 159, 681, 772
Csp6I GTAC 4 cut(s) 121, 319, 448, 529
CviAII CATG 5 cut(s) 117, 134, 436, 685, 765
CviJI RGCY 7 cut(s) 5, 393, 462, 683, 696, 715, 773
CviKI_1 RGCY 7 cut(s) 5, 393, 462, 683, 696, 715, 773
CviQI GTAC 4 cut(s) 121, 319, 448, 529
DdeI CTNAG 2 cut(s) 289, 593
DpnI GATC 2 cut(s) 105, 208
DpnII GATC 2 cut(s) 103, 206
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 715
Eco130I CCWWGG 1 cut(s) 328
Eco147I AGGCCT 1 cut(s) 462
Eco24I GRGCYC 1 cut(s) 717
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 715
EcoICRI GAGCTC 1 cut(s) 715
EcoNI CCTNNNNNAGG 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 772
EcoRII CCWGG 2 cut(s) 677, 768
EcoT14I CCWWGG 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 5 cut(s) 120, 137, 439, 688, 768
FaqI GGGAC 1 cut(s) 588
FatI CATG 5 cut(s) 116, 133, 435, 684, 764
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 4 cut(s) 202, 360, 683, 744
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 230
FspI TGCGCA 1 cut(s) 27
GlaI GCGC 1 cut(s) 27
GluI GCNGC 1 cut(s) 177
HaeIII GGCC 3 cut(s) 462, 683, 773
HapII CCGG 4 cut(s) 266, 310, 491, 697
HhaI GCGC 1 cut(s) 28
Hin1II CATG 5 cut(s) 120, 137, 439, 688, 768
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 3 cut(s) 17, 71, 544
HpaII CCGG 4 cut(s) 266, 310, 491, 697
HphI GGTGA 3 cut(s) 133, 367, 374
Hpy166II GTNNAC 3 cut(s) 241, 365, 403
Hpy188I TCNGA 3 cut(s) 583, 712, 800
Hpy188III TCNNGA 2 cut(s) 112, 466
Hpy8I GTNNAC 3 cut(s) 241, 365, 403
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 629
HpyCH4III ACNGT 2 cut(s) 389, 739
HpyCH4IV ACGT 2 cut(s) 577, 656
HpyF10VI GCNNNNNNNGC 5 cut(s) 357, 450, 459, 702, 770
HpyF3I CTNAG 2 cut(s) 289, 593
HpySE526I ACGT 2 cut(s) 577, 656
Hsp92II CATG 5 cut(s) 120, 137, 439, 688, 768
HspAI GCGC 1 cut(s) 26
Kzo9I GATC 2 cut(s) 103, 206
LmnI GCTCC 2 cut(s) 597, 712
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 230
MaeII ACGT 2 cut(s) 577, 656
MaeIII GTNAC 4 cut(s) 60, 373, 673, 739
MalI GATC 2 cut(s) 105, 208
MboI GATC 2 cut(s) 103, 206
MboII GAAGA 2 cut(s) 113, 194
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 2 cut(s) 707, 717
MluCI AATT 4 cut(s) 152, 164, 379, 615
MlyI GAGTC 2 cut(s) 11, 553
MseI TTAA 2 cut(s) 195, 306
MslI CAYNNNNRTG 3 cut(s) 146, 330, 689
MspI CCGG 4 cut(s) 266, 310, 491, 697
MspR9I CCNGG 3 cut(s) 266, 679, 770
Mva1269I GAATGC 2 cut(s) 422, 600
MvaI CCWGG 2 cut(s) 679, 770
MwoI GCNNNNNNNGC 5 cut(s) 357, 450, 459, 702, 770
NciI CCSGG 1 cut(s) 266
NdeII GATC 2 cut(s) 103, 206
NlaIII CATG 5 cut(s) 120, 137, 439, 688, 768
NlaIV GGNNCC 3 cut(s) 208, 706, 790
NmuCI GTSAC 2 cut(s) 60, 373
NsbI TGCGCA 1 cut(s) 27
NspI RCATGY 1 cut(s) 768
NspV TTCGAA 2 cut(s) 99, 415
OliI CACNNNNGTG 1 cut(s) 330
PaeI GCATGC 1 cut(s) 768
PceI AGGCCT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 743
PctI GAATGC 2 cut(s) 422, 600
PfeI GAWTC 1 cut(s) 71
PflFI GACNNNGTC 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 2 cut(s) 11, 552
PpsI GAGTC 2 cut(s) 11, 552
Psp124BI GAGCTC 1 cut(s) 717
Psp6I CCWGG 2 cut(s) 677, 768
PspGI CCWGG 2 cut(s) 677, 768
PspN4I GGNNCC 3 cut(s) 208, 706, 790
PspPI GGNCC 3 cut(s) 159, 681, 772
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 206
PsyI GACNNNGTC 1 cut(s) 236
RsaI GTAC 4 cut(s) 122, 320, 449, 530
RsaNI GTAC 4 cut(s) 121, 319, 448, 529
RseI CAYNNNNRTG 3 cut(s) 146, 330, 689
SacI GAGCTC 1 cut(s) 717
SalI GTCGAC 1 cut(s) 239
SaqAI TTAA 2 cut(s) 195, 306
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 103, 206
Sau96I GGNCC 3 cut(s) 159, 681, 772
ScaI AGTACT 1 cut(s) 449
SchI GAGTC 2 cut(s) 11, 553
ScrFI CCNGG 3 cut(s) 266, 679, 770
SduI GDGCHC 2 cut(s) 707, 717
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 177
SfuI TTCGAA 2 cut(s) 99, 415
SinI GGWCC 1 cut(s) 159
SmiMI CAYNNNNRTG 3 cut(s) 146, 330, 689
SphI GCATGC 1 cut(s) 768
Sse9I AATT 4 cut(s) 152, 164, 379, 615
SseBI AGGCCT 1 cut(s) 462
SsiI CCGC 1 cut(s) 360
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 717
StuI AGGCCT 1 cut(s) 462
StyD4I CCNGG 3 cut(s) 264, 677, 768
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 2 cut(s) 389, 739
TaiI ACGT 2 cut(s) 580, 659
TaqI TCGA 4 cut(s) 99, 219, 240, 415
TasI AATT 4 cut(s) 152, 164, 379, 615
TatI WGTACW 1 cut(s) 447
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 2 cut(s) 195, 306
Tru9I TTAA 2 cut(s) 195, 306
TscAI CASTG 2 cut(s) 529, 724
TseFI GTSAC 2 cut(s) 60, 373
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 2 cut(s) 60, 373
TspDTI ATGAA 1 cut(s) 164
TspRI CASTG 2 cut(s) 529, 724
Tth111I GACNNNGTC 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 159
XagI CCTNNNNNAGG 1 cut(s) 475
XapI RAATTY 1 cut(s) 164
XceI RCATGY 1 cut(s) 768
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 1 cut(s) 230
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.