MD09G1144000.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0028436
Physical Location & Seq
Forward (+)
11204442 .. 11206589
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1144000.v1.1.491

Sequence Viewer

Length: 258 bp
ATGAGTGATCCGAGCGGAAACTATGCCGGCTGGAAAGCTGCTGTGATTGGGGCTAACAACCAAGCTGCGCAGTCAATGCTTAAGCAGGATTACAAGGATGATATGACTAGGGAAGAGGGAGTCGAGCTAGCGCTGAAGGTGCTCAGTAAAACCATGGACAGCACAAGTCTCACCCCAGAGAAGCTTGAATTGGCTGAGGTGTTGCTATTGATGTTTTGGATCTCGAGGGTTCGATTTGGTTCGGTGTTTCAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019773 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome Proteasome_A_N

Protein Analysis

86

Amino Acids

9.47

Weight (kDa)

4.96

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 2 - 67 8.8e-16 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028436)

Species Orthologous Gene IDs
malus_domestica MD09G1144000.v1.1
pyrus_communis pycom09g06340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 69
AccBSI CCGCTC 1 cut(s) 15
AciI CCGC 1 cut(s) 15
AclWI GGATC 2 cut(s) 2, 227
AcuI CTGAAG 1 cut(s) 155
AfeI AGCGCT 1 cut(s) 132
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 1 cut(s) 188
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 4 cut(s) 38, 65, 127, 184
AluI AGCT 4 cut(s) 38, 65, 127, 184
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 2 cut(s) 2, 227
Ama87I CYCGRG 1 cut(s) 223
Aor51HI AGCGCT 1 cut(s) 132
ApeKI GCWGC 2 cut(s) 38, 65
AspLEI GCGC 2 cut(s) 70, 133
AsuHPI GGTGA 1 cut(s) 163
AsuNHI GCTAGC 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 25, 52
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 2 cut(s) 108, 128
BfoI RGCGCY 1 cut(s) 134
BfrI CTTAAG 1 cut(s) 80
BisI GCNGC 2 cut(s) 39, 66
BlsI GCNGC 2 cut(s) 40, 67
BmeT110I CYCGRG 1 cut(s) 223
BmtI GCTAGC 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 153
Bse118I RCCGGY 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 103
BseMII CTCAG 2 cut(s) 157, 186
BseXI GCAGC 2 cut(s) 25, 52
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 27
BsmAI GTCTC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 7, 219
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 1 cut(s) 15
BspCNI CTCAG 2 cut(s) 156, 187
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 2 cut(s) 2, 227
BspTI CTTAAG 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 15
BsrFI RCCGGY 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 7, 219
BssT1I CCWWGG 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 108
BstAFI CTTAAG 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 28, 129
BstDEI CTNAG 2 cut(s) 143, 195
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 103
BstH2I RGCGCY 1 cut(s) 134
BstHHI GCGC 2 cut(s) 70, 133
BstKTI GATC 2 cut(s) 10, 222
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 2 cut(s) 7, 219
BstMWI GCNNNNNNNGC 2 cut(s) 76, 139
BstV1I GCAGC 2 cut(s) 25, 52
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 103
Cac8I GCNNGC 2 cut(s) 28, 129
CfoI GCGC 2 cut(s) 70, 133
Cfr10I RCCGGY 1 cut(s) 26
CviAII CATG 1 cut(s) 154
CviJI RGCY 7 cut(s) 30, 38, 53, 65, 127, 184, 194
CviKI_1 RGCY 7 cut(s) 30, 38, 53, 65, 127, 184, 194
DdeI CTNAG 2 cut(s) 143, 195
DpnI GATC 2 cut(s) 9, 221
DpnII GATC 2 cut(s) 7, 219
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 153
Eco47III AGCGCT 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 1 cut(s) 157
FaiI YATR 3 cut(s) 24, 104, 155
FatI CATG 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 39, 66
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 2 cut(s) 39, 66
FspBI CTAG 2 cut(s) 108, 128
FspI TGCGCA 1 cut(s) 69
GlaI GCGC 2 cut(s) 69, 132
GluI GCNGC 2 cut(s) 39, 66
HaeII RGCGCY 1 cut(s) 134
HapII CCGG 1 cut(s) 27
HhaI GCGC 2 cut(s) 70, 133
Hin1II CATG 1 cut(s) 157
Hin6I GCGC 2 cut(s) 68, 131
HinP1I GCGC 2 cut(s) 68, 131
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 1 cut(s) 120
HpaII CCGG 1 cut(s) 27
HphI GGTGA 1 cut(s) 163
Hpy188I TCNGA 2 cut(s) 12, 252
Hpy188III TCNNGA 1 cut(s) 223
HpyAV CCTTC 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 139
HpyF3I CTNAG 2 cut(s) 143, 195
Hsp92II CATG 1 cut(s) 157
HspAI GCGC 2 cut(s) 68, 131
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Kzo9I GATC 2 cut(s) 7, 219
LpnPI CCDG 4 cut(s) 16, 40, 71, 189
Lsp1109I GCAGC 2 cut(s) 25, 52
MaeI CTAG 2 cut(s) 108, 128
MalI GATC 2 cut(s) 9, 221
MbiI CCGCTC 1 cut(s) 15
MboI GATC 2 cut(s) 7, 219
MboII GAAGA 1 cut(s) 125
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 2 cut(s) 188, 253
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 3 cut(s) 109, 190, 219
MroNI GCCGGC 1 cut(s) 26
MseI TTAA 2 cut(s) 81, 256
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 76, 139
NaeI GCCGGC 1 cut(s) 28
NcoI CCATGG 1 cut(s) 153
NdeII GATC 2 cut(s) 7, 219
NgoMIV GCCGGC 1 cut(s) 26
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 1 cut(s) 157
NsbI TGCGCA 1 cut(s) 69
PaeR7I CTCGAG 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 28
PkrI GCNGC 2 cut(s) 40, 67
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PsuI RGATCY 1 cut(s) 219
SaqAI TTAA 2 cut(s) 81, 256
SatI GCNGC 2 cut(s) 39, 66
Sau3AI GATC 2 cut(s) 7, 219
SchI GAGTC 1 cut(s) 129
SduI GDGCHC 1 cut(s) 144
SetI ASST 6 cut(s) 40, 67, 129, 141, 186, 201
Sfr274I CTCGAG 1 cut(s) 223
SlaI CTCGAG 1 cut(s) 223
SmlI CTYRAG 2 cut(s) 80, 223
SmoI CTYRAG 2 cut(s) 80, 223
Sse9I AATT 2 cut(s) 188, 253
SsiI CCGC 1 cut(s) 15
SspMI CTAG 2 cut(s) 108, 128
StyI CCWWGG 1 cut(s) 153
TaqI TCGA 3 cut(s) 123, 224, 232
TasI AATT 2 cut(s) 188, 253
Tru1I TTAA 2 cut(s) 81, 256
Tru9I TTAA 2 cut(s) 81, 256
TseI GCWGC 2 cut(s) 38, 65
Vha464I CTTAAG 1 cut(s) 80
XhoI CTCGAG 1 cut(s) 223
XspI CTAG 2 cut(s) 108, 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.