MD10G1248400.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0028220
Physical Location & Seq
Forward (+)
34136665 .. 34138362
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1248400.v1.1.491

Sequence Viewer

Length: 1698 bp
ATGGGTGGTACTGCCTCCACATCCAAGGTAACGGGTGTTAGCGAACAAAACGATCCGCTATGGCTTTGTGGACAGAGAAAAGACGCGCTGGAATTGGCCATAAAGAGAAGGTCTCTGTTTGTGGATGCATTATGCAAGTATAACCGATCGCTTTATGAAGTAGCAATCGCTCTAAGGATTGCAAACGTCCATTCTCAAACCAAAGATTGCTCGTCTTCGGATTCCATAAGTTCGATGAGCTCTGCAAAGGTAAATAGAGAGAAAGTAGGAGAAGAAACCCGACATCTCAAAGAAGAAGAAGGCAATGTTGAGACTCAAAGGCGTTCTGGTGATGAGAGTAGTGAAAAAAGAGAGGGAAATTTGAGTGATTATGATGTGGGTCAAGGGAGGGAATTGGTGGAAGCATTGAAAGATGTTGAGGAATATTTCATTAAAGCCTATGAATCTGGTTTGGAGGTTTCTAAGATGCTTGATGCCAATATGGATCAAATACAATCTTCTGTGGAGGAAATTGAAGAAAAATCAAACAAGCTCGAGCGTTCATTGACGACACGAAACCACCCAAACAAGCTTATGCGTTCACTTCCACTAAAACGCTCCACCTCATATAGATGTTCCTCCAAAAGCGTCCACAAATCAAGCTCCAGAGCTTCTTCAAAATATTATTTCAACAGTGGTATTTCTGAAGGCAATGGAGCAAAGACATTCATGGGCCATTCGTTCACGCTGAGGGAACTGCATGAGCAAGTTAAAATACTTCATAAAACGATGATGGCTACAGAGGAATCCAGGAAAGAATTGAAGGAATTGCAAAAGGAAAAGAAAAGAGAAGGTGTGCTCAAGGGTACTAGGGATAGTACAGGAGCTGGAATTGCGAAGTTGGAGGCCAGAATCAAGGTTTTTGAAAAAGAAGAAGAAGTACAGTACTCTGAAATTCATAGGCTAACAGATAAAGAGATGAAGCCACAACTTGTTGCCTTGTTTCACTGCCTAATGAGAAATATGAGAATCGTGTCGGAATATCATGATGCCCAAACCAGAATCATGAAGACAGTAAAAGTGCCCGACGGAGTGTTCTGCAGTGACTCACACCAGCTTACTACCTACTCTAAGCTTGAAGCAGAAATTCCAAAATGGTGTGCTTGCTTTACATCATATGTCTCTTCATTGAAGGCCTATGTTGAATCTCTTAATGACGGGCTATTCAAGTTTGCTGCTGCTAACAACGACAAGCCTTCGCTATATCATCATTGGTTAGTTTGTTTGAATAAACTTCCATATAAAGATGTGACGTCTGCCATGACAGAGTTTGGGAATGATGTCCGAGAGCTGATGATTCAACAAGGCGAGGAGCATAAGCTACAGAAAACGGTTGACAAACTTTCCAGAAAAATACATGAAAACTCAAAGAAGGAGCATTTGAAAAAGAAGGTAGATGAAGGGAAGAAGGAGCAGTTGATTGAGGAGAAGAGGCAAGTGGAAAAGAAGCTAAAGGCAGAAAAGTTGAAAACGCGTCATTTCGCTGAGAGTGGATTTCAGGAAGGATTCTCCAAGGTTTTCGAATACCTGGCTAATTATTCAAGTGGTGCTACGGAAATGTATGATGGCCTTATAGCATTCGACGGAGATGCATGCGTGGGGATTGATGACGAAAGTAACAGCAACTCATCAGAACATGTTACAGGCAATTTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

566

Amino Acids

64.22

Weight (kDa)

7.59

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 6 - 59 4.5e-08 Protein of unknown function (DUF630)
DUF632 PF04782 132 - 442 7.3e-42 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028220)

Species Orthologous Gene IDs
malus_domestica MD10G1248400.v1.1
pyrus_communis pycom10g20650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1295
AccII CGCG 2 cut(s) 86, 1513
AciI CCGC 1 cut(s) 56
AclWI GGATC 2 cut(s) 47, 492
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 3 cut(s) 358, 933, 1125
AcuI CTGAAG 1 cut(s) 705
AcyI GRCGYC 1 cut(s) 1292
AfaI GTAC 5 cut(s) 10, 847, 859, 921, 926
AflIII ACRYGT 2 cut(s) 1511, 1675
AjnI CCWGG 2 cut(s) 788, 1566
Alw21I GWGCWC 2 cut(s) 242, 840
Alw26I GTCTC 3 cut(s) 117, 305, 1165
AlwI GGATC 2 cut(s) 47, 492
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 5 cut(s) 96, 712, 885, 1173, 1606
ApeKI GCWGC 2 cut(s) 1214, 1217
ApoI RAATTY 3 cut(s) 358, 933, 1125
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 712
AsuHPI GGTGA 1 cut(s) 341
AsuII TTCGAA 1 cut(s) 1560
AvaI CYCGRG 1 cut(s) 533
BaeGI GKGCMC 1 cut(s) 1065
BalI TGGCCA 1 cut(s) 98
BanII GRGCYC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 903, 935
BbsI GAAGAC 2 cut(s) 207, 1055
Bbv12I GWGCWC 2 cut(s) 242, 840
BbvCI CCTCAGC 1 cut(s) 728
BbvI GCAGC 2 cut(s) 1201, 1204
BccI CCATC 2 cut(s) 766, 1598
BcgI CGANNNNNNTGC 2 cut(s) 1610, 1644
BciT130I CCWGG 2 cut(s) 790, 1568
BcoDI GTCTC 3 cut(s) 117, 305, 1165
BfaI CTAG 1 cut(s) 849
BfmI CTRYAG 3 cut(s) 777, 1078, 1361
BisI GCNGC 2 cut(s) 1215, 1218
BlsI GCNGC 2 cut(s) 1216, 1219
BmcAI AGTACT 1 cut(s) 926
Bme1390I CCNGG 2 cut(s) 790, 1568
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 712
BmrFI CCNGG 2 cut(s) 790, 1568
BmsI GCATC 5 cut(s) 115, 456, 463, 1018, 1618
BpiI GAAGAC 2 cut(s) 207, 1055
BplI GAGNNNNNCTC 2 cut(s) 97, 129
BpmI CTGGAG 1 cut(s) 628
Bpu10I CCTNAGC 1 cut(s) 728
Bpu14I TTCGAA 1 cut(s) 1560
BpuEI CTTGAG 1 cut(s) 824
BsaHI GRCGYC 1 cut(s) 1292
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 24, 1551
Bse3DI GCAATG 2 cut(s) 310, 697
BseBI CCWGG 2 cut(s) 790, 1568
BseDI CCNNGG 2 cut(s) 24, 1551
BseGI GGATG 2 cut(s) 20, 130
BseMI GCAATG 2 cut(s) 310, 697
BseMII CTCAG 2 cut(s) 719, 1515
BseRI GAGGAG 2 cut(s) 1364, 1478
BseSI GKGCMC 1 cut(s) 1065
BseXI GCAGC 2 cut(s) 1201, 1204
Bsh1236I CGCG 2 cut(s) 86, 1513
Bsh1285I CGRYCG 1 cut(s) 149
BshFI GGCC 5 cut(s) 98, 714, 887, 1175, 1608
BsiEI CGRYCG 1 cut(s) 149
BsiHKAI GWGCWC 2 cut(s) 242, 840
BsiHKCI CYCGRG 1 cut(s) 533
BsmAI GTCTC 3 cut(s) 117, 305, 1165
BsmI GAATGC 1 cut(s) 1616
BsnI GGCC 5 cut(s) 98, 714, 887, 1175, 1608
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 533
Bsp119I TTCGAA 1 cut(s) 1560
Bsp1286I GDGCHC 3 cut(s) 242, 840, 1065
Bsp143I GATC 3 cut(s) 52, 146, 484
BspACI CCGC 1 cut(s) 56
BspANI GGCC 5 cut(s) 98, 714, 887, 1175, 1608
BspCNI CTCAG 2 cut(s) 720, 1516
BspFNI CGCG 2 cut(s) 86, 1513
BspHI TCATGA 2 cut(s) 1024, 1044
BspMAI CTGCAG 1 cut(s) 1082
BspPI GGATC 2 cut(s) 47, 492
BspT104I TTCGAA 1 cut(s) 1560
BspTNI GGTCTC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 310, 697
BssECI CCNNGG 2 cut(s) 24, 1551
BssMI GATC 3 cut(s) 52, 146, 484
BssNI GRCGYC 1 cut(s) 1292
BssT1I CCWWGG 2 cut(s) 24, 1551
Bst2UI CCWGG 2 cut(s) 790, 1568
Bst4CI ACNGT 4 cut(s) 674, 924, 1054, 1372
Bst6I CTCTTC 2 cut(s) 1168, 1463
BstACI GRCGYC 1 cut(s) 1292
BstBI TTCGAA 1 cut(s) 1560
BstC8I GCNNGC 2 cut(s) 1144, 1633
BstDEI CTNAG 5 cut(s) 173, 462, 728, 1110, 1524
BstF5I GGATG 2 cut(s) 20, 130
BstFNI CGCG 2 cut(s) 86, 1513
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 3 cut(s) 55, 149, 487
BstMAI GTCTC 3 cut(s) 117, 305, 1165
BstMBI GATC 3 cut(s) 52, 146, 484
BstMCI CGRYCG 1 cut(s) 149
BstMWI GCNNNNNNNGC 1 cut(s) 872
BstNI CCWGG 2 cut(s) 790, 1568
BstNSI RCATGY 2 cut(s) 1635, 1679
BstSCI CCNGG 2 cut(s) 788, 1566
BstSFI CTRYAG 3 cut(s) 777, 1078, 1361
BstSLI GKGCMC 1 cut(s) 1065
BstUI CGCG 2 cut(s) 86, 1513
BstV1I GCAGC 2 cut(s) 1201, 1204
BstV2I GAAGAC 2 cut(s) 207, 1055
BsuRI GGCC 5 cut(s) 98, 714, 887, 1175, 1608
BtsCI GGATG 2 cut(s) 20, 130
BtsI GCAGTG 2 cut(s) 985, 1087
BtsIMutI CAGTG 3 cut(s) 679, 985, 1087
Cac8I GCNNGC 2 cut(s) 1144, 1633
CaiI CAGNNNCTG 1 cut(s) 866
CciI TCATGA 2 cut(s) 1024, 1044
CfoI GCGC 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 712
CseI GACGC 3 cut(s) 92, 616, 1502
Csp6I GTAC 5 cut(s) 9, 846, 858, 920, 925
CviAII CATG 8 cut(s) 709, 740, 1025, 1045, 1300, 1397, 1632, 1676
CviQI GTAC 5 cut(s) 9, 846, 858, 920, 925
DdeI CTNAG 5 cut(s) 173, 462, 728, 1110, 1524
DpnI GATC 3 cut(s) 54, 148, 486
DpnII GATC 3 cut(s) 52, 146, 484
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 1168, 1463
EarI CTCTTC 2 cut(s) 1168, 1463
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 2 cut(s) 24, 1551
Eco147I AGGCCT 1 cut(s) 1175
Eco24I GRGCYC 1 cut(s) 242
Eco31I GGTCTC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 705
Eco88I CYCGRG 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 788, 1566
EcoT14I CCWWGG 2 cut(s) 24, 1551
EcoT22I ATGCAT 2 cut(s) 130, 1633
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 2 cut(s) 24, 1551
FaeI CATG 8 cut(s) 712, 743, 1028, 1048, 1303, 1400, 1635, 1679
FatI CATG 8 cut(s) 708, 739, 1024, 1044, 1299, 1396, 1631, 1675
FauNDI CATATG 2 cut(s) 1156, 1694
Fnu4HI GCNGC 2 cut(s) 1215, 1218
FokI GGATG 2 cut(s) 7, 137
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 1215, 1218
FspBI CTAG 1 cut(s) 849
GlaI GCGC 1 cut(s) 87
GluI GCNGC 2 cut(s) 1215, 1218
GsuI CTGGAG 1 cut(s) 628
HaeIII GGCC 5 cut(s) 98, 714, 887, 1175, 1608
HgaI GACGC 3 cut(s) 92, 616, 1502
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 1292
Hin1II CATG 8 cut(s) 712, 743, 1028, 1048, 1303, 1400, 1635, 1679
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 1375
HindII GTYRAC 1 cut(s) 1375
HindIII AAGCTT 2 cut(s) 569, 1112
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 5 cut(s) 71, 581, 631, 723, 1375
Hpy188I TCNGA 6 cut(s) 220, 685, 931, 1018, 1325, 1672
Hpy188III TCNNGA 5 cut(s) 645, 1025, 1045, 1386, 1538
Hpy8I GTNNAC 5 cut(s) 71, 581, 631, 723, 1375
Hpy99I CGWCG 2 cut(s) 1070, 1625
HpyCH4III ACNGT 4 cut(s) 674, 924, 1054, 1372
HpyCH4IV ACGT 2 cut(s) 186, 1292
HpyCH4V TGCA 8 cut(s) 128, 135, 182, 245, 739, 811, 1080, 1631
HpyF10VI GCNNNNNNNGC 1 cut(s) 872
HpyF3I CTNAG 5 cut(s) 173, 462, 728, 1110, 1524
HpySE526I ACGT 2 cut(s) 186, 1292
Hsp92I GRCGYC 1 cut(s) 1292
Hsp92II CATG 8 cut(s) 712, 743, 1028, 1048, 1303, 1400, 1635, 1679
HspAI GCGC 1 cut(s) 86
Kzo9I GATC 3 cut(s) 52, 146, 484
LmnI GCTCC 7 cut(s) 602, 647, 695, 863, 1351, 1414, 1450
Lsp1109I GCAGC 2 cut(s) 1201, 1204
LweI GCATC 5 cut(s) 115, 456, 463, 1018, 1618
MaeI CTAG 1 cut(s) 849
MaeII ACGT 2 cut(s) 186, 1292
MaeIII GTNAC 5 cut(s) 28, 1082, 1288, 1655, 1678
MalI GATC 3 cut(s) 54, 148, 486
MboI GATC 3 cut(s) 52, 146, 484
MhlI GDGCHC 3 cut(s) 242, 840, 1065
MlsI TGGCCA 1 cut(s) 98
MluI ACGCGT 1 cut(s) 1511
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 2 cut(s) 307, 1079
MmeI TCCRAC 2 cut(s) 861, 996
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 2 cut(s) 130, 1633
MscI TGGCCA 1 cut(s) 98
MseI TTAA 3 cut(s) 432, 750, 1191
MslI CAYNNNNRTG 1 cut(s) 610
Msp20I TGGCCA 1 cut(s) 98
MspR9I CCNGG 2 cut(s) 790, 1568
Mva1269I GAATGC 1 cut(s) 1616
MvaI CCWGG 2 cut(s) 790, 1568
MvnI CGCG 2 cut(s) 86, 1513
MwoI GCNNNNNNNGC 1 cut(s) 872
NdeI CATATG 2 cut(s) 1156, 1694
NdeII GATC 3 cut(s) 52, 146, 484
NlaIII CATG 8 cut(s) 712, 743, 1028, 1048, 1303, 1400, 1635, 1679
NmuCI GTSAC 2 cut(s) 1082, 1288
NsiI ATGCAT 2 cut(s) 130, 1633
NspI RCATGY 2 cut(s) 1635, 1679
NspV TTCGAA 1 cut(s) 1560
PaeI GCATGC 1 cut(s) 1635
PaeR7I CTCGAG 1 cut(s) 533
PagI TCATGA 2 cut(s) 1024, 1044
PceI AGGCCT 1 cut(s) 1175
PciI ACATGT 1 cut(s) 1675
PctI GAATGC 1 cut(s) 1616
PfeI GAWTC 9 cut(s) 221, 443, 785, 891, 1008, 1041, 1184, 1336, 1545
PfoI TCCNGGA 1 cut(s) 788
PkrI GCNGC 2 cut(s) 1216, 1219
Ple19I CGATCG 1 cut(s) 149
PleI GAGTC 2 cut(s) 307, 1079
PpsI GAGTC 2 cut(s) 307, 1079
PscI ACATGT 1 cut(s) 1675
Psp124BI GAGCTC 1 cut(s) 242
Psp6I CCWGG 2 cut(s) 788, 1566
PspGI CCWGG 2 cut(s) 788, 1566
PspPI GGNCC 1 cut(s) 712
PspXI VCTCGAGB 1 cut(s) 533
PstI CTGCAG 1 cut(s) 1082
PstNI CAGNNNCTG 1 cut(s) 866
PvuI CGATCG 1 cut(s) 149
RsaI GTAC 5 cut(s) 10, 847, 859, 921, 926
RsaNI GTAC 5 cut(s) 9, 846, 858, 920, 925
RseI CAYNNNNRTG 1 cut(s) 610
SacI GAGCTC 1 cut(s) 242
SaqAI TTAA 3 cut(s) 432, 750, 1191
SatI GCNGC 2 cut(s) 1215, 1218
Sau3AI GATC 3 cut(s) 52, 146, 484
Sau96I GGNCC 1 cut(s) 712
ScaI AGTACT 1 cut(s) 926
SchI GAGTC 2 cut(s) 307, 1079
ScrFI CCNGG 2 cut(s) 790, 1568
SduI GDGCHC 3 cut(s) 242, 840, 1065
SfaNI GCATC 5 cut(s) 115, 456, 463, 1018, 1618
SfcI CTRYAG 3 cut(s) 777, 1078, 1361
Sfr274I CTCGAG 1 cut(s) 533
SfuI TTCGAA 1 cut(s) 1560
SlaI CTCGAG 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 533, 839
SmoI CTYRAG 2 cut(s) 533, 839
SphI GCATGC 1 cut(s) 1635
SseBI AGGCCT 1 cut(s) 1175
SsiI CCGC 1 cut(s) 56
SspI AATATT 2 cut(s) 425, 662
SspMI CTAG 1 cut(s) 849
SstI GAGCTC 1 cut(s) 242
StuI AGGCCT 1 cut(s) 1175
StyD4I CCNGG 2 cut(s) 788, 1566
StyI CCWWGG 2 cut(s) 24, 1551
TaaI ACNGT 4 cut(s) 674, 924, 1054, 1372
TaiI ACGT 2 cut(s) 189, 1295
TaqI TCGA 4 cut(s) 233, 534, 1560, 1620
TatI WGTACW 3 cut(s) 857, 919, 924
TfiI GAWTC 9 cut(s) 221, 443, 785, 891, 1008, 1041, 1184, 1336, 1545
Tru1I TTAA 3 cut(s) 432, 750, 1191
Tru9I TTAA 3 cut(s) 432, 750, 1191
TscAI CASTG 3 cut(s) 679, 992, 1087
TseFI GTSAC 2 cut(s) 1082, 1288
TseI GCWGC 2 cut(s) 1214, 1217
Tsp45I GTSAC 2 cut(s) 1082, 1288
TspGWI ACGGA 3 cut(s) 1083, 1607, 1638
TspRI CASTG 3 cut(s) 679, 992, 1087
XapI RAATTY 3 cut(s) 358, 933, 1125
XceI RCATGY 2 cut(s) 1635, 1679
XhoI CTCGAG 1 cut(s) 533
XspI CTAG 1 cut(s) 849
ZraI GACGTC 1 cut(s) 1293
ZrmI AGTACT 1 cut(s) 926
Zsp2I ATGCAT 2 cut(s) 130, 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.