MD11G1027600.v1.1

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0023543
Physical Location & Seq
Reverse (-)
2407205 .. 2407615
411 bp
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UTR
Exon/CDS
Intron
MD11G1027600.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCTCGTACCAAGCAGACTGCTCGCAAGTCCACCGGAGGAAAGGCACCAAGGAAGCAGCTGGCAACTAAGGCTGCTAGGAAGTCTGCTCCGGCGACCGGTGGAGTGAAGAAGCCCCACCGCTTCAGGCCGGGAACTGTGGCTCTGAGGGAAATCAGGAAGTACCAGAAGAGCACTGAGCTCCTGATCCGCAAGCTTCCGTTCCAGAGGCTTGTGAGGGAGATTGCACAGGACTTCAAGACCGATCTGAGGTTCCAGAGCAGCGCGGTGTCGGCGCTCCAGGAGGCGGCGGAGGCGTACTTGGTGGGTCTGTTTGAGGACACCAACCTCTGCGCCATTCATGCCAAGAGGGTCACCATAATGCCCAAGGACATCCAGCTTGCTCGCAGGATTAGGGGCGAGAGGGCTTAA

Protein Analysis

137

Amino Acids

15.28

Weight (kDa)

11.29

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 6.7e-39 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023543)

Species Orthologous Gene IDs
malus_domestica MD11G1027400.v1.1 MD11G1027600.v1.1
pyrus_communis pycom03g01940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AccII CGCG 1 cut(s) 266
AciI CCGC 5 cut(s) 121, 190, 266, 287, 290
AclWI GGATC 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 109
AfaI GTAC 3 cut(s) 10, 164, 299
AfiI CCNNNNNNNGG 3 cut(s) 98, 249, 286
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 4 cut(s) 61, 181, 196, 379
AluI AGCT 4 cut(s) 61, 181, 196, 379
Alw21I GWGCWC 2 cut(s) 176, 183
AlwI GGATC 1 cut(s) 181
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 58, 74, 261
AsiGI ACCGGT 1 cut(s) 98
AspLEI GCGC 3 cut(s) 266, 277, 335
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 1 cut(s) 346
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 176, 183
BbvI GCAGC 3 cut(s) 61, 70, 273
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 281
BcnI CCSGG 1 cut(s) 132
BfaI CTAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 278
BisI GCNGC 4 cut(s) 59, 75, 262, 288
BlsI GCNGC 4 cut(s) 60, 76, 263, 289
Bme1390I CCNGG 2 cut(s) 132, 281
BmiI GGNNCC 2 cut(s) 48, 254
BmrFI CCNGG 2 cut(s) 132, 281
BpmI CTGGAG 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 50, 366
BsaWI WCCGGW 2 cut(s) 35, 98
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 249, 286
Bse118I RCCGGY 1 cut(s) 98
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 50, 366
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 3 cut(s) 98, 249, 286
BseMII CTCAG 3 cut(s) 137, 168, 239
BseXI GCAGC 3 cut(s) 61, 70, 273
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 130
BshNI GGYRCC 1 cut(s) 46
BshTI ACCGGT 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 176, 183
BsiSI CCGG 4 cut(s) 36, 92, 99, 131
BslI CCNNNNNNNGG 3 cut(s) 98, 249, 286
BsnI GGCC 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 176, 183
Bsp143I GATC 2 cut(s) 186, 244
BspACI CCGC 5 cut(s) 121, 190, 266, 287, 290
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 3 cut(s) 138, 169, 240
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 2 cut(s) 48, 254
BspPI GGATC 1 cut(s) 181
BspQI GCTCTTC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 50, 366
BssMI GATC 2 cut(s) 186, 244
BssT1I CCWWGG 2 cut(s) 50, 366
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 5 cut(s) 25, 63, 194, 381, 385
BstDEI CTNAG 4 cut(s) 69, 146, 177, 248
BstEII GGTNACC 1 cut(s) 352
BstF5I GGATG 1 cut(s) 372
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 3 cut(s) 266, 277, 335
BstKTI GATC 2 cut(s) 189, 247
BstMBI GATC 2 cut(s) 186, 244
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
BstNI CCWGG 1 cut(s) 281
BstPI GGTNACC 1 cut(s) 352
BstSCI CCNGG 2 cut(s) 130, 279
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 3 cut(s) 61, 70, 273
BsuRI GGCC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 5 cut(s) 25, 63, 194, 381, 385
CfoI GCGC 3 cut(s) 266, 277, 335
Cfr10I RCCGGY 1 cut(s) 98
Csp6I GTAC 3 cut(s) 9, 163, 298
CspAI ACCGGT 1 cut(s) 98
CviAII CATG 1 cut(s) 341
CviQI GTAC 3 cut(s) 9, 163, 298
DdeI CTNAG 4 cut(s) 69, 146, 177, 248
DpnI GATC 2 cut(s) 188, 246
DpnII GATC 2 cut(s) 186, 244
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 1 cut(s) 305
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 2 cut(s) 50, 366
Eco24I GRGCYC 1 cut(s) 183
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 109
Eco91I GGTNACC 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 181
EcoO65I GGTNACC 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 2 cut(s) 50, 366
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 2 cut(s) 50, 366
FaeI CATG 1 cut(s) 344
FaiI YATR 2 cut(s) 342, 359
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 4 cut(s) 59, 75, 262, 288
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 4 cut(s) 59, 75, 262, 288
FspBI CTAG 1 cut(s) 78
GlaI GCGC 3 cut(s) 265, 276, 334
GluI GCNGC 4 cut(s) 59, 75, 262, 288
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 1 cut(s) 130
HapII CCGG 4 cut(s) 36, 92, 99, 131
HhaI GCGC 3 cut(s) 266, 277, 335
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 3 cut(s) 264, 275, 333
HinP1I GCGC 3 cut(s) 264, 275, 333
HindIII AAGCTT 1 cut(s) 194
HpaII CCGG 4 cut(s) 36, 92, 99, 131
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 147, 249
Hpy188III TCNNGA 5 cut(s) 157, 184, 205, 238, 256
Hpy8I GTNNAC 1 cut(s) 33
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 227
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
HpyF3I CTNAG 4 cut(s) 69, 146, 177, 248
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 3 cut(s) 264, 275, 333
Kzo9I GATC 2 cut(s) 186, 244
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 3 cut(s) 94, 186, 282
Lsp1109I GCAGC 3 cut(s) 61, 70, 273
MaeI CTAG 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 188, 246
MboI GATC 2 cut(s) 186, 244
MboII GAAGA 2 cut(s) 121, 181
MhlI GDGCHC 2 cut(s) 176, 183
MseI TTAA 1 cut(s) 409
MslI CAYNNNNRTG 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 4 cut(s) 36, 92, 99, 131
MspR9I CCNGG 2 cut(s) 132, 281
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
NciI CCSGG 1 cut(s) 132
NdeII GATC 2 cut(s) 186, 244
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 2 cut(s) 48, 254
NmuCI GTSAC 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 279
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 4 cut(s) 60, 76, 263, 289
Psp124BI GAGCTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 279
PspEI GGTNACC 1 cut(s) 352
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 2 cut(s) 48, 254
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 3 cut(s) 10, 164, 299
RsaNI GTAC 3 cut(s) 9, 163, 298
RseI CAYNNNNRTG 1 cut(s) 359
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 4 cut(s) 59, 75, 262, 288
Sau3AI GATC 2 cut(s) 186, 244
ScrFI CCNGG 2 cut(s) 132, 281
SduI GDGCHC 2 cut(s) 176, 183
SetI ASST 6 cut(s) 63, 183, 198, 254, 330, 381
SmiMI CAYNNNNRTG 1 cut(s) 359
SsiI CCGC 5 cut(s) 121, 190, 266, 287, 290
SspMI CTAG 1 cut(s) 78
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 130, 279
StyI CCWWGG 2 cut(s) 50, 366
TaaI ACNGT 1 cut(s) 139
TaqII GACCGA 1 cut(s) 257
TauI GCSGC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 3 cut(s) 58, 74, 261
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 1 cut(s) 181
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.