MD11G1213900.v1.1

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0028925
Physical Location & Seq
Forward (+)
31255625 .. 31266275
10651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1213900.v1.1.491

Sequence Viewer

Length: 783 bp
ATGGAGGCGCCACCATTCGCCTCTTCCACCAAGACGCTACCGTTCGCTCAGCCTCCCCTTCCTCCCTTCTATTCCTTCCCAATACTCGCCTCAATTTCAGACGTCCCACAAACTTCTCCGTCCGTGCCTCCGCCGCCGCAGATTCCGGTGCCCCCTCTCCTTCTCCCTCCCCTCTCTCTAATATCGGTGACTCTGCTCGACTACGGCGCCGGCAATGTTCGCGGCGTGAGGAATGCCATTCGCCACCTTGGCTTCGACGTCAAAGATGTCCAAACTCCGGAAGACATTCTGAATGCGGAACGCCTAGTTTTTCCTGGTGTGGGTGCTTTCGCTGCTGCCATGGATGTGCTGAATAAGAATGGGATGGCGGAAGCAATCTGTTCGTATATGGAGAAGGATCGACCCTTTCTAGGCATTTGTCTTGGACTTCAACTCCTTTTTGAATCCAGTGAAGAGAATGGACCAGTGAAGGGTCTTGGCTTGATACCCGGAGTGGTTGGGCGTTTTGATTCATCAAATGGTTTTAGAGTTCCACACATTGGATGGAATGCTTTACAGATTGAAAAAGACTCATTAATTTTGGATGATGTTGGAAGCCATCATGTCTGTTTCGTTCATTCTTACAGAGCCATGCAATCAGATGAAAACAAAGACTGGGTTTCATCTACTTGCAACTATGGAGACAATTTTATTGCGTCTATTAGAAGGGGAAATGTGCATGCAGTTCAATTCCACCCAGAAAAGAGTGGAGGCAAGATATCATTCCTCTCTCCCGTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

261

Amino Acids

28.12

Weight (kDa)

5.6

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 65 - 251 4.5e-23 Glutamine amidotransferase class-I
SNO PF01174 71 - 250 4.3e-07 SNO glutamine amidotransferase family
GATase_3 PF07685 87 - 164 8.9e-08 CobB/CobQ-like glutamine amidotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028925)

Species Orthologous Gene IDs
malus_domestica MD11G1213900.v1.1
pyrus_communis pycom11g18850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 105, 261
AccB1I GGYRCC 3 cut(s) 7, 148, 206
AccB7I CCANNNNNTGG 1 cut(s) 539
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 6 cut(s) 131, 134, 137, 222, 296, 368
AclWI GGATC 1 cut(s) 405
AcyI GRCGYC 4 cut(s) 8, 102, 207, 258
AfiI CCNNNNNNNGG 5 cut(s) 277, 320, 410, 470, 539
AgsI TTSAA 4 cut(s) 431, 443, 563, 728
AjnI CCWGG 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 1 cut(s) 405
Aor13HI TCCGGA 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 332, 335
AseI ATTAAT 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 285
AspLEI GCGC 2 cut(s) 10, 209
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 489
AsuHPI GGTGA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 461
BaeGI GKGCMC 1 cut(s) 153
BanI GGYRCC 3 cut(s) 7, 148, 206
BbsI GAAGAC 1 cut(s) 288
BbvI GCAGC 2 cut(s) 319, 322
BccI CCATC 3 cut(s) 358, 537, 606
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 363, 397
BciT130I CCWGG 1 cut(s) 315
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 1 cut(s) 675
BfaI CTAG 2 cut(s) 305, 410
BfoI RGCGCY 2 cut(s) 11, 210
BglI GCCNNNNNGGC 1 cut(s) 249
BisI GCNGC 5 cut(s) 134, 137, 223, 333, 336
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 5 cut(s) 135, 138, 224, 334, 337
Bme1390I CCNGG 2 cut(s) 315, 489
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 3 cut(s) 9, 150, 208
BmrFI CCNGG 2 cut(s) 315, 489
BmrI ACTGGG 1 cut(s) 664
BmuI ACTGGG 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 48
BpuMI CCSGG 1 cut(s) 489
BsaHI GRCGYC 4 cut(s) 8, 102, 207, 258
BsaJI CCNNGG 2 cut(s) 247, 339
BsaWI WCCGGW 2 cut(s) 145, 277
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 5 cut(s) 277, 320, 410, 470, 539
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 3 cut(s) 447, 464, 659
Bse3DI GCAATG 1 cut(s) 220
BseAI TCCGGA 1 cut(s) 277
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 247, 339
BseGI GGATG 4 cut(s) 349, 369, 548, 589
BseLI CCNNNNNNNGG 5 cut(s) 277, 320, 410, 470, 539
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 3 cut(s) 447, 464, 659
BseSI GKGCMC 1 cut(s) 153
BseXI GCAGC 2 cut(s) 319, 322
Bsh1236I CGCG 1 cut(s) 222
BshNI GGYRCC 3 cut(s) 7, 148, 206
BsiSI CCGG 4 cut(s) 146, 210, 278, 489
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 5 cut(s) 277, 320, 410, 470, 539
BsmAI GTCTC 1 cut(s) 675
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 3 cut(s) 238, 298, 553
Bsp1286I GDGCHC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 1 cut(s) 397
Bsp1720I GCTNAGC 1 cut(s) 48
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 6 cut(s) 131, 134, 137, 222, 296, 368
BspCNI CTCAG 1 cut(s) 61
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 3 cut(s) 9, 150, 208
BspPI GGATC 1 cut(s) 405
BspT107I GGYRCC 3 cut(s) 7, 148, 206
BsrDI GCAATG 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 3 cut(s) 447, 464, 659
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 247, 339
BssMI GATC 1 cut(s) 397
BssNI GRCGYC 4 cut(s) 8, 102, 207, 258
BssT1I CCWWGG 2 cut(s) 247, 339
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 2 cut(s) 28, 447
BstACI GRCGYC 4 cut(s) 8, 102, 207, 258
BstC8I GCNNGC 2 cut(s) 211, 720
BstDEI CTNAG 1 cut(s) 48
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 4 cut(s) 349, 369, 548, 589
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 2 cut(s) 11, 210
BstHHI GCGC 2 cut(s) 10, 209
BstKTI GATC 1 cut(s) 400
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 1 cut(s) 397
BstMWI GCNNNNNNNGC 4 cut(s) 133, 219, 249, 332
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 722
BstSCI CCNGG 2 cut(s) 313, 487
BstSLI GKGCMC 1 cut(s) 153
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 2 cut(s) 319, 322
BstV2I GAAGAC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 4 cut(s) 349, 369, 548, 589
BtsIMutI CAGTG 2 cut(s) 454, 471
Cac8I GCNNGC 2 cut(s) 211, 720
CfoI GCGC 2 cut(s) 10, 209
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 461
CseI GACGC 2 cut(s) 43, 684
CviAII CATG 4 cut(s) 340, 602, 631, 719
CviJI RGCY 5 cut(s) 52, 252, 480, 597, 629
CviKI_1 RGCY 5 cut(s) 52, 252, 480, 597, 629
DdeI CTNAG 1 cut(s) 48
DinI GGCGCC 2 cut(s) 9, 208
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 28, 447
EarI CTCTTC 2 cut(s) 28, 447
EciI GGCGGA 2 cut(s) 120, 383
Eco130I CCWWGG 2 cut(s) 247, 339
Eco32I GATATC 1 cut(s) 759
Eco47I GGWCC 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 759
EcoT14I CCWWGG 2 cut(s) 247, 339
EgeI GGCGCC 2 cut(s) 9, 208
EheI GGCGCC 2 cut(s) 9, 208
ErhI CCWWGG 2 cut(s) 247, 339
FaeI CATG 4 cut(s) 343, 605, 634, 722
FaiI YATR 7 cut(s) 341, 387, 389, 603, 632, 678, 720
FaqI GGGAC 1 cut(s) 89
FatI CATG 4 cut(s) 339, 601, 630, 718
Fnu4HI GCNGC 5 cut(s) 134, 137, 223, 333, 336
FokI GGATG 4 cut(s) 356, 376, 555, 596
Fsp4HI GCNGC 5 cut(s) 134, 137, 223, 333, 336
FspBI CTAG 2 cut(s) 305, 410
GlaI GCGC 2 cut(s) 9, 208
GluI GCNGC 5 cut(s) 134, 137, 223, 333, 336
HaeII RGCGCY 2 cut(s) 11, 210
HapII CCGG 4 cut(s) 146, 210, 278, 489
HgaI GACGC 2 cut(s) 43, 684
HhaI GCGC 2 cut(s) 10, 209
Hin1I GRCGYC 4 cut(s) 8, 102, 207, 258
Hin1II CATG 4 cut(s) 343, 605, 634, 722
Hin6I GCGC 2 cut(s) 8, 207
HinP1I GCGC 2 cut(s) 8, 207
HinfI GANTC 5 cut(s) 142, 190, 443, 509, 569
HpaII CCGG 4 cut(s) 146, 210, 278, 489
HphI GGTGA 1 cut(s) 199
Hpy188I TCNGA 3 cut(s) 100, 291, 640
Hpy188III TCNNGA 1 cut(s) 278
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 7 cut(s) 68, 76, 85, 170, 388, 463, 699
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 2 cut(s) 102, 258
HpyCH4V TGCA 4 cut(s) 634, 672, 718, 722
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 219, 249, 332
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 2 cut(s) 102, 258
Hsp92I GRCGYC 4 cut(s) 8, 102, 207, 258
Hsp92II CATG 4 cut(s) 343, 605, 634, 722
HspAI GCGC 2 cut(s) 8, 207
KasI GGCGCC 2 cut(s) 7, 206
Kpn2I TCCGGA 1 cut(s) 277
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 1 cut(s) 397
Lsp1109I GCAGC 2 cut(s) 319, 322
MaeI CTAG 2 cut(s) 305, 410
MaeII ACGT 2 cut(s) 102, 258
MaeIII GTNAC 1 cut(s) 187
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 3 cut(s) 15, 293, 464
MhlI GDGCHC 1 cut(s) 153
MluCI AATT 4 cut(s) 93, 576, 685, 728
Mly113I GGCGCC 2 cut(s) 8, 207
MlyI GAGTC 2 cut(s) 184, 563
MmeI TCCRAC 1 cut(s) 571
MroI TCCGGA 1 cut(s) 277
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 1 cut(s) 575
MslI CAYNNNNRTG 1 cut(s) 344
MspI CCGG 4 cut(s) 146, 210, 278, 489
MspR9I CCNGG 2 cut(s) 315, 489
Mva1269I GAATGC 3 cut(s) 238, 298, 553
MvaI CCWGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 4 cut(s) 133, 219, 249, 332
NaeI GCCGGC 1 cut(s) 211
NarI GGCGCC 2 cut(s) 8, 207
NciI CCSGG 1 cut(s) 489
NcoI CCATGG 1 cut(s) 339
NdeII GATC 1 cut(s) 397
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 4 cut(s) 343, 605, 634, 722
NlaIV GGNNCC 3 cut(s) 9, 150, 208
NmuCI GTSAC 1 cut(s) 187
NspI RCATGY 1 cut(s) 722
PaeI GCATGC 1 cut(s) 722
PctI GAATGC 3 cut(s) 238, 298, 553
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 3 cut(s) 142, 443, 509
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 5 cut(s) 135, 138, 224, 334, 337
PleI GAGTC 2 cut(s) 184, 563
PluTI GGCGCC 2 cut(s) 11, 210
PpsI GAGTC 2 cut(s) 184, 563
PshBI ATTAAT 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 9, 150, 208
PspPI GGNCC 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 5 cut(s) 134, 137, 223, 333, 336
Sau3AI GATC 1 cut(s) 397
Sau96I GGNCC 1 cut(s) 461
SchI GAGTC 2 cut(s) 184, 563
ScrFI CCNGG 2 cut(s) 315, 489
SduI GDGCHC 1 cut(s) 153
SetI ASST 3 cut(s) 105, 249, 261
SfoI GGCGCC 2 cut(s) 9, 208
SinI GGWCC 1 cut(s) 461
SmiMI CAYNNNNRTG 1 cut(s) 344
SphI GCATGC 1 cut(s) 722
Sse9I AATT 4 cut(s) 93, 576, 685, 728
SsiI CCGC 6 cut(s) 131, 134, 137, 222, 296, 368
SspDI GGCGCC 2 cut(s) 7, 206
SspMI CTAG 2 cut(s) 305, 410
StyD4I CCNGG 2 cut(s) 313, 487
StyI CCWWGG 2 cut(s) 247, 339
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 2 cut(s) 105, 261
TaqI TCGA 3 cut(s) 198, 255, 400
TasI AATT 4 cut(s) 93, 576, 685, 728
TauI GCSGC 3 cut(s) 136, 139, 225
TfiI GAWTC 3 cut(s) 142, 443, 509
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 2 cut(s) 454, 471
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 2 cut(s) 332, 335
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 4 cut(s) 501, 605, 651, 657
TspGWI ACGGA 2 cut(s) 108, 112
TspRI CASTG 2 cut(s) 454, 471
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 461
VspI ATTAAT 1 cut(s) 575
XceI RCATGY 1 cut(s) 722
XcmI CCANNNNNNNNNTGG 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 2 cut(s) 305, 410
ZraI GACGTC 2 cut(s) 103, 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.