MD11G1286700.v1.1

DNA repair helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0028918
Physical Location & Seq
Forward (+)
40636517 .. 40637839
1323 bp
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UTR
Exon/CDS
Intron
MD11G1286700.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGAGAAGACGCTCGCCGAGCTCAAGCTCCTCCACAATTACCAGGTCAAGCATCTGGGTCCGCAGGCCCAGATCTTGGCGGTCGGTCTTTCATCGAGGAAGAATCTTTGTGTTAATTCGACAGTGTTGGCGGCGGAGAATCGGGATTCTGTGGATGCGGCTTGCTGGAAATTGACTGCCAGCTGGGTCAGGAACTTGGCGGCGGAAAACCCAAATGTGCCAACTTGTGAGTTTTATGAGGAGTATGAGAAAGCTGGGTCTGGAGCTGTTTTGCCTCCTGGGGTTTATACATTGCAAGATTTGAGGGCGTATGGGAAGCAGAGATGGTGGTTTATAGTTATCAGTACATGCTTGATCCCAAAGTGGCTGGGATTATTTCCAAGGAAATGCAAAAGGAGTCTGTTGTTGTGTTTGATGCGGCTCATATTATCGATAATGTGTGTATTGAAGCGTTTAGTGCTAGTGCGAGGAGGCAGACAATTGAGGGTGCCAGGAGGAATTAGTAAGATGCAGCATGAAATTGAAAGATTGCTTAAAGAATACAACCGGCTAGTTGAGGGTTTGGCACAAGGAGGAAACCTACCTAGAGGCTGTGCCTGGAAATATTCGTAA

Protein Analysis

204

Amino Acids

22.94

Weight (kDa)

9.64

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD_2 PF06733 1 - 107 1e-14 DEAD_2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028918)

Species Orthologous Gene IDs
malus_domestica MD11G1286700.v1.1
pyrus_communis pycom11g25420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 487
AccB7I CCANNNNNTGG 1 cut(s) 76
AciI CCGC 8 cut(s) 62, 80, 131, 134, 158, 200, 203, 418
AclWI GGATC 1 cut(s) 349
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 2 cut(s) 448, 524
AjnI CCWGG 4 cut(s) 42, 277, 490, 596
AluBI AGCT 5 cut(s) 22, 28, 183, 254, 266
AluI AGCT 5 cut(s) 22, 28, 183, 254, 266
Alw21I GWGCWC 1 cut(s) 24
AlwI GGATC 1 cut(s) 349
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 511
AspS9I GGNCC 2 cut(s) 59, 67
AvaII GGWCC 1 cut(s) 59
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 523
BccI CCATC 1 cut(s) 318
BciT130I CCWGG 4 cut(s) 44, 279, 492, 598
BfaI CTAG 3 cut(s) 461, 551, 585
BglII AGATCT 1 cut(s) 72
BisI GCNGC 5 cut(s) 132, 159, 201, 419, 512
BlsI GCNGC 5 cut(s) 133, 160, 202, 420, 513
Bme1390I CCNGG 4 cut(s) 44, 279, 492, 598
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 59, 67
BmiI GGNNCC 2 cut(s) 60, 489
BmrFI CCNGG 4 cut(s) 44, 279, 492, 598
BmsI GCATC 4 cut(s) 61, 145, 405, 498
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 8
Bsa29I ATCGAT 1 cut(s) 431
BsaJI CCNNGG 2 cut(s) 278, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 290
BseBI CCWGG 4 cut(s) 44, 279, 492, 598
BseCI ATCGAT 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 278, 380
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 290
BseRI GAGGAG 3 cut(s) 20, 254, 483
BseXI GCAGC 1 cut(s) 523
BseYI CCCAGC 3 cut(s) 183, 254, 367
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 487
BshVI ATCGAT 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 1 cut(s) 547
BslI CCNNNNNNNGG 1 cut(s) 76
BsnI GGCC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 2 cut(s) 72, 354
BspACI CCGC 8 cut(s) 62, 80, 131, 134, 158, 200, 203, 418
BspANI GGCC 1 cut(s) 68
BspDI ATCGAT 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 60, 489
BspPI GGATC 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 487
BsrDI GCAATG 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 546
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 2 cut(s) 278, 380
BssMI GATC 2 cut(s) 72, 354
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 4 cut(s) 44, 279, 492, 598
Bst4CI ACNGT 1 cut(s) 124
BstC8I GCNNGC 4 cut(s) 15, 66, 163, 181
BstF5I GGATG 1 cut(s) 160
BstKTI GATC 2 cut(s) 75, 357
BstMBI GATC 2 cut(s) 72, 354
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 2 cut(s) 19, 457
BstNI CCWGG 4 cut(s) 44, 279, 492, 598
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 4 cut(s) 42, 277, 490, 596
BstV1I GCAGC 1 cut(s) 523
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
Bsu15I ATCGAT 1 cut(s) 431
BsuRI GGCC 1 cut(s) 68
BsuTUI ATCGAT 1 cut(s) 431
BtsCI GGATG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 4 cut(s) 15, 66, 163, 181
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 2 cut(s) 59, 67
ClaI ATCGAT 1 cut(s) 431
CseI GACGC 1 cut(s) 19
CsiI ACCWGGT 1 cut(s) 42
Csp6I GTAC 1 cut(s) 345
CviAII CATG 2 cut(s) 348, 515
CviQI GTAC 1 cut(s) 345
DpnI GATC 2 cut(s) 74, 356
DpnII GATC 2 cut(s) 72, 354
EciI GGCGGA 2 cut(s) 149, 218
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 380
Eco24I GRGCYC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 22
EcoRII CCWGG 4 cut(s) 42, 277, 490, 596
EcoT14I CCWWGG 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 2 cut(s) 351, 518
FaiI YATR 8 cut(s) 237, 246, 288, 312, 335, 349, 425, 516
FatI CATG 2 cut(s) 347, 514
Fnu4HI GCNGC 5 cut(s) 132, 159, 201, 419, 512
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 5 cut(s) 132, 159, 201, 419, 512
FspBI CTAG 3 cut(s) 461, 551, 585
GluI GCNGC 5 cut(s) 132, 159, 201, 419, 512
GsaI CCCAGC 3 cut(s) 187, 258, 371
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 547
HgaI GACGC 1 cut(s) 19
Hin1II CATG 2 cut(s) 351, 518
HinfI GANTC 4 cut(s) 103, 139, 146, 397
HpaII CCGG 1 cut(s) 547
Hpy188III TCNNGA 3 cut(s) 143, 190, 261
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4V TGCA 3 cut(s) 295, 390, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 457
Hsp92II CATG 2 cut(s) 351, 518
Kzo9I GATC 2 cut(s) 72, 354
LmnI GCTCC 2 cut(s) 33, 263
Lsp1109I GCAGC 1 cut(s) 523
LweI GCATC 4 cut(s) 61, 145, 405, 498
MabI ACCWGGT 1 cut(s) 42
MaeI CTAG 3 cut(s) 461, 551, 585
MalI GATC 2 cut(s) 74, 356
MboI GATC 2 cut(s) 72, 354
MboII GAAGA 2 cut(s) 19, 112
MfeI CAATTG 1 cut(s) 479
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 6 cut(s) 37, 115, 170, 479, 498, 519
MlyI GAGTC 1 cut(s) 406
MseI TTAA 2 cut(s) 114, 534
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 1 cut(s) 547
MspR9I CCNGG 4 cut(s) 44, 279, 492, 598
MunI CAATTG 1 cut(s) 479
MvaI CCWGG 4 cut(s) 44, 279, 492, 598
MwoI GCNNNNNNNGC 2 cut(s) 19, 457
NdeII GATC 2 cut(s) 72, 354
NlaIII CATG 2 cut(s) 351, 518
NlaIV GGNNCC 2 cut(s) 60, 489
NmeAIII GCCGAG 1 cut(s) 43
NspI RCATGY 1 cut(s) 351
PfeI GAWTC 3 cut(s) 103, 139, 146
PflMI CCANNNNNTGG 1 cut(s) 76
PkrI GCNGC 5 cut(s) 133, 160, 202, 420, 513
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
Psp124BI GAGCTC 1 cut(s) 24
Psp6I CCWGG 4 cut(s) 42, 277, 490, 596
PspFI CCCAGC 3 cut(s) 183, 254, 367
PspGI CCWGG 4 cut(s) 42, 277, 490, 596
PspN4I GGNNCC 2 cut(s) 60, 489
PspPI GGNCC 2 cut(s) 59, 67
PsuI RGATCY 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 183
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 2 cut(s) 114, 534
SatI GCNGC 5 cut(s) 132, 159, 201, 419, 512
Sau3AI GATC 2 cut(s) 72, 354
Sau96I GGNCC 2 cut(s) 59, 67
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 4 cut(s) 44, 279, 492, 598
SduI GDGCHC 1 cut(s) 24
SetI ASST 8 cut(s) 24, 30, 48, 185, 256, 268, 582, 586
SexAI ACCWGGT 1 cut(s) 42
SfaNI GCATC 4 cut(s) 61, 145, 405, 498
SinI GGWCC 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 6 cut(s) 37, 115, 170, 479, 498, 519
SsiI CCGC 8 cut(s) 62, 80, 131, 134, 158, 200, 203, 418
SspI AATATT 1 cut(s) 605
SspMI CTAG 3 cut(s) 461, 551, 585
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 4 cut(s) 42, 277, 490, 596
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 1 cut(s) 124
TaqI TCGA 3 cut(s) 95, 119, 431
TaqII GACCGA 1 cut(s) 74
TasI AATT 6 cut(s) 37, 115, 170, 479, 498, 519
TatI WGTACW 1 cut(s) 344
TauI GCSGC 4 cut(s) 134, 161, 203, 421
TfiI GAWTC 3 cut(s) 103, 139, 146
Tru1I TTAA 2 cut(s) 114, 534
Tru9I TTAA 2 cut(s) 114, 534
TscAI CASTG 1 cut(s) 129
TseI GCWGC 1 cut(s) 511
TspDTI ATGAA 2 cut(s) 81, 531
TspRI CASTG 1 cut(s) 129
Van91I CCANNNNNTGG 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 59
XceI RCATGY 1 cut(s) 351
XspI CTAG 3 cut(s) 461, 551, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.