MD12G1025000.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0028710
Physical Location & Seq
Reverse (-)
2685155 .. 2685864
710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1025000.v1.1.491

Sequence Viewer

Length: 465 bp
ATGTTATTTACAGATTTGGCCGAAATGGTACACTCAATCATGATGAGAGGAAAGATTGTGGTTTGTATCATGGATAAAATCGACGACGATGTAATGGAGAAAGCAAAGGTCGTAAAAGAAGGCGGAGCAGTTGGAATGATTCTCATTGATCAATTTGATAACTTCAACGAGCAGCTTCCTTCTTTTGATCTCCCTACTTCGGCCCGATTGCAAGTATCTTGGAGACTCGGGGAAGTAATTGGTGTAGAACTGGCACCAAAGATGGCGTTGTTTTCGTCGAGAGGACCTAATTCTGTAACACCTGACATCATCAAACCTGACATCACTGCACCAGGAGTCAACATTTTAGCAGCAGCGCCACCGAGTAAAAATCAAGCCGTCGATGCACTTTCGTACAACATGCATTCAGGTACTTCAATGGTGTGTTCGCATGTCGCGGGTGTTGCAGCAGTTCTAAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

155

Amino Acids

16.47

Weight (kDa)

5.25

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 15 - 56 9.1e-06 PA domain
Peptidase_S8 PF00082 83 - 153 5.2e-11 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028710)

Species Orthologous Gene IDs
malus_domestica MD12G1025000.v1.1
pyrus_communis pycom12g02450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 253
AccII CGCG 1 cut(s) 437
AciI CCGC 2 cut(s) 123, 437
AcoI YGGCCR 1 cut(s) 18
AfaI GTAC 3 cut(s) 30, 395, 412
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 2 cut(s) 166, 417
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 2 cut(s) 175, 461
AluI AGCT 2 cut(s) 175, 461
Alw26I GTCTC 1 cut(s) 217
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 2 cut(s) 18, 201
ApeKI GCWGC 4 cut(s) 172, 350, 353, 446
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 202, 284
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 253
BbvI GCAGC 4 cut(s) 184, 362, 365, 458
BccI CCATC 1 cut(s) 256
BceAI ACGGC 1 cut(s) 362
BciT130I CCWGG 1 cut(s) 333
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 217
BfoI RGCGCY 1 cut(s) 359
BisI GCNGC 4 cut(s) 173, 351, 354, 447
BlsI GCNGC 4 cut(s) 174, 352, 355, 448
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 284
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 202, 284
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 373
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 199
BseNI ACTGG 1 cut(s) 255
BseXI GCAGC 4 cut(s) 184, 362, 365, 458
BsgI GTGCAG 1 cut(s) 312
Bsh1236I CGCG 1 cut(s) 437
BshFI GGCC 2 cut(s) 20, 203
BshNI GGYRCC 1 cut(s) 253
BsiHKCI CYCGRG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 217
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 2 cut(s) 20, 203
BsoBI CYCGRG 1 cut(s) 227
Bsp143I GATC 2 cut(s) 148, 187
BspACI CCGC 2 cut(s) 123, 437
BspANI GGCC 2 cut(s) 20, 203
BspFNI CGCG 1 cut(s) 437
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 255
BssMI GATC 2 cut(s) 148, 187
Bst2UI CCWGG 1 cut(s) 333
BstFNI CGCG 1 cut(s) 437
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 2 cut(s) 151, 190
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 2 cut(s) 148, 187
BstMWI GCNNNNNNNGC 2 cut(s) 383, 443
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 2 cut(s) 403, 434
BstSCI CCNGG 1 cut(s) 331
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 4 cut(s) 184, 362, 365, 458
BsuRI GGCC 2 cut(s) 20, 203
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 324
CciI TCATGA 1 cut(s) 39
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 202, 284
Csp6I GTAC 3 cut(s) 29, 394, 411
CviAII CATG 4 cut(s) 40, 70, 400, 431
CviJI RGCY 5 cut(s) 20, 175, 203, 377, 461
CviKI_1 RGCY 5 cut(s) 20, 175, 203, 377, 461
CviQI GTAC 3 cut(s) 29, 394, 411
DpnI GATC 2 cut(s) 150, 189
DpnII GATC 2 cut(s) 148, 187
EaeI YGGCCR 1 cut(s) 18
EciI GGCGGA 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 284
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 331
EcoT22I ATGCAT 1 cut(s) 405
FaeI CATG 4 cut(s) 43, 73, 403, 434
FaiI YATR 4 cut(s) 41, 71, 401, 432
FatI CATG 4 cut(s) 39, 69, 399, 430
FauI CCCGC 1 cut(s) 430
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 173, 351, 354, 447
Fsp4HI GCNGC 4 cut(s) 173, 351, 354, 447
GlaI GCGC 1 cut(s) 357
GluI GCNGC 4 cut(s) 173, 351, 354, 447
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 2 cut(s) 20, 203
HhaI GCGC 1 cut(s) 358
Hin1II CATG 4 cut(s) 43, 73, 403, 434
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 3 cut(s) 139, 225, 336
Hpy166II GTNNAC 2 cut(s) 31, 340
Hpy188III TCNNGA 2 cut(s) 40, 279
Hpy8I GTNNAC 2 cut(s) 31, 340
Hpy99I CGWCG 4 cut(s) 86, 89, 280, 383
HpyAV CCTTC 2 cut(s) 113, 189
HpyCH4V TGCA 5 cut(s) 211, 329, 386, 403, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 383, 443
Hsp92II CATG 4 cut(s) 43, 73, 403, 434
HspAI GCGC 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 2 cut(s) 148, 187
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 6 cut(s) 236, 315, 318, 330, 345, 393
Lsp1109I GCAGC 4 cut(s) 184, 362, 365, 458
LweI GCATC 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 295
MalI GATC 2 cut(s) 150, 189
MboI GATC 2 cut(s) 148, 187
MluCI AATT 3 cut(s) 152, 237, 289
MlyI GAGTC 2 cut(s) 219, 345
MmeI TCCRAC 1 cut(s) 112
MnlI CCTC 2 cut(s) 41, 275
Mph1103I ATGCAT 1 cut(s) 405
MseI TTAA 1 cut(s) 463
MspR9I CCNGG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 2 cut(s) 383, 443
NdeII GATC 2 cut(s) 148, 187
NlaIII CATG 4 cut(s) 43, 73, 403, 434
NlaIV GGNNCC 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 2 cut(s) 403, 434
PagI TCATGA 1 cut(s) 39
PctI GAATGC 1 cut(s) 403
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 4 cut(s) 174, 352, 355, 448
PleI GAGTC 2 cut(s) 219, 344
PpsI GAGTC 2 cut(s) 219, 344
PpuMI RGGWCCY 1 cut(s) 284
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 202, 284
PspPPI RGGWCCY 1 cut(s) 284
RsaI GTAC 3 cut(s) 30, 395, 412
RsaNI GTAC 3 cut(s) 29, 394, 411
SaqAI TTAA 1 cut(s) 463
SatI GCNGC 4 cut(s) 173, 351, 354, 447
Sau3AI GATC 2 cut(s) 148, 187
Sau96I GGNCC 2 cut(s) 202, 284
SchI GAGTC 2 cut(s) 219, 345
ScrFI CCNGG 1 cut(s) 333
SetI ASST 7 cut(s) 111, 177, 289, 304, 319, 412, 463
SfaNI GCATC 1 cut(s) 373
SinI GGWCC 1 cut(s) 284
Sse9I AATT 3 cut(s) 152, 237, 289
SsiI CCGC 2 cut(s) 123, 437
StyD4I CCNGG 1 cut(s) 331
TaqI TCGA 3 cut(s) 81, 278, 381
TasI AATT 3 cut(s) 152, 237, 289
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 1 cut(s) 463
Tru9I TTAA 1 cut(s) 463
TscAI CASTG 1 cut(s) 331
TseI GCWGC 4 cut(s) 172, 350, 353, 446
TspRI CASTG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 284
XceI RCATGY 2 cut(s) 403, 434
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.