MD12G1098100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024423
Physical Location & Seq
Forward (+)
15351642 .. 15352515
874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1098100.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGTGTTAGAGAGCAAGAATAATTGTCTACGGTTATTAAAATTGGGAGCACTTGAAAACCTCGTTGAAGCTCTTGAGGTGCTTCGGGTAATCACTTCAGAAGAACACAAACTGCAGGAAGTAATGAAAGCCGGAATCACCAAGGGAGAAGTGGCAAATGAACTAGTCCAGATTCTGAAGAAGTACAGGCATCCACCGATCAAAGTTTCAAGAATAAAGAGGTTTGCCATAGAGCTGGCAATCTGTATAATGAGAGACAAACCAAAAAATGTCCGAGTTTTCAGGGATTTGGGGATGGAAAATGAGCTGGAGTTTGTGCTAGAGACAACCGCAGAACTCAACATCTTCAACATTTTCTCTGGTACCGTAGGAATGAGCCATCACAGCACGACGATCCATTCACTGGTAAAGACTGCTTTGGGGTTGCAGGATGGAATGAAGCAGCATAATGCAGTATTCATCAAATTCATTACATACGTGGAAATCAAAATAATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.89

Weight (kDa)

9.1

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024423)

Species Orthologous Gene IDs
malus_domestica MD12G1098100.v1.1
rosa_multiflora Rmu_sc0000935.1_g000039 Rmu_sc0001556.1_g000004

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 403
AccI GTMKAC 1 cut(s) 28
AciI CCGC 1 cut(s) 330
AclWI GGATC 1 cut(s) 388
AcsI RAATTY 1 cut(s) 464
AcuI CTGAAG 2 cut(s) 81, 197
AfaI GTAC 2 cut(s) 185, 364
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 4 cut(s) 56, 68, 210, 349
AhlI ACTAGT 1 cut(s) 163
AluBI AGCT 3 cut(s) 71, 235, 307
AluI AGCT 3 cut(s) 71, 235, 307
Alw21I GWGCWC 1 cut(s) 52
Alw26I GTCTC 2 cut(s) 249, 317
AlwI GGATC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 175
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 1 cut(s) 464
Asp718I GGTACC 1 cut(s) 362
AsuHPI GGTGA 1 cut(s) 130
BanI GGYRCC 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 52
BbvI GCAGC 1 cut(s) 454
BccI CCATC 3 cut(s) 289, 387, 425
BcoDI GTCTC 2 cut(s) 249, 317
BcuI ACTAGT 1 cut(s) 163
BfaI CTAG 2 cut(s) 164, 320
BfmI CTRYAG 1 cut(s) 113
BisI GCNGC 1 cut(s) 443
BlsI GCNGC 1 cut(s) 444
BmiI GGNNCC 1 cut(s) 364
BmsI GCATC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 95
BsaAI YACGTR 1 cut(s) 478
BsaJI CCNNGG 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 3 cut(s) 190, 300, 436
BseLI CCNNNNNNNGG 1 cut(s) 403
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 454
BshNI GGYRCC 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 52
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 403
BsmAI GTCTC 2 cut(s) 249, 317
Bsp1286I GDGCHC 1 cut(s) 52
Bsp143I GATC 2 cut(s) 198, 393
BspACI CCGC 1 cut(s) 330
BspLI GGNNCC 1 cut(s) 364
BspMAI CTGCAG 1 cut(s) 117
BspPI GGATC 1 cut(s) 388
BspT107I GGYRCC 1 cut(s) 362
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 2 cut(s) 198, 393
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 33, 367
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 237
BstF5I GGATG 3 cut(s) 190, 300, 436
BstKTI GATC 2 cut(s) 201, 396
BstMAI GTCTC 2 cut(s) 249, 317
BstMBI GATC 2 cut(s) 198, 393
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 235
BtsCI GGATG 3 cut(s) 190, 300, 436
BtsIMutI CAGTG 1 cut(s) 401
Cac8I GCNNGC 1 cut(s) 237
CaiI CAGNNNCTG 1 cut(s) 175
Csp6I GTAC 2 cut(s) 184, 363
CviJI RGCY 5 cut(s) 71, 131, 235, 307, 378
CviKI_1 RGCY 5 cut(s) 71, 131, 235, 307, 378
CviQI GTAC 2 cut(s) 184, 363
DpnI GATC 2 cut(s) 200, 395
DpnII GATC 2 cut(s) 198, 393
Eco130I CCWWGG 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 81, 197
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaiI YATR 4 cut(s) 230, 248, 447, 475
FblI GTMKAC 1 cut(s) 28
Fnu4HI GCNGC 1 cut(s) 443
FokI GGATG 3 cut(s) 177, 307, 443
Fsp4HI GCNGC 1 cut(s) 443
FspBI CTAG 2 cut(s) 164, 320
GluI GCNGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 329
HapII CCGG 1 cut(s) 132
HinfI GANTC 2 cut(s) 135, 172
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 3 cut(s) 100, 177, 275
Hpy188III TCNNGA 3 cut(s) 74, 169, 210
Hpy8I GTNNAC 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 394
HpyCH4III ACNGT 2 cut(s) 33, 367
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 3 cut(s) 115, 427, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 477
KpnI GGTACC 1 cut(s) 366
Kzo9I GATC 2 cut(s) 198, 393
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 1 cut(s) 199
MaeI CTAG 2 cut(s) 164, 320
MaeII ACGT 1 cut(s) 477
MalI GATC 2 cut(s) 200, 395
MboI GATC 2 cut(s) 198, 393
MboII GAAGA 3 cut(s) 113, 190, 337
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 3 cut(s) 22, 41, 464
MnlI CCTC 3 cut(s) 70, 71, 213
MseI TTAA 1 cut(s) 38
MspI CCGG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 384
NdeII GATC 2 cut(s) 198, 393
NlaIV GGNNCC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 135, 172
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 1 cut(s) 444
Ppu21I YACGTR 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 364
PstI CTGCAG 1 cut(s) 117
PstNI CAGNNNCTG 1 cut(s) 175
RsaI GTAC 2 cut(s) 185, 364
RsaNI GTAC 2 cut(s) 184, 363
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 1 cut(s) 443
Sau3AI GATC 2 cut(s) 198, 393
SduI GDGCHC 1 cut(s) 52
SetI ASST 7 cut(s) 63, 73, 81, 224, 237, 309, 480
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SpeI ACTAGT 1 cut(s) 163
Sse9I AATT 3 cut(s) 22, 41, 464
SsiI CCGC 1 cut(s) 330
SspMI CTAG 2 cut(s) 164, 320
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 33, 367
TaiI ACGT 1 cut(s) 480
TasI AATT 3 cut(s) 22, 41, 464
TatI WGTACW 1 cut(s) 183
TfiI GAWTC 2 cut(s) 135, 172
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 1 cut(s) 408
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 5 cut(s) 140, 174, 448, 452, 457
TspRI CASTG 1 cut(s) 408
Van91I CCANNNNNTGG 1 cut(s) 403
XapI RAATTY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 28
XspI CTAG 2 cut(s) 164, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.