MD12G1194900.v1.1
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0028720
Physical Location & Seq
Forward (+)
27613772 .. 27614535
764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1194900.v1.1.491

Sequence Viewer

Length: 348 bp
ATGCACAGTGTTGGCCTTGTTCTCTTCCTGCTTATGAAGTTAAAGCACCTTAGGAGGATATTATTTTGTCATCTCATATATGGTTGTTTTTTCGCAGCTGGATCTATTCATATTAGCAATATCTTAACCGGAAAATGCCTGGCCAAAATAAATCCAAGCAATGGTGGTCCCAAGGTCGACAACTGTGAAGGCAACGCTGGCTCCTCAAGGAAGCAAAGCAACTCATCAGTGGTGAGCAGCACCGTCGTTGATGCTTTGAAAGACGTAACTGCTCTCTTCTATGATGAAGATCGCAACGAGATATATACAGGAAATAGGCATGGTTTTGTTCATGTGTGGTCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.65

Weight (kDa)

9.02

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 33 - 113 1.2e-21 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028720)

Species Orthologous Gene IDs
malus_domestica MD12G1194900.v1.1
pyrus_communis pycom12g18110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 161
AccI GTMKAC 1 cut(s) 177
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 141
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 1 cut(s) 259
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 1 cut(s) 98
AluI AGCT 1 cut(s) 98
AlwI GGATC 1 cut(s) 109
AoxI GGCC 2 cut(s) 13, 141
ApeKI GCWGC 2 cut(s) 95, 237
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 244
AvaII GGWCC 1 cut(s) 167
AxyI CCTNAGG 1 cut(s) 50
BalI TGGCCA 1 cut(s) 143
BbvI GCAGC 2 cut(s) 107, 249
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 140
BisI GCNGC 2 cut(s) 96, 238
BlsI GCNGC 2 cut(s) 97, 239
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 169, 202
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 190
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse21I CCTNAGG 1 cut(s) 50
Bse3DI GCAATG 1 cut(s) 166
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 171
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMI GCAATG 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 193
BseXI GCAGC 2 cut(s) 107, 249
BshFI GGCC 2 cut(s) 15, 143
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 153
BsnI GGCC 2 cut(s) 15, 143
Bsp143I GATC 2 cut(s) 101, 289
BspANI GGCC 2 cut(s) 15, 143
BspLI GGNNCC 2 cut(s) 169, 202
BspPI GGATC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 2 cut(s) 101, 289
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 8, 185, 244
Bst6I CTCTTC 2 cut(s) 29, 281
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 1 cut(s) 50
BstKTI GATC 2 cut(s) 104, 292
BstMBI GATC 2 cut(s) 101, 289
BstMWI GCNNNNNNNGC 1 cut(s) 198
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 107, 249
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
Bsu36I CCTNAGG 1 cut(s) 50
BsuRI GGCC 2 cut(s) 15, 143
BtsIMutI CAGTG 2 cut(s) 13, 234
Cac8I GCNNGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 167
CviAII CATG 2 cut(s) 320, 332
CviJI RGCY 4 cut(s) 15, 98, 143, 201
CviKI_1 RGCY 4 cut(s) 15, 98, 143, 201
DdeI CTNAG 1 cut(s) 50
DpnI GATC 2 cut(s) 103, 291
DpnII GATC 2 cut(s) 101, 289
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 2 cut(s) 29, 281
EarI CTCTTC 2 cut(s) 29, 281
Eco130I CCWWGG 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 2 cut(s) 323, 335
FaqI GGGAC 1 cut(s) 153
FatI CATG 2 cut(s) 319, 331
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 96, 238
Fsp4HI GCNGC 2 cut(s) 96, 238
GluI GCNGC 2 cut(s) 96, 238
HaeIII GGCC 2 cut(s) 15, 143
HapII CCGG 1 cut(s) 129
Hin1II CATG 2 cut(s) 323, 335
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HpaII CCGG 1 cut(s) 129
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 1 cut(s) 178
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 3 cut(s) 8, 185, 244
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 198
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 323, 335
Kzo9I GATC 2 cut(s) 101, 289
LmnI GCTCC 1 cut(s) 206
LpnPI CCDG 7 cut(s) 41, 84, 125, 142, 152, 183, 294
Lsp1109I GCAGC 2 cut(s) 107, 249
LweI GCATC 1 cut(s) 241
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 265
MalI GATC 2 cut(s) 103, 291
MboI GATC 2 cut(s) 101, 289
MboII GAAGA 3 cut(s) 16, 268, 299
MflI RGATCY 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 143
MluNI TGGCCA 1 cut(s) 143
MnlI CCTC 2 cut(s) 48, 214
Mox20I TGGCCA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 143
MseI TTAA 2 cut(s) 41, 125
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 198
NdeII GATC 2 cut(s) 101, 289
NlaIII CATG 2 cut(s) 323, 335
NlaIV GGNNCC 2 cut(s) 169, 202
PflMI CCANNNNNTGG 1 cut(s) 161
PkrI GCNGC 2 cut(s) 97, 239
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 169, 202
PspPI GGNCC 1 cut(s) 167
PsuI RGATCY 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 98
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 41, 125
SatI GCNGC 2 cut(s) 96, 238
Sau3AI GATC 2 cut(s) 101, 289
Sau96I GGNCC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 140
SetI ASST 4 cut(s) 51, 100, 177, 267
SfaNI GCATC 1 cut(s) 241
SinI GGWCC 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 3 cut(s) 8, 185, 244
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 177
Tru1I TTAA 2 cut(s) 41, 125
Tru9I TTAA 2 cut(s) 41, 125
TscAI CASTG 2 cut(s) 13, 234
TseI GCWGC 2 cut(s) 95, 237
TspDTI ATGAA 4 cut(s) 50, 98, 300, 320
TspRI CASTG 2 cut(s) 13, 234
Van91I CCANNNNNTGG 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.