Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0028255

Physical Location & Seq

Genomic Coordinates Forward (+)

3807877 .. 3809365

Gene Structure 1489 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1054300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGGGCAAGGATGTCGAAAACCCGAGTTATAAATCGCTGTACGTGAATGAAACCCGAGGAGTTTTCGGAATTGGTGCCGCGGTTCACTTTGCACCTTCATCATCTTCTTCTTCGTTCAAAAGGTTAGTTCTTGTCTTTCTTTTTGCCCTCGTTGATTTTGACGGGACACCCACACAAGTAGCTCATGTTGTAGCATCACTTGTGGAAGAAGGATTTACTGCACAGGTAGCTATACAGGCAGTGAGAAAGAAAGTCGGATACCAAATTCAAGTTCGCGCTGACGCCAATCGGAACTGGACATATGAAGAAGCTATCCAGTTTGGATCCTCGGTAAAGGATTGCGATCTGCAGTATTTAGAGGAACCTGTTCAAAATGAAGGCGATATTATCAGATTCTGTGAAGAAAGTGGGTTACTTGTGGCACTGGATGAAACTATTGACAGTATTAGAGAACATCCACTTGATAAGCTGGATGAAACTGTTATTAAACCTAGCGTTGTTGGCGGCTTCGAAAATACAGCAATCATTACCCGGTGGGCACAGCAGCACCAAAAAATGGCTGTTGTTAGTGCTGCGTTTGAAAGTGGCCTAGGTTTGTCAGCATATTCAGTTTTGCTGTTATCTCAACCAGAAGAATTCAGAAATCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0010109 GO:0019222 GO:0031323 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042548 GO:0042550 GO:0043467 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0050789 GO:0050794 GO:0051186 GO:0051188 GO:0065007 GO:0071704 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 MR_MLE_C TPP_enzyme_C TPP_enzyme_M_2 TPP_enzyme_N

Protein Analysis

217

Amino Acids

23.81

Weight (kDa)

4.8

Isoelectric Point (pI)

31.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_C	PF13378	77 - 202	2.5e-14	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028255)

Species	Orthologous Gene IDs
malus_domestica	MD13G1054300.v1.1
pyrus_communis	pycom13g04820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	30
AccB1I	GGYRCC	1 cut(s)	74
AccB7I	CCANNNNNTGG	1 cut(s)	556
AccII	CGCG	2 cut(s)	80, 276
AciI	CCGC	3 cut(s)	78, 80, 504
AclWI	GGATC	2 cut(s)	318, 331
AcsI	RAATTY	2 cut(s)	264, 635
AcyI	GRCGYC	1 cut(s)	282
AfaI	GTAC	1 cut(s)	41
AfiI	CCNNNNNNNGG	1 cut(s)	556
AgsI	TTSAA	4 cut(s)	118, 269, 371, 581
AjuI	GAANNNNNNNTTGG	2 cut(s)	255, 287
AluBI	AGCT	4 cut(s)	182, 230, 311, 469
AluI	AGCT	4 cut(s)	182, 230, 311, 469
AlwI	GGATC	2 cut(s)	318, 331
AlwNI	CAGNNNCTG	1 cut(s)	396
Ama87I	CYCGRG	2 cut(s)	22, 54
AoxI	GGCC	1 cut(s)	586
ApeKI	GCWGC	2 cut(s)	544, 572
ApoI	RAATTY	2 cut(s)	264, 635
Asp700I	GAANNNNTTC	1 cut(s)	366
AspA2I	CCTAGG	1 cut(s)	589
AspLEI	GCGC	1 cut(s)	278
AsuC2I	CCSGG	1 cut(s)	532
AsuII	TTCGAA	1 cut(s)	510
AvaI	CYCGRG	2 cut(s)	22, 54
AvrII	CCTAGG	1 cut(s)	589
BaeGI	GKGCMC	1 cut(s)	541
BamHI	GGATCC	1 cut(s)	323
BanI	GGYRCC	1 cut(s)	74
BbvI	GCAGC	2 cut(s)	556, 559
BcgI	CGANNNNNNTGC	1 cut(s)	29
BciVI	GTATCC	1 cut(s)	251
BcnI	CCSGG	1 cut(s)	532
BfaI	CTAG	2 cut(s)	492, 590
BfmI	CTRYAG	1 cut(s)	347
BfuI	GTATCC	1 cut(s)	251
BisI	GCNGC	4 cut(s)	78, 505, 545, 573
BlnI	CCTAGG	1 cut(s)	589
BlsI	GCNGC	4 cut(s)	79, 506, 546, 574
Bme1390I	CCNGG	1 cut(s)	532
BmeT110I	CYCGRG	2 cut(s)	22, 54
BmiI	GGNNCC	3 cut(s)	76, 325, 363
BmrFI	CCNGG	1 cut(s)	532
BmsI	GCATC	1 cut(s)	203
Bpu14I	TTCGAA	1 cut(s)	510
BpuMI	CCSGG	1 cut(s)	532
BsaAI	YACGTR	1 cut(s)	43
BsaBI	GATNNNNATC	1 cut(s)	342
BsaHI	GRCGYC	1 cut(s)	282
BsaJI	CCNNGG	4 cut(s)	55, 78, 327, 589
Bsc4I	CCNNNNNNNGG	1 cut(s)	556
Bse1I	ACTGG	3 cut(s)	299, 316, 429
Bse8I	GATNNNNATC	1 cut(s)	342
BseDI	CCNNGG	4 cut(s)	55, 78, 327, 589
BseGI	GGATG	4 cut(s)	16, 433, 454, 478
BseJI	GATNNNNATC	1 cut(s)	342
BseLI	CCNNNNNNNGG	1 cut(s)	556
BseNI	ACTGG	3 cut(s)	299, 316, 429
BseRI	GAGGAG	1 cut(s)	72
BseSI	GKGCMC	1 cut(s)	541
BseXI	GCAGC	2 cut(s)	556, 559
BsgI	GTGCAG	1 cut(s)	204
Bsh1236I	CGCG	2 cut(s)	80, 276
BshFI	GGCC	1 cut(s)	588
BshNI	GGYRCC	1 cut(s)	74
BsiHKCI	CYCGRG	2 cut(s)	22, 54
BsiSI	CCGG	1 cut(s)	532
BslFI	GGGAC	1 cut(s)	178
BslI	CCNNNNNNNGG	1 cut(s)	556
BsmFI	GGGAC	1 cut(s)	178
BsnI	GGCC	1 cut(s)	588
BsoBI	CYCGRG	2 cut(s)	22, 54
Bsp119I	TTCGAA	1 cut(s)	510
Bsp1286I	GDGCHC	1 cut(s)	541
Bsp143I	GATC	2 cut(s)	323, 343
BspACI	CCGC	3 cut(s)	78, 80, 504
BspANI	GGCC	1 cut(s)	588
BspFNI	CGCG	2 cut(s)	80, 276
BspLI	GGNNCC	3 cut(s)	76, 325, 363
BspMAI	CTGCAG	1 cut(s)	351
BspPI	GGATC	2 cut(s)	318, 331
BspT104I	TTCGAA	1 cut(s)	510
BspT107I	GGYRCC	1 cut(s)	74
BsrI	ACTGG	3 cut(s)	299, 316, 429
BssECI	CCNNGG	4 cut(s)	55, 78, 327, 589
BssMI	GATC	2 cut(s)	323, 343
BssNI	GRCGYC	1 cut(s)	282
BssT1I	CCWWGG	1 cut(s)	589
Bst4CI	ACNGT	2 cut(s)	443, 481
BstACI	GRCGYC	1 cut(s)	282
BstBAI	YACGTR	1 cut(s)	43
BstBI	TTCGAA	1 cut(s)	510
BstDSI	CCRYGG	1 cut(s)	78
BstF5I	GGATG	4 cut(s)	16, 433, 454, 478
BstFNI	CGCG	2 cut(s)	80, 276
BstHHI	GCGC	1 cut(s)	278
BstKTI	GATC	2 cut(s)	326, 346
BstMBI	GATC	2 cut(s)	323, 343
BstMWI	GCNNNNNNNGC	3 cut(s)	227, 236, 501
BstSCI	CCNGG	1 cut(s)	530
BstSFI	CTRYAG	1 cut(s)	347
BstSLI	GKGCMC	1 cut(s)	541
BstUI	CGCG	2 cut(s)	80, 276
BstV1I	GCAGC	2 cut(s)	556, 559
BstX2I	RGATCY	1 cut(s)	323
BstYI	RGATCY	1 cut(s)	323
BsuI	GTATCC	1 cut(s)	251
BsuRI	GGCC	1 cut(s)	588
BtgI	CCRYGG	1 cut(s)	78
BtsCI	GGATG	4 cut(s)	16, 433, 454, 478
BtsI	GCAGTG	1 cut(s)	246
BtsIMutI	CAGTG	2 cut(s)	246, 422
CaiI	CAGNNNCTG	1 cut(s)	396
CfoI	GCGC	1 cut(s)	278
Cfr42I	CCGCGG	1 cut(s)	81
CseI	GACGC	1 cut(s)	290
Csp6I	GTAC	1 cut(s)	40
CviAII	CATG	1 cut(s)	185
CviJI	RGCY	7 cut(s)	182, 230, 311, 469, 507, 560, 588
CviKI_1	RGCY	7 cut(s)	182, 230, 311, 469, 507, 560, 588
CviQI	GTAC	1 cut(s)	40
DpnI	GATC	2 cut(s)	325, 345
DpnII	GATC	2 cut(s)	323, 343
Eco130I	CCWWGG	1 cut(s)	589
Eco88I	CYCGRG	2 cut(s)	22, 54
EcoRI	GAATTC	1 cut(s)	635
EcoT14I	CCWWGG	1 cut(s)	589
ErhI	CCWWGG	1 cut(s)	589
FaeI	CATG	1 cut(s)	188
FaiI	YATR	6 cut(s)	30, 186, 233, 301, 303, 604
FaqI	GGGAC	1 cut(s)	178
FatI	CATG	1 cut(s)	184
FauNDI	CATATG	1 cut(s)	301
Fnu4HI	GCNGC	4 cut(s)	78, 505, 545, 573
FokI	GGATG	4 cut(s)	23, 440, 441, 485
Fsp4HI	GCNGC	4 cut(s)	78, 505, 545, 573
FspBI	CTAG	2 cut(s)	492, 590
GlaI	GCGC	1 cut(s)	277
GluI	GCNGC	4 cut(s)	78, 505, 545, 573
HaeIII	GGCC	1 cut(s)	588
HapII	CCGG	1 cut(s)	532
HgaI	GACGC	1 cut(s)	290
HhaI	GCGC	1 cut(s)	278
Hin1I	GRCGYC	1 cut(s)	282
Hin1II	CATG	1 cut(s)	188
Hin6I	GCGC	1 cut(s)	276
HinP1I	GCGC	1 cut(s)	276
HinfI	GANTC	1 cut(s)	393
HpaII	CCGG	1 cut(s)	532
Hpy166II	GTNNAC	1 cut(s)	85
Hpy188I	TCNGA	5 cut(s)	68, 257, 291, 392, 641
Hpy8I	GTNNAC	1 cut(s)	85
HpyAV	CCTTC	3 cut(s)	105, 203, 371
HpyCH4III	ACNGT	2 cut(s)	443, 481
HpyCH4IV	ACGT	1 cut(s)	42
HpyCH4V	TGCA	3 cut(s)	92, 221, 349
HpyF10VI	GCNNNNNNNGC	3 cut(s)	227, 236, 501
HpySE526I	ACGT	1 cut(s)	42
Hsp92I	GRCGYC	1 cut(s)	282
Hsp92II	CATG	1 cut(s)	188
HspAI	GCGC	1 cut(s)	276
KspI	CCGCGG	1 cut(s)	81
Kzo9I	GATC	2 cut(s)	323, 343
LpnPI	CCDG	9 cut(s)	209, 221, 280, 329, 378, 410, 455, 545, 642
Lsp1109I	GCAGC	2 cut(s)	556, 559
LweI	GCATC	1 cut(s)	203
MaeI	CTAG	2 cut(s)	492, 590
MaeII	ACGT	1 cut(s)	42
MaeIII	GTNAC	1 cut(s)	411
MalI	GATC	2 cut(s)	325, 345
MboI	GATC	2 cut(s)	323, 343
MboII	GAAGA	7 cut(s)	96, 99, 102, 218, 317, 413, 644
MflI	RGATCY	1 cut(s)	323
MhlI	GDGCHC	1 cut(s)	541
MluCI	AATT	3 cut(s)	69, 264, 635
MmeI	TCCRAC	1 cut(s)	235
MnlI	CCTC	4 cut(s)	50, 158, 337, 352
MroXI	GAANNNNTTC	1 cut(s)	366
MseI	TTAA	1 cut(s)	486
MspA1I	CMGCKG	1 cut(s)	80
MspI	CCGG	1 cut(s)	532
MspR9I	CCNGG	1 cut(s)	532
MvnI	CGCG	2 cut(s)	80, 276
MwoI	GCNNNNNNNGC	3 cut(s)	227, 236, 501
NciI	CCSGG	1 cut(s)	532
NdeI	CATATG	1 cut(s)	301
NdeII	GATC	2 cut(s)	323, 343
NlaIII	CATG	1 cut(s)	188
NlaIV	GGNNCC	3 cut(s)	76, 325, 363
NspV	TTCGAA	1 cut(s)	510
PdmI	GAANNNNTTC	1 cut(s)	366
PfeI	GAWTC	1 cut(s)	393
PflMI	CCANNNNNTGG	1 cut(s)	556
PkrI	GCNGC	4 cut(s)	79, 506, 546, 574
Ppu21I	YACGTR	1 cut(s)	43
PsiI	TTATAA	1 cut(s)	30
PspN4I	GGNNCC	3 cut(s)	76, 325, 363
PstI	CTGCAG	1 cut(s)	351
PstNI	CAGNNNCTG	1 cut(s)	396
PsuI	RGATCY	1 cut(s)	323
RsaI	GTAC	1 cut(s)	41
RsaNI	GTAC	1 cut(s)	40
SacII	CCGCGG	1 cut(s)	81
SaqAI	TTAA	1 cut(s)	486
SatI	GCNGC	4 cut(s)	78, 505, 545, 573
Sau3AI	GATC	2 cut(s)	323, 343
ScrFI	CCNGG	1 cut(s)	532
SduI	GDGCHC	1 cut(s)	541
SfaNI	GCATC	1 cut(s)	203
SfcI	CTRYAG	1 cut(s)	347
Sfr303I	CCGCGG	1 cut(s)	81
SfuI	TTCGAA	1 cut(s)	510
SgrBI	CCGCGG	1 cut(s)	81
Sse9I	AATT	3 cut(s)	69, 264, 635
SsiI	CCGC	3 cut(s)	78, 80, 504
SspMI	CTAG	2 cut(s)	492, 590
StyD4I	CCNGG	1 cut(s)	530
StyI	CCWWGG	1 cut(s)	589
TaaI	ACNGT	2 cut(s)	443, 481
TaiI	ACGT	1 cut(s)	45
TaqI	TCGA	2 cut(s)	15, 510
TasI	AATT	3 cut(s)	69, 264, 635
TauI	GCSGC	2 cut(s)	80, 507
TfiI	GAWTC	1 cut(s)	393
Tru1I	TTAA	1 cut(s)	486
Tru9I	TTAA	1 cut(s)	486
TscAI	CASTG	2 cut(s)	246, 429
TseI	GCWGC	2 cut(s)	544, 572
TspDTI	ATGAA	6 cut(s)	63, 87, 318, 390, 444, 489
TspRI	CASTG	2 cut(s)	246, 429
Van91I	CCANNNNNTGG	1 cut(s)	556
XapI	RAATTY	2 cut(s)	264, 635
XmaJI	CCTAGG	1 cut(s)	589
XmnI	GAANNNNTTC	1 cut(s)	366
XspI	CTAG	2 cut(s)	492, 590

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.