MD14G1071200.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0028334
Physical Location & Seq
Reverse (-)
7833316 .. 7835978
2663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1071200.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGGCTAGTACTAGTACTAACCCTACTTACAGTCACAGTGCTCCTCCTGCAGCAGCAGCCTTAGATGGCCAGGAGGGAGGACAACAATATCACGGTTATCAACATGAACATCATCATGGTCGACATGATAAGGAGGACCCGAAGCAGATGATGAGGAATGAGCGTCTTGTGTTGGGGATTCCACCAGGATGGAGGTTTTGTCCTACTGATCAAGAGTTGATTTCTTTTTACTTGACCAGGAAACTCATGAATAACATTTACCACCCTCAGCCCGTCAACAGCAGAATCACTGACCTTCATATCTATGACTTCAACCCCAAGAAACTTGCAGAAAAGTATCAATTACCAGAAGACCCTGAAATGTACTTCTTTACCCCAAGGGACAAAAAGTACCCAAATGGTTGTCGTCCCAGCCGTACAGCTGCTGATGGGTTTTGGAAAGCAACTGGTGGGAATAAAAATATCAAAGAAAGGGGCAAAACTATTGGGTACAAGAACACATTGGTCTTTTTTCTAGGAACACAAAAGAAGGGAACAAAGACTGATTGGATTATGCAAGAATACACGATTAGACAATACAAGCCATCAAATAGGCCAGCTCCAGCAGGTCATTGTGACACCAAGTTGGATAAGTGGGTTCTATGCAAGATCTATGAGCACAAGAAAAGCAAGAAGAACAGAAAAAATGGGAGTGTTTCTGACGATGATGAAGAGGAGATAAATTCATCATCAGCTCTGGTATTGCAAGGTCAAGAAAGTCAAGACCATCCATTGCTTCATAAACCTCTATCGCCACCTAGGAATAACATGAACCATGATGGGCCTTCGACTTCTTATCAAGCGGGACATTACAACATGATCAATAATAATCACAGTGATTATGCATTGAACAACGGATTGATGTCACCATTGGGGTCAACTAATGGCTATGGAGCTCATCAAGATCAGGTGAGACATTTAGCTCCAATGTTACACTTGCCTGGTCAACAAATGCAGCCAATTCCTTTGGTTCATAGCACGGATGTCAACAATCAAGATGCTCATGCATTCAATGGAATCACAACGTACTACAATCAGTCCACTCAATATGGTTTTGGAGATCAATCTGTGCCAATGTACAACAATGCATCACAACTCAACAATAACGGCTATCCATCTAGCATAATTTCAGTTGATACAATGGATCAGTATTGGCTCAATGAGCCACTTACAGACTTTGATTTCTTGTTCGATGCTATCGGTGAAGGAGTTCTTTATGGCATGCCAGATGTATTGCCACTCAGCTCGATGCCTCACATTAACCCTGCGTCGCAGAGCACAAAAGCTGATGAGAAAAGTAGTACCACTCCAACTGATAATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

455

Amino Acids

51.28

Weight (kDa)

6.53

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 61 - 189 4.8e-36 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028334)

Species Orthologous Gene IDs
malus_domestica MD14G1071200.v1.1
prunus_persica Prupe.7G061900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 596
AccI GTMKAC 1 cut(s) 121
AciI CCGC 1 cut(s) 842
AclWI GGATC 1 cut(s) 1191
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 1 cut(s) 721
AfaI GTAC 9 cut(s) 10, 16, 365, 392, 418, 491, 1067, 1118, 1342
AgsI TTSAA 3 cut(s) 313, 889, 1051
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 4 cut(s) 69, 184, 236, 979
AluBI AGCT 7 cut(s) 422, 599, 734, 935, 962, 1284, 1325
AluI AGCT 7 cut(s) 422, 599, 734, 935, 962, 1284, 1325
Alw21I GWGCWC 4 cut(s) 43, 660, 937, 1319
Alw26I GTCTC 1 cut(s) 946
AlwI GGATC 1 cut(s) 1191
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 3 cut(s) 67, 593, 821
ApeKI GCWGC 5 cut(s) 50, 53, 56, 422, 994
ApoI RAATTY 1 cut(s) 721
Asp700I GAANNNNTTC 1 cut(s) 1248
AspA2I CCTAGG 1 cut(s) 797
AspS9I GGNCC 2 cut(s) 136, 821
AsuHPI GGTGA 3 cut(s) 897, 961, 1253
AvaII GGWCC 1 cut(s) 136
AvrII CCTAGG 1 cut(s) 797
BalI TGGCCA 1 cut(s) 69
BanII GRGCYC 1 cut(s) 937
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 4 cut(s) 43, 660, 937, 1319
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 5 cut(s) 62, 65, 68, 409, 1006
BccI CCATC 7 cut(s) 59, 183, 422, 592, 774, 812, 1162
BceAI ACGGC 2 cut(s) 399, 1162
BciT130I CCWGG 4 cut(s) 71, 186, 238, 981
BclI TGATCA 2 cut(s) 208, 858
BcoDI GTCTC 1 cut(s) 946
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 5 cut(s) 6, 12, 515, 798, 1158
BfmI CTRYAG 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 596
BglII AGATCT 1 cut(s) 648
BisI GCNGC 5 cut(s) 51, 54, 57, 423, 995
BlnI CCTAGG 1 cut(s) 797
BlsI GCNGC 5 cut(s) 52, 55, 58, 424, 996
BmcAI AGTACT 2 cut(s) 10, 16
Bme1390I CCNGG 4 cut(s) 71, 186, 238, 981
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 2 cut(s) 136, 821
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 4 cut(s) 71, 186, 238, 981
BmsI GCATC 4 cut(s) 1027, 1136, 1222, 1278
BpiI GAAGAC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 377, 797
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bse1I ACTGG 1 cut(s) 451
Bse3DI GCAATG 1 cut(s) 770
BseBI CCWGG 4 cut(s) 71, 186, 238, 981
BseDI CCNNGG 2 cut(s) 377, 797
BseGI GGATG 3 cut(s) 194, 766, 1027
BseMI GCAATG 1 cut(s) 770
BseMII CTCAG 2 cut(s) 281, 1294
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 2 cut(s) 33, 728
BseXI GCAGC 5 cut(s) 62, 65, 68, 409, 1006
BseYI CCCAGC 1 cut(s) 410
BshFI GGCC 3 cut(s) 69, 595, 823
BsiHKAI GWGCWC 4 cut(s) 43, 660, 937, 1319
BslFI GGGAC 3 cut(s) 393, 395, 858
BsmAI GTCTC 1 cut(s) 946
BsmFI GGGAC 3 cut(s) 393, 395, 858
BsmI GAATGC 1 cut(s) 1046
BsnI GGCC 3 cut(s) 69, 595, 823
Bsp1286I GDGCHC 4 cut(s) 43, 660, 937, 1319
Bsp1407I TGTACA 1 cut(s) 1116
Bsp143I GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
BspACI CCGC 1 cut(s) 842
BspANI GGCC 3 cut(s) 69, 595, 823
BspCNI CTCAG 2 cut(s) 280, 1293
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 138
BspMAI CTGCAG 1 cut(s) 52
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 1 cut(s) 1191
BsrDI GCAATG 1 cut(s) 770
BsrGI TGTACA 1 cut(s) 1116
BsrI ACTGG 1 cut(s) 451
BssECI CCNNGG 2 cut(s) 377, 797
BssMI GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
BssT1I CCWWGG 2 cut(s) 377, 797
Bst2UI CCWGG 4 cut(s) 71, 186, 238, 981
Bst4CI ACNGT 4 cut(s) 32, 38, 95, 875
Bst6I CTCTTC 1 cut(s) 705
BstAUI TGTACA 1 cut(s) 1116
BstC8I GCNNGC 2 cut(s) 597, 1262
BstDEI CTNAG 3 cut(s) 61, 267, 1280
BstF5I GGATG 3 cut(s) 194, 766, 1027
BstKTI GATC 6 cut(s) 211, 651, 861, 946, 1102, 1186
BstMAI GTCTC 1 cut(s) 946
BstMBI GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
BstMWI GCNNNNNNNGC 3 cut(s) 47, 56, 1201
BstNI CCWGG 4 cut(s) 71, 186, 238, 981
BstNSI RCATGY 1 cut(s) 1264
BstSCI CCNGG 4 cut(s) 69, 184, 236, 979
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 5 cut(s) 62, 65, 68, 409, 1006
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 648
BstXI CCANNNNNNTGG 1 cut(s) 189
BstYI RGATCY 1 cut(s) 648
BsuRI GGCC 3 cut(s) 69, 595, 823
BtsCI GGATG 3 cut(s) 194, 766, 1027
BtsIMutI CAGTG 3 cut(s) 43, 288, 880
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 2 cut(s) 597, 1262
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 136, 821
CseI GACGC 2 cut(s) 152, 1296
Csp6I GTAC 9 cut(s) 9, 15, 364, 391, 417, 490, 1066, 1117, 1341
CviAII CATG 9 cut(s) 104, 116, 125, 247, 808, 815, 856, 1043, 1261
CviQI GTAC 9 cut(s) 9, 15, 364, 391, 417, 490, 1066, 1117, 1341
DdeI CTNAG 3 cut(s) 61, 267, 1280
DpnI GATC 6 cut(s) 210, 650, 860, 945, 1101, 1185
DpnII GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 705
EarI CTCTTC 1 cut(s) 705
Ecl136II GAGCTC 1 cut(s) 935
Eco130I CCWWGG 2 cut(s) 377, 797
Eco24I GRGCYC 1 cut(s) 937
Eco47I GGWCC 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 935
EcoICRI GAGCTC 1 cut(s) 935
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 4 cut(s) 69, 184, 236, 979
EcoT14I CCWWGG 2 cut(s) 377, 797
EcoT22I ATGCAT 3 cut(s) 886, 1048, 1129
EcoT38I GRGCYC 1 cut(s) 937
ErhI CCWWGG 2 cut(s) 377, 797
FaeI CATG 9 cut(s) 107, 119, 128, 250, 811, 818, 859, 1046, 1264
FalI AAGNNNNNCTT 2 cut(s) 1236, 1268
FaqI GGGAC 3 cut(s) 393, 395, 858
FatI CATG 9 cut(s) 103, 115, 124, 246, 807, 814, 855, 1042, 1260
FauI CCCGC 1 cut(s) 835
FbaI TGATCA 2 cut(s) 208, 858
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 5 cut(s) 51, 54, 57, 423, 995
FokI GGATG 3 cut(s) 201, 753, 1034
FriOI GRGCYC 1 cut(s) 937
Fsp4HI GCNGC 5 cut(s) 51, 54, 57, 423, 995
FspBI CTAG 5 cut(s) 6, 12, 515, 798, 1158
GluI GCNGC 5 cut(s) 51, 54, 57, 423, 995
GsaI CCCAGC 1 cut(s) 414
GsuI CTGGAG 1 cut(s) 585
HaeIII GGCC 3 cut(s) 69, 595, 823
HgaI GACGC 2 cut(s) 152, 1296
Hin1II CATG 9 cut(s) 107, 119, 128, 250, 811, 818, 859, 1046, 1264
HincII GTYRAC 5 cut(s) 122, 277, 918, 986, 1027
HindII GTYRAC 5 cut(s) 122, 277, 918, 986, 1027
HinfI GANTC 3 cut(s) 178, 285, 1056
HphI GGTGA 3 cut(s) 897, 961, 1253
Hpy166II GTNNAC 6 cut(s) 122, 277, 918, 986, 1027, 1080
Hpy188I TCNGA 1 cut(s) 700
Hpy188III TCNNGA 6 cut(s) 212, 247, 752, 761, 941, 1034
Hpy8I GTNNAC 6 cut(s) 122, 277, 918, 986, 1027, 1080
Hpy99I CGWCG 1 cut(s) 1312
HpyAV CCTTC 4 cut(s) 305, 523, 834, 1238
HpyCH4III ACNGT 4 cut(s) 32, 38, 95, 875
HpyCH4IV ACGT 1 cut(s) 1064
HpyCH4V TGCA 9 cut(s) 50, 329, 556, 645, 745, 884, 994, 1046, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 56, 1201
HpyF3I CTNAG 3 cut(s) 61, 267, 1280
HpySE526I ACGT 1 cut(s) 1064
Hsp92II CATG 9 cut(s) 107, 119, 128, 250, 811, 818, 859, 1046, 1264
Ksp22I TGATCA 2 cut(s) 208, 858
Kzo9I GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
LmnI GCTCC 4 cut(s) 46, 604, 932, 967
Lsp1109I GCAGC 5 cut(s) 62, 65, 68, 409, 1006
LweI GCATC 4 cut(s) 1027, 1136, 1222, 1278
MaeI CTAG 5 cut(s) 6, 12, 515, 798, 1158
MaeII ACGT 1 cut(s) 1064
MaeIII GTNAC 4 cut(s) 32, 614, 903, 969
MalI GATC 6 cut(s) 210, 650, 860, 945, 1101, 1185
MboI GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
MboII GAAGA 3 cut(s) 362, 685, 722
MflI RGATCY 1 cut(s) 648
MhlI GDGCHC 4 cut(s) 43, 660, 937, 1319
MlsI TGGCCA 1 cut(s) 69
MluCI AATT 4 cut(s) 341, 721, 999, 1164
MluNI TGGCCA 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 606
Mox20I TGGCCA 1 cut(s) 69
Mph1103I ATGCAT 3 cut(s) 886, 1048, 1129
MroXI GAANNNNTTC 1 cut(s) 1248
MscI TGGCCA 1 cut(s) 69
MseI TTAA 1 cut(s) 1299
MslI CAYNNNNRTG 3 cut(s) 114, 187, 303
Msp20I TGGCCA 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 422
MspR9I CCNGG 4 cut(s) 71, 186, 238, 981
Mva1269I GAATGC 1 cut(s) 1046
MvaI CCWGG 4 cut(s) 71, 186, 238, 981
MwoI GCNNNNNNNGC 3 cut(s) 47, 56, 1201
NdeII GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
NlaIII CATG 9 cut(s) 107, 119, 128, 250, 811, 818, 859, 1046, 1264
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 3 cut(s) 32, 614, 903
NsiI ATGCAT 3 cut(s) 886, 1048, 1129
NspI RCATGY 1 cut(s) 1264
PaeI GCATGC 1 cut(s) 1264
PagI TCATGA 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PctI GAATGC 1 cut(s) 1046
PdmI GAANNNNTTC 1 cut(s) 1248
PfeI GAWTC 3 cut(s) 178, 285, 1056
PkrI GCNGC 5 cut(s) 52, 55, 58, 424, 996
PpuMI RGGWCCY 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 937
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 4 cut(s) 69, 184, 236, 979
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 4 cut(s) 69, 184, 236, 979
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 2 cut(s) 136, 821
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 648
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 9 cut(s) 10, 16, 365, 392, 418, 491, 1067, 1118, 1342
RsaNI GTAC 9 cut(s) 9, 15, 364, 391, 417, 490, 1066, 1117, 1341
RseI CAYNNNNRTG 3 cut(s) 114, 187, 303
SacI GAGCTC 1 cut(s) 937
SalI GTCGAC 1 cut(s) 120
SaqAI TTAA 1 cut(s) 1299
SatI GCNGC 5 cut(s) 51, 54, 57, 423, 995
Sau3AI GATC 6 cut(s) 208, 648, 858, 943, 1099, 1183
Sau96I GGNCC 2 cut(s) 136, 821
ScaI AGTACT 2 cut(s) 10, 16
ScrFI CCNGG 4 cut(s) 71, 186, 238, 981
SduI GDGCHC 4 cut(s) 43, 660, 937, 1319
SfaNI GCATC 4 cut(s) 1027, 1136, 1222, 1278
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 1 cut(s) 136
SmiMI CAYNNNNRTG 3 cut(s) 114, 187, 303
SpeI ACTAGT 1 cut(s) 11
SphI GCATGC 1 cut(s) 1264
Sse9I AATT 4 cut(s) 341, 721, 999, 1164
SsiI CCGC 1 cut(s) 842
SspMI CTAG 5 cut(s) 6, 12, 515, 798, 1158
SstI GAGCTC 1 cut(s) 937
StyD4I CCNGG 4 cut(s) 69, 184, 236, 979
StyI CCWWGG 2 cut(s) 377, 797
TaaI ACNGT 4 cut(s) 32, 38, 95, 875
TaiI ACGT 1 cut(s) 1067
TaqI TCGA 4 cut(s) 121, 827, 1230, 1286
TasI AATT 4 cut(s) 341, 721, 999, 1164
TatI WGTACW 4 cut(s) 8, 14, 363, 1116
TfiI GAWTC 3 cut(s) 178, 285, 1056
Tru1I TTAA 1 cut(s) 1299
Tru9I TTAA 1 cut(s) 1299
TscAI CASTG 3 cut(s) 43, 295, 880
TseFI GTSAC 3 cut(s) 32, 614, 903
TseI GCWGC 5 cut(s) 50, 53, 56, 422, 994
Tsp45I GTSAC 3 cut(s) 32, 614, 903
TspDTI ATGAA 8 cut(s) 120, 263, 287, 714, 723, 767, 824, 1001
TspGWI ACGGA 2 cut(s) 909, 1034
TspRI CASTG 3 cut(s) 43, 295, 880
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 1 cut(s) 721
XceI RCATGY 1 cut(s) 1264
XmaJI CCTAGG 1 cut(s) 797
XmiI GTMKAC 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 1248
XspI CTAG 5 cut(s) 6, 12, 515, 798, 1158
ZrmI AGTACT 2 cut(s) 10, 16
Zsp2I ATGCAT 3 cut(s) 886, 1048, 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.