Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012128

Physical Location & Seq

Genomic Coordinates Reverse (-)

7976756 .. 7982352

Gene Structure 5597 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1072000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4143 bp

ATGGCTTCTTTGGTATCTTCACCTTTCACCTTACCCAACACAAAAGCAGACCAACTTTCTTCCCTCTCCAGAAAGCAATACTTTCTCCACTCCTTCCTCCCCAAAAATATCACCAATTTGGGCTCTAAGTCCTCCCTCAAAGTGAAATGTGCCATGGGCAATGGCCTCTTCACCCAAACCACCCAGGAAGTCCGCCGCATCGTCCCTGAAAACAAGCAGGGCCTCCCCACTGTCAAAATTGTCTATGTGGTCCTGGAGGCTCAGTACCAATCCTCCCTCACAGCTGCAGTCCAGTCCCTCAACGCCGGCAACCGCTACGCCTCCTTCCAAGTTGTGGGTTATCTAGTCGAAGAGCTTCGTGACGCCGACACCTATAAAATGTTCTGCCAAGACCTTGAGGACGCCAACATTTTCATTGGGTCGTTAATTTTTGTGGAGGAGCTTGCCATCAAAGTGAAGGAGGCTGTGGAGAAGCAGAGAGACAGGATGGATGCAGTGTTGGTGTTTCCGTCAATGCCGGAGGTCATGAGGCTCAACAAGTTGGGATCTTTCAGCATGTCTCAGCTCGGCCAATCGAAAAGCCCATTCTTCCAGCTATTCAAGAGGAAGAAGCAGTCCGCCGGGTTTGCTGACAGCATGCTCAAGCTTGTGAGGACTTTGCCTAAGGTGTTGAAGTACTTGCCGAGTGATAAGGCGCAGGATGCTAGGCTTTACATTCTCAGTTTGCAGTTCTGGCTTGGAGGGTCGCCGGATAACTTGCAGAATTTTGTTAAGATGATATCCGGGTCTTATGTTCCGGCTCTAAAAGGGGAGAAGATTCCGTATTCAGACCCGGTTTTGTTTTTGGATACCGGAATTTGGCACCCTTTGGCACCTTGTATGTATGATGATGTGAAGGAGTACTTGAATTGGTATGGAACTAGGAAGGATGCCAATGAGAAGCTTAAGAGCCCGAATGCGCCTGTGGTCGGGTTGATTCTGCAGAGGAGTCACATTGTTACCGGAGATGAGAGTCATTATGTGGCTGTGATCATGGAACTGGAGGCAAGAGGGGCGAAGGTGATACCGATTTTTGCTGGTGGGCTTGACTTTTCAGGGCCGGTTGAGAGGTTTCTGATTGATCCTGTTACTAAGAAACCATTTATCCATTCTGCAATTTCGCTTACTGGTTTTGCCCTTGTTGGAGGGCCAGCAAGACAGGATCATCCACGGGCCGTTGAAGCTCTGATGAAGCTCGACGTTCCATACATTGTCGCGGTGCCTTTAGTGTTCCAAACAACCGAGGAGTGGTTGAATAGCACTTTGGGTTTGCACCCAATTCAGGTGGCCTTGCAGGTGGCCCTCCCAGAGCTGGACGGAGGCATGGAGCCCATTGTTTTCGCTGGCCGGGATCCTAGAACAGGGAAATCACATGCTCTTCACAAGAGGGTAGAACAGCTCTGCACCAGGGCAATCAGATGGGGTGAACTGAAAAGGAAGACAAAGGCGGAAAAGAAGGTAGCTATCACAGTCTTCAGTTTCCCTCCAGACAAAGGAAATGTTGGAACAGCTGCCTACCTCAATGTTTTCTCCTCAATCTTCGCTGTTCTGCAAGAACTCAAGAGAGATGGTTACAACGTTGAGAACCTTCCAGAGACTTCAGATGCCTTGATTGAGGATGTAATTCATGACAAAGAAGCTCAATTCAGCAGCCCAAATCTGAATGTCGCTTACAAGATGGGGGTCCGTGAATATCAAAGTCTGACTCCATATGCCACTGCGTTGGAGGAAAACTGGGGAAAACCTCCTGGTAACCTCAACTCCGATGGAGAGAATCTATTGGTATATGGAAAACAGTACGGAAATGTCTTCATCGGGGTTCAGCCCACATTTGGCTATGAGGGTGATCCGATGAGGCTGCTGTTCTCCAAATCTGCTAGCCCACATCACGGCTTTGCAGCATACTATTCATTTGTTGAAAAGATTTTCCAAGCTGATGCTGTTCTACATTTCGGTACACATGGTTCACTCGAATTCATGCCTGGAAAACAGGTGGGAATGAGTGATGCCTGCTTCCCGGACAGTCTGATTGGGAACATTCCCAATGTCTATTACTATGCTGCTAACAACCCATCTGAAGCTACGATAGCTAAACGTCGGAGCTATGCCAACACCATCAGCTATCTGACTCCTCCTGCAGAAAATGCTGGACTTTATAAAGGTCTTAAGCAGTTGAGTGAGCTCATCTCCTCCTACCAATCCCTGAGAGACACAGGCCGCGGGTCACAGATCGTTAGCTCAATAATCAGCACAGCCAAGCAGTGCAATCTTGACAAGGACGTAGACCTACCTGAAGAAGGAATGGAAATCTCAGCAAAAGAACGAGATCTTGTGGTTGGAAAGGTGTATTCCAAAATCATGGAGATTGAGTCTCGCCTGTTACCTTGTGGGCTTCACGTCATTGGTGAGCCTCCAACAGCCATGGAAGCAGTTGCAACTCTGGTTAACATTGCTGCACTTGACCGTCCAGAGGAAGGGATTTCTTCCCTCCCATCTATATTAGCTGAGACAGCCGGAAGAGACATAGAGGATATATACAGAAGCAGCGATAAGGGGATATTGAAGGATGTAGAGCTTCTTCGGCAAATAACAGACACATCACGGGGAGCAATTTATGCATTTGTGGAGCGTACGACGAATAGCAAGGGCCAGGTAGTTGACGTTGCTGATAAACTGACCTCAATCCTTGGGTTCGGAATAAACGAACCTTGGGTGCAGTACTTGTCAAACACAAAATTTTACAGGGCTGATAGGGAGAAGCTCAGAACGTTGTTCGTGTTCTTGGCAGAATGCTTGAAGCTAATTGTGGCTGACAATGAGATAGGGAGTTTAAAACAAGCATTGGAGGGTAAATATGTAGAGCCAGGTCCCGGTGGTGACCCAATAAGAAACCCGAAAGTGTTGCCAACAGGAAAGAATATTCATGCACTAGATCCACAATCTATTCCCACAACAGCAGCAATGCAGAGTGCAAAAATTGTGGTGGAGAGGCTGATAGAGAGGCAGAAGATCGATAACGGGGGAAAGTATCCCGAGACTGTAGCACTCGTTTTGTGGGGTACAGACAATATCAAGACTTATGGTGAGTCCTTGGCTCAGGTTTTGTGGATGGTGGGTGTATTACCAGTTGCTGATGCCTTCGGACGGGTCAACCGGGTGGAGATAGTACCTCTTGAAGAGCTTGGAAGACCGAGGATTGATGTTGTTGTCAACTGCTCAGGCGTCTTCAGAGACCTGTTCATCAATCAGATGAATCTGCTCGACCGGGCAGTGAAGATGGTAGCTGAACTAGACGAGCCAGTGGAGCAAAACTTTATCAGGAAGCACGCACTGGAGCAAGCAGAAACCCTAGGAATAGGGGTTCGTGAAGCTGCAACTCGTATCTTCTCCAATGCCTCGGGATCCTATTCCTCCAATATAAATCTTGCTGTGGAGAACTCCTCGTGGAATGATGAGAAGCAGCTGCAAGACATGTATCTAAGCAGGAAGTCATTTGCTTTTGATTCTGATGCCCCTGGTGTAGGCATGGCTGAGAACAGGAAAGTTTTTGAGATGGCTCTGAGCACAGCTGAGGCAACATTCCAAAACCTTGACTCGTCGGAGATCTCACTCACTGATGTGAGTCACTACTTTGACTCGGATCCGACCAATCTGGTGCAAAGCCTAAGGAAGGATGGAAAGAAACCCAGTGCTTACATTGCTGACACCACCACAGCTAATGCCCAGGTTCGCACGCTCTCTGAGACCGTACGTCTTGATGCAAGGACCAAGTTGTTGAACCCTAAGTGGTACGAGGGAATGTTGTCTAGCGGATACGAGGGTGTTCGTGAAATTGAGAAAAGGCTGACTAACACAGTTGGGTGGAGTGCAACTTCTGGCCAAGTTGACAACTGGGTGTACGAAGAGGCCAACACAACTTTCATTCAAGACAAGGAGATGTTGGAAAGGCTCATGAAAACAAATCCAAACTCCTTCAGGAAGCTGGTACAGACATTCCTTGAGGCCAACGGACGTGGTTACTGGGACACTGCAGAGGAGAACATTGAGAAGTTGAAGGAGTTGTACCAAGAAGTTGAAGACAAAATTGAGGGAATCGATCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009526 GO:0009528 GO:0009532 GO:0009536 GO:0009570 GO:0009706 GO:0009941 GO:0010007 GO:0016020 GO:0016851 GO:0016874 GO:0019866 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032991 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0051002 GO:0051003 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

CobN-Mg_chel DUF3479

Protein Analysis

1381

Amino Acids

153.32

Weight (kDa)

5.76

Isoelectric Point (pI)

40.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3479	PF11965	79 - 239	6.5e-48	Domain of unknown function (DUF3479)
CobN-Mg_chel	PF02514	243 - 1360	0.0	CobN/Magnesium Chelatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13630 AT5G13630
fragaria_vesca	FvH4_6g23800
malus_domestica	MD12G1073700.v1.1 MD14G1072000.v1.1
prunus_persica	Prupe.7G061100_v2.0.a1 Prupe.7G061100_v2.0.a1
pyrus_communis	pycom12g06370 pycom14g06180
rosa_chinensis	RchiOBHm_Chr3g0482331
rosa_laevigata	RLG00000023358
rosa_multiflora	Rmu_sc0000135.1_g000006
rosa_roxburghii	Rroxscaffold_6G00399420
rosa_rugosa	Rorug03G0195800
rosa_samantha	Rh3AG247500 Rh3BG282000 Rh3CG279000 Rh3DG274800
rosa_wichuraiana	Rw3G022190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2196
AarI	CACCTGC	1 cut(s)	1324
Acc36I	ACCTGC	1 cut(s)	1324
AccB1I	GGYRCC	3 cut(s)	861, 871, 1258
AccB7I	CCANNNNNTGG	1 cut(s)	334
AccI	GTMKAC	1 cut(s)	2322
AccII	CGCG	2 cut(s)	1256, 2259
AciI	CCGC	9 cut(s)	193, 196, 313, 618, 1256, 1487, 2257, 2259, 3849
AclI	AACGTT	2 cut(s)	1617, 2807
AcoI	YGGCCR	3 cut(s)	568, 1384, 3916
AcsI	RAATTY	4 cut(s)	763, 855, 2012, 2774
AcuI	CTGAAG	6 cut(s)	1498, 1623, 2136, 2352, 3250, 3997
AcyI	GRCGYC	3 cut(s)	363, 402, 3261
AflII	CTTAAG	2 cut(s)	944, 2204
AflIII	ACRYGT	1 cut(s)	3510
AhdI	GACNNNNNGTC	1 cut(s)	3789
AjiI	CACGTC	2 cut(s)	2437, 4052
AjnI	CCWGG	9 cut(s)	183, 252, 1445, 1786, 2020, 2688, 2902, 3553, 3762
AjuI	GAANNNNNNNTTGG	4 cut(s)	1286, 1318, 3941, 3973
AleI	CACNNNNGTG	1 cut(s)	3656
AloI	GAACNNNNNNTCC	2 cut(s)	3384, 3416
Alw21I	GWGCWC	2 cut(s)	2223, 3605
AlwNI	CAGNNNCTG	1 cut(s)	3170
Ama87I	CYCGRG	2 cut(s)	3071, 3436
ApoI	RAATTY	4 cut(s)	763, 855, 2012, 2774
Asp700I	GAANNNNTTC	1 cut(s)	354
AspA2I	CCTAGG	1 cut(s)	3388
AspLEI	GCGC	2 cut(s)	697, 961
AsuC2I	CCSGG	8 cut(s)	622, 784, 833, 1388, 2057, 2910, 3194, 3305
AsuNHI	GCTAGC	1 cut(s)	1916
AvaI	CYCGRG	2 cut(s)	3071, 3436
AvaII	GGWCC	4 cut(s)	250, 1723, 2906, 3804
AvrII	CCTAGG	1 cut(s)	3388
AxyI	CCTNAGG	2 cut(s)	663, 3704
BaeI	ACNNNNGTAYC	2 cut(s)	3844, 3877
BalI	TGGCCA	1 cut(s)	3918
BamHI	GGATCC	3 cut(s)	1390, 3440, 3679
BanI	GGYRCC	3 cut(s)	861, 871, 1258
BanII	GRGCYC	4 cut(s)	125, 953, 1371, 2223
BarI	GAAGNNNNNNTAC	4 cut(s)	2601, 2633, 4085, 4117
BauI	CACGAG	1 cut(s)	3481
BbsI	GAAGAC	6 cut(s)	1484, 1504, 1840, 3232, 3256, 4122
Bbv12I	GWGCWC	2 cut(s)	2223, 3605
BbvCI	CCTCAGC	1 cut(s)	3609
BceAI	ACGGC	2 cut(s)	1199, 1945
BcgI	CGANNNNNNTGC	4 cut(s)	1879, 1913, 2923, 2957
BciT130I	CCWGG	9 cut(s)	185, 254, 1447, 1788, 2022, 2690, 2904, 3555, 3764
BciVI	GTATCC	3 cut(s)	841, 3078, 3845
BclI	TGATCA	1 cut(s)	1029
BcnI	CCSGG	8 cut(s)	622, 784, 833, 1388, 2057, 2910, 3194, 3305
BfaI	CTAG	9 cut(s)	344, 705, 921, 1395, 1917, 2969, 3329, 3389, 3846
BfmI	CTRYAG	5 cut(s)	285, 980, 2175, 3078, 4068
BfrI	CTTAAG	2 cut(s)	944, 2204
BfuAI	ACCTGC	1 cut(s)	1324
BfuI	GTATCC	3 cut(s)	841, 3078, 3845
BglII	AGATCT	2 cut(s)	2365, 3642
BlnI	CCTAGG	1 cut(s)	3388
BmcAI	AGTACT	3 cut(s)	677, 902, 2759
Bme18I	GGWCC	4 cut(s)	250, 1723, 2906, 3804
BmeRI	GACNNNNNGTC	1 cut(s)	3789
BmeT110I	CYCGRG	2 cut(s)	3071, 3436
BmgBI	CACGTC	2 cut(s)	2437, 4052
BmiI	GGNNCC	9 cut(s)	863, 873, 1260, 1368, 1392, 1724, 2908, 3442, 3681
BmrI	ACTGGG	4 cut(s)	1783, 3720, 3940, 4069
BmtI	GCTAGC	1 cut(s)	1920
BmuI	ACTGGG	4 cut(s)	1783, 3720, 3940, 4069
BpiI	GAAGAC	6 cut(s)	1484, 1504, 1840, 3232, 3256, 4122
BplI	GAGNNNNNCTC	4 cut(s)	2210, 2242, 3464, 3496
BpmI	CTGGAG	5 cut(s)	52, 275, 1061, 1509, 3392
Bpu10I	CCTNAGC	3 cut(s)	3135, 3256, 3609
BpuEI	CTTGAG	4 cut(s)	416, 626, 1583, 4058
BpuMI	CCSGG	8 cut(s)	622, 784, 833, 1388, 2057, 2910, 3194, 3305
Bsa29I	ATCGAT	2 cut(s)	3051, 4134
BsaHI	GRCGYC	3 cut(s)	363, 402, 3261
BsaI	GGTCTC	2 cut(s)	3264, 3776
BsaWI	WCCGGW	2 cut(s)	851, 1001
BsaXI	ACNNNNNCTCC	8 cut(s)	248, 257, 278, 287, 1784, 1814, 2393, 2423
Bse118I	RCCGGY	2 cut(s)	305, 1099
Bse21I	CCTNAGG	2 cut(s)	663, 3704
Bse3DI	GCAATG	4 cut(s)	166, 2487, 3006, 3735
BseBI	CCWGG	9 cut(s)	185, 254, 1447, 1788, 2022, 2690, 2904, 3555, 3764
BseCI	ATCGAT	2 cut(s)	3051, 4134
BseGI	GGATG	9 cut(s)	492, 496, 706, 934, 1204, 1663, 2611, 3153, 3718
BseMI	GCAATG	4 cut(s)	166, 2487, 3006, 3735
BseRI	GAGGAG	8 cut(s)	452, 1000, 1298, 1561, 2160, 2218, 3469, 4088
BsgI	GTGCAG	3 cut(s)	1426, 2478, 2774
Bsh1236I	CGCG	2 cut(s)	1256, 2259
Bsh1285I	CGRYCG	2 cut(s)	3304, 4138
BshNI	GGYRCC	3 cut(s)	861, 871, 1258
BshVI	ATCGAT	2 cut(s)	3051, 4134
BsiEI	CGRYCG	2 cut(s)	3304, 4138
BsiHKAI	GWGCWC	2 cut(s)	2223, 3605
BsiHKCI	CYCGRG	2 cut(s)	3071, 3436
BsiWI	CGTACG	2 cut(s)	2669, 3787
BslFI	GGGAC	4 cut(s)	188, 280, 2892, 4076
BsmFI	GGGAC	4 cut(s)	188, 280, 2892, 4076
BsmI	GAATGC	2 cut(s)	961, 2834
Bso31I	GGTCTC	2 cut(s)	3264, 3776
BsoBI	CYCGRG	2 cut(s)	3071, 3436
Bsp1286I	GDGCHC	5 cut(s)	125, 953, 1371, 2223, 3605
Bsp19I	CCATGG	2 cut(s)	153, 2460
BspACI	CCGC	9 cut(s)	193, 196, 313, 618, 1256, 1487, 2257, 2259, 3849
BspDI	ATCGAT	2 cut(s)	3051, 4134
BspFNI	CGCG	2 cut(s)	1256, 2259
BspHI	TCATGA	3 cut(s)	525, 1666, 3990
BspLI	GGNNCC	9 cut(s)	863, 873, 1260, 1368, 1392, 1724, 2908, 3442, 3681
BspMAI	CTGCAG	4 cut(s)	289, 984, 2179, 4072
BspMI	ACCTGC	1 cut(s)	1324
BspOI	GCTAGC	1 cut(s)	1920
BspQI	GCTCTTC	3 cut(s)	345, 1422, 3210
BspT107I	GGYRCC	3 cut(s)	861, 871, 1258
BspTI	CTTAAG	2 cut(s)	944, 2204
BspTNI	GGTCTC	2 cut(s)	3264, 3776
BsrDI	GCAATG	4 cut(s)	166, 2487, 3006, 3735
BsrFI	RCCGGY	2 cut(s)	305, 1099
BssAI	RCCGGY	2 cut(s)	305, 1099
BssNI	GRCGYC	3 cut(s)	363, 402, 3261
BssSI	CACGAG	1 cut(s)	3481
BssT1I	CCWWGG	6 cut(s)	153, 2460, 2725, 2747, 3129, 3388
Bst2BI	CACGAG	1 cut(s)	3481
Bst2UI	CCWGG	9 cut(s)	185, 254, 1447, 1788, 2022, 2690, 2904, 3555, 3764
Bst4CI	ACNGT	8 cut(s)	232, 1510, 1836, 2063, 2504, 3079, 3787, 3895
Bst6I	CTCTTC	6 cut(s)	173, 345, 1422, 2551, 3210, 3936
BstACI	GRCGYC	3 cut(s)	363, 402, 3261
BstAFI	CTTAAG	2 cut(s)	944, 2204
BstAPI	GCANNNNNTGC	2 cut(s)	2183, 2654
BstDSI	CCRYGG	4 cut(s)	153, 1208, 2257, 2460
BstEII	GGTNACC	2 cut(s)	1790, 2915
BstENI	CCTNNNNNAGG	4 cut(s)	1398, 2334, 3558, 3707
BstF5I	GGATG	9 cut(s)	492, 496, 706, 934, 1204, 1663, 2611, 3153, 3718
BstFNI	CGCG	2 cut(s)	1256, 2259
BstHHI	GCGC	2 cut(s)	697, 961
BstMCI	CGRYCG	2 cut(s)	3304, 4138
BstNI	CCWGG	9 cut(s)	185, 254, 1447, 1788, 2022, 2690, 2904, 3555, 3764
BstNSI	RCATGY	4 cut(s)	559, 640, 1415, 3514
BstPI	GGTNACC	2 cut(s)	1790, 2915
BstSFI	CTRYAG	5 cut(s)	285, 980, 2175, 3078, 4068
BstUI	CGCG	2 cut(s)	1256, 2259
BstV2I	GAAGAC	6 cut(s)	1484, 1504, 1840, 3232, 3256, 4122
BstX2I	RGATCY	7 cut(s)	545, 1390, 2365, 2971, 3440, 3642, 3679
BstXI	CCANNNNNNTGG	2 cut(s)	1762, 2398
BstYI	RGATCY	7 cut(s)	545, 1390, 2365, 2971, 3440, 3642, 3679
Bsu15I	ATCGAT	2 cut(s)	3051, 4134
Bsu36I	CCTNAGG	2 cut(s)	663, 3704
BsuI	GTATCC	3 cut(s)	841, 3078, 3845
BsuTUI	ATCGAT	2 cut(s)	3051, 4134
BtgI	CCRYGG	4 cut(s)	153, 1208, 2257, 2460
BtrI	CACGTC	2 cut(s)	2437, 4052
BtsCI	GGATG	9 cut(s)	492, 496, 706, 934, 1204, 1663, 2611, 3153, 3718
BtsI	GCAGTG	5 cut(s)	501, 1755, 2306, 3315, 4065
BveI	ACCTGC	1 cut(s)	1324
CaiI	CAGNNNCTG	1 cut(s)	3170
CciI	TCATGA	3 cut(s)	525, 1666, 3990
CfoI	GCGC	2 cut(s)	697, 961
Cfr10I	RCCGGY	2 cut(s)	305, 1099
Cfr42I	CCGCGG	1 cut(s)	2260
ClaI	ATCGAT	2 cut(s)	3051, 4134
CseI	GACGC	3 cut(s)	371, 410, 3250
CspCI	CAANNNNNGTGG	6 cut(s)	1305, 1340, 3000, 3035, 4033, 4068
DraI	TTTAAA	1 cut(s)	2871
DriI	GACNNNNNGTC	1 cut(s)	3789
EaeI	YGGCCR	3 cut(s)	568, 1384, 3916
Eam1104I	CTCTTC	6 cut(s)	173, 345, 1422, 2551, 3210, 3936
Eam1105I	GACNNNNNGTC	1 cut(s)	3789
EarI	CTCTTC	6 cut(s)	173, 345, 1422, 2551, 3210, 3936
EciI	GGCGGA	3 cut(s)	182, 607, 1502
Ecl136II	GAGCTC	1 cut(s)	2221
Eco130I	CCWWGG	6 cut(s)	153, 2460, 2725, 2747, 3129, 3388
Eco24I	GRGCYC	4 cut(s)	125, 953, 1371, 2223
Eco31I	GGTCTC	2 cut(s)	3264, 3776
Eco32I	GATATC	1 cut(s)	780
Eco47I	GGWCC	4 cut(s)	250, 1723, 2906, 3804
Eco53kI	GAGCTC	1 cut(s)	2221
Eco57I	CTGAAG	6 cut(s)	1498, 1623, 2136, 2352, 3250, 3997
Eco81I	CCTNAGG	2 cut(s)	663, 3704
Eco88I	CYCGRG	2 cut(s)	3071, 3436
Eco91I	GGTNACC	2 cut(s)	1790, 2915
EcoICRI	GAGCTC	1 cut(s)	2221
EcoNI	CCTNNNNNAGG	4 cut(s)	1398, 2334, 3558, 3707
EcoO109I	RGGNCCY	2 cut(s)	220, 2906
EcoO65I	GGTNACC	2 cut(s)	1790, 2915
EcoRI	GAATTC	1 cut(s)	2012
EcoRII	CCWGG	9 cut(s)	183, 252, 1445, 1786, 2020, 2688, 2902, 3553, 3762
EcoRV	GATATC	1 cut(s)	780
EcoT14I	CCWWGG	6 cut(s)	153, 2460, 2725, 2747, 3129, 3388
EcoT22I	ATGCAT	1 cut(s)	2659
EcoT38I	GRGCYC	4 cut(s)	125, 953, 1371, 2223
ErhI	CCWWGG	6 cut(s)	153, 2460, 2725, 2747, 3129, 3388
FalI	AAGNNNNNCTT	4 cut(s)	65, 97, 887, 919
FaqI	GGGAC	4 cut(s)	188, 280, 2892, 4076
FauI	CCCGC	1 cut(s)	2252
FauNDI	CATATG	1 cut(s)	1750
FbaI	TGATCA	1 cut(s)	1029
FblI	GTMKAC	1 cut(s)	2322
FokI	GGATG	9 cut(s)	499, 503, 713, 941, 1191, 1670, 2618, 3160, 3725
FriOI	GRGCYC	4 cut(s)	125, 953, 1371, 2223
FspBI	CTAG	9 cut(s)	344, 705, 921, 1395, 1917, 2969, 3329, 3389, 3846
GlaI	GCGC	2 cut(s)	696, 960
GsuI	CTGGAG	5 cut(s)	52, 275, 1061, 1509, 3392
HgaI	GACGC	3 cut(s)	371, 410, 3250
HhaI	GCGC	2 cut(s)	697, 961
Hin1I	GRCGYC	3 cut(s)	363, 402, 3261
Hin6I	GCGC	2 cut(s)	695, 959
HinP1I	GCGC	2 cut(s)	695, 959
HincII	GTYRAC	5 cut(s)	2485, 2698, 3190, 3250, 3925
HindII	GTYRAC	5 cut(s)	2485, 2698, 3190, 3250, 3925
HindIII	AAGCTT	2 cut(s)	644, 941
HpaI	GTTAAC	1 cut(s)	2485
Hpy99I	CGWCG	4 cut(s)	1241, 2139, 2677, 3640
HpyCH4III	ACNGT	8 cut(s)	232, 1510, 1836, 2063, 2504, 3079, 3787, 3895
HpyCH4IV	ACGT	9 cut(s)	1239, 1617, 2134, 2319, 2436, 2700, 2807, 3790, 4051
HpySE526I	ACGT	9 cut(s)	1239, 1617, 2134, 2319, 2436, 2700, 2807, 3790, 4051
Hsp92I	GRCGYC	3 cut(s)	363, 402, 3261
HspAI	GCGC	2 cut(s)	695, 959
KroI	GCCGGC	1 cut(s)	305
KroNI	GCCGGC	1 cut(s)	307
Ksp22I	TGATCA	1 cut(s)	1029
KspAI	GTTAAC	1 cut(s)	2485
KspI	CCGCGG	1 cut(s)	2260
LguI	GCTCTTC	3 cut(s)	345, 1422, 3210
LmnI	GCTCC	7 cut(s)	439, 1366, 2139, 2645, 2665, 3343, 3373
MaeI	CTAG	9 cut(s)	344, 705, 921, 1395, 1917, 2969, 3329, 3389, 3846
MaeII	ACGT	9 cut(s)	1239, 1617, 2134, 2319, 2436, 2700, 2807, 3790, 4051
MflI	RGATCY	7 cut(s)	545, 1390, 2365, 2971, 3440, 3642, 3679
MhlI	GDGCHC	5 cut(s)	125, 953, 1371, 2223, 3605
MlsI	TGGCCA	1 cut(s)	3918
MluNI	TGGCCA	1 cut(s)	3918
MlyI	GAGTC	9 cut(s)	997, 1021, 1738, 2161, 2417, 3134, 3626, 3668, 3670
MmeI	TCCRAC	9 cut(s)	1162, 1522, 1743, 2117, 2356, 2477, 3618, 3707, 3960
Mox20I	TGGCCA	1 cut(s)	3918
Mph1103I	ATGCAT	1 cut(s)	2659
MroNI	GCCGGC	1 cut(s)	305
MroXI	GAANNNNTTC	1 cut(s)	354
MscI	TGGCCA	1 cut(s)	3918
MseI	TTAA	6 cut(s)	425, 771, 945, 2205, 2484, 2870
MslI	CAYNNNNRTG	2 cut(s)	452, 3656
Msp20I	TGGCCA	1 cut(s)	3918
MspA1I	CMGCKG	5 cut(s)	284, 1550, 2259, 3502, 3608
MspCI	CTTAAG	2 cut(s)	944, 2204
Mva1269I	GAATGC	2 cut(s)	961, 2834
MvaI	CCWGG	9 cut(s)	185, 254, 1447, 1788, 2022, 2690, 2904, 3555, 3764
MvnI	CGCG	2 cut(s)	1256, 2259
NaeI	GCCGGC	1 cut(s)	307
NciI	CCSGG	8 cut(s)	622, 784, 833, 1388, 2057, 2910, 3194, 3305
NcoI	CCATGG	2 cut(s)	153, 2460
NdeI	CATATG	1 cut(s)	1750
NgoMIV	GCCGGC	1 cut(s)	305
NheI	GCTAGC	1 cut(s)	1916
NlaIV	GGNNCC	9 cut(s)	863, 873, 1260, 1368, 1392, 1724, 2908, 3442, 3681
NmeAIII	GCCGAG	2 cut(s)	546, 708
NmuCI	GTSAC	5 cut(s)	359, 989, 2262, 2915, 3662
NsiI	ATGCAT	1 cut(s)	2659
NspI	RCATGY	4 cut(s)	559, 640, 1415, 3514
OliI	CACNNNNGTG	1 cut(s)	3656
PaeI	GCATGC	1 cut(s)	640
PagI	TCATGA	3 cut(s)	525, 1666, 3990
PaqCI	CACCTGC	1 cut(s)	1324
PciI	ACATGT	1 cut(s)	3510
PciSI	GCTCTTC	3 cut(s)	345, 1422, 3210
PcsI	WCGNNNNNNNCGW	1 cut(s)	2739
PctI	GAATGC	2 cut(s)	961, 2834
PdiI	GCCGGC	1 cut(s)	307
PdmI	GAANNNNTTC	1 cut(s)	354
PfeI	GAWTC	6 cut(s)	817, 976, 1813, 3292, 3542, 4131
Pfl23II	CGTACG	2 cut(s)	2669, 3787
PflMI	CCANNNNNTGG	1 cut(s)	334
PfoI	TCCNGGA	2 cut(s)	252, 2055
Ple19I	CGATCG	1 cut(s)	4138
PleI	GAGTC	9 cut(s)	996, 1020, 1738, 2161, 2416, 3133, 3626, 3668, 3669
PpsI	GAGTC	9 cut(s)	996, 1020, 1738, 2161, 2416, 3133, 3626, 3668, 3669
PpuMI	RGGWCCY	1 cut(s)	2906
PscI	ACATGT	1 cut(s)	3510
PsiI	TTATAA	1 cut(s)	2196
Psp124BI	GAGCTC	1 cut(s)	2223
Psp1406I	AACGTT	2 cut(s)	1617, 2807
Psp5II	RGGWCCY	1 cut(s)	2906
Psp6I	CCWGG	9 cut(s)	183, 252, 1445, 1786, 2020, 2688, 2902, 3553, 3762
PspEI	GGTNACC	2 cut(s)	1790, 2915
PspGI	CCWGG	9 cut(s)	183, 252, 1445, 1786, 2020, 2688, 2902, 3553, 3762
PspLI	CGTACG	2 cut(s)	2669, 3787
PspN4I	GGNNCC	9 cut(s)	863, 873, 1260, 1368, 1392, 1724, 2908, 3442, 3681
PspPPI	RGGWCCY	1 cut(s)	2906
PsrI	GAACNNNNNNTAC	2 cut(s)	1987, 2019
PstI	CTGCAG	4 cut(s)	289, 984, 2179, 4072
PstNI	CAGNNNCTG	1 cut(s)	3170
PsuI	RGATCY	7 cut(s)	545, 1390, 2365, 2971, 3440, 3642, 3679
PvuI	CGATCG	1 cut(s)	4138
PvuII	CAGCTG	4 cut(s)	284, 1550, 3502, 3608
RseI	CAYNNNNRTG	2 cut(s)	452, 3656
SacI	GAGCTC	1 cut(s)	2223
SacII	CCGCGG	1 cut(s)	2260
SapI	GCTCTTC	3 cut(s)	345, 1422, 3210
SaqAI	TTAA	6 cut(s)	425, 771, 945, 2205, 2484, 2870
ScaI	AGTACT	3 cut(s)	677, 902, 2759
SchI	GAGTC	9 cut(s)	997, 1021, 1738, 2161, 2417, 3134, 3626, 3668, 3670
SduI	GDGCHC	5 cut(s)	125, 953, 1371, 2223, 3605
SfcI	CTRYAG	5 cut(s)	285, 980, 2175, 3078, 4068
Sfr303I	CCGCGG	1 cut(s)	2260
SgrBI	CCGCGG	1 cut(s)	2260
SinI	GGWCC	4 cut(s)	250, 1723, 2906, 3804
SmiMI	CAYNNNNRTG	2 cut(s)	452, 3656
SmlI	CTYRAG	6 cut(s)	395, 641, 944, 1598, 2204, 4037
SmoI	CTYRAG	6 cut(s)	395, 641, 944, 1598, 2204, 4037
SphI	GCATGC	1 cut(s)	640
SsiI	CCGC	9 cut(s)	193, 196, 313, 618, 1256, 1487, 2257, 2259, 3849
SspI	AATATT	1 cut(s)	2959
SspMI	CTAG	9 cut(s)	344, 705, 921, 1395, 1917, 2969, 3329, 3389, 3846
SstI	GAGCTC	1 cut(s)	2223
StyI	CCWWGG	6 cut(s)	153, 2460, 2725, 2747, 3129, 3388
TaaI	ACNGT	8 cut(s)	232, 1510, 1836, 2063, 2504, 3079, 3787, 3895
TaiI	ACGT	9 cut(s)	1242, 1620, 2137, 2322, 2439, 2703, 2810, 3793, 4054
TaqI	TCGA	7 cut(s)	348, 575, 1236, 2010, 3051, 3300, 4134
TaqII	GACCGA	1 cut(s)	3244
TatI	WGTACW	3 cut(s)	675, 900, 2757
TauI	GCSGC	2 cut(s)	198, 2259
TfiI	GAWTC	6 cut(s)	817, 976, 1813, 3292, 3542, 4131
Tru1I	TTAA	6 cut(s)	425, 771, 945, 2205, 2484, 2870
Tru9I	TTAA	6 cut(s)	425, 771, 945, 2205, 2484, 2870
TseFI	GTSAC	5 cut(s)	359, 989, 2262, 2915, 3662
Tsp45I	GTSAC	5 cut(s)	359, 989, 2262, 2915, 3662
TspGWI	ACGGA	6 cut(s)	498, 810, 1371, 1715, 1854, 4062
Van91I	CCANNNNNTGG	1 cut(s)	334
Vha464I	CTTAAG	2 cut(s)	944, 2204
VpaK11BI	GGWCC	4 cut(s)	250, 1723, 2906, 3804
XagI	CCTNNNNNAGG	4 cut(s)	1398, 2334, 3558, 3707
XapI	RAATTY	4 cut(s)	763, 855, 2012, 2774
XceI	RCATGY	4 cut(s)	559, 640, 1415, 3514
XmaJI	CCTAGG	1 cut(s)	3388
XmiI	GTMKAC	1 cut(s)	2322
XmnI	GAANNNNTTC	1 cut(s)	354
XspI	CTAG	9 cut(s)	344, 705, 921, 1395, 1917, 2969, 3329, 3389, 3846
ZrmI	AGTACT	3 cut(s)	677, 902, 2759
Zsp2I	ATGCAT	1 cut(s)	2659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.