MD14G1216400.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0028338
Physical Location & Seq
Forward (+)
29991996 .. 29992538
543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1216400.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCTGCTGTGACTAGCAAGCTCCTTCTTCTCATTGCAACTCTTGCATTAACGTGTTGTACCCCTACCACAGAGGGCTTGAAACGCAGCATCACCAGAATGCAGTTCTACATGCACGACGTCGTAGGCGGGCCAAACCCGACCGCAGTAAGAGTTGCAAGCCGGTTGTCCAACTACACAGGCACGGACCCAATCGCCGCCCTTTTCGGGTCAGTTTACATGATGGACAATCCACTCACGGCCACGCCTGACCCCAAGTCCGCCCTGATGGGACGGGCGCAAGGGATATACGCCATGGCCTCGCAACGGGACGAGTTCAGCCTCCTCATGACACTAACGTATGGGTTCACTGGTGGAGCTTACAACGGCAGCACATTTAGCGTGGTGGGTCGGAACCCGGTGATGAGCGAAGAAAGGGAGATGCCGGTCCTTGGAGGCACTGGCATGTTTAGGCTTGCTTGCGGATTTTGCATTGCAAGAACTCATTCTGTGGACCAAATGAACGCAATTATTGGATACAATGTCACGCTCATACACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

181

Amino Acids

19.38

Weight (kDa)

8.39

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 32 - 177 3.1e-50 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028338)

Species Orthologous Gene IDs
malus_domestica MD14G1216400.v1.1
pyrus_communis pycom14g17920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
AciI CCGC 5 cut(s) 129, 144, 198, 261, 462
AcoI YGGCCR 1 cut(s) 240
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 3 cut(s) 207, 306, 431
AflIII ACRYGT 1 cut(s) 53
AgsI TTSAA 1 cut(s) 82
AhdI GACNNNNNGTC 1 cut(s) 256
AluBI AGCT 2 cut(s) 22, 359
AluI AGCT 2 cut(s) 22, 359
AoxI GGCC 3 cut(s) 131, 240, 297
ApeKI GCWGC 3 cut(s) 5, 87, 369
Asp700I GAANNNNTTC 1 cut(s) 484
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 4 cut(s) 131, 187, 427, 493
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 2 cut(s) 85, 412
AvaII GGWCC 3 cut(s) 187, 427, 493
BbvI GCAGC 2 cut(s) 99, 381
BccI CCATC 2 cut(s) 217, 262
BceAI ACGGC 2 cut(s) 255, 382
BciVI GTATCC 1 cut(s) 509
BcnI CCSGG 1 cut(s) 398
BfaI CTAG 1 cut(s) 15
BfuI GTATCC 1 cut(s) 509
BisI GCNGC 4 cut(s) 6, 88, 198, 370
BlsI GCNGC 4 cut(s) 7, 89, 199, 371
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 3 cut(s) 187, 427, 493
BmeRI GACNNNNNGTC 1 cut(s) 256
BmgT120I GGNCC 4 cut(s) 131, 187, 427, 493
BmiI GGNNCC 2 cut(s) 189, 395
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 2 cut(s) 99, 411
BpuMI CCSGG 1 cut(s) 398
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 294, 430
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 306, 431
Bse118I RCCGGY 2 cut(s) 162, 424
Bse1I ACTGG 2 cut(s) 355, 445
Bse3DI GCAATG 2 cut(s) 33, 471
BseDI CCNNGG 2 cut(s) 294, 430
BseLI CCNNNNNNNGG 3 cut(s) 207, 306, 431
BseMI GCAATG 2 cut(s) 33, 471
BseNI ACTGG 2 cut(s) 355, 445
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 2 cut(s) 99, 381
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 3 cut(s) 133, 242, 299
BsiEI CGRYCG 1 cut(s) 144
BsiSI CCGG 3 cut(s) 163, 398, 425
BslFI GGGAC 2 cut(s) 285, 323
BslI CCNNNNNNNGG 3 cut(s) 207, 306, 431
BsmFI GGGAC 2 cut(s) 285, 323
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 3 cut(s) 133, 242, 299
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 5 cut(s) 129, 144, 198, 261, 462
BspANI GGCC 3 cut(s) 133, 242, 299
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 2 cut(s) 189, 395
BsrDI GCAATG 2 cut(s) 33, 471
BsrFI RCCGGY 2 cut(s) 162, 424
BsrI ACTGG 2 cut(s) 355, 445
BssAI RCCGGY 2 cut(s) 162, 424
BssECI CCNNGG 2 cut(s) 294, 430
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 2 cut(s) 294, 430
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 44
BstC8I GCNNGC 5 cut(s) 20, 131, 160, 456, 460
BstDSI CCRYGG 1 cut(s) 294
BstHHI GCGC 1 cut(s) 280
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 4 cut(s) 44, 84, 378, 468
BstNSI RCATGY 2 cut(s) 115, 448
BstSCI CCNGG 1 cut(s) 396
BstV1I GCAGC 2 cut(s) 99, 381
BsuI GTATCC 1 cut(s) 509
BsuRI GGCC 3 cut(s) 133, 242, 299
BtgI CCRYGG 1 cut(s) 294
BtsIMutI CAGTG 2 cut(s) 348, 438
Cac8I GCNNGC 5 cut(s) 20, 131, 160, 456, 460
CciI TCATGA 1 cut(s) 327
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 2 cut(s) 162, 424
Cfr13I GGNCC 4 cut(s) 131, 187, 427, 493
Csp6I GTAC 1 cut(s) 60
CviAII CATG 5 cut(s) 112, 220, 295, 328, 445
CviQI GTAC 1 cut(s) 60
DriI GACNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 240
Eam1105I GACNNNNNGTC 1 cut(s) 256
EciI GGCGGA 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 294, 430
Eco47I GGWCC 3 cut(s) 187, 427, 493
EcoT14I CCWWGG 2 cut(s) 294, 430
ErhI CCWWGG 2 cut(s) 294, 430
FaeI CATG 5 cut(s) 115, 223, 298, 331, 448
FaiI YATR 8 cut(s) 113, 221, 289, 296, 329, 342, 446, 533
FaqI GGGAC 2 cut(s) 285, 323
FatI CATG 5 cut(s) 111, 219, 294, 327, 444
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 4 cut(s) 6, 88, 198, 370
Fsp4HI GCNGC 4 cut(s) 6, 88, 198, 370
FspBI CTAG 1 cut(s) 15
GlaI GCGC 1 cut(s) 279
GluI GCNGC 4 cut(s) 6, 88, 198, 370
HaeIII GGCC 3 cut(s) 133, 242, 299
HapII CCGG 3 cut(s) 163, 398, 425
HhaI GCGC 1 cut(s) 280
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 5 cut(s) 115, 223, 298, 331, 448
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HpaII CCGG 3 cut(s) 163, 398, 425
HphI GGTGA 2 cut(s) 85, 412
Hpy166II GTNNAC 3 cut(s) 217, 348, 493
Hpy188I TCNGA 1 cut(s) 393
Hpy188III TCNNGA 1 cut(s) 328
Hpy8I GTNNAC 3 cut(s) 217, 348, 493
Hpy99I CGWCG 2 cut(s) 122, 125
HpyAV CCTTC 1 cut(s) 35
HpyCH4IV ACGT 3 cut(s) 53, 120, 338
HpyCH4V TGCA 7 cut(s) 38, 47, 103, 115, 158, 471, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 84, 378, 468
HpySE526I ACGT 3 cut(s) 53, 120, 338
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 5 cut(s) 115, 223, 298, 331, 448
HspAI GCGC 1 cut(s) 278
LmnI GCTCC 2 cut(s) 27, 356
LpnPI CCDG 9 cut(s) 109, 165, 176, 261, 278, 336, 411, 426, 438
Lsp1109I GCAGC 2 cut(s) 99, 381
LweI GCATC 2 cut(s) 99, 411
MaeI CTAG 1 cut(s) 15
MaeII ACGT 3 cut(s) 53, 120, 338
MaeIII GTNAC 2 cut(s) 10, 523
MboII GAAGA 2 cut(s) 20, 422
MluCI AATT 1 cut(s) 507
MmeI TCCRAC 2 cut(s) 195, 371
MnlI CCTC 5 cut(s) 67, 310, 332, 335, 428
MroXI GAANNNNTTC 1 cut(s) 484
MseI TTAA 1 cut(s) 50
MslI CAYNNNNRTG 3 cut(s) 52, 98, 443
MspI CCGG 3 cut(s) 163, 398, 425
MspR9I CCNGG 1 cut(s) 398
Mva1269I GAATGC 1 cut(s) 105
MwoI GCNNNNNNNGC 4 cut(s) 44, 84, 378, 468
NciI CCSGG 1 cut(s) 398
NcoI CCATGG 1 cut(s) 294
NlaIII CATG 5 cut(s) 115, 223, 298, 331, 448
NlaIV GGNNCC 2 cut(s) 189, 395
NmuCI GTSAC 2 cut(s) 10, 523
NspI RCATGY 2 cut(s) 115, 448
PagI TCATGA 1 cut(s) 327
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 484
PkrI GCNGC 4 cut(s) 7, 89, 199, 371
PspN4I GGNNCC 2 cut(s) 189, 395
PspPI GGNCC 4 cut(s) 131, 187, 427, 493
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
RseI CAYNNNNRTG 3 cut(s) 52, 98, 443
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 4 cut(s) 6, 88, 198, 370
Sau96I GGNCC 4 cut(s) 131, 187, 427, 493
ScrFI CCNGG 1 cut(s) 398
SetI ASST 5 cut(s) 24, 56, 123, 341, 361
SfaNI GCATC 2 cut(s) 99, 411
SinI GGWCC 3 cut(s) 187, 427, 493
SmiMI CAYNNNNRTG 3 cut(s) 52, 98, 443
Sse9I AATT 1 cut(s) 507
SsiI CCGC 5 cut(s) 129, 144, 198, 261, 462
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 2 cut(s) 294, 430
TaiI ACGT 3 cut(s) 56, 123, 341
TasI AATT 1 cut(s) 507
TauI GCSGC 1 cut(s) 200
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 2 cut(s) 355, 445
TseFI GTSAC 2 cut(s) 10, 523
TseI GCWGC 3 cut(s) 5, 87, 369
Tsp45I GTSAC 2 cut(s) 10, 523
TspDTI ATGAA 1 cut(s) 515
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 2 cut(s) 355, 445
VpaK11BI GGWCC 3 cut(s) 187, 427, 493
XceI RCATGY 2 cut(s) 115, 448
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 1 cut(s) 15
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.