MD14G1242700.v1.1

Belongs to the group II decarboxylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0028329
Physical Location & Seq
Reverse (-)
32108140 .. 32110957
2818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1242700.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGGTGATCTCAACGACTTCCGCTGAGCGTCGTGGAGAGCAGGTGAACTGTACCTTTGCTTCCAGATATGTGCGTAACGTCCTCCCTAAGTTTCAGATGCCGGAGACGTCGATGCCAAAGGACTCGGCGTATCAGATAATAAACGACGAGCTGATGCTGGACGGGAATCCGCGGCTGAACTTGGCGTCGTTCGTGACGACGTGGATGGAGCCTGAATGCGATCGGCTGATGATGGCTTCCATGAACAAAAACTACGTCGACATGGATGAGTACCCTGTTACTACTGAACTCCAGAATCGTTGTGTGAATATTATCGCAAATCTGTTTAATGCTCCCATTGGAGACGGCGAAACTGCTGTTGGTGTGTCAACAGTAGGTTCTTCAGAGGCAATGATGCTGGCAGGCCTAGCTTTCAAAAGGAAGTGGCAGAACAAGAGAAAATTAGAGGGAAAGCCTTTTGATAAGCCCAACATGGTCACCGGAGCTAATGTGCAGGTTTGTTGGGAAAAATTCGCTAGGTATTTCGAAGTTGAACTGAAGGAGGTGAAGCTGTCGGAGGGATACTACGTGATGGACCCTGCGAAAGCAGTTGAGATGGTTGATGAGAACACTATCTGTGTTGCTGCTATTCTAGGCTCAACCCTAACAGGAGAGTTCGAAGACGTGAAGCTCCTTCATGATCTCCTTGTTGAAAAGAATAAGCAGACGGGCTGGGACACTCCCATTCATGTCGACGCTGCCAGCGGAGGCTTTATTGCTCCGTTTCTGTATCCCGAGCTCGAGTGGGATTTCCGCTTGCCGTTAGTCAAGAGCATCAATGTGAGTGGTCACAAGTATGGCCTTGTGTACGCCGGTGTTGGGTGGGTTGTGTGGAGGAGTAAGGAGGACTTGCCGGACGAGCTTATATTTCACATCAATTACCTTGGATCTGATCAGCCCACTTTCACCCTCAACTTTTCTAAAGGTACTGAATTATACATAATCTATCAATTTGCTATGTAG

Protein Analysis

334

Amino Acids

37.67

Weight (kDa)

4.95

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 34 - 330 4.9e-87 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028329)

Species Orthologous Gene IDs
malus_domestica MD14G1242700.v1.1
pyrus_communis pycom14g20390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 2 cut(s) 31, 484
AccI GTMKAC 2 cut(s) 258, 732
AccII CGCG 1 cut(s) 172
AciI CCGC 5 cut(s) 21, 170, 172, 744, 793
AclWI GGATC 1 cut(s) 934
AcsI RAATTY 1 cut(s) 509
AcuI CTGAAG 2 cut(s) 366, 557
AcyI GRCGYC 2 cut(s) 107, 185
AfaI GTAC 4 cut(s) 52, 272, 848, 967
AfiI CCNNNNNNNGG 1 cut(s) 858
AgsI TTSAA 3 cut(s) 415, 533, 692
AjiI CACGTC 2 cut(s) 201, 664
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 7 cut(s) 151, 410, 485, 550, 670, 778, 901
AluI AGCT 7 cut(s) 151, 410, 485, 550, 670, 778, 901
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 2 cut(s) 98, 336
AlwI GGATC 1 cut(s) 934
Ama87I CYCGRG 2 cut(s) 773, 779
AoxI GGCC 2 cut(s) 403, 838
ApeKI GCWGC 2 cut(s) 623, 737
ApoI RAATTY 1 cut(s) 509
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspS9I GGNCC 1 cut(s) 574
AsuHPI GGTGA 5 cut(s) 16, 55, 469, 556, 937
AsuII TTCGAA 2 cut(s) 525, 657
AvaI CYCGRG 2 cut(s) 773, 779
AvaII GGWCC 1 cut(s) 574
BanII GRGCYC 1 cut(s) 780
BarI GAAGNNNNNNTAC 2 cut(s) 43, 75
BbsI GAAGAC 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 2 cut(s) 610, 724
BccI CCATC 4 cut(s) 199, 226, 565, 589
BceAI ACGGC 2 cut(s) 361, 784
BciVI GTATCC 2 cut(s) 554, 780
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 2 cut(s) 98, 336
BfaI CTAG 3 cut(s) 407, 516, 632
BfuAI ACCTGC 2 cut(s) 31, 484
BfuI GTATCC 2 cut(s) 554, 780
BisI GCNGC 3 cut(s) 173, 624, 738
BlpI GCTNAGC 1 cut(s) 24
BlsI GCNGC 3 cut(s) 174, 625, 739
Bme18I GGWCC 1 cut(s) 574
BmeT110I CYCGRG 2 cut(s) 773, 779
BmgBI CACGTC 2 cut(s) 201, 664
BmgT120I GGNCC 1 cut(s) 574
BmiI GGNNCC 2 cut(s) 210, 576
BmsI GCATC 5 cut(s) 87, 102, 144, 384, 822
BpiI GAAGAC 1 cut(s) 666
BpmI CTGGAG 1 cut(s) 275
Bpu1102I GCTNAGC 1 cut(s) 24
Bpu14I TTCGAA 2 cut(s) 525, 657
BsaAI YACGTR 1 cut(s) 568
BsaHI GRCGYC 2 cut(s) 107, 185
BsaJI CCNNGG 2 cut(s) 170, 922
BsaWI WCCGGW 1 cut(s) 479
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 858
Bse118I RCCGGY 1 cut(s) 851
Bse3DI GCAATG 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 170, 922
BseGI GGATG 2 cut(s) 210, 271
BseLI CCNNNNNNNGG 1 cut(s) 858
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 889
BseXI GCAGC 2 cut(s) 610, 724
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 1 cut(s) 512
Bsh1236I CGCG 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 2 cut(s) 405, 840
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 780
BsiHKCI CYCGRG 2 cut(s) 773, 779
BsiSI CCGG 4 cut(s) 101, 480, 852, 893
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 1 cut(s) 858
BsmAI GTCTC 2 cut(s) 98, 336
BsmBI CGTCTC 2 cut(s) 98, 336
BsmFI GGGAC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 2 cut(s) 405, 840
BsoBI CYCGRG 2 cut(s) 773, 779
Bsp119I TTCGAA 2 cut(s) 525, 657
Bsp1286I GDGCHC 1 cut(s) 780
Bsp143I GATC 5 cut(s) 6, 220, 679, 926, 931
Bsp1720I GCTNAGC 1 cut(s) 24
BspACI CCGC 5 cut(s) 21, 170, 172, 744, 793
BspANI GGCC 2 cut(s) 405, 840
BspCNI CTCAG 1 cut(s) 16
BspFNI CGCG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 210, 576
BspMI ACCTGC 2 cut(s) 31, 484
BspPI GGATC 1 cut(s) 934
BspT104I TTCGAA 2 cut(s) 525, 657
BsrDI GCAATG 1 cut(s) 396
BsrFI RCCGGY 1 cut(s) 851
BssAI RCCGGY 1 cut(s) 851
BssECI CCNNGG 2 cut(s) 170, 922
BssMI GATC 5 cut(s) 6, 220, 679, 926, 931
BssNI GRCGYC 2 cut(s) 107, 185
BssT1I CCWWGG 1 cut(s) 922
Bst4CI ACNGT 2 cut(s) 50, 373
BstACI GRCGYC 2 cut(s) 107, 185
BstBAI YACGTR 1 cut(s) 568
BstBI TTCGAA 2 cut(s) 525, 657
BstC8I GCNNGC 4 cut(s) 399, 403, 742, 797
BstDEI CTNAG 2 cut(s) 24, 87
BstDSI CCRYGG 1 cut(s) 170
BstEII GGTNACC 1 cut(s) 475
BstF5I GGATG 2 cut(s) 210, 271
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 5 cut(s) 9, 223, 682, 929, 934
BstMAI GTCTC 2 cut(s) 98, 336
BstMBI GATC 5 cut(s) 6, 220, 679, 926, 931
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 407, 898
BstPI GGTNACC 1 cut(s) 475
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 610, 724
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 1 cut(s) 926
BstYI RGATCY 1 cut(s) 926
BsuI GTATCC 2 cut(s) 554, 780
BsuRI GGCC 2 cut(s) 405, 840
BtgI CCRYGG 1 cut(s) 170
BtrI CACGTC 2 cut(s) 201, 664
BtsCI GGATG 2 cut(s) 210, 271
BveI ACCTGC 2 cut(s) 31, 484
Cac8I GCNNGC 4 cut(s) 399, 403, 742, 797
CciI TCATGA 1 cut(s) 676
Cfr10I RCCGGY 1 cut(s) 851
Cfr13I GGNCC 1 cut(s) 574
Cfr42I CCGCGG 1 cut(s) 173
CseI GACGC 3 cut(s) 17, 174, 743
Csp6I GTAC 4 cut(s) 51, 271, 847, 966
CspCI CAANNNNNGTGG 2 cut(s) 805, 840
CviAII CATG 5 cut(s) 241, 262, 472, 677, 728
CviQI GTAC 4 cut(s) 51, 271, 847, 966
DdeI CTNAG 2 cut(s) 24, 87
DpnI GATC 5 cut(s) 8, 222, 681, 928, 933
DpnII GATC 5 cut(s) 6, 220, 679, 926, 931
Ecl136II GAGCTC 1 cut(s) 778
Eco130I CCWWGG 1 cut(s) 922
Eco147I AGGCCT 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 780
Eco47I GGWCC 1 cut(s) 574
Eco53kI GAGCTC 1 cut(s) 778
Eco57I CTGAAG 2 cut(s) 366, 557
Eco88I CYCGRG 2 cut(s) 773, 779
Eco91I GGTNACC 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 778
EcoO65I GGTNACC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 922
EcoT38I GRGCYC 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 922
Esp3I CGTCTC 2 cut(s) 98, 336
FaeI CATG 5 cut(s) 244, 265, 475, 680, 731
FalI AAGNNNNNCTT 2 cut(s) 872, 904
FaqI GGGAC 1 cut(s) 728
FatI CATG 5 cut(s) 240, 261, 471, 676, 727
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 2 cut(s) 258, 732
Fnu4HI GCNGC 3 cut(s) 173, 624, 738
FokI GGATG 2 cut(s) 217, 278
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 3 cut(s) 173, 624, 738
FspBI CTAG 3 cut(s) 407, 516, 632
GluI GCNGC 3 cut(s) 173, 624, 738
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 2 cut(s) 405, 840
HapII CCGG 4 cut(s) 101, 480, 852, 893
HgaI GACGC 3 cut(s) 17, 174, 743
Hin1I GRCGYC 2 cut(s) 107, 185
Hin1II CATG 5 cut(s) 244, 265, 475, 680, 731
HincII GTYRAC 3 cut(s) 259, 369, 733
HindII GTYRAC 3 cut(s) 259, 369, 733
HinfI GANTC 3 cut(s) 122, 166, 295
HpaII CCGG 4 cut(s) 101, 480, 852, 893
HphI GGTGA 5 cut(s) 16, 55, 469, 556, 937
Hpy166II GTNNAC 5 cut(s) 46, 259, 369, 733, 847
Hpy188I TCNGA 5 cut(s) 96, 135, 385, 556, 931
Hpy188III TCNNGA 6 cut(s) 63, 193, 292, 677, 773, 808
Hpy8I GTNNAC 5 cut(s) 46, 259, 369, 733, 847
Hpy99I CGWCG 7 cut(s) 33, 112, 149, 190, 202, 260, 737
HpyAV CCTTC 2 cut(s) 532, 683
HpyCH4III ACNGT 2 cut(s) 50, 373
HpyCH4IV ACGT 6 cut(s) 78, 107, 200, 255, 567, 663
HpyCH4V TGCA 1 cut(s) 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 898
HpyF3I CTNAG 2 cut(s) 24, 87
HpySE526I ACGT 6 cut(s) 78, 107, 200, 255, 567, 663
Hsp92I GRCGYC 2 cut(s) 107, 185
Hsp92II CATG 5 cut(s) 244, 265, 475, 680, 731
Ksp22I TGATCA 1 cut(s) 931
KspI CCGCGG 1 cut(s) 173
Kzo9I GATC 5 cut(s) 6, 220, 679, 926, 931
LmnI GCTCC 5 cut(s) 208, 337, 482, 675, 763
Lsp1109I GCAGC 2 cut(s) 610, 724
LweI GCATC 5 cut(s) 87, 102, 144, 384, 822
MaeI CTAG 3 cut(s) 407, 516, 632
MaeII ACGT 6 cut(s) 78, 107, 200, 255, 567, 663
MaeIII GTNAC 5 cut(s) 74, 193, 277, 475, 827
MalI GATC 5 cut(s) 8, 222, 681, 928, 933
MboI GATC 5 cut(s) 6, 220, 679, 926, 931
MboII GAAGA 2 cut(s) 372, 671
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 1 cut(s) 780
MluCI AATT 5 cut(s) 440, 509, 916, 971, 989
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 9 cut(s) 92, 379, 439, 535, 550, 740, 867, 877, 959
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 2 cut(s) 818, 834
MspA1I CMGCKG 3 cut(s) 23, 172, 744
MspI CCGG 4 cut(s) 101, 480, 852, 893
Mva1269I GAATGC 1 cut(s) 221
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 2 cut(s) 407, 898
NdeII GATC 5 cut(s) 6, 220, 679, 926, 931
NlaIII CATG 5 cut(s) 244, 265, 475, 680, 731
NlaIV GGNNCC 2 cut(s) 210, 576
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 3 cut(s) 193, 475, 827
NspV TTCGAA 2 cut(s) 525, 657
PaeR7I CTCGAG 1 cut(s) 779
PagI TCATGA 1 cut(s) 676
PaqCI CACCTGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 405
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 166, 295
PkrI GCNGC 3 cut(s) 174, 625, 739
Ple19I CGATCG 1 cut(s) 223
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Ppu21I YACGTR 1 cut(s) 568
Psp124BI GAGCTC 1 cut(s) 780
PspEI GGTNACC 1 cut(s) 475
PspFI CCCAGC 1 cut(s) 711
PspN4I GGNNCC 2 cut(s) 210, 576
PspPI GGNCC 1 cut(s) 574
PspXI VCTCGAGB 1 cut(s) 779
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PsuI RGATCY 1 cut(s) 926
PvuI CGATCG 1 cut(s) 223
RsaI GTAC 4 cut(s) 52, 272, 848, 967
RsaNI GTAC 4 cut(s) 51, 271, 847, 966
RseI CAYNNNNRTG 2 cut(s) 818, 834
SacI GAGCTC 1 cut(s) 780
SacII CCGCGG 1 cut(s) 173
SalI GTCGAC 2 cut(s) 257, 731
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 3 cut(s) 173, 624, 738
Sau3AI GATC 5 cut(s) 6, 220, 679, 926, 931
Sau96I GGNCC 1 cut(s) 574
SchI GAGTC 1 cut(s) 116
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 5 cut(s) 87, 102, 144, 384, 822
Sfr274I CTCGAG 1 cut(s) 779
Sfr303I CCGCGG 1 cut(s) 173
SfuI TTCGAA 2 cut(s) 525, 657
SgrAI CRCCGGYG 1 cut(s) 851
SgrBI CCGCGG 1 cut(s) 173
SinI GGWCC 1 cut(s) 574
SlaI CTCGAG 1 cut(s) 779
SmiMI CAYNNNNRTG 2 cut(s) 818, 834
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
Sse9I AATT 5 cut(s) 440, 509, 916, 971, 989
SseBI AGGCCT 1 cut(s) 405
SsiI CCGC 5 cut(s) 21, 170, 172, 744, 793
SspI AATATT 1 cut(s) 310
SspMI CTAG 3 cut(s) 407, 516, 632
SstI GAGCTC 1 cut(s) 780
StuI AGGCCT 1 cut(s) 405
StyI CCWWGG 1 cut(s) 922
TaaI ACNGT 2 cut(s) 50, 373
TaiI ACGT 6 cut(s) 81, 110, 203, 258, 570, 666
TaqI TCGA 6 cut(s) 110, 258, 525, 657, 732, 780
TasI AATT 5 cut(s) 440, 509, 916, 971, 989
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 2 cut(s) 166, 295
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseFI GTSAC 3 cut(s) 193, 475, 827
TseI GCWGC 2 cut(s) 623, 737
Tsp45I GTSAC 3 cut(s) 193, 475, 827
TspDTI ATGAA 3 cut(s) 257, 665, 716
TspGWI ACGGA 1 cut(s) 750
VpaK11BI GGWCC 1 cut(s) 574
XapI RAATTY 1 cut(s) 509
XhoI CTCGAG 1 cut(s) 779
XmiI GTMKAC 2 cut(s) 258, 732
XspI CTAG 3 cut(s) 407, 516, 632
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.