MD15G1008600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028432
Physical Location & Seq
Reverse (-)
452517 .. 454767
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1008600.v1.1.491

Sequence Viewer

Length: 525 bp
ATGTATGTAAAAAACCCTCGGAGTTTCCGAAAAATCGCTTCCAGTAATACTTCGTTGGCTGGGTCAGTAGGTGTTGGTAAGGTTTCTAAGATGGAACCCGTAGCTTTTGTTTCAGACAAGATACGAGGTTTCACGAAATCGACCCAGGAACTGTTCGGCAGCCTCGTCCATGGCCGCCCTCAGCCTTCAGACCCCCGACATCCGAATTCGTGGCCGGCCAAAGGCACAAGAGTGGGCATTGATCTTTCAGAATGCCCACCTTCTCTAGCAAAAGCTTCCTATACGGCAAATGGTAGCAACTGGTTGTCTGCAATTGCAATTCCAGTGGGAGCTCAGTGCAGGGATGTTGCGGTTATTACTAGTCCTCTCACCAGGGGAAGGGCCTTACTGATCTTTCATCATTTGGGCCACTGGCAGTGCAATTGGCTTAATGGTGAAAAAAATCAAATGTTGTTGTTTCCTTGGCTCAGTTTGTTTCCGTACTGGCAATGCAACCGAGTTCAGGTAGGGTTGGGCGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.04

Weight (kDa)

10.03

Isoelectric Point (pI)

23.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028432)

Species Orthologous Gene IDs
malus_domestica MD15G1008600.v1.1
pyrus_communis pycom15g00710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 175, 350
AcoI YGGCCR 3 cut(s) 172, 212, 216
AcsI RAATTY 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 3 cut(s) 221, 378, 502
AhlI ACTAGT 1 cut(s) 359
AjnI CCWGG 2 cut(s) 144, 371
AleI CACNNNNGTG 1 cut(s) 230
AluBI AGCT 3 cut(s) 104, 275, 332
AluI AGCT 3 cut(s) 104, 275, 332
Alw21I GWGCWC 1 cut(s) 334
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 5 cut(s) 172, 212, 216, 381, 406
ApeKI GCWGC 2 cut(s) 159, 518
ApoI RAATTY 1 cut(s) 205
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 2 cut(s) 381, 406
AsuHPI GGTGA 2 cut(s) 361, 446
BanII GRGCYC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 334
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 171, 505
BccI CCATC 1 cut(s) 85
BceAI ACGGC 1 cut(s) 300
BciT130I CCWGG 2 cut(s) 146, 373
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 2 cut(s) 266, 360
BfoI RGCGCY 1 cut(s) 519
BisI GCNGC 3 cut(s) 160, 175, 519
BlsI GCNGC 3 cut(s) 161, 176, 520
Bme1390I CCNGG 2 cut(s) 146, 373
BmgT120I GGNCC 2 cut(s) 381, 406
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 2 cut(s) 146, 373
Bpu10I CCTNAGC 1 cut(s) 180
BsaJI CCNNGG 5 cut(s) 17, 144, 169, 372, 461
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 378, 502
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 5 cut(s) 42, 305, 323, 416, 488
Bse3DI GCAATG 1 cut(s) 494
BseBI CCWGG 2 cut(s) 146, 373
BseDI CCNNGG 5 cut(s) 17, 144, 169, 372, 461
BseGI GGATG 2 cut(s) 199, 349
BseLI CCNNNNNNNGG 3 cut(s) 221, 378, 502
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 3 cut(s) 194, 347, 481
BseNI ACTGG 5 cut(s) 42, 305, 323, 416, 488
BseXI GCAGC 2 cut(s) 171, 505
BseYI CCCAGC 1 cut(s) 59
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 5 cut(s) 174, 214, 218, 383, 408
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 221, 378, 502
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 5 cut(s) 174, 214, 218, 383, 408
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 2 cut(s) 241, 390
Bsp19I CCATGG 1 cut(s) 169
BspACI CCGC 2 cut(s) 175, 350
BspANI GGCC 5 cut(s) 174, 214, 218, 383, 408
BspCNI CTCAG 3 cut(s) 193, 346, 480
BspLI GGNNCC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 5 cut(s) 42, 305, 323, 416, 488
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 5 cut(s) 17, 144, 169, 372, 461
BssMI GATC 2 cut(s) 241, 390
BssT1I CCWWGG 2 cut(s) 169, 461
Bst2UI CCWGG 2 cut(s) 146, 373
Bst4CI ACNGT 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 4 cut(s) 87, 180, 333, 467
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 2 cut(s) 199, 349
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 2 cut(s) 244, 393
BstMBI GATC 2 cut(s) 241, 390
BstNI CCWGG 2 cut(s) 146, 373
BstSCI CCNGG 2 cut(s) 144, 371
BstV1I GCAGC 2 cut(s) 171, 505
BsuRI GGCC 5 cut(s) 174, 214, 218, 383, 408
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 2 cut(s) 199, 349
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 4 cut(s) 330, 341, 409, 422
Cac8I GCNNGC 1 cut(s) 216
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 1 cut(s) 518
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 381, 406
Csp6I GTAC 1 cut(s) 481
CspCI CAANNNNNGTGG 2 cut(s) 306, 341
CviAII CATG 1 cut(s) 170
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 4 cut(s) 87, 180, 333, 467
DpnI GATC 2 cut(s) 243, 392
DpnII GATC 2 cut(s) 241, 390
EaeI YGGCCR 3 cut(s) 172, 212, 216
Ecl136II GAGCTC 1 cut(s) 332
Eco130I CCWWGG 2 cut(s) 169, 461
Eco24I GRGCYC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 144, 371
EcoT14I CCWWGG 2 cut(s) 169, 461
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 2 cut(s) 169, 461
FaeI CATG 1 cut(s) 173
FaiI YATR 3 cut(s) 6, 171, 282
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 3 cut(s) 160, 175, 519
FokI GGATG 2 cut(s) 186, 356
FriOI GRGCYC 1 cut(s) 334
FseI GGCCGGCC 1 cut(s) 218
Fsp4HI GCNGC 3 cut(s) 160, 175, 519
FspBI CTAG 2 cut(s) 266, 360
GlaI GCGC 1 cut(s) 517
GluI GCNGC 3 cut(s) 160, 175, 519
GsaI CCCAGC 1 cut(s) 63
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 5 cut(s) 174, 214, 218, 383, 408
HapII CCGG 1 cut(s) 215
HhaI GCGC 1 cut(s) 518
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HindIII AAGCTT 1 cut(s) 273
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 361, 446
Hpy188I TCNGA 6 cut(s) 21, 29, 115, 190, 204, 250
Hpy188III TCNNGA 1 cut(s) 133
HpyAV CCTTC 3 cut(s) 195, 270, 372
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4V TGCA 5 cut(s) 311, 317, 339, 420, 492
HpyF3I CTNAG 4 cut(s) 87, 180, 333, 467
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 1 cut(s) 516
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 2 cut(s) 241, 390
LmnI GCTCC 1 cut(s) 329
Lsp1109I GCAGC 2 cut(s) 171, 505
MaeI CTAG 2 cut(s) 266, 360
MalI GATC 2 cut(s) 243, 392
MboI GATC 2 cut(s) 241, 390
MfeI CAATTG 2 cut(s) 312, 421
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 4 cut(s) 205, 312, 318, 421
MnlI CCTC 5 cut(s) 27, 119, 173, 189, 375
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 230
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 2 cut(s) 146, 373
MunI CAATTG 2 cut(s) 312, 421
Mva1269I GAATGC 1 cut(s) 257
MvaI CCWGG 2 cut(s) 146, 373
NaeI GCCGGC 1 cut(s) 216
NcoI CCATGG 1 cut(s) 169
NdeII GATC 2 cut(s) 241, 390
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 1 cut(s) 96
OliI CACNNNNGTG 1 cut(s) 230
PcsI WCGNNNNNNNCGW 2 cut(s) 25, 162
PctI GAATGC 1 cut(s) 257
PdiI GCCGGC 1 cut(s) 216
PkrI GCNGC 3 cut(s) 161, 176, 520
Psp124BI GAGCTC 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 144, 371
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 2 cut(s) 144, 371
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 2 cut(s) 381, 406
PstNI CAGNNNCTG 1 cut(s) 151
RigI GGCCGGCC 1 cut(s) 218
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
RseI CAYNNNNRTG 1 cut(s) 230
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 3 cut(s) 160, 175, 519
Sau3AI GATC 2 cut(s) 241, 390
Sau96I GGNCC 2 cut(s) 381, 406
ScrFI CCNGG 2 cut(s) 146, 373
SduI GDGCHC 1 cut(s) 334
SetI ASST 8 cut(s) 73, 84, 106, 130, 262, 277, 334, 507
SmiMI CAYNNNNRTG 1 cut(s) 230
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 4 cut(s) 205, 312, 318, 421
SsiI CCGC 2 cut(s) 175, 350
SspMI CTAG 2 cut(s) 266, 360
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 2 cut(s) 144, 371
StyI CCWWGG 2 cut(s) 169, 461
TaaI ACNGT 1 cut(s) 153
TaqI TCGA 1 cut(s) 140
TasI AATT 4 cut(s) 205, 312, 318, 421
TauI GCSGC 1 cut(s) 177
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 4 cut(s) 330, 341, 416, 422
TseI GCWGC 2 cut(s) 159, 518
TspDTI ATGAA 1 cut(s) 386
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 4 cut(s) 330, 341, 416, 422
XapI RAATTY 1 cut(s) 205
XspI CTAG 2 cut(s) 266, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.