MD15G1115000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028427
Physical Location & Seq
Forward (+)
8106188 .. 8114440
8253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1115000.v1.1.491

Sequence Viewer

Length: 3666 bp
ATGAGCGGAAAACTCCACTGCCCATTTCTTGGGGTTTCACTACACAGCTCTTTAAATGGTAGAAACAATGGAAAATTCATTTGTTGGGACAGAGGGCGTACGGGAAAAAGGGCTGCTCGCAGGTGTGTATGTGAAAAGCAGAACTATTGGATTACTCAAGCGATTAAGGTTTCACATTTTTGGGGAAAAAATGTGGAGTTATTAAGGAGAACCTTCGAGCTAAAAAATGGGATGAAGGTGCAATGTGTTAAAGAGCCCTTTTCTCAAAGCAAGGCTCTGACGTGGTCTTTGTCTCATCTGTTGGAAGAGGGGTTGCTTTTAATTAGGTGCTCTGTATTTTTGGCCACCATATCCGGTGTATGCTTATTGGTATGGTATGGGCAATTAAAAGCCAAGGGTTTCATTGAAGCCAAGCTTTTGCCTTCTGTTTGTTCAGTACTCAGTGAATATATTCAGCGTGAAGTTGTTTTTGGTAAGGTTCGGAGGTTATCGCCTTTGAGCGTCACACTGGACTCTTGTTCAATTGGGCCTCATAATGAGGAATTTTCTTGTGGTGAGGTCCCATCTATGAAACTTCGGCTTCGTCCTTTTGCAAGTCTGAGGAGGGGAAGGATTGTGGTTGATGCAGTTTTGTTGCATCCGACGGTCTTGGTTGCACAGAAGAAGGATTATTCTTGGTTAGGGATACCCTCAAATGAAGGTGGTCTACAGAGGCACTTATCTAACGAAGAGGGAATTGATCATCGAACCAAAATCCGAAGGCTTGCTCGAGAAGCAGCAGCTGCTTGCTGGGAAAGAGAAAGAGATGAAGCGGCAAAAAAAGCTGCAGAGATGGGTTACATTGTTACTGACAAGGGTTCTAGTCCTGCAAATGTAAATGACTCGAAGGAAGGTGATAGCCATTCAGTAGATTTAACAAGTTCTGAGTCATTTCCGTGCATGGATGAAAAGATGTACTGGAGGGATCATTGCATGGACACAGGTGTTGATTATGAAATAAAGCATGCAGATTTGGAGAAATCGTTGGGTGTCAAAATTCCTGGGTTAGGGCTTAAATTTTGGTCCAGATTGATAAGGGGGCCTAAAAAGCACAAATTTAAAAGGAAGGGTTATGGGAGTGATATCTCTACTTCCGGTATTACTGCCAAAAGAAGAATTCTCGGGTACAGTGCAGCAAGGGCACTTGCATATTTTCAGGATCTATTTCATCAGAAGTCTGAGCCTTCACAGTCATCTGGAGGTTATGATGTGATGAACTTTGACACCTATTTGTTCAACAATGTGGCTGATACTAATGCTGACGCTTCTATCACAAGTACTGGTGAAAAAACTGTAAGTGACGATAACCACAATGGAAAACATTATGGAGATTCAGCAGACCATCCTCTTAAAGAAAATGAGAACGTCAACAGTCATTTAAGTAGTTCTAATTTCATAGAAGATCTACTTCCTATGACTTTAGATAGGTCTACTGGAGATGGGACATCCGGTAAAACTTTCCCATCCGCTGAAAATGTTGCAGGAGCTAATACCAAAAGTGGTAACGTAAAAGAGGATTTTGGAGTTGATGATGTAAATAAGCATACCGGTGATGAGATATCGGAGGGGCAAAGTGGTCAGATGTCGCAGAATTCAAATTTTGCTACATACGATCAAGTTCCTATCTGGCCCCTAAGCCTGAAATTAGGCTTGTCCTCCTTGTCTAGGTATGCAGCAGAGCCATTAGCCTTTCTCTCTGGACCCATTCAAAAGCTGATGTCTATGGGCCCCAGAGTTGAAGATATTGTTGCAGAACTTGTTGACGGGGTCGGTGTTGTGCAGTCTGAAGGCTTTGAGAAGATACTTCCAGTTACACTAGATTCTGTTCATTTCAAAGGAGGAACATTGATGCTGCTTGCATATGGCGATAGGGAACCTCGAGAGATGGAGAATGTCGATGGACATGTGAAGTTTCAAAATCTCTATGGTCGGGCTCACGTTCAAGTTAGTGGAAACTGTAAGATGTGGAGATCTGAAAACATATCTGAAGATGGTGGCTGGTTGTCTACGGATGTTTTTGTTGACATTGTTGAACAGAAATGGCATGCCAACTTAAAAGTTGCCAACTTGTTTGTTCCGCTTTTTGAAAGGATATTAGAAATTCCAATAATTTGGTCCAAAGGAAGAGCCACTGGTGAGGTTCACTTGTGCATGTCAAATGGAGAAACATTTCCAAATCTTCATGGACAGCTTGATGTGACAGGGTTGGCTTTTCAAACAATTGATGCGCCATCATCATTTTCTGATATTTCAGCAAGCTTATGTTTCCGTGGCCAACAAATATTCCTACACAATGCAAGTGGCTGGTTTGGTGATATTCCCTTGGAAGCTTCTGGAGATTTTGGCATTCATCCTGAGGAAGGAGAGTTTCATCTTATGTGTCAGCTGGCTGGTTCAGTGACTGCTGTGTTTAACTGTCAAGGTCCACTGGATGCACCTATATTTGTGGGAAGTGGAATGGTCTCTAGAAGGATATCTCATCCAGTTGCTGATTTTCCTCCGTCCTCTGCATGTGAAGCAGTTTTGGAAAGTAAGGAAGCTGGTGCAGTAGCAGCAATTGATCGTGTACCATTTTCCTGCTTATCGGCCAATTTCACTTATAACACTGAGAGCTGTGTTGTTGATTTGTATGGAATAAGAGCTAGCCTTGTAGATGGTGGTGAAATTCGAGGGGCAGGGAATGCTTGGATTTGCCCAGAGGGTGAGGTTGATGATACATCAATGGATGTGAATTTCTCTGGAAGTTTGTGTTTTGATAAAATTCTGCATCGATATATACCTGGGTACCTTCAACTAATGCCACTCAAGTTAGGGGCTTTAAATGGAGAGACAAAAATTTCTGGTTCGCTGTTGAGACCGAGGTGTGACATTAAATGGACAGCTCCAAAAGCTGAAGGGTCCTTTAGTGATGCTCGAGGAGATATCATAATCTCACATGATTCCATTACTGTTAATTCTTCATCTGCTGCTTTTGATTTGTCTTCGAAAGTTCAAACATCTTACACTGACGAAGTATGCTTGCACAGAAGTGATGGTTATGCAAAGAATGCAATGCCATTTGTTGTTGAGGGAATTGAATTGGATTTGCGTATGCATAATTTTGAGTTTTTTAACTGGGTATCTCCTTATCCTTTCGATTCTCCAAAACCTATGCATTTGAAAGCAACAGGCAAGATCAAGTTTCAAGGCAAGGTGTTAAAGCCTTGTAGTGTTAATAATGGACAAGATTTTGGTCTAGAGAGAAATAATTTACCTGTGGAGATGACAGATAAAGAAAAGACAGAGAGCCTTGTTGGAGAGGTTTCGATTTCAGGTCTTAGATTGAATCAGTTGATGTTGGCGCCTCAGTTGGCTGGATCATTGAGTTTGTCCCGTGAGTATGTCAAGTTGGATGCCACTGGTAGGCCAGATGAAAGTCTTGTAGTGGAGTTTGTTGGGCCCTTGAAGCCTAATAGTGAGGATAATTCTCAAAGCGGGAAGTTGGTATCTTTTTTACTTCAAAAGGGGCAACTAAAAGCAAACATCTGCTTCCAGCCATTTCATTCTGCTAACTTGGAGATACGCCAGTTACCACTGGATGAGTTGGAGTTGGCATCACTCCGTGGAACAATACAGAAGGCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TamB

Protein Analysis

1222

Amino Acids

134.37

Weight (kDa)

6.05

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028427)

Species Orthologous Gene IDs
malus_domestica MD15G1115000.v1.1
pyrus_communis pycom15g10370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2640
AarI CACCTGC 1 cut(s) 111
Acc36I ACCTGC 1 cut(s) 111
Acc65I GGTACC 1 cut(s) 2823
AccB1I GGYRCC 2 cut(s) 2823, 3379
AccB7I CCANNNNNTGG 1 cut(s) 29
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 3 cut(s) 706, 1469, 2045
AciI CCGC 5 cut(s) 6, 812, 1506, 2117, 3513
AclWI GGATC 3 cut(s) 972, 1206, 3403
AcoI YGGCCR 3 cut(s) 342, 2311, 2625
AcuI CTGAAG 3 cut(s) 1845, 2046, 2952
AcyI GRCGYC 1 cut(s) 3380
AdeI CACNNNGTG 1 cut(s) 3641
AfaI GTAC 7 cut(s) 100, 438, 956, 1166, 1318, 2607, 2825
AfiI CCNNNNNNNGG 5 cut(s) 29, 1046, 1704, 2399, 3441
AflIII ACRYGT 1 cut(s) 1942
AgeI ACCGGT 1 cut(s) 1586
AjiI CACGTC 1 cut(s) 282
AjnI CCWGG 2 cut(s) 1039, 2818
AjuI GAANNNNNNNTTGG 2 cut(s) 453, 485
AleI CACNNNNGTG 1 cut(s) 3066
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 4 cut(s) 297, 2506, 2861, 2887
AlwI GGATC 3 cut(s) 972, 1206, 3403
AlwNI CAGNNNCTG 3 cut(s) 782, 2441, 2528
Ama87I CYCGRG 4 cut(s) 768, 1160, 1917, 2952
AoxI GGCC 9 cut(s) 342, 527, 1079, 1667, 1765, 2311, 2625, 3443, 3476
ApaI GGGCCC 2 cut(s) 1769, 3480
ArsI GACNNNNNNTTYG 2 cut(s) 271, 303
AsiGI ACCGGT 1 cut(s) 1586
Asp700I GAANNNNTTC 2 cut(s) 450, 2208
Asp718I GGTACC 1 cut(s) 2823
AspLEI GCGC 2 cut(s) 2269, 3382
AsuHPI GGTGA 8 cut(s) 566, 905, 1334, 1601, 2186, 2363, 2711, 2753
AsuII TTCGAA 1 cut(s) 3023
AsuNHI GCTAGC 1 cut(s) 2681
AvaI CYCGRG 4 cut(s) 768, 1160, 1917, 2952
AvaII GGWCC 6 cut(s) 559, 1062, 1739, 2154, 2462, 2937
AxyI CCTNAGG 1 cut(s) 2394
BaeGI GKGCMC 3 cut(s) 1183, 1769, 3480
BaeI ACNNNNGTAYC 4 cut(s) 2807, 2840, 3590, 3623
BalI TGGCCA 2 cut(s) 344, 2313
BanI GGYRCC 2 cut(s) 2823, 3379
BanII GRGCYC 4 cut(s) 258, 1769, 1975, 3480
BarI GAAGNNNNNNTAC 2 cut(s) 690, 722
BbsI GAAGAC 1 cut(s) 3012
Bbv12I GWGCWC 1 cut(s) 332
BciT130I CCWGG 2 cut(s) 1041, 2820
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 4 cut(s) 297, 2506, 2861, 2887
BfaI CTAG 7 cut(s) 861, 1704, 1856, 2505, 2682, 3275, 3664
BfmI CTRYAG 2 cut(s) 707, 825
BfoI RGCGCY 1 cut(s) 3383
BfuAI ACCTGC 1 cut(s) 111
BfuI GTATCC 1 cut(s) 678
BglII AGATCT 2 cut(s) 1441, 2009
BmcAI AGTACT 2 cut(s) 438, 1318
Bme1390I CCNGG 2 cut(s) 1041, 2820
Bme18I GGWCC 6 cut(s) 559, 1062, 1739, 2154, 2462, 2937
BmeT110I CYCGRG 4 cut(s) 768, 1160, 1917, 2952
BmgBI CACGTC 1 cut(s) 282
BmrFI CCNGG 2 cut(s) 1041, 2820
BmrI ACTGGG 1 cut(s) 3163
BmsI GCATC 9 cut(s) 613, 646, 1878, 2254, 2461, 2815, 2938, 3421, 3641
BmtI GCTAGC 1 cut(s) 2685
BmuI ACTGGG 1 cut(s) 3163
BpiI GAAGAC 1 cut(s) 3012
BpmI CTGGAG 4 cut(s) 979, 1257, 1494, 2394
Bpu10I CCTNAGC 1 cut(s) 1673
Bpu14I TTCGAA 1 cut(s) 3023
BpuEI CTTGAG 2 cut(s) 141, 2828
Bsa29I ATCGAT 1 cut(s) 2809
BsaHI GRCGYC 1 cut(s) 3380
BsaI GGTCTC 2 cut(s) 2506, 2887
BsaJI CCNNGG 7 cut(s) 393, 1040, 2308, 2361, 2819, 2897, 3640
BsaWI WCCGGW 4 cut(s) 353, 1133, 1487, 1586
BsaXI ACNNNNNCTCC 2 cut(s) 1007, 1037
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 1046, 1704, 2399, 3441
Bse118I RCCGGY 1 cut(s) 1586
Bse21I CCTNAGG 1 cut(s) 2394
Bse3DI GCAATG 3 cut(s) 248, 967, 3096
BseBI CCWGG 2 cut(s) 1041, 2820
BseCI ATCGAT 1 cut(s) 2809
BseDI CCNNGG 7 cut(s) 393, 1040, 2308, 2361, 2819, 2897, 3640
BseLI CCNNNNNNNGG 5 cut(s) 29, 1046, 1704, 2399, 3441
BseMI GCAATG 3 cut(s) 248, 967, 3096
BseMII CTCAG 7 cut(s) 454, 590, 915, 1209, 2385, 2637, 3398
BseRI GAGGAG 2 cut(s) 616, 2970
BseSI GKGCMC 3 cut(s) 1183, 1769, 3480
BseYI CCCAGC 1 cut(s) 789
BsgI GTGCAG 3 cut(s) 1191, 1838, 2604
BshFI GGCC 9 cut(s) 344, 529, 1081, 1669, 1767, 2313, 2627, 3445, 3478
BshNI GGYRCC 2 cut(s) 2823, 3379
BshTI ACCGGT 1 cut(s) 1586
BshVI ATCGAT 1 cut(s) 2809
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 4 cut(s) 768, 1160, 1917, 2952
BsiSI CCGG 4 cut(s) 354, 1134, 1488, 1587
BsiWI CGTACG 1 cut(s) 98
BslFI GGGAC 4 cut(s) 101, 545, 1495, 3394
BslI CCNNNNNNNGG 5 cut(s) 29, 1046, 1704, 2399, 3441
BsmAI GTCTC 4 cut(s) 297, 2506, 2861, 2887
BsmFI GGGAC 4 cut(s) 101, 545, 1495, 3394
BsmI GAATGC 3 cut(s) 2385, 2725, 3091
BsnI GGCC 9 cut(s) 344, 529, 1081, 1669, 1767, 2313, 2627, 3445, 3478
Bso31I GGTCTC 2 cut(s) 2506, 2887
BsoBI CYCGRG 4 cut(s) 768, 1160, 1917, 2952
Bsp119I TTCGAA 1 cut(s) 3023
Bsp120I GGGCCC 2 cut(s) 1765, 3476
Bsp1286I GDGCHC 6 cut(s) 258, 332, 1183, 1769, 1975, 3480
Bsp143I GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
BspACI CCGC 5 cut(s) 6, 812, 1506, 2117, 3513
BspANI GGCC 9 cut(s) 344, 529, 1081, 1669, 1767, 2313, 2627, 3445, 3478
BspCNI CTCAG 7 cut(s) 453, 591, 916, 1210, 2386, 2638, 3397
BspDI ATCGAT 1 cut(s) 2809
BspMAI CTGCAG 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 111
BspOI GCTAGC 1 cut(s) 2685
BspPI GGATC 3 cut(s) 972, 1206, 3403
BspQI GCTCTTC 1 cut(s) 2158
BspT104I TTCGAA 1 cut(s) 3023
BspT107I GGYRCC 2 cut(s) 2823, 3379
BspTNI GGTCTC 2 cut(s) 2506, 2887
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 3 cut(s) 248, 967, 3096
BsrFI RCCGGY 1 cut(s) 1586
BssAI RCCGGY 1 cut(s) 1586
BssECI CCNNGG 7 cut(s) 393, 1040, 2308, 2361, 2819, 2897, 3640
BssMI GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
BssNI GRCGYC 1 cut(s) 3380
BssT1I CCWWGG 2 cut(s) 393, 2361
Bst2UI CCWGG 2 cut(s) 1041, 2820
Bst4CI ACNGT 8 cut(s) 646, 1169, 1230, 1333, 1412, 1997, 2456, 2989
Bst6I CTCTTC 3 cut(s) 300, 723, 2158
BstACI GRCGYC 1 cut(s) 3380
BstAPI GCANNNNNTGC 3 cut(s) 782, 2720, 3086
BstBI TTCGAA 1 cut(s) 3023
BstDEI CTNAG 9 cut(s) 440, 599, 924, 1218, 1673, 2394, 2646, 3356, 3384
BstDSI CCRYGG 2 cut(s) 2308, 3640
BstENI CCTNNNNNAGG 3 cut(s) 1044, 1702, 2397
BstH2I RGCGCY 1 cut(s) 3383
BstHHI GCGC 2 cut(s) 2269, 3382
BstKTI GATC 9 cut(s) 742, 967, 1201, 1444, 1654, 2012, 2602, 3216, 3398
BstMAI GTCTC 4 cut(s) 297, 2506, 2861, 2887
BstMBI GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
BstNI CCWGG 2 cut(s) 1041, 2820
BstNSI RCATGY 5 cut(s) 1007, 1946, 2087, 2194, 2553
BstSCI CCNGG 2 cut(s) 1039, 2818
BstSFI CTRYAG 2 cut(s) 707, 825
BstSLI GKGCMC 3 cut(s) 1183, 1769, 3480
BstV2I GAAGAC 1 cut(s) 3012
BstX2I RGATCY 3 cut(s) 1198, 1441, 2009
BstXI CCANNNNNNTGG 1 cut(s) 2151
BstYI RGATCY 3 cut(s) 1198, 1441, 2009
Bsu15I ATCGAT 1 cut(s) 2809
Bsu36I CCTNAGG 1 cut(s) 2394
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 9 cut(s) 344, 529, 1081, 1669, 1767, 2313, 2627, 3445, 3478
BsuTUI ATCGAT 1 cut(s) 2809
BtgI CCRYGG 2 cut(s) 2308, 3640
BtrI CACGTC 1 cut(s) 282
BtsI GCAGTG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 111
CaiI CAGNNNCTG 3 cut(s) 782, 2441, 2528
CfoI GCGC 2 cut(s) 2269, 3382
Cfr10I RCCGGY 1 cut(s) 1586
ClaI ATCGAT 1 cut(s) 2809
CseI GACGC 2 cut(s) 490, 1310
Csp6I GTAC 7 cut(s) 99, 437, 955, 1165, 1317, 2606, 2824
CspAI ACCGGT 1 cut(s) 1586
CspCI CAANNNNNGTGG 2 cut(s) 2320, 2355
CviAII CATG 9 cut(s) 940, 973, 1004, 1943, 2084, 2191, 2222, 2550, 2975
CviQI GTAC 7 cut(s) 99, 437, 955, 1165, 1317, 2606, 2824
DdeI CTNAG 9 cut(s) 440, 599, 924, 1218, 1673, 2394, 2646, 3356, 3384
DinI GGCGCC 1 cut(s) 3381
DpnI GATC 9 cut(s) 741, 966, 1200, 1443, 1653, 2011, 2601, 3215, 3397
DpnII GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
DraI TTTAAA 3 cut(s) 54, 1099, 2859
DraIII CACNNNGTG 1 cut(s) 3641
EaeI YGGCCR 3 cut(s) 342, 2311, 2625
Eam1104I CTCTTC 3 cut(s) 300, 723, 2158
EarI CTCTTC 3 cut(s) 300, 723, 2158
Eco130I CCWWGG 2 cut(s) 393, 2361
Eco24I GRGCYC 4 cut(s) 258, 1769, 1975, 3480
Eco31I GGTCTC 2 cut(s) 2506, 2887
Eco32I GATATC 4 cut(s) 1123, 1599, 2514, 2962
Eco47I GGWCC 6 cut(s) 559, 1062, 1739, 2154, 2462, 2937
Eco57I CTGAAG 3 cut(s) 1845, 2046, 2952
Eco81I CCTNAGG 1 cut(s) 2394
Eco88I CYCGRG 4 cut(s) 768, 1160, 1917, 2952
EcoNI CCTNNNNNAGG 3 cut(s) 1044, 1702, 2397
EcoO109I RGGNCCY 5 cut(s) 559, 1079, 1766, 2937, 3477
EcoRI GAATTC 2 cut(s) 1155, 1630
EcoRII CCWGG 2 cut(s) 1039, 2818
EcoRV GATATC 4 cut(s) 1123, 1599, 2514, 2962
EcoT14I CCWWGG 2 cut(s) 393, 2361
EcoT22I ATGCAT 2 cut(s) 3135, 3195
EcoT38I GRGCYC 4 cut(s) 258, 1769, 1975, 3480
EgeI GGCGCC 1 cut(s) 3381
EheI GGCGCC 1 cut(s) 3381
ErhI CCWWGG 2 cut(s) 393, 2361
FaeI CATG 9 cut(s) 943, 976, 1007, 1946, 2087, 2194, 2225, 2553, 2978
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 1431, 1463
FaqI GGGAC 4 cut(s) 101, 545, 1495, 3394
FatI CATG 9 cut(s) 939, 972, 1003, 1942, 2083, 2190, 2221, 2549, 2974
FauI CCCGC 1 cut(s) 3506
FauNDI CATATG 1 cut(s) 1900
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 3 cut(s) 706, 1469, 2045
FriOI GRGCYC 4 cut(s) 258, 1769, 1975, 3480
FspBI CTAG 7 cut(s) 861, 1704, 1856, 2505, 2682, 3275, 3664
GlaI GCGC 2 cut(s) 2268, 3381
GsaI CCCAGC 1 cut(s) 793
GsuI CTGGAG 4 cut(s) 979, 1257, 1494, 2394
HaeII RGCGCY 1 cut(s) 3383
HaeIII GGCC 9 cut(s) 344, 529, 1081, 1669, 1767, 2313, 2627, 3445, 3478
HapII CCGG 4 cut(s) 354, 1134, 1488, 1587
HgaI GACGC 2 cut(s) 490, 1310
HhaI GCGC 2 cut(s) 2269, 3382
Hin1I GRCGYC 1 cut(s) 3380
Hin1II CATG 9 cut(s) 943, 976, 1007, 1946, 2087, 2194, 2225, 2553, 2978
Hin6I GCGC 2 cut(s) 2267, 3380
HinP1I GCGC 2 cut(s) 2267, 3380
HincII GTYRAC 3 cut(s) 1408, 1801, 2062
HindII GTYRAC 3 cut(s) 1408, 1801, 2062
HindIII AAGCTT 3 cut(s) 413, 2296, 2367
HinfI GANTC 8 cut(s) 512, 881, 926, 1370, 1859, 2978, 3176, 3364
HpaII CCGG 4 cut(s) 354, 1134, 1488, 1587
HphI GGTGA 8 cut(s) 566, 905, 1334, 1601, 2186, 2363, 2711, 2753
Hpy166II GTNNAC 9 cut(s) 707, 1408, 1470, 1801, 2046, 2062, 2182, 2465, 2606
Hpy8I GTNNAC 9 cut(s) 707, 1408, 1470, 1801, 2046, 2062, 2182, 2465, 2606
Hpy99I CGWCG 1 cut(s) 646
HpyCH4III ACNGT 8 cut(s) 646, 1169, 1230, 1333, 1412, 1997, 2456, 2989
HpyCH4IV ACGT 4 cut(s) 281, 1404, 1545, 1977
HpyF3I CTNAG 9 cut(s) 440, 599, 924, 1218, 1673, 2394, 2646, 3356, 3384
HpySE526I ACGT 4 cut(s) 281, 1404, 1545, 1977
Hsp92I GRCGYC 1 cut(s) 3380
Hsp92II CATG 9 cut(s) 943, 976, 1007, 1946, 2087, 2194, 2225, 2553, 2978
HspAI GCGC 2 cut(s) 2267, 3380
KasI GGCGCC 1 cut(s) 3379
KpnI GGTACC 1 cut(s) 2827
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
LguI GCTCTTC 1 cut(s) 2158
LmnI GCTCC 2 cut(s) 1523, 2926
LweI GCATC 9 cut(s) 613, 646, 1878, 2254, 2461, 2815, 2938, 3421, 3641
MaeI CTAG 7 cut(s) 861, 1704, 1856, 2505, 2682, 3275, 3664
MaeII ACGT 4 cut(s) 281, 1404, 1545, 1977
MalI GATC 9 cut(s) 741, 966, 1200, 1443, 1653, 2011, 2601, 3215, 3397
MbiI CCGCTC 1 cut(s) 6
MboI GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
MfeI CAATTG 3 cut(s) 522, 2259, 2595
MflI RGATCY 3 cut(s) 1198, 1441, 2009
MhlI GDGCHC 6 cut(s) 258, 332, 1183, 1769, 1975, 3480
MlsI TGGCCA 2 cut(s) 344, 2313
MluNI TGGCCA 2 cut(s) 344, 2313
Mly113I GGCGCC 1 cut(s) 3380
MlyI GAGTC 3 cut(s) 506, 875, 935
MmeI TCCRAC 5 cut(s) 282, 665, 3313, 3408, 3603
Mox20I TGGCCA 2 cut(s) 344, 2313
Mph1103I ATGCAT 2 cut(s) 3135, 3195
MroXI GAANNNNTTC 2 cut(s) 450, 2208
MscI TGGCCA 2 cut(s) 344, 2313
MslI CAYNNNNRTG 3 cut(s) 934, 1587, 3066
Msp20I TGGCCA 2 cut(s) 344, 2313
MspA1I CMGCKG 3 cut(s) 782, 1508, 2425
MspI CCGG 4 cut(s) 354, 1134, 1488, 1587
MspR9I CCNGG 2 cut(s) 1041, 2820
MunI CAATTG 3 cut(s) 522, 2259, 2595
Mva1269I GAATGC 3 cut(s) 2385, 2725, 3091
MvaI CCWGG 2 cut(s) 1041, 2820
NarI GGCGCC 1 cut(s) 3380
NdeI CATATG 1 cut(s) 1900
NdeII GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
NheI GCTAGC 1 cut(s) 2681
NlaIII CATG 9 cut(s) 943, 976, 1007, 1946, 2087, 2194, 2225, 2553, 2978
NmuCI GTSAC 5 cut(s) 502, 1337, 2236, 2437, 2903
NsiI ATGCAT 2 cut(s) 3135, 3195
NspI RCATGY 5 cut(s) 1007, 1946, 2087, 2194, 2553
NspV TTCGAA 1 cut(s) 3023
OliI CACNNNNGTG 1 cut(s) 3066
PaeI GCATGC 2 cut(s) 1007, 2087
PaeR7I CTCGAG 3 cut(s) 768, 1917, 2952
PaqCI CACCTGC 1 cut(s) 111
PciI ACATGT 1 cut(s) 1942
PciSI GCTCTTC 1 cut(s) 2158
PctI GAATGC 3 cut(s) 2385, 2725, 3091
PdmI GAANNNNTTC 2 cut(s) 450, 2208
PfeI GAWTC 5 cut(s) 1370, 1859, 2978, 3176, 3364
Pfl23II CGTACG 1 cut(s) 98
PflFI GACNNNGTC 2 cut(s) 283, 1805
PflMI CCANNNNNTGG 1 cut(s) 29
PinAI ACCGGT 1 cut(s) 1586
PleI GAGTC 3 cut(s) 506, 875, 934
PluTI GGCGCC 1 cut(s) 3383
PpsI GAGTC 3 cut(s) 506, 875, 934
PpuMI RGGWCCY 2 cut(s) 559, 2937
PscI ACATGT 1 cut(s) 1942
PsiI TTATAA 1 cut(s) 2640
Psp5II RGGWCCY 2 cut(s) 559, 2937
Psp6I CCWGG 2 cut(s) 1039, 2818
PspFI CCCAGC 1 cut(s) 789
PspGI CCWGG 2 cut(s) 1039, 2818
PspLI CGTACG 1 cut(s) 98
PspOMI GGGCCC 2 cut(s) 1765, 3476
PspPPI RGGWCCY 2 cut(s) 559, 2937
PspXI VCTCGAGB 1 cut(s) 2952
PstI CTGCAG 1 cut(s) 829
PstNI CAGNNNCTG 3 cut(s) 782, 2441, 2528
PsuI RGATCY 3 cut(s) 1198, 1441, 2009
PsyI GACNNNGTC 2 cut(s) 283, 1805
PvuII CAGCTG 2 cut(s) 782, 2425
RsaI GTAC 7 cut(s) 100, 438, 956, 1166, 1318, 2607, 2825
RsaNI GTAC 7 cut(s) 99, 437, 955, 1165, 1317, 2606, 2824
RseI CAYNNNNRTG 3 cut(s) 934, 1587, 3066
SapI GCTCTTC 1 cut(s) 2158
Sau3AI GATC 9 cut(s) 739, 964, 1198, 1441, 1651, 2009, 2599, 3213, 3395
ScaI AGTACT 2 cut(s) 438, 1318
SchI GAGTC 3 cut(s) 506, 875, 935
ScrFI CCNGG 2 cut(s) 1041, 2820
SduI GDGCHC 6 cut(s) 258, 332, 1183, 1769, 1975, 3480
SfaNI GCATC 9 cut(s) 613, 646, 1878, 2254, 2461, 2815, 2938, 3421, 3641
SfcI CTRYAG 2 cut(s) 707, 825
SfoI GGCGCC 1 cut(s) 3381
Sfr274I CTCGAG 3 cut(s) 768, 1917, 2952
SfuI TTCGAA 1 cut(s) 3023
SinI GGWCC 6 cut(s) 559, 1062, 1739, 2154, 2462, 2937
SlaI CTCGAG 3 cut(s) 768, 1917, 2952
SmiMI CAYNNNNRTG 3 cut(s) 934, 1587, 3066
SmlI CTYRAG 5 cut(s) 156, 768, 1917, 2843, 2952
SmoI CTYRAG 5 cut(s) 156, 768, 1917, 2843, 2952
SphI GCATGC 2 cut(s) 1007, 2087
SsiI CCGC 5 cut(s) 6, 812, 1506, 2117, 3513
SspDI GGCGCC 1 cut(s) 3379
SspI AATATT 1 cut(s) 2322
SspMI CTAG 7 cut(s) 861, 1704, 1856, 2505, 2682, 3275, 3664
StyD4I CCNGG 2 cut(s) 1039, 2818
StyI CCWWGG 2 cut(s) 393, 2361
TaaI ACNGT 8 cut(s) 646, 1169, 1230, 1333, 1412, 1997, 2456, 2989
TaiI ACGT 4 cut(s) 284, 1407, 1548, 1980
TaqII GACCGA 1 cut(s) 2911
TatI WGTACW 3 cut(s) 436, 954, 1316
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 5 cut(s) 1370, 1859, 2978, 3176, 3364
TseFI GTSAC 5 cut(s) 502, 1337, 2236, 2437, 2903
Tsp45I GTSAC 5 cut(s) 502, 1337, 2236, 2437, 2903
TspGWI ACGGA 5 cut(s) 924, 2063, 2297, 2529, 3629
Tth111I GACNNNGTC 2 cut(s) 283, 1805
Van91I CCANNNNNTGG 1 cut(s) 29
VpaK11BI GGWCC 6 cut(s) 559, 1062, 1739, 2154, 2462, 2937
XagI CCTNNNNNAGG 3 cut(s) 1044, 1702, 2397
XbaI TCTAGA 2 cut(s) 2504, 3274
XceI RCATGY 5 cut(s) 1007, 1946, 2087, 2194, 2553
XhoI CTCGAG 3 cut(s) 768, 1917, 2952
XmiI GTMKAC 3 cut(s) 706, 1469, 2045
XmnI GAANNNNTTC 2 cut(s) 450, 2208
XspI CTAG 7 cut(s) 861, 1704, 1856, 2505, 2682, 3275, 3664
ZrmI AGTACT 2 cut(s) 438, 1318
Zsp2I ATGCAT 2 cut(s) 3135, 3195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.