MD15G1270800.v1.1

LYK5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0028423
Physical Location & Seq
Reverse (-)
23500607 .. 23501389
783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1270800.v1.1.491

Sequence Viewer

Length: 783 bp
ATGAACCTTGACGAGCATTGTCGAATTCGGAGTTCAATTTACAAAGGCAATATATATGGGAAGGATTTGGCCGTGAAGCAAACCAAGGGAGATATCAAGGAGGAGCTGAATATCCTCCAAAAAGTGAATCATTTGAATCTGGTGAGCTTGTTAGGCACTTCGTGCGAAACTGATGGGAGTCAGTGGTTCTTGGTTTACGATTATGCTGAGAACGGTTCACTTGAAAAGGGGTTGCACCACAACTCTGCAGCCTCTTCATCATCAAGCTCTGCGTTGCCTTGCCTCAGTTGGAGCCAAAGAATACACATAGCTTTAGATGTTGCGAGTGGTCTGCACTGCATGCATGATCACACGCAACCGAACATTGTTCACTGGGACGTCAGGTCGAAGAACATCCTTCTGGACTCCAAATTCAAGGCCAAGCTAGCAAATTTCTCAATGGCTAGAACTGTTGCAGACCCTACAATGGCGAAAGTGGATGTCTTCGCATTTGGGATCCTTCTGTTCGAGTTGCTCTCAGGAAAGAAGGCCTTGACAACAAAAGATAATGGAGTAAATGTAATGTTGTGCAAGGAAATAAGGGAGGGTTTGAGGCTTGAAGAGGAGAGAGTGGATACGATTATGAAGTTGATGGATCTGAAATTGGATAATCGCTATTCTATTGATGGTGCTTTGAGTTTGGCAGCCTTGGCATGGATATGCACTCAAGAAAAGGCTTTGGCAAGGCCGAAAATGGACAGAAGTCGTCTTTCACCTCTCGGTACTTGCTCAGTCATCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005775 GO:0005886 GO:0006464 GO:0006468 GO:0006486 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009605 GO:0009607 GO:0009617 GO:0009877 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0032101 GO:0032103 GO:0034645 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043413 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070013 GO:0070085 GO:0071704 GO:0071944 GO:0080134 GO:0140096 GO:1900150 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1902288 GO:1902290
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
LysM Pkinase Pkinase_Tyr

Protein Analysis

261

Amino Acids

28.95

Weight (kDa)

8.12

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 25 - 154 6.5e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 26 - 152 2.4e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028423)

Species Orthologous Gene IDs
malus_domestica MD15G1270800.v1.1
prunus_persica Prupe.7G147400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 381
AclWI GGATC 3 cut(s) 490, 503, 642
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 3 cut(s) 24, 410, 430
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 162
AfaI GTAC 1 cut(s) 763
AfiI CCNNNNNNNGG 2 cut(s) 466, 693
AgsI TTSAA 5 cut(s) 36, 136, 224, 415, 599
AhdI GACNNNNNGTC 1 cut(s) 382
AluBI AGCT 5 cut(s) 106, 147, 267, 311, 424
AluI AGCT 5 cut(s) 106, 147, 267, 311, 424
AlwI GGATC 3 cut(s) 490, 503, 642
AoxI GGCC 4 cut(s) 69, 417, 528, 725
ApeKI GCWGC 2 cut(s) 248, 683
ApoI RAATTY 3 cut(s) 24, 410, 430
AsuHPI GGTGA 2 cut(s) 154, 744
AsuNHI GCTAGC 1 cut(s) 424
BamHI GGATCC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 475
BbvI GCAGC 2 cut(s) 260, 695
BccI CCATC 3 cut(s) 167, 625, 659
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 1 cut(s) 346
BfaI CTAG 2 cut(s) 425, 444
BfmI CTRYAG 1 cut(s) 246
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 2 cut(s) 249, 684
BlsI GCNGC 2 cut(s) 250, 685
BmeRI GACNNNNNGTC 1 cut(s) 382
BmiI GGNNCC 2 cut(s) 293, 497
BmrI ACTGGG 1 cut(s) 382
BmtI GCTAGC 1 cut(s) 428
BmuI ACTGGG 1 cut(s) 382
BoxI GACNNNNGTC 1 cut(s) 741
BpiI GAAGAC 1 cut(s) 475
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpuEI CTTGAG 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 84, 687
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 2 cut(s) 466, 693
Bse1I ACTGG 1 cut(s) 377
BseDI CCNNGG 2 cut(s) 84, 687
BseGI GGATG 3 cut(s) 393, 484, 774
BseLI CCNNNNNNNGG 2 cut(s) 466, 693
BseMII CTCAG 4 cut(s) 198, 298, 531, 783
BseNI ACTGG 1 cut(s) 377
BseRI GAGGAG 2 cut(s) 116, 617
BseXI GCAGC 2 cut(s) 260, 695
BsgI GTGCAG 1 cut(s) 317
BshFI GGCC 4 cut(s) 71, 419, 530, 727
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 466, 693
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 4 cut(s) 71, 419, 530, 727
Bsp143I GATC 3 cut(s) 346, 495, 634
BspANI GGCC 4 cut(s) 71, 419, 530, 727
BspCNI CTCAG 4 cut(s) 199, 297, 530, 782
BspLI GGNNCC 2 cut(s) 293, 497
BspMAI CTGCAG 1 cut(s) 250
BspOI GCTAGC 1 cut(s) 428
BspPI GGATC 3 cut(s) 490, 503, 642
BsrI ACTGG 1 cut(s) 377
BssECI CCNNGG 2 cut(s) 84, 687
BssMI GATC 3 cut(s) 346, 495, 634
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 2 cut(s) 84, 687
Bst4CI ACNGT 2 cut(s) 215, 451
Bst6I CTCTTC 2 cut(s) 259, 594
BstACI GRCGYC 1 cut(s) 378
BstAPI GCANNNNNTGC 2 cut(s) 162, 340
BstC8I GCNNGC 2 cut(s) 341, 426
BstDEI CTNAG 4 cut(s) 207, 284, 517, 769
BstF5I GGATG 3 cut(s) 393, 484, 774
BstKTI GATC 3 cut(s) 349, 498, 637
BstMBI GATC 3 cut(s) 346, 495, 634
BstMWI GCNNNNNNNGC 5 cut(s) 153, 162, 340, 425, 689
BstNSI RCATGY 1 cut(s) 343
BstPAI GACNNNNGTC 1 cut(s) 741
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 2 cut(s) 260, 695
BstV2I GAAGAC 1 cut(s) 475
BstX2I RGATCY 2 cut(s) 495, 634
BstYI RGATCY 2 cut(s) 495, 634
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 4 cut(s) 71, 419, 530, 727
BtsCI GGATG 3 cut(s) 393, 484, 774
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 3 cut(s) 188, 334, 370
Cac8I GCNNGC 2 cut(s) 341, 426
Csp6I GTAC 1 cut(s) 762
CviAII CATG 3 cut(s) 340, 344, 693
CviQI GTAC 1 cut(s) 762
DdeI CTNAG 4 cut(s) 207, 284, 517, 769
DpnI GATC 3 cut(s) 348, 497, 636
DpnII GATC 3 cut(s) 346, 495, 634
DraIII CACNNNGTG 1 cut(s) 162
DriI GACNNNNNGTC 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 259, 594
Eam1105I GACNNNNNGTC 1 cut(s) 382
EarI CTCTTC 2 cut(s) 259, 594
Eco130I CCWWGG 2 cut(s) 84, 687
Eco147I AGGCCT 1 cut(s) 530
Eco32I GATATC 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 24
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 2 cut(s) 84, 687
EcoT22I ATGCAT 1 cut(s) 345
ErhI CCWWGG 2 cut(s) 84, 687
FaeI CATG 3 cut(s) 343, 347, 696
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 1 cut(s) 389
FatI CATG 3 cut(s) 339, 343, 692
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 2 cut(s) 249, 684
FokI GGATG 3 cut(s) 380, 491, 761
Fsp4HI GCNGC 2 cut(s) 249, 684
FspBI CTAG 2 cut(s) 425, 444
GluI GCNGC 2 cut(s) 249, 684
HaeIII GGCC 4 cut(s) 71, 419, 530, 727
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 3 cut(s) 343, 347, 696
HinfI GANTC 4 cut(s) 127, 136, 178, 404
HphI GGTGA 2 cut(s) 154, 744
Hpy166II GTNNAC 3 cut(s) 196, 218, 370
Hpy188I TCNGA 2 cut(s) 30, 639
Hpy188III TCNNGA 3 cut(s) 401, 519, 707
Hpy8I GTNNAC 3 cut(s) 196, 218, 370
HpyAV CCTTC 4 cut(s) 55, 407, 509, 520
HpyCH4III ACNGT 2 cut(s) 215, 451
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 8 cut(s) 235, 248, 334, 339, 343, 455, 570, 702
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 162, 340, 425, 689
HpyF3I CTNAG 4 cut(s) 207, 284, 517, 769
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 3 cut(s) 343, 347, 696
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 3 cut(s) 346, 495, 634
LmnI GCTCC 2 cut(s) 103, 291
LpnPI CCDG 5 cut(s) 125, 358, 367, 386, 504
Lsp1109I GCAGC 2 cut(s) 260, 695
MaeI CTAG 2 cut(s) 425, 444
MaeII ACGT 1 cut(s) 378
MalI GATC 3 cut(s) 348, 497, 636
MboI GATC 3 cut(s) 346, 495, 634
MboII GAAGA 4 cut(s) 246, 400, 475, 611
MflI RGATCY 2 cut(s) 495, 634
MluCI AATT 5 cut(s) 24, 36, 410, 430, 641
MlyI GAGTC 2 cut(s) 187, 398
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 8 cut(s) 94, 125, 262, 293, 577, 585, 595, 765
Mph1103I ATGCAT 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 697
MwoI GCNNNNNNNGC 5 cut(s) 153, 162, 340, 425, 689
NdeII GATC 3 cut(s) 346, 495, 634
NheI GCTAGC 1 cut(s) 424
NlaIII CATG 3 cut(s) 343, 347, 696
NlaIV GGNNCC 2 cut(s) 293, 497
NsiI ATGCAT 1 cut(s) 345
NspI RCATGY 1 cut(s) 343
PaeI GCATGC 1 cut(s) 343
PceI AGGCCT 1 cut(s) 530
PfeI GAWTC 2 cut(s) 127, 136
PkrI GCNGC 2 cut(s) 250, 685
PleI GAGTC 2 cut(s) 186, 398
PpsI GAGTC 2 cut(s) 186, 398
PshAI GACNNNNGTC 1 cut(s) 741
PspN4I GGNNCC 2 cut(s) 293, 497
PstI CTGCAG 1 cut(s) 250
PsuI RGATCY 2 cut(s) 495, 634
RsaI GTAC 1 cut(s) 763
RsaNI GTAC 1 cut(s) 762
RseI CAYNNNNRTG 1 cut(s) 697
SatI GCNGC 2 cut(s) 249, 684
Sau3AI GATC 3 cut(s) 346, 495, 634
SchI GAGTC 2 cut(s) 187, 398
SetI ASST 9 cut(s) 9, 108, 149, 269, 313, 381, 386, 426, 757
SfcI CTRYAG 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
SphI GCATGC 1 cut(s) 343
Sse9I AATT 5 cut(s) 24, 36, 410, 430, 641
SseBI AGGCCT 1 cut(s) 530
SspMI CTAG 2 cut(s) 425, 444
StuI AGGCCT 1 cut(s) 530
StyI CCWWGG 2 cut(s) 84, 687
TaaI ACNGT 2 cut(s) 215, 451
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 22, 386, 507
TasI AATT 5 cut(s) 24, 36, 410, 430, 641
TfiI GAWTC 2 cut(s) 127, 136
TscAI CASTG 3 cut(s) 188, 341, 377
TseI GCWGC 2 cut(s) 248, 683
TspDTI ATGAA 3 cut(s) 17, 246, 638
TspRI CASTG 3 cut(s) 188, 341, 377
XapI RAATTY 3 cut(s) 24, 410, 430
XceI RCATGY 1 cut(s) 343
XspI CTAG 2 cut(s) 425, 444
ZraI GACGTC 1 cut(s) 379
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.