MD16G1035300.v1.1

chromatin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0021165
Physical Location & Seq
Forward (+)
2524328 .. 2528503
4176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1035300.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGGGAGCTAAGGTTGCTGCTGCTGATCCTTCAAGTGCGATTGAGTGTGGTTGGTTACCTGGAGATGTTTTTGATGCCTTACCTTGCAAATATGTCAATGGAGCCATCCTTTGTGAGATCAATCTTGGAATTATGTGGAGCTGGAAAAGGCGAAAGCTGAGTAATGCATCAGACACCGACATAGATTTCAACAACTCTCATCATGTAGCTGACATTACCGTTAATTGTGCTACTCAGAGTTGCAGCTCTCTAGCTGGTTTATCGTATCAAGAAAGAGGCATTACATTTTCGGAGCACAATGTTGTACGAGAGAACTCATTATCCACAAGAAAACAACCTTCTTCAAGAGAATTGGGGAACTCACTTTCCAATCTGTTGACATCACAGCCTACATCTAAAGCTGCATATAGTTGTAGCCAGGCTTCTTTACCGATGGTTGCAGTGTCTGATTTTGCCGCTTTACCCAAACCAAGTCTTAACTACTCAAATAATGGTTTGGGAATGCCTGGTTCATCGGCTCTTGATAAGAGAGTCAGATGTGAAACACAAGCAGTTCAAGCACCAATGCTAAAGAAACCCAAGCCAGAGCCCGTGGACTTATCTTATCAGCAGCTTACTGGAAGCCAAGCTGAAACCAGTCTTCTACCTTATCTACAGTGGAAGAAAAAATTGTTGCATCAACCCAGTGAGGGTGGAAAAGTTATACATGAAAGAATCCAGGAGAAAGAGCGTCCCTTGGCGATAATAAATAATGGTCAGACAAGAAGTTTTCAAGGAATCTCAGAGCTGCAGGCTGCTTTCGACACTTCTAATGTTAAGTTAGAGCCTGAAAAAGATAGTTTCCCCAGTTCAAATTTTAGGAAGACCAAAAATGAGCACCGTGTGATGGACAGGATACCTGTTCAGTCTTATCTCCAGCAATCACAAAAGCAACAATCATCATCTCAATTAAGAGATAATGTATCTACTAATATGAGGATGCACAGAAGTGTCTCTCAATTGAGGACGGGTTCAGATATATTCCAGGTTGCAGCTCCTGAGAATTGTGCGTCTGCAAGTTCTCCAAACATAACCTTTCTGCCATCAGAGGTAGTTGTTCCTCCAAAGCAGAAAACAGATCCTCGTCCTAAACGCTCCAGAAAAAAGACTGATGATTCTAGTGCTAGCATGGGCAATACCAATGCTGCTGATGCAAACCATGTTTCCATGAATCTTACTTCATCTCGGCCATCAAGACCTAAAGCTGGATCTTTCCCTGAGAGCTTTTTGAAGATTCAAGCAGTGACTGAGAGGTATGGACTACATAACAGGGAGTACAAATTGGACCCATTTTTGCGAAGGAAACCCTTTTCTTCCGTAACTCCATTGGTTTCTTACCAACTCTTATCTTCAGGAGATAACAAAAAGTGGGAGAATACTTCCGCAGATAAAATTTCTCTCAAAGAATGCTTTCTGGACAGGGGTATCGGTATTTATGAAACTCGGACATTGACTTTTCTACGTCAATCTCACATCCATCAAGAAAATGAGACTCCCATCGTTGATAGAGAGGCTCAGGTCAGACTGATCATATCAGAGAAGCTGAATGAGGGAATGATGAAAGCAAGTGTTGCTTATGGAACCGAAAAAGAGATGTTTTCAATTGATTATCCTCTTCTACCGGCTTCTACTAGCACTCATTCTGCATACCTCTTGGCTACTCAGTTCACCACACTGGTATATGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

577

Amino Acids

63.89

Weight (kDa)

9.05

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHL PF20474 431 - 573 2.6e-29 PHL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021165)

Species Orthologous Gene IDs
malus_domestica MD16G1035300.v1.1
prunus_persica Prupe.1G319400_v2.0.a1 Prupe.1G319400_v2.0.a1 Prupe.1G319400_v2.0.a1 Prupe.1G319400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 456, 1422
AclWI GGATC 3 cut(s) 20, 1112, 1255
AcoI YGGCCR 1 cut(s) 1226
AcsI RAATTY 2 cut(s) 853, 1431
AcuI CTGAAG 1 cut(s) 1374
AdeI CACNNNGTG 1 cut(s) 883
AfaI GTAC 2 cut(s) 306, 1316
AfiI CCNNNNNNNGG 3 cut(s) 689, 886, 1244
AgsI TTSAA 9 cut(s) 33, 190, 345, 557, 773, 852, 1270, 1277, 1641
AjnI CCWGG 5 cut(s) 58, 417, 505, 717, 1023
AleI CACNNNNGTG 1 cut(s) 987
AloI GAACNNNNNNTCC 4 cut(s) 305, 337, 350, 382
Alw21I GWGCWC 2 cut(s) 297, 879
Alw26I GTCTC 2 cut(s) 997, 1523
AlwI GGATC 3 cut(s) 20, 1112, 1255
AlwNI CAGNNNCTG 3 cut(s) 446, 1037, 1286
AoxI GGCC 1 cut(s) 1226
ApeKI GCWGC 9 cut(s) 17, 20, 243, 401, 610, 787, 794, 1031, 1184
ApoI RAATTY 2 cut(s) 853, 1431
Asp700I GAANNNNTTC 1 cut(s) 1449
AspS9I GGNCC 1 cut(s) 1324
AsuHPI GGTGA 1 cut(s) 1699
AsuNHI GCTAGC 1 cut(s) 1163
AvaII GGWCC 1 cut(s) 1324
BaeI ACNNNNGTAYC 2 cut(s) 1447, 1480
BanII GRGCYC 1 cut(s) 591
BarI GAAGNNNNNNTAC 2 cut(s) 406, 438
BbsI GAAGAC 2 cut(s) 632, 869
Bbv12I GWGCWC 2 cut(s) 297, 879
BbvI GCAGC 9 cut(s) 4, 7, 255, 388, 622, 774, 781, 1043, 1171
BccI CCATC 7 cut(s) 113, 427, 880, 1090, 1237, 1524, 1544
BciT130I CCWGG 5 cut(s) 60, 419, 507, 719, 1025
BciVI GTATCC 1 cut(s) 888
BclI TGATCA 1 cut(s) 1566
BcoDI GTCTC 2 cut(s) 997, 1523
BfaI CTAG 4 cut(s) 251, 1158, 1164, 1671
BfmI CTRYAG 2 cut(s) 653, 788
BfuI GTATCC 1 cut(s) 888
Bme1390I CCNGG 5 cut(s) 60, 419, 507, 719, 1025
Bme18I GGWCC 1 cut(s) 1324
BmgT120I GGNCC 1 cut(s) 1324
BmiI GGNNCC 3 cut(s) 103, 1326, 1621
BmrFI CCNGG 5 cut(s) 60, 419, 507, 719, 1025
BmrI ACTGGG 2 cut(s) 678, 840
BmsI GCATC 5 cut(s) 64, 176, 685, 969, 1180
BmtI GCTAGC 1 cut(s) 1167
BmuI ACTGGG 2 cut(s) 678, 840
BpiI GAAGAC 2 cut(s) 632, 869
BpmI CTGGAG 3 cut(s) 81, 899, 1120
Bpu10I CCTNAGC 2 cut(s) 9, 1554
BsaJI CCNNGG 2 cut(s) 591, 735
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 689, 886, 1244
Bse118I RCCGGY 1 cut(s) 1660
Bse1I ACTGG 5 cut(s) 622, 636, 684, 846, 1719
BseBI CCWGG 5 cut(s) 60, 419, 507, 719, 1025
BseDI CCNNGG 2 cut(s) 591, 735
BseGI GGATG 3 cut(s) 105, 984, 1512
BseLI CCNNNNNNNGG 3 cut(s) 689, 886, 1244
BseMII CTCAG 8 cut(s) 149, 248, 795, 1029, 1248, 1278, 1568, 1715
BseNI ACTGG 5 cut(s) 622, 636, 684, 846, 1719
BseXI GCAGC 9 cut(s) 4, 7, 255, 388, 622, 774, 781, 1043, 1171
BshFI GGCC 1 cut(s) 1228
BsiHKAI GWGCWC 2 cut(s) 297, 879
BsiSI CCGG 1 cut(s) 1661
BslFI GGGAC 1 cut(s) 717
BslI CCNNNNNNNGG 3 cut(s) 689, 886, 1244
BsmAI GTCTC 2 cut(s) 997, 1523
BsmFI GGGAC 1 cut(s) 717
BsmI GAATGC 2 cut(s) 507, 1451
BsnI GGCC 1 cut(s) 1228
Bsp1286I GDGCHC 3 cut(s) 297, 591, 879
Bsp143I GATC 5 cut(s) 25, 117, 1117, 1247, 1566
BspACI CCGC 2 cut(s) 456, 1422
BspANI GGCC 1 cut(s) 1228
BspCNI CTCAG 8 cut(s) 150, 247, 794, 1030, 1249, 1279, 1567, 1714
BspLI GGNNCC 3 cut(s) 103, 1326, 1621
BspMAI CTGCAG 1 cut(s) 792
BspOI GCTAGC 1 cut(s) 1167
BspPI GGATC 3 cut(s) 20, 1112, 1255
BsrFI RCCGGY 1 cut(s) 1660
BsrI ACTGG 5 cut(s) 622, 636, 684, 846, 1719
BssAI RCCGGY 1 cut(s) 1660
BssECI CCNNGG 2 cut(s) 591, 735
BssMI GATC 5 cut(s) 25, 117, 1117, 1247, 1566
BssT1I CCWWGG 1 cut(s) 735
Bst2UI CCWGG 5 cut(s) 60, 419, 507, 719, 1025
Bst4CI ACNGT 3 cut(s) 220, 657, 881
Bst6I CTCTTC 1 cut(s) 1659
BstAPI GCANNNNNTGC 1 cut(s) 1610
BstC8I GCNNGC 2 cut(s) 792, 1165
BstDEI CTNAG 9 cut(s) 9, 158, 234, 781, 1038, 1257, 1287, 1554, 1701
BstDSI CCRYGG 1 cut(s) 591
BstEII GGTNACC 1 cut(s) 54
BstF5I GGATG 3 cut(s) 105, 984, 1512
BstKTI GATC 5 cut(s) 28, 120, 1120, 1250, 1569
BstMAI GTCTC 2 cut(s) 997, 1523
BstMBI GATC 5 cut(s) 25, 117, 1117, 1247, 1566
BstMWI GCNNNNNNNGC 4 cut(s) 14, 557, 1190, 1610
BstNI CCWGG 5 cut(s) 60, 419, 507, 719, 1025
BstPI GGTNACC 1 cut(s) 54
BstSCI CCNGG 5 cut(s) 58, 417, 505, 717, 1023
BstSFI CTRYAG 2 cut(s) 653, 788
BstV1I GCAGC 9 cut(s) 4, 7, 255, 388, 622, 774, 781, 1043, 1171
BstV2I GAAGAC 2 cut(s) 632, 869
BstX2I RGATCY 2 cut(s) 1117, 1247
BstYI RGATCY 2 cut(s) 1117, 1247
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 1 cut(s) 1228
BtgI CCRYGG 1 cut(s) 591
BtsCI GGATG 3 cut(s) 105, 984, 1512
BtsI GCAGTG 2 cut(s) 447, 1287
BtsIMutI CAGTG 5 cut(s) 447, 662, 691, 1287, 1712
Cac8I GCNNGC 2 cut(s) 792, 1165
CaiI CAGNNNCTG 3 cut(s) 446, 1037, 1286
Cfr10I RCCGGY 1 cut(s) 1660
Cfr13I GGNCC 1 cut(s) 1324
CseI GACGC 2 cut(s) 719, 1038
Csp6I GTAC 2 cut(s) 305, 1315
CviAII CATG 6 cut(s) 203, 707, 1168, 1199, 1207, 1728
CviQI GTAC 2 cut(s) 305, 1315
DdeI CTNAG 9 cut(s) 9, 158, 234, 781, 1038, 1257, 1287, 1554, 1701
DpnI GATC 5 cut(s) 27, 119, 1119, 1249, 1568
DpnII GATC 5 cut(s) 25, 117, 1117, 1247, 1566
DraIII CACNNNGTG 1 cut(s) 883
EaeI YGGCCR 1 cut(s) 1226
Eam1104I CTCTTC 1 cut(s) 1659
EarI CTCTTC 1 cut(s) 1659
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 591
Eco47I GGWCC 1 cut(s) 1324
Eco57I CTGAAG 1 cut(s) 1374
Eco91I GGTNACC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 54
EcoRII CCWGG 5 cut(s) 58, 417, 505, 717, 1023
EcoT14I CCWWGG 1 cut(s) 735
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 591
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 6 cut(s) 206, 710, 1171, 1202, 1210, 1731
FalI AAGNNNNNCTT 2 cut(s) 1597, 1629
FaqI GGGAC 1 cut(s) 717
FatI CATG 6 cut(s) 202, 706, 1167, 1198, 1206, 1727
FbaI TGATCA 1 cut(s) 1566
FokI GGATG 3 cut(s) 92, 991, 1499
FriOI GRGCYC 1 cut(s) 591
FspBI CTAG 4 cut(s) 251, 1158, 1164, 1671
GsuI CTGGAG 3 cut(s) 81, 899, 1120
HaeIII GGCC 1 cut(s) 1228
HapII CCGG 1 cut(s) 1661
HgaI GACGC 2 cut(s) 719, 1038
Hin1II CATG 6 cut(s) 206, 710, 1171, 1202, 1210, 1731
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 7 cut(s) 531, 714, 777, 1154, 1210, 1273, 1531
HpaII CCGG 1 cut(s) 1661
HphI GGTGA 1 cut(s) 1699
Hpy166II GTNNAC 3 cut(s) 378, 595, 1707
Hpy188III TCNNGA 9 cut(s) 269, 345, 521, 1037, 1137, 1233, 1392, 1454, 1520
Hpy8I GTNNAC 3 cut(s) 378, 595, 1707
HpyAV CCTTC 3 cut(s) 39, 348, 1332
HpyCH4III ACNGT 3 cut(s) 220, 657, 881
HpyCH4IV ACGT 1 cut(s) 1501
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 557, 1190, 1610
HpyF3I CTNAG 9 cut(s) 9, 158, 234, 781, 1038, 1257, 1287, 1554, 1701
HpySE526I ACGT 1 cut(s) 1501
Hsp92II CATG 6 cut(s) 206, 710, 1171, 1202, 1210, 1731
Ksp22I TGATCA 1 cut(s) 1566
Kzo9I GATC 5 cut(s) 25, 117, 1117, 1247, 1566
LmnI GCTCC 6 cut(s) 5, 101, 138, 292, 1039, 1139
Lsp1109I GCAGC 9 cut(s) 4, 7, 255, 388, 622, 774, 781, 1043, 1171
LweI GCATC 5 cut(s) 64, 176, 685, 969, 1180
MaeI CTAG 4 cut(s) 251, 1158, 1164, 1671
MaeII ACGT 1 cut(s) 1501
MaeIII GTNAC 3 cut(s) 54, 1282, 1357
MalI GATC 5 cut(s) 27, 119, 1119, 1249, 1568
MboI GATC 5 cut(s) 25, 117, 1117, 1247, 1566
MboII GAAGA 8 cut(s) 333, 632, 673, 874, 1282, 1344, 1380, 1646
MfeI CAATTG 2 cut(s) 998, 1641
MflI RGATCY 2 cut(s) 1117, 1247
MhlI GDGCHC 3 cut(s) 297, 591, 879
MlyI GAGTC 2 cut(s) 540, 1525
Mph1103I ATGCAT 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 1449
MseI TTAA 4 cut(s) 222, 477, 816, 950
MslI CAYNNNNRTG 1 cut(s) 987
MspI CCGG 1 cut(s) 1661
MspR9I CCNGG 5 cut(s) 60, 419, 507, 719, 1025
MunI CAATTG 2 cut(s) 998, 1641
Mva1269I GAATGC 2 cut(s) 507, 1451
MvaI CCWGG 5 cut(s) 60, 419, 507, 719, 1025
MwoI GCNNNNNNNGC 4 cut(s) 14, 557, 1190, 1610
NdeII GATC 5 cut(s) 25, 117, 1117, 1247, 1566
NheI GCTAGC 1 cut(s) 1163
NlaIII CATG 6 cut(s) 206, 710, 1171, 1202, 1210, 1731
NlaIV GGNNCC 3 cut(s) 103, 1326, 1621
NmeAIII GCCGAG 1 cut(s) 1204
NmuCI GTSAC 1 cut(s) 1282
NsiI ATGCAT 1 cut(s) 169
OliI CACNNNNGTG 1 cut(s) 987
PctI GAATGC 2 cut(s) 507, 1451
PdmI GAANNNNTTC 1 cut(s) 1449
PfeI GAWTC 5 cut(s) 714, 777, 1154, 1210, 1273
PfoI TCCNGGA 1 cut(s) 717
PleI GAGTC 2 cut(s) 539, 1525
PpsI GAGTC 2 cut(s) 539, 1525
Psp6I CCWGG 5 cut(s) 58, 417, 505, 717, 1023
PspEI GGTNACC 1 cut(s) 54
PspGI CCWGG 5 cut(s) 58, 417, 505, 717, 1023
PspN4I GGNNCC 3 cut(s) 103, 1326, 1621
PspPI GGNCC 1 cut(s) 1324
PstI CTGCAG 1 cut(s) 792
PstNI CAGNNNCTG 3 cut(s) 446, 1037, 1286
PsuI RGATCY 2 cut(s) 1117, 1247
RsaI GTAC 2 cut(s) 306, 1316
RsaNI GTAC 2 cut(s) 305, 1315
RseI CAYNNNNRTG 1 cut(s) 987
SaqAI TTAA 4 cut(s) 222, 477, 816, 950
Sau3AI GATC 5 cut(s) 25, 117, 1117, 1247, 1566
Sau96I GGNCC 1 cut(s) 1324
SchI GAGTC 2 cut(s) 540, 1525
ScrFI CCNGG 5 cut(s) 60, 419, 507, 719, 1025
SduI GDGCHC 3 cut(s) 297, 591, 879
SfaNI GCATC 5 cut(s) 64, 176, 685, 969, 1180
SfcI CTRYAG 2 cut(s) 653, 788
SinI GGWCC 1 cut(s) 1324
SmiMI CAYNNNNRTG 1 cut(s) 987
SsiI CCGC 2 cut(s) 456, 1422
SspMI CTAG 4 cut(s) 251, 1158, 1164, 1671
StyD4I CCNGG 5 cut(s) 58, 417, 505, 717, 1023
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 3 cut(s) 220, 657, 881
TaiI ACGT 1 cut(s) 1504
TaqI TCGA 1 cut(s) 801
TatI WGTACW 1 cut(s) 1314
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 5 cut(s) 714, 777, 1154, 1210, 1273
Tru1I TTAA 4 cut(s) 222, 477, 816, 950
Tru9I TTAA 4 cut(s) 222, 477, 816, 950
TscAI CASTG 5 cut(s) 447, 662, 691, 1287, 1719
TseFI GTSAC 1 cut(s) 1282
TseI GCWGC 9 cut(s) 17, 20, 243, 401, 610, 787, 794, 1031, 1184
Tsp45I GTSAC 1 cut(s) 1282
TspDTI ATGAA 6 cut(s) 501, 723, 1209, 1223, 1491, 1613
TspGWI ACGGA 1 cut(s) 1345
TspRI CASTG 5 cut(s) 447, 662, 691, 1287, 1719
VpaK11BI GGWCC 1 cut(s) 1324
XapI RAATTY 2 cut(s) 853, 1431
XmnI GAANNNNTTC 1 cut(s) 1449
XspI CTAG 4 cut(s) 251, 1158, 1164, 1671
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.