MD16G1155800.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0028534
Physical Location & Seq
Forward (+)
12394030 .. 12394692
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1155800.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGAGTCTTCTGCAGTCAAGTTTGTCCCGAACATACATTCAGGAAGCCACAGTGACACTGGATCAAGGGATTCCATGGATGACGAACACATTCGGATCGATGATCAATCTGCCCATGTGGGGCCTCGCTTCAAGTGTCCCTTTCCAAGTGCATTTTATGCGGTTTTTGACGGTCATGGAGGGCCTGAGGCAGCTGCTTATATCAAAAGGAATGCCATGAGACTATTCTTTGAAGATGCTGATTTACCGCAGAGAATGGATATGGATGCTGTTTTCTTCAGAGAGTTGGAGAATTCCCACAGGAAAGCATTTCTACTGGCAGACCATGCCTTGGCTGATGAACAAAGTGTGAGAAGTTCGTGTGGAACAACAGCTTTGACTGCTCTTATACTTGGAAGGCATTTAATGGTTGCAAATGCTGGTGACTGTCTAGCGGTTCTTTGCAGGAAAGGAATAGCAGTTGACATGTCTCAAGATCATAGACCTAGTTACTTGCCGGAACGTATGCGAGTTGAGCAGTTGGGTGGTTACATTGATGATGGATATCTTAACGGTTCTATCTCAGTCACTCGAGTTCTTGGAGATTGGGGCTTAAAATTACCATTGGGTTCCTCATTGCCTCTCATTGCCGAGCCAGATGTTCAGCAGGTTAACAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

221

Amino Acids

24.22

Weight (kDa)

5.53

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 13 - 218 2.4e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028534)

Species Orthologous Gene IDs
malus_domestica MD16G1155800.v1.1
pyrus_communis pycom13g12680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 637
AccB7I CCANNNNNTGG 1 cut(s) 331
AciI CCGC 3 cut(s) 161, 248, 434
AclWI GGATC 2 cut(s) 70, 104
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 262
AfiI CCNNNNNNNGG 2 cut(s) 120, 331
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 2 cut(s) 133, 233
AluBI AGCT 2 cut(s) 194, 374
AluI AGCT 2 cut(s) 194, 374
Alw26I GTCTC 2 cut(s) 214, 474
AlwI GGATC 2 cut(s) 70, 104
Ama87I CYCGRG 1 cut(s) 570
AoxI GGCC 2 cut(s) 122, 182
ApeKI GCWGC 2 cut(s) 191, 194
ApoI RAATTY 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 90
AspS9I GGNCC 2 cut(s) 122, 182
AsuHPI GGTGA 1 cut(s) 434
AvaI CYCGRG 1 cut(s) 570
AxyI CCTNAGG 1 cut(s) 186
BbvI GCAGC 2 cut(s) 181, 203
BccI CCATC 1 cut(s) 533
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 214, 474
BfaI CTAG 2 cut(s) 431, 486
BfmI CTRYAG 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 637
BisI GCNGC 2 cut(s) 192, 195
BlsI GCNGC 2 cut(s) 193, 196
BmeT110I CYCGRG 1 cut(s) 570
BmgT120I GGNCC 2 cut(s) 122, 182
BmiI GGNNCC 2 cut(s) 123, 610
BmsI GCATC 2 cut(s) 226, 256
BpuEI CTTGAG 1 cut(s) 456
Bsa29I ATCGAT 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 2 cut(s) 75, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 331
Bse1I ACTGG 2 cut(s) 64, 321
Bse21I CCTNAGG 1 cut(s) 186
Bse3DI GCAATG 2 cut(s) 614, 624
Bse8I GATNNNNATC 1 cut(s) 543
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 75, 330
BseGI GGATG 2 cut(s) 85, 271
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 2 cut(s) 120, 331
BseMI GCAATG 2 cut(s) 614, 624
BseMII CTCAG 2 cut(s) 177, 576
BseNI ACTGG 2 cut(s) 64, 321
BseXI GCAGC 2 cut(s) 181, 203
BshFI GGCC 2 cut(s) 124, 184
BshVI ATCGAT 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 570
BsiSI CCGG 1 cut(s) 497
BslFI GGGAC 2 cut(s) 11, 123
BslI CCNNNNNNNGG 2 cut(s) 120, 331
BsmAI GTCTC 2 cut(s) 214, 474
BsmFI GGGAC 2 cut(s) 11, 123
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 2 cut(s) 124, 184
BsoBI CYCGRG 1 cut(s) 570
Bsp143I GATC 4 cut(s) 62, 96, 103, 475
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 3 cut(s) 161, 248, 434
BspANI GGCC 2 cut(s) 124, 184
BspCNI CTCAG 2 cut(s) 178, 575
BspDI ATCGAT 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 123, 610
BspMAI CTGCAG 1 cut(s) 16
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 2 cut(s) 70, 104
BsrDI GCAATG 2 cut(s) 614, 624
BsrI ACTGG 2 cut(s) 64, 321
BssECI CCNNGG 2 cut(s) 75, 330
BssMI GATC 4 cut(s) 62, 96, 103, 475
BssT1I CCWWGG 2 cut(s) 75, 330
Bst4CI ACNGT 4 cut(s) 53, 173, 428, 554
BstAPI GCANNNNNTGC 2 cut(s) 158, 326
BstDEI CTNAG 2 cut(s) 186, 562
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 85, 271
BstKTI GATC 4 cut(s) 65, 99, 106, 478
BstMAI GTCTC 2 cut(s) 214, 474
BstMBI GATC 4 cut(s) 62, 96, 103, 475
BstMWI GCNNNNNNNGC 4 cut(s) 158, 326, 380, 514
BstNSI RCATGY 1 cut(s) 469
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 181, 203
Bsu15I ATCGAT 1 cut(s) 99
Bsu36I CCTNAGG 1 cut(s) 186
BsuRI GGCC 2 cut(s) 124, 184
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 2 cut(s) 85, 271
BtsIMutI CAGTG 2 cut(s) 57, 58
BveI ACCTGC 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 122, 182
ClaI ATCGAT 1 cut(s) 99
CviAII CATG 6 cut(s) 76, 116, 176, 217, 326, 466
CviJI RGCY 8 cut(s) 48, 124, 184, 194, 335, 374, 591, 634
CviKI_1 RGCY 8 cut(s) 48, 124, 184, 194, 335, 374, 591, 634
DdeI CTNAG 2 cut(s) 186, 562
DpnI GATC 4 cut(s) 64, 98, 105, 477
DpnII GATC 4 cut(s) 62, 96, 103, 475
Eco130I CCWWGG 2 cut(s) 75, 330
Eco32I GATATC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 570
EcoO109I RGGNCCY 2 cut(s) 122, 182
EcoRI GAATTC 1 cut(s) 292
EcoRV GATATC 1 cut(s) 545
EcoT14I CCWWGG 2 cut(s) 75, 330
ErhI CCWWGG 2 cut(s) 75, 330
FaeI CATG 6 cut(s) 79, 119, 179, 220, 329, 469
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FaqI GGGAC 2 cut(s) 11, 123
FatI CATG 6 cut(s) 75, 115, 175, 216, 325, 465
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 2 cut(s) 192, 195
FokI GGATG 2 cut(s) 92, 278
Fsp4HI GCNGC 2 cut(s) 192, 195
FspBI CTAG 2 cut(s) 431, 486
GluI GCNGC 2 cut(s) 192, 195
HaeIII GGCC 2 cut(s) 124, 184
HapII CCGG 1 cut(s) 497
Hin1II CATG 6 cut(s) 79, 119, 179, 220, 329, 469
HincII GTYRAC 2 cut(s) 463, 652
HindII GTYRAC 2 cut(s) 463, 652
HinfI GANTC 2 cut(s) 5, 71
HpaI GTTAAC 1 cut(s) 652
HpaII CCGG 1 cut(s) 497
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 463, 652
Hpy188I TCNGA 2 cut(s) 96, 281
Hpy188III TCNNGA 3 cut(s) 28, 42, 473
Hpy8I GTNNAC 2 cut(s) 463, 652
HpyAV CCTTC 1 cut(s) 390
HpyCH4III ACNGT 4 cut(s) 53, 173, 428, 554
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 4 cut(s) 14, 152, 413, 444
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 326, 380, 514
HpyF3I CTNAG 2 cut(s) 186, 562
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 6 cut(s) 79, 119, 179, 220, 329, 469
Ksp22I TGATCA 1 cut(s) 103
KspAI GTTAAC 1 cut(s) 652
Kzo9I GATC 4 cut(s) 62, 96, 103, 475
Lsp1109I GCAGC 2 cut(s) 181, 203
LweI GCATC 2 cut(s) 226, 256
MaeI CTAG 2 cut(s) 431, 486
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 5 cut(s) 53, 422, 488, 527, 565
MalI GATC 4 cut(s) 64, 98, 105, 477
MboI GATC 4 cut(s) 62, 96, 103, 475
MboII GAAGA 2 cut(s) 245, 268
MluCI AATT 2 cut(s) 292, 596
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 5 cut(s) 135, 173, 181, 622, 630
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 4 cut(s) 404, 549, 593, 651
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 1 cut(s) 497
Mva1269I GAATGC 1 cut(s) 217
MwoI GCNNNNNNNGC 4 cut(s) 158, 326, 380, 514
NcoI CCATGG 1 cut(s) 75
NdeII GATC 4 cut(s) 62, 96, 103, 475
NlaIII CATG 6 cut(s) 79, 119, 179, 220, 329, 469
NlaIV GGNNCC 2 cut(s) 123, 610
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 3 cut(s) 53, 422, 565
NspI RCATGY 1 cut(s) 469
PaeR7I CTCGAG 1 cut(s) 570
PciI ACATGT 1 cut(s) 465
PctI GAATGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 90
PfeI GAWTC 1 cut(s) 71
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 2 cut(s) 193, 196
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PscI ACATGT 1 cut(s) 465
PspN4I GGNNCC 2 cut(s) 123, 610
PspPI GGNCC 2 cut(s) 122, 182
PspXI VCTCGAGB 1 cut(s) 570
PstI CTGCAG 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 194
SaqAI TTAA 4 cut(s) 404, 549, 593, 651
SatI GCNGC 2 cut(s) 192, 195
Sau3AI GATC 4 cut(s) 62, 96, 103, 475
Sau96I GGNCC 2 cut(s) 122, 182
SchI GAGTC 1 cut(s) 14
SetI ASST 5 cut(s) 196, 376, 487, 505, 651
SfaNI GCATC 2 cut(s) 226, 256
SfcI CTRYAG 1 cut(s) 12
Sfr274I CTCGAG 1 cut(s) 570
SlaI CTCGAG 1 cut(s) 570
SmlI CTYRAG 2 cut(s) 471, 570
SmoI CTYRAG 2 cut(s) 471, 570
Sse9I AATT 2 cut(s) 292, 596
SsiI CCGC 3 cut(s) 161, 248, 434
SspMI CTAG 2 cut(s) 431, 486
StyI CCWWGG 2 cut(s) 75, 330
TaaI ACNGT 4 cut(s) 53, 173, 428, 554
TaiI ACGT 1 cut(s) 505
TaqI TCGA 2 cut(s) 99, 571
TasI AATT 2 cut(s) 292, 596
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 4 cut(s) 404, 549, 593, 651
Tru9I TTAA 4 cut(s) 404, 549, 593, 651
TscAI CASTG 2 cut(s) 58, 64
TseFI GTSAC 3 cut(s) 53, 422, 565
TseI GCWGC 2 cut(s) 191, 194
Tsp45I GTSAC 3 cut(s) 53, 422, 565
TspDTI ATGAA 1 cut(s) 354
TspRI CASTG 2 cut(s) 58, 64
Van91I CCANNNNNTGG 1 cut(s) 331
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 469
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XhoI CTCGAG 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 2 cut(s) 431, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.