Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0021164

Physical Location & Seq

Genomic Coordinates Forward (+)

28620716 .. 28623842

Gene Structure 3127 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1252900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 543 bp

ATGAATGATGTTTTTGGTAACTATGTGATTCAGAAGGCACTTGAGGTTATTGAACTTGAACAGAAAGTGCAGCTTGTACATGAGCTGGATGGGCATATTATGAGATGTATTCGTGATCAAAATGGAAACCATGTCATACAAAAGTGCATTGAGAGCATTCCTACAGAAAAAAATTGGGTTTATAATCTCTGCTTTCCGTGGTCAAGTTCCAACACTTTCAATCCATCCTTATGGCTGTCGTGTTATACAGTTCTGGAGCATTGTACAGATAAGCTGCAATGCCAGTTTATAGTTGACGAGATCTTGGAGTTTGTTGCTGGTCTTGCCCAGGACCAGTATGGCAACTATGTCACACAGGTAATGATGAAGGACCAATATGCAAATTATGTGGTGCAGAAGACTCTTGAAAATTGTACTGATAGTCAACGGGTGTTATTAATTAATCGTACAAGGGGCCATACAAACACATTGAAGAAGAACACTTACAGGAAGCACATCGTAGCTCGGTTTGAGCAGCTATTTGGTGAAGAAAATTTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632 NABP PUF

Protein Analysis

181

Amino Acids

21.07

Weight (kDa)

5.85

Isoelectric Point (pI)

28.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	3 - 29	3.1e-06	Pumilio-family RNA binding repeat
PUF	PF00806	121 - 148	2.8e-08	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0021164)

Species	Orthologous Gene IDs
malus_domestica	MD16G1252900.v1.1
pyrus_communis	pycom07g16550
rosa_multiflora	Rmu_sc0037811.1_g000001 Rmu_sc0039367.1_g000001 Rmu_sc0042890.1_g000001

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	183
AcsI	RAATTY	1 cut(s)	532
AfaI	GTAC	4 cut(s)	78, 265, 415, 448
AgsI	TTSAA	5 cut(s)	53, 59, 220, 407, 472
AjnI	CCWGG	1 cut(s)	327
AluBI	AGCT	5 cut(s)	73, 85, 274, 503, 517
AluI	AGCT	5 cut(s)	73, 85, 274, 503, 517
AoxI	GGCC	1 cut(s)	454
ApeKI	GCWGC	3 cut(s)	70, 274, 514
ApoI	RAATTY	1 cut(s)	532
AseI	ATTAAT	2 cut(s)	437, 441
AspS9I	GGNCC	3 cut(s)	331, 370, 454
AsuHPI	GGTGA	1 cut(s)	536
AvaII	GGWCC	2 cut(s)	331, 370
BarI	GAAGNNNNNNTAC	2 cut(s)	467, 499
BbsI	GAAGAC	1 cut(s)	404
BbvI	GCAGC	3 cut(s)	82, 261, 526
BccI	CCATC	2 cut(s)	83, 232
BciT130I	CCWGG	1 cut(s)	329
BclI	TGATCA	1 cut(s)	115
BfmI	CTRYAG	1 cut(s)	162
BglII	AGATCT	1 cut(s)	300
BisI	GCNGC	3 cut(s)	71, 275, 515
BlsI	GCNGC	3 cut(s)	72, 276, 516
Bme1390I	CCNGG	1 cut(s)	329
Bme18I	GGWCC	2 cut(s)	331, 370
BmgT120I	GGNCC	3 cut(s)	331, 370, 454
BmiI	GGNNCC	1 cut(s)	455
BmrFI	CCNGG	1 cut(s)	329
BpiI	GAAGAC	1 cut(s)	404
BpmI	CTGGAG	1 cut(s)	275
BpuEI	CTTGAG	1 cut(s)	62
BsaJI	CCNNGG	2 cut(s)	197, 327
Bse1I	ACTGG	2 cut(s)	283, 334
Bse3DI	GCAATG	1 cut(s)	284
BseBI	CCWGG	1 cut(s)	329
BseDI	CCNNGG	2 cut(s)	197, 327
BseGI	GGATG	2 cut(s)	94, 224
BseMI	GCAATG	1 cut(s)	284
BseNI	ACTGG	2 cut(s)	283, 334
BseXI	GCAGC	3 cut(s)	82, 261, 526
BsgI	GTGCAG	2 cut(s)	89, 413
BshFI	GGCC	1 cut(s)	456
BsmI	GAATGC	1 cut(s)	156
BsnI	GGCC	1 cut(s)	456
Bsp1407I	TGTACA	2 cut(s)	76, 263
Bsp143I	GATC	2 cut(s)	115, 300
BspANI	GGCC	1 cut(s)	456
BspHI	TCATGA	1 cut(s)	539
BspLI	GGNNCC	1 cut(s)	455
BsrDI	GCAATG	1 cut(s)	284
BsrGI	TGTACA	2 cut(s)	76, 263
BsrI	ACTGG	2 cut(s)	283, 334
BssECI	CCNNGG	2 cut(s)	197, 327
BssMI	GATC	2 cut(s)	115, 300
Bst2UI	CCWGG	1 cut(s)	329
Bst4CI	ACNGT	1 cut(s)	250
BstAUI	TGTACA	2 cut(s)	76, 263
BstDSI	CCRYGG	1 cut(s)	197
BstF5I	GGATG	2 cut(s)	94, 224
BstKTI	GATC	2 cut(s)	118, 303
BstMBI	GATC	2 cut(s)	115, 300
BstMWI	GCNNNNNNNGC	3 cut(s)	91, 153, 323
BstNI	CCWGG	1 cut(s)	329
BstSCI	CCNGG	1 cut(s)	327
BstSFI	CTRYAG	1 cut(s)	162
BstV1I	GCAGC	3 cut(s)	82, 261, 526
BstV2I	GAAGAC	1 cut(s)	404
BstX2I	RGATCY	1 cut(s)	300
BstXI	CCANNNNNNTGG	1 cut(s)	231
BstYI	RGATCY	1 cut(s)	300
BsuRI	GGCC	1 cut(s)	456
BtgI	CCRYGG	1 cut(s)	197
BtsCI	GGATG	2 cut(s)	94, 224
CciI	TCATGA	1 cut(s)	539
Cfr13I	GGNCC	3 cut(s)	331, 370, 454
Csp6I	GTAC	4 cut(s)	77, 264, 414, 447
CspCI	CAANNNNNGTGG	2 cut(s)	369, 404
CviAII	CATG	3 cut(s)	80, 131, 540
CviJI	RGCY	7 cut(s)	73, 85, 235, 274, 456, 503, 517
CviKI_1	RGCY	7 cut(s)	73, 85, 235, 274, 456, 503, 517
CviQI	GTAC	4 cut(s)	77, 264, 414, 447
DpnI	GATC	2 cut(s)	117, 302
DpnII	GATC	2 cut(s)	115, 300
Eco47I	GGWCC	2 cut(s)	331, 370
EcoRII	CCWGG	1 cut(s)	327
FaeI	CATG	3 cut(s)	83, 134, 543
FalI	AAGNNNNNCTT	2 cut(s)	57, 89
FatI	CATG	3 cut(s)	79, 130, 539
FbaI	TGATCA	1 cut(s)	115
Fnu4HI	GCNGC	3 cut(s)	71, 275, 515
FokI	GGATG	2 cut(s)	101, 211
Fsp4HI	GCNGC	3 cut(s)	71, 275, 515
GluI	GCNGC	3 cut(s)	71, 275, 515
GsuI	CTGGAG	1 cut(s)	275
HaeIII	GGCC	1 cut(s)	456
Hin1II	CATG	3 cut(s)	83, 134, 543
HincII	GTYRAC	2 cut(s)	295, 425
HindII	GTYRAC	2 cut(s)	295, 425
HinfI	GANTC	2 cut(s)	28, 400
HphI	GGTGA	1 cut(s)	536
Hpy166II	GTNNAC	2 cut(s)	295, 425
Hpy188I	TCNGA	1 cut(s)	33
Hpy188III	TCNNGA	4 cut(s)	113, 254, 404, 540
Hpy8I	GTNNAC	2 cut(s)	295, 425
HpyAV	CCTTC	2 cut(s)	28, 361
HpyCH4III	ACNGT	1 cut(s)	250
HpyCH4V	TGCA	5 cut(s)	70, 147, 277, 380, 394
HpyF10VI	GCNNNNNNNGC	3 cut(s)	91, 153, 323
Hsp92II	CATG	3 cut(s)	83, 134, 543
Ksp22I	TGATCA	1 cut(s)	115
Kzo9I	GATC	2 cut(s)	115, 300
LmnI	GCTCC	1 cut(s)	256
LpnPI	CCDG	9 cut(s)	71, 239, 296, 303, 314, 341, 341, 347, 472
Lsp1109I	GCAGC	3 cut(s)	82, 261, 526
MaeIII	GTNAC	2 cut(s)	17, 349
MalI	GATC	2 cut(s)	117, 302
MboI	GATC	2 cut(s)	115, 300
MboII	GAAGA	4 cut(s)	409, 484, 487, 539
MflI	RGATCY	1 cut(s)	300
MluCI	AATT	5 cut(s)	172, 382, 409, 438, 532
MlyI	GAGTC	1 cut(s)	394
MmeI	TCCRAC	1 cut(s)	234
MnlI	CCTC	1 cut(s)	37
MseI	TTAA	2 cut(s)	437, 441
MslI	CAYNNNNRTG	1 cut(s)	229
MspR9I	CCNGG	1 cut(s)	329
Mva1269I	GAATGC	1 cut(s)	156
MvaI	CCWGG	1 cut(s)	329
MwoI	GCNNNNNNNGC	3 cut(s)	91, 153, 323
NdeII	GATC	2 cut(s)	115, 300
NlaIII	CATG	3 cut(s)	83, 134, 543
NlaIV	GGNNCC	1 cut(s)	455
NmuCI	GTSAC	1 cut(s)	349
PacI	TTAATTAA	1 cut(s)	441
PagI	TCATGA	1 cut(s)	539
PctI	GAATGC	1 cut(s)	156
PfeI	GAWTC	1 cut(s)	28
PkrI	GCNGC	3 cut(s)	72, 276, 516
PleI	GAGTC	1 cut(s)	394
PpsI	GAGTC	1 cut(s)	394
PshBI	ATTAAT	2 cut(s)	437, 441
PsiI	TTATAA	1 cut(s)	183
Psp6I	CCWGG	1 cut(s)	327
PspGI	CCWGG	1 cut(s)	327
PspN4I	GGNNCC	1 cut(s)	455
PspPI	GGNCC	3 cut(s)	331, 370, 454
PsuI	RGATCY	1 cut(s)	300
RsaI	GTAC	4 cut(s)	78, 265, 415, 448
RsaNI	GTAC	4 cut(s)	77, 264, 414, 447
RseI	CAYNNNNRTG	1 cut(s)	229
SaqAI	TTAA	2 cut(s)	437, 441
SatI	GCNGC	3 cut(s)	71, 275, 515
Sau3AI	GATC	2 cut(s)	115, 300
Sau96I	GGNCC	3 cut(s)	331, 370, 454
SchI	GAGTC	1 cut(s)	394
ScrFI	CCNGG	1 cut(s)	329
SetI	ASST	7 cut(s)	48, 75, 87, 276, 360, 505, 519
SfcI	CTRYAG	1 cut(s)	162
SinI	GGWCC	2 cut(s)	331, 370
SmiMI	CAYNNNNRTG	1 cut(s)	229
SmlI	CTYRAG	1 cut(s)	41
SmoI	CTYRAG	1 cut(s)	41
Sse9I	AATT	5 cut(s)	172, 382, 409, 438, 532
StyD4I	CCNGG	1 cut(s)	327
TaaI	ACNGT	1 cut(s)	250
TasI	AATT	5 cut(s)	172, 382, 409, 438, 532
TatI	WGTACW	3 cut(s)	76, 263, 413
TfiI	GAWTC	1 cut(s)	28
Tru1I	TTAA	2 cut(s)	437, 441
Tru9I	TTAA	2 cut(s)	437, 441
TseFI	GTSAC	1 cut(s)	349
TseI	GCWGC	3 cut(s)	70, 274, 514
Tsp45I	GTSAC	1 cut(s)	349
TspDTI	ATGAA	2 cut(s)	17, 380
TspGWI	ACGGA	1 cut(s)	186
VpaK11BI	GGWCC	2 cut(s)	331, 370
VspI	ATTAAT	2 cut(s)	437, 441
XapI	RAATTY	1 cut(s)	532
XcmI	CCANNNNNNNNNTGG	1 cut(s)	335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.