Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0022420

Physical Location & Seq

Genomic Coordinates Reverse (-)

197003 .. 199979

Gene Structure 2977 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1002700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGGGTTCCATCGCCATGGAACCCGAGGACATAGCAACCTACGCGGCCCTTCATTCAATTCCTCTCGACAATTCCACCCGCATTTGCACGTCGGTGAACATGATACATTCAAATGGCTTCTTCTTTCACAACAATCCTCTGCAGTATTCACTCCCCCTTCTCCTCATGCAATTCTCTCTCGCTACCGGCGTCATTGTCCTCGCCTCCTTGCTCCTCAAGCCTCTCGGCCAGCCTGTCATCGTCGCGCAAGTCTTAGGGGGTATAATACTAGGTCCTTCGATTCTAGGCCGGAGTGATGCATTTATCGATAGTTTTTTTCCTCTCAGAAGTTTCATACTGCTGGATCTTGTTTCCTCTTTTGGATACATGTTCTGCTTCTTCCTCATTGGAGTACAAACGGATCCCTTCATGGTCAAGAAGATTGACAGAAAGACATTTGCCATTGGCGTTGCGACGTTGATTGTCCCAGTTCTCTTGACTCAAGGATGGTGGTATCTCTTGAACCGCTATGTGAATTTGGATATAGAGGTGGTCAGTTCACTTCCGGCTATGGCTCTAGCAGAGAGTGTGCTGGCCTACCCAACCATTGTTTTCTTTTTGGGTGAGCTTAAGATCGTAAACTCTGATTTTGGGAGGGTGGCAATGTCTTCTTCCATGGTGAGCGGGTTGTGCAGTTTCTCTGTGATAACTGTCGCTGTTCTGGCTGAAGCCGCTAAACCAATGCCGGGTGATGATACCTACGACATGCTAAAAATAATATTCACCGGTGGTGTTGTTGCGCTTGTCATCGTTTTCCTCATTTGGCCAGCACTCAAATGGTTGATAAGGAGCGACTCAGTGGGACAGCCAATGAAGGAACTCCACATTGTTCAACTGCTTGTCGGAGTCTTGGTGACTGGCCTTTTCAGTCATACCACAGGTTTACACACTTACTATGGGCCCTTTGTTCTTGGCTTGACTATACCGGCAGGGCCGCCAGTTGGGTCTGCTCTTGTGGAGAAGCTTGACCTCATCACCTCCTGGATCTTCATGCCCATCTTCTACGTCAAGAATGGCTTGGTCATGGATATCTTCGCCATTCGACTCAAGAATTATACCATCTTCCAATCCACTACCCTCGTGGCTGCACTCGGAAAGTTTTTGGGAGCTTTTCTCAGTTCACTCCTGTGCACCAAAATGCCAGCCACGGATGCTATCTCGCTTGGCCTTGTCATGACCGCGCAAGGCTTTCTCGAACTTGGCTTGTTCAAAATGCTCAAGAGGAATATGGCGGTCTATCATGAAGCATTTGTTGTGATGTGTTTATCCATTATACTTATAACAGGGGGTATCACGCCCATTATAAAACGCCTCTATGATCCTTCGAAGAGGTACATAGTTTACAAGACAAGAACAGTGATGAATGCGCGACCAAACTCCCAGCTCCCCATACTTGTCTGCGTACATGACCAAGATGATGTTGAAAACACAATCAAGCTCCTTGAGGCCTTAAATCCAACCGGAAAAAGCCCTCTTGCCATCTATCTACTCCACCTCATAGAGCTTGTTGGCCGTGCAAACCCTCTTCTCATCCCCCATAAGCTCACAAGAAGGACCTCCTCAAAGGCCAGCCCCTCGGAACAAATTGTCAATGCCTTTAGAAAGTACGAGCAAAGGAACCGGAGCCTTGCCACGGTCCATCCCTTTACCGCAATATCTCACTGTGCCACAATGCACGATGATATCTGTACAATTGCGCTTGATAAAAAGACTTCCCTCATAATAGCTCCATTTTACCCGATGCTCCATGCCAGAAAACAGAGGATGATCAACAAAAATGTTCTGGAAAAAGCACCTTGCTCTGTTGCAATCCTTATTCACCGTGGTGGGCTCTTCAGCAGCAGTTTCAAGAGAAGTTTCAAGAGAAGTTCAAGGGCAATGAAGTTGACAAGCGAGAGCGAGTCTTGTTATAATGTTGCCATTCTCTTCTTTGGAGGACCTGATGACCGAGAGGCAATGGCATTAGGGTCACGAATGGTTGCCCATCCAAACATCAACTTGACGCTGATTCGGATTCTTGTAGACGGAAGTATAATAAATACTAATGAGGACGTGGAGCAGAACTGGCTTGACGACGAGGTGTTGAGTGAGTTCAGGGAAAGCATGACAGACAACTATCGGGTCATGTACATAGAAGAGCTGGTGATGGACGGTTCAGGGACTATGGCTGTGCTCCGTTCCATGCAAAGTAACTACGAACTAGTAATAGCAGGAAGAAATCAAGACAAAGGGTCACCATCCCTGTCAGGTCTTGTGGACTTGGAGGAGCCTAGTGAGTTGGGAGCTATTGGTGAGGTGCTAGCCACCGCAGATTTCATGGGGGAAACCGGAGTTTTGGTGGTGCAACAACACACCAAAGCGGTGAATGACGAATGTGAGAGTCATACAGGAAACTTGAGGGAGACGAACTTGATAATGGATGATGTTGAGGATTGCCAATTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

828

Amino Acids

91.21

Weight (kDa)

6.24

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	65 - 448	7.8e-31	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	505 - 622	1.5e-09	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	651 - 798	5.2e-11	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022420)

Species	Orthologous Gene IDs
malus_domestica	MD17G1002700.v1.1
prunus_persica	Prupe.3G309000_v2.0.a1 Prupe.3G309000_v2.0.a1
pyrus_communis	pycom17g00670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1319, 1343, 1950
AccBSI	CCGCTC	1 cut(s)	663
AccI	GTMKAC	1 cut(s)	2061
AccII	CGCG	4 cut(s)	44, 245, 1220, 1408
AclWI	GGATC	5 cut(s)	351, 395, 408, 1031, 1352
AcoI	YGGCCR	3 cut(s)	226, 803, 1549
AcsI	RAATTY	1 cut(s)	514
AcuI	CTGAAG	2 cut(s)	726, 1858
AcyI	GRCGYC	1 cut(s)	189
AfaI	GTAC	6 cut(s)	393, 1373, 1443, 1646, 1729, 2168
AfiI	CCNNNNNNNGG	3 cut(s)	725, 980, 1672
AflII	CTTAAG	1 cut(s)	608
AflIII	ACRYGT	1 cut(s)	366
AgeI	ACCGGT	1 cut(s)	764
AgsI	TTSAA	9 cut(s)	57, 111, 502, 872, 1249, 1463, 1888, 1900, 1911
AhdI	GACNNNNNGTC	1 cut(s)	2269
AhlI	ACTAGT	1 cut(s)	2239
AjiI	CACGTC	2 cut(s)	90, 2092
AjnI	CCWGG	1 cut(s)	1019
AleI	CACNNNNGTG	3 cut(s)	92, 1165, 1863
Alw21I	GWGCWC	2 cut(s)	1172, 2214
Alw26I	GTCTC	1 cut(s)	2435
Alw44I	GTGCAC	1 cut(s)	1168
AlwI	GGATC	5 cut(s)	351, 395, 408, 1031, 1352
Ama87I	CYCGRG	1 cut(s)	23
ApaI	GGGCCC	1 cut(s)	942
ApaLI	GTGCAC	1 cut(s)	1168
ApeKI	GCWGC	2 cut(s)	1124, 1878
ApoI	RAATTY	1 cut(s)	514
AsiGI	ACCGGT	1 cut(s)	764
AspLEI	GCGC	5 cut(s)	247, 781, 1222, 1408, 1738
AspS9I	GGNCC	8 cut(s)	46, 272, 938, 939, 971, 1593, 1675, 1976
AsuC2I	CCSGG	1 cut(s)	726
AsuII	TTCGAA	1 cut(s)	1364
AsuNHI	GCTAGC	1 cut(s)	2338
AvaI	CYCGRG	1 cut(s)	23
AvaII	GGWCC	4 cut(s)	272, 1593, 1675, 1976
BaeGI	GKGCMC	2 cut(s)	942, 1172
BalI	TGGCCA	1 cut(s)	805
BamHI	GGATCC	1 cut(s)	400
BanII	GRGCYC	2 cut(s)	942, 1872
BauI	CACGAG	1 cut(s)	1118
BbsI	GAAGAC	1 cut(s)	639
Bbv12I	GWGCWC	2 cut(s)	1172, 2214
BbvI	GCAGC	2 cut(s)	1111, 1890
BccI	CCATC	9 cut(s)	17, 480, 1043, 1106, 1526, 1686, 2031, 2179, 2284
BceAI	ACGGC	1 cut(s)	1536
BciT130I	CCWGG	1 cut(s)	1021
BciVI	GTATCC	1 cut(s)	356
BclI	TGATCA	1 cut(s)	1806
BcnI	CCSGG	1 cut(s)	726
BcoDI	GTCTC	1 cut(s)	2435
BcuI	ACTAGT	1 cut(s)	2239
BfaI	CTAG	6 cut(s)	269, 284, 557, 2240, 2310, 2339
BfmI	CTRYAG	1 cut(s)	140
BfrI	CTTAAG	1 cut(s)	608
BfuI	GTATCC	1 cut(s)	356
BisI	GCNGC	5 cut(s)	45, 711, 974, 1125, 1879
BlsI	GCNGC	5 cut(s)	46, 712, 975, 1126, 1880
Bme1390I	CCNGG	2 cut(s)	726, 1021
Bme18I	GGWCC	4 cut(s)	272, 1593, 1675, 1976
BmeRI	GACNNNNNGTC	1 cut(s)	2269
BmeT110I	CYCGRG	1 cut(s)	23
BmgBI	CACGTC	2 cut(s)	90, 2092
BmgT120I	GGNCC	8 cut(s)	46, 272, 938, 939, 971, 1593, 1675, 1976
BmiI	GGNNCC	7 cut(s)	7, 21, 402, 940, 1658, 1664, 2307
BmrFI	CCNGG	2 cut(s)	726, 1021
BmrI	ACTGGG	1 cut(s)	461
BmsI	GCATC	3 cut(s)	286, 1180, 1770
BmtI	GCTAGC	1 cut(s)	2342
BmuI	ACTGGG	1 cut(s)	461
BpiI	GAAGAC	1 cut(s)	639
BplI	GAGNNNNNCTC	2 cut(s)	1137, 1169
Bpu14I	TTCGAA	1 cut(s)	1364
BpuEI	CTTGAG	6 cut(s)	200, 465, 1070, 1241, 1502, 2455
BpuMI	CCSGG	1 cut(s)	726
Bsa29I	ATCGAT	1 cut(s)	306
BsaHI	GRCGYC	1 cut(s)	189
BsaJI	CCNNGG	7 cut(s)	15, 24, 654, 1185, 1614, 1671, 1861
BsaWI	WCCGGW	4 cut(s)	764, 1499, 1659, 2366
BsaXI	ACNNNNNCTCC	4 cut(s)	876, 906, 2361, 2391
Bsc4I	CCNNNNNNNGG	3 cut(s)	725, 980, 1672
Bse118I	RCCGGY	3 cut(s)	185, 764, 964
Bse1I	ACTGG	4 cut(s)	467, 901, 977, 2108
Bse3DI	GCAATG	3 cut(s)	648, 1923, 2001
BseBI	CCWGG	1 cut(s)	1021
BseCI	ATCGAT	1 cut(s)	306
BseDI	CCNNGG	7 cut(s)	15, 24, 654, 1185, 1614, 1671, 1861
BseGI	GGATG	8 cut(s)	491, 1195, 1569, 1678, 1809, 2023, 2276, 2464
BseLI	CCNNNNNNNGG	3 cut(s)	725, 980, 1672
BseMI	GCAATG	3 cut(s)	648, 1923, 2001
BseMII	CTCAG	3 cut(s)	337, 849, 1168
BseNI	ACTGG	4 cut(s)	467, 901, 977, 2108
BseRI	GAGGAG	4 cut(s)	152, 203, 1588, 2318
BseSI	GKGCMC	2 cut(s)	942, 1172
BseXI	GCAGC	2 cut(s)	1111, 1890
BseYI	CCCAGC	1 cut(s)	1419
BsgI	GTGCAG	2 cut(s)	691, 1110
Bsh1236I	CGCG	4 cut(s)	44, 245, 1220, 1408
BshTI	ACCGGT	1 cut(s)	764
BshVI	ATCGAT	1 cut(s)	306
BsiHKAI	GWGCWC	2 cut(s)	1172, 2214
BsiHKCI	CYCGRG	1 cut(s)	23
BsiSI	CCGG	9 cut(s)	186, 289, 545, 725, 765, 965, 1500, 1660, 2367
BslFI	GGGAC	3 cut(s)	449, 855, 2212
BslI	CCNNNNNNNGG	3 cut(s)	725, 980, 1672
BsmAI	GTCTC	1 cut(s)	2435
BsmBI	CGTCTC	1 cut(s)	2435
BsmFI	GGGAC	3 cut(s)	449, 855, 2212
BsmI	GAATGC	1 cut(s)	1408
BsoBI	CYCGRG	1 cut(s)	23
Bsp119I	TTCGAA	1 cut(s)	1364
Bsp120I	GGGCCC	1 cut(s)	938
Bsp1286I	GDGCHC	4 cut(s)	942, 1172, 1872, 2214
Bsp1407I	TGTACA	2 cut(s)	1727, 2166
Bsp143I	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
Bsp19I	CCATGG	2 cut(s)	15, 654
BspCNI	CTCAG	3 cut(s)	336, 848, 1167
BspDI	ATCGAT	1 cut(s)	306
BspFNI	CGCG	4 cut(s)	44, 245, 1220, 1408
BspHI	TCATGA	2 cut(s)	1212, 1279
BspLI	GGNNCC	7 cut(s)	7, 21, 402, 940, 1658, 1664, 2307
BspMAI	CTGCAG	1 cut(s)	144
BspOI	GCTAGC	1 cut(s)	2342
BspPI	GGATC	5 cut(s)	351, 395, 408, 1031, 1352
BspQI	GCTCTTC	2 cut(s)	1877, 2169
BspT104I	TTCGAA	1 cut(s)	1364
BspTI	CTTAAG	1 cut(s)	608
BsrBI	CCGCTC	1 cut(s)	663
BsrDI	GCAATG	3 cut(s)	648, 1923, 2001
BsrFI	RCCGGY	3 cut(s)	185, 764, 964
BsrGI	TGTACA	2 cut(s)	1727, 2166
BsrI	ACTGG	4 cut(s)	467, 901, 977, 2108
BssAI	RCCGGY	3 cut(s)	185, 764, 964
BssECI	CCNNGG	7 cut(s)	15, 24, 654, 1185, 1614, 1671, 1861
BssMI	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
BssNI	GRCGYC	1 cut(s)	189
BssSI	CACGAG	1 cut(s)	1118
BssT1I	CCWWGG	2 cut(s)	15, 654
Bst2BI	CACGAG	1 cut(s)	1118
Bst2UI	CCWGG	1 cut(s)	1021
Bst4CI	ACNGT	6 cut(s)	691, 1396, 1675, 1703, 1862, 2192
Bst6I	CTCTTC	5 cut(s)	1361, 1569, 1877, 1970, 2169
BstACI	GRCGYC	1 cut(s)	189
BstAFI	CTTAAG	1 cut(s)	608
BstAUI	TGTACA	2 cut(s)	1727, 2166
BstBI	TTCGAA	1 cut(s)	1364
BstC8I	GCNNGC	6 cut(s)	230, 573, 807, 1182, 1609, 2340
BstDEI	CTNAG	4 cut(s)	253, 323, 835, 1154
BstDSI	CCRYGG	5 cut(s)	15, 654, 1185, 1671, 1861
BstEII	GGTNACC	1 cut(s)	2271
BstF5I	GGATG	8 cut(s)	491, 1195, 1569, 1678, 1809, 2023, 2276, 2464
BstFNI	CGCG	4 cut(s)	44, 245, 1220, 1408
BstHHI	GCGC	5 cut(s)	247, 781, 1222, 1408, 1738
BstKTI	GATC	6 cut(s)	346, 403, 615, 1026, 1360, 1809
BstMAI	GTCTC	1 cut(s)	2435
BstMBI	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
BstMWI	GCNNNNNNNGC	7 cut(s)	41, 217, 669, 701, 710, 1190, 2104
BstNI	CCWGG	1 cut(s)	1021
BstNSI	RCATGY	2 cut(s)	370, 748
BstPI	GGTNACC	1 cut(s)	2271
BstSCI	CCNGG	2 cut(s)	724, 1019
BstSFI	CTRYAG	1 cut(s)	140
BstSLI	GKGCMC	2 cut(s)	942, 1172
BstUI	CGCG	4 cut(s)	44, 245, 1220, 1408
BstV1I	GCAGC	2 cut(s)	1111, 1890
BstV2I	GAAGAC	1 cut(s)	639
BstX2I	RGATCY	3 cut(s)	343, 400, 1023
BstXI	CCANNNNNNTGG	1 cut(s)	16
BstYI	RGATCY	3 cut(s)	343, 400, 1023
Bsu15I	ATCGAT	1 cut(s)	306
BsuI	GTATCC	1 cut(s)	356
BsuTUI	ATCGAT	1 cut(s)	306
BtgI	CCRYGG	5 cut(s)	15, 654, 1185, 1671, 1861
BtrI	CACGTC	2 cut(s)	90, 2092
BtsCI	GGATG	8 cut(s)	491, 1195, 1569, 1678, 1809, 2023, 2276, 2464
BtsIMutI	CAGTG	3 cut(s)	843, 1401, 1699
Cac8I	GCNNGC	6 cut(s)	230, 573, 807, 1182, 1609, 2340
CciI	TCATGA	2 cut(s)	1212, 1279
CfoI	GCGC	5 cut(s)	247, 781, 1222, 1408, 1738
Cfr10I	RCCGGY	3 cut(s)	185, 764, 964
Cfr13I	GGNCC	8 cut(s)	46, 272, 938, 939, 971, 1593, 1675, 1976
ClaI	ATCGAT	1 cut(s)	306
CseI	GACGC	2 cut(s)	178, 2050
Csp6I	GTAC	6 cut(s)	392, 1372, 1442, 1645, 1728, 2167
CspAI	ACCGGT	1 cut(s)	764
CspCI	CAANNNNNGTGG	2 cut(s)	470, 505
CviQI	GTAC	6 cut(s)	392, 1372, 1442, 1645, 1728, 2167
DdeI	CTNAG	4 cut(s)	253, 323, 835, 1154
DpnI	GATC	6 cut(s)	345, 402, 614, 1025, 1359, 1808
DpnII	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
DriI	GACNNNNNGTC	1 cut(s)	2269
EaeI	YGGCCR	3 cut(s)	226, 803, 1549
Eam1104I	CTCTTC	5 cut(s)	1361, 1569, 1877, 1970, 2169
Eam1105I	GACNNNNNGTC	1 cut(s)	2269
EarI	CTCTTC	5 cut(s)	1361, 1569, 1877, 1970, 2169
Eco130I	CCWWGG	2 cut(s)	15, 654
Eco147I	AGGCCT	1 cut(s)	1487
Eco24I	GRGCYC	2 cut(s)	942, 1872
Eco32I	GATATC	2 cut(s)	1069, 1723
Eco47I	GGWCC	4 cut(s)	272, 1593, 1675, 1976
Eco57I	CTGAAG	2 cut(s)	726, 1858
Eco88I	CYCGRG	1 cut(s)	23
Eco91I	GGTNACC	1 cut(s)	2271
EcoO109I	RGGNCCY	4 cut(s)	272, 939, 1593, 1976
EcoO65I	GGTNACC	1 cut(s)	2271
EcoRII	CCWGG	1 cut(s)	1019
EcoRV	GATATC	2 cut(s)	1069, 1723
EcoT14I	CCWWGG	2 cut(s)	15, 654
EcoT22I	ATGCAT	1 cut(s)	301
EcoT38I	GRGCYC	2 cut(s)	942, 1872
ErhI	CCWWGG	2 cut(s)	15, 654
Esp3I	CGTCTC	1 cut(s)	2435
FalI	AAGNNNNNCTT	2 cut(s)	1040, 1072
FaqI	GGGAC	3 cut(s)	449, 855, 2212
FauI	CCCGC	2 cut(s)	86, 656
FbaI	TGATCA	1 cut(s)	1806
FblI	GTMKAC	1 cut(s)	2061
Fnu4HI	GCNGC	5 cut(s)	45, 711, 974, 1125, 1879
FokI	GGATG	8 cut(s)	498, 1202, 1556, 1665, 1816, 2010, 2263, 2471
FriOI	GRGCYC	2 cut(s)	942, 1872
Fsp4HI	GCNGC	5 cut(s)	45, 711, 974, 1125, 1879
FspBI	CTAG	6 cut(s)	269, 284, 557, 2240, 2310, 2339
GlaI	GCGC	5 cut(s)	246, 780, 1221, 1407, 1737
GluI	GCNGC	5 cut(s)	45, 711, 974, 1125, 1879
GsaI	CCCAGC	1 cut(s)	1423
HapII	CCGG	9 cut(s)	186, 289, 545, 725, 765, 965, 1500, 1660, 2367
HgaI	GACGC	2 cut(s)	178, 2050
HhaI	GCGC	5 cut(s)	247, 781, 1222, 1408, 1738
Hin1I	GRCGYC	1 cut(s)	189
Hin6I	GCGC	5 cut(s)	245, 779, 1220, 1406, 1736
HinP1I	GCGC	5 cut(s)	245, 779, 1220, 1406, 1736
HincII	GTYRAC	1 cut(s)	1926
HindII	GTYRAC	1 cut(s)	1926
HindIII	AAGCTT	1 cut(s)	1001
HinfI	GANTC	9 cut(s)	280, 478, 833, 885, 1083, 1940, 2047, 2053, 2419
HpaII	CCGG	9 cut(s)	186, 289, 545, 725, 765, 965, 1500, 1660, 2367
Hpy188I	TCNGA	6 cut(s)	326, 625, 884, 1133, 1618, 2052
Hpy99I	CGWCG	4 cut(s)	94, 245, 457, 2117
HpyAV	CCTTC	7 cut(s)	59, 167, 285, 415, 847, 1371, 1584
HpyCH4III	ACNGT	6 cut(s)	691, 1396, 1675, 1703, 1862, 2192
HpyCH4IV	ACGT	4 cut(s)	89, 455, 1044, 2091
HpyF10VI	GCNNNNNNNGC	7 cut(s)	41, 217, 669, 701, 710, 1190, 2104
HpyF3I	CTNAG	4 cut(s)	253, 323, 835, 1154
HpySE526I	ACGT	4 cut(s)	89, 455, 1044, 2091
Hsp92I	GRCGYC	1 cut(s)	189
HspAI	GCGC	5 cut(s)	245, 779, 1220, 1406, 1736
Ksp22I	TGATCA	1 cut(s)	1806
Kzo9I	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
LguI	GCTCTTC	2 cut(s)	1877, 2169
Lsp1109I	GCAGC	2 cut(s)	1111, 1890
LweI	GCATC	3 cut(s)	286, 1180, 1770
MaeI	CTAG	6 cut(s)	269, 284, 557, 2240, 2310, 2339
MaeII	ACGT	4 cut(s)	89, 455, 1044, 2091
MaeIII	GTNAC	4 cut(s)	892, 2007, 2228, 2271
MalI	GATC	6 cut(s)	345, 402, 614, 1025, 1359, 1808
MbiI	CCGCTC	1 cut(s)	663
MboI	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
MfeI	CAATTG	1 cut(s)	1731
MflI	RGATCY	3 cut(s)	343, 400, 1023
MhlI	GDGCHC	4 cut(s)	942, 1172, 1872, 2214
MlsI	TGGCCA	1 cut(s)	805
MluCI	AATT	8 cut(s)	57, 70, 170, 514, 1090, 1623, 1731, 2477
MluNI	TGGCCA	1 cut(s)	805
MlyI	GAGTC	6 cut(s)	472, 827, 894, 1077, 1949, 2428
MmeI	TCCRAC	2 cut(s)	862, 1520
Mox20I	TGGCCA	1 cut(s)	805
Mph1103I	ATGCAT	1 cut(s)	301
MscI	TGGCCA	1 cut(s)	805
MseI	TTAA	2 cut(s)	609, 1490
MslI	CAYNNNNRTG	7 cut(s)	14, 92, 111, 814, 1165, 1175, 1863
Msp20I	TGGCCA	1 cut(s)	805
MspCI	CTTAAG	1 cut(s)	608
MspI	CCGG	9 cut(s)	186, 289, 545, 725, 765, 965, 1500, 1660, 2367
MspR9I	CCNGG	2 cut(s)	726, 1021
MunI	CAATTG	1 cut(s)	1731
Mva1269I	GAATGC	1 cut(s)	1408
MvaI	CCWGG	1 cut(s)	1021
MvnI	CGCG	4 cut(s)	44, 245, 1220, 1408
MwoI	GCNNNNNNNGC	7 cut(s)	41, 217, 669, 701, 710, 1190, 2104
NciI	CCSGG	1 cut(s)	726
NcoI	CCATGG	2 cut(s)	15, 654
NdeII	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
NheI	GCTAGC	1 cut(s)	2338
NlaIV	GGNNCC	7 cut(s)	7, 21, 402, 940, 1658, 1664, 2307
NmeAIII	GCCGAG	1 cut(s)	204
NmuCI	GTSAC	3 cut(s)	892, 2007, 2271
NsiI	ATGCAT	1 cut(s)	301
NspI	RCATGY	2 cut(s)	370, 748
NspV	TTCGAA	1 cut(s)	1364
OliI	CACNNNNGTG	3 cut(s)	92, 1165, 1863
PagI	TCATGA	2 cut(s)	1212, 1279
PceI	AGGCCT	1 cut(s)	1487
PciI	ACATGT	1 cut(s)	366
PciSI	GCTCTTC	2 cut(s)	1877, 2169
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	1 cut(s)	1408
PfeI	GAWTC	3 cut(s)	280, 2047, 2053
PfoI	TCCNGGA	1 cut(s)	1019
PinAI	ACCGGT	1 cut(s)	764
PkrI	GCNGC	5 cut(s)	46, 712, 975, 1126, 1880
PleI	GAGTC	6 cut(s)	472, 827, 893, 1077, 1948, 2427
PpsI	GAGTC	6 cut(s)	472, 827, 893, 1077, 1948, 2427
PpuMI	RGGWCCY	3 cut(s)	272, 1593, 1976
PscI	ACATGT	1 cut(s)	366
PsiI	TTATAA	3 cut(s)	1319, 1343, 1950
Psp5II	RGGWCCY	3 cut(s)	272, 1593, 1976
Psp6I	CCWGG	1 cut(s)	1019
PspEI	GGTNACC	1 cut(s)	2271
PspFI	CCCAGC	1 cut(s)	1419
PspGI	CCWGG	1 cut(s)	1019
PspN4I	GGNNCC	7 cut(s)	7, 21, 402, 940, 1658, 1664, 2307
PspOMI	GGGCCC	1 cut(s)	938
PspPI	GGNCC	8 cut(s)	46, 272, 938, 939, 971, 1593, 1675, 1976
PspPPI	RGGWCCY	3 cut(s)	272, 1593, 1976
PstI	CTGCAG	1 cut(s)	144
PsuI	RGATCY	3 cut(s)	343, 400, 1023
RsaI	GTAC	6 cut(s)	393, 1373, 1443, 1646, 1729, 2168
RsaNI	GTAC	6 cut(s)	392, 1372, 1442, 1645, 1728, 2167
RseI	CAYNNNNRTG	7 cut(s)	14, 92, 111, 814, 1165, 1175, 1863
SapI	GCTCTTC	2 cut(s)	1877, 2169
SaqAI	TTAA	2 cut(s)	609, 1490
SatI	GCNGC	5 cut(s)	45, 711, 974, 1125, 1879
Sau3AI	GATC	6 cut(s)	343, 400, 612, 1023, 1357, 1806
Sau96I	GGNCC	8 cut(s)	46, 272, 938, 939, 971, 1593, 1675, 1976
SchI	GAGTC	6 cut(s)	472, 827, 894, 1077, 1949, 2428
ScrFI	CCNGG	2 cut(s)	726, 1021
SduI	GDGCHC	4 cut(s)	942, 1172, 1872, 2214
SfaNI	GCATC	3 cut(s)	286, 1180, 1770
SfcI	CTRYAG	1 cut(s)	140
SfuI	TTCGAA	1 cut(s)	1364
SgrAI	CRCCGGYG	1 cut(s)	764
SinI	GGWCC	4 cut(s)	272, 1593, 1675, 1976
SmiMI	CAYNNNNRTG	7 cut(s)	14, 92, 111, 814, 1165, 1175, 1863
SmlI	CTYRAG	7 cut(s)	215, 480, 608, 1085, 1256, 1481, 2434
SmoI	CTYRAG	7 cut(s)	215, 480, 608, 1085, 1256, 1481, 2434
SpeI	ACTAGT	1 cut(s)	2239
Sse9I	AATT	8 cut(s)	57, 70, 170, 514, 1090, 1623, 1731, 2477
SseBI	AGGCCT	1 cut(s)	1487
SspI	AATATT	1 cut(s)	759
SspMI	CTAG	6 cut(s)	269, 284, 557, 2240, 2310, 2339
StuI	AGGCCT	1 cut(s)	1487
StyD4I	CCNGG	2 cut(s)	724, 1019
StyI	CCWWGG	2 cut(s)	15, 654
TaaI	ACNGT	6 cut(s)	691, 1396, 1675, 1703, 1862, 2192
TaiI	ACGT	4 cut(s)	92, 458, 1047, 2094
TaqI	TCGA	6 cut(s)	66, 278, 306, 1081, 1233, 1364
TaqII	GACCGA	1 cut(s)	2001
TasI	AATT	8 cut(s)	57, 70, 170, 514, 1090, 1623, 1731, 2477
TatI	WGTACW	3 cut(s)	391, 1727, 2166
TauI	GCSGC	3 cut(s)	47, 713, 976
TfiI	GAWTC	3 cut(s)	280, 2047, 2053
Tru1I	TTAA	2 cut(s)	609, 1490
Tru9I	TTAA	2 cut(s)	609, 1490
TscAI	CASTG	3 cut(s)	843, 1401, 1706
TseFI	GTSAC	3 cut(s)	892, 2007, 2271
TseI	GCWGC	2 cut(s)	1124, 1878
Tsp45I	GTSAC	3 cut(s)	892, 2007, 2271
TspDTI	ATGAA	9 cut(s)	41, 322, 397, 866, 1018, 1296, 1415, 1934, 2344
TspGWI	ACGGA	4 cut(s)	413, 1202, 2079, 2204
TspRI	CASTG	3 cut(s)	843, 1401, 1706
Vha464I	CTTAAG	1 cut(s)	608
VneI	GTGCAC	1 cut(s)	1168
VpaK11BI	GGWCC	4 cut(s)	272, 1593, 1675, 1976
XapI	RAATTY	1 cut(s)	514
XceI	RCATGY	2 cut(s)	370, 748
XcmI	CCANNNNNNNNNTGG	2 cut(s)	813, 1117
XmiI	GTMKAC	1 cut(s)	2061
XspI	CTAG	6 cut(s)	269, 284, 557, 2240, 2310, 2339
Zsp2I	ATGCAT	1 cut(s)	301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.