MD17G1124600.v1.1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0028766
Physical Location & Seq
Forward (+)
10847179 .. 10848144
966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1124600.v1.1.491

Sequence Viewer

Length: 237 bp
ATGGTTGACTTTGGCAAGAAACAGCACCGTACCCTAAACATTACTGTTGATCTTGCTTCCATACACATTGATGCCCTTGATGTAGTCAGCGGGAATGGCCATGAGCATGCAATCGTGTACATTGGAACATCCCAAGGAGTGTATGGCGCTCGGGCGACAATCAATGTGTGGTCCCCTTTGATCGAACACGTAAACGAGTTCAGTTTCGTACACATCCAGTTCTCAATTACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

79

Amino Acids

8.65

Weight (kDa)

6.1

Isoelectric Point (pI)

27.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028766)

Species Orthologous Gene IDs
malus_domestica MD17G1124600.v1.1
pyrus_communis pycom12g03900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 90
AcoI YGGCCR 1 cut(s) 97
AfaI GTAC 3 cut(s) 31, 119, 210
AflIII ACRYGT 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 1 cut(s) 97
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 150
Bme18I GGWCC 1 cut(s) 171
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 1 cut(s) 173
BmsI GCATC 1 cut(s) 61
BsaAI YACGTR 1 cut(s) 190
BsaJI CCNNGG 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 2 cut(s) 128, 213
BseNI ACTGG 1 cut(s) 217
BshFI GGCC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 157
BsnI GGCC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 150
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 2 cut(s) 49, 180
BspACI CCGC 1 cut(s) 90
BspANI GGCC 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 49, 180
BssT1I CCWWGG 1 cut(s) 133
Bst4CI ACNGT 2 cut(s) 29, 46
BstAUI TGTACA 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 108
BstF5I GGATG 2 cut(s) 128, 213
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 2 cut(s) 52, 183
BstMBI GATC 2 cut(s) 49, 180
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNSI RCATGY 1 cut(s) 110
BsuRI GGCC 1 cut(s) 99
BtsCI GGATG 2 cut(s) 128, 213
Cac8I GCNNGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 171
Csp6I GTAC 3 cut(s) 30, 118, 209
CviAII CATG 2 cut(s) 101, 107
CviJI RGCY 1 cut(s) 99
CviKI_1 RGCY 1 cut(s) 99
CviQI GTAC 3 cut(s) 30, 118, 209
DpnI GATC 2 cut(s) 51, 182
DpnII GATC 2 cut(s) 49, 180
EaeI YGGCCR 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 2 cut(s) 104, 110
FaiI YATR 4 cut(s) 62, 102, 108, 144
FaqI GGGAC 1 cut(s) 157
FatI CATG 2 cut(s) 100, 106
FauI CCCGC 1 cut(s) 83
FokI GGATG 2 cut(s) 115, 200
GlaI GCGC 1 cut(s) 148
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 1 cut(s) 99
HhaI GCGC 1 cut(s) 149
Hin1II CATG 2 cut(s) 104, 110
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
Hpy166II GTNNAC 4 cut(s) 7, 118, 193, 211
Hpy8I GTNNAC 4 cut(s) 7, 118, 193, 211
HpyCH4III ACNGT 2 cut(s) 29, 46
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 1 cut(s) 110
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 2 cut(s) 104, 110
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 2 cut(s) 49, 180
LpnPI CCDG 1 cut(s) 230
LweI GCATC 1 cut(s) 61
MaeII ACGT 1 cut(s) 189
MalI GATC 2 cut(s) 51, 182
MboI GATC 2 cut(s) 49, 180
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 99
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MslI CAYNNNNRTG 2 cut(s) 69, 105
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 90
MwoI GCNNNNNNNGC 1 cut(s) 96
NdeII GATC 2 cut(s) 49, 180
NlaIII CATG 2 cut(s) 104, 110
NlaIV GGNNCC 1 cut(s) 173
NspI RCATGY 1 cut(s) 110
PaeI GCATGC 1 cut(s) 110
Ppu21I YACGTR 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 173
PspPI GGNCC 1 cut(s) 171
RsaI GTAC 3 cut(s) 31, 119, 210
RsaNI GTAC 3 cut(s) 30, 118, 209
RseI CAYNNNNRTG 2 cut(s) 69, 105
Sau3AI GATC 2 cut(s) 49, 180
Sau96I GGNCC 1 cut(s) 171
SetI ASST 1 cut(s) 192
SfaNI GCATC 1 cut(s) 61
SinI GGWCC 1 cut(s) 171
SmiMI CAYNNNNRTG 2 cut(s) 69, 105
SphI GCATGC 1 cut(s) 110
Sse9I AATT 1 cut(s) 225
SsiI CCGC 1 cut(s) 90
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 29, 46
TaiI ACGT 1 cut(s) 192
TaqI TCGA 1 cut(s) 183
TasI AATT 1 cut(s) 225
TatI WGTACW 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 171
XceI RCATGY 1 cut(s) 110
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.