MD17G1141900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0028775
Physical Location & Seq
Reverse (-)
12769914 .. 12773276
3363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1141900.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGAACCCGTGCCACAGCAACAGCAGCAATCAGCAGGAGGGCCCCGCTTCACTCTGCTGAAGCAGTCGTCGCTGGCGCCGGACCGGGACGACTCGGTGACGCCCGAGCCGGTTGACCCGCGGGTGCGGCTCATGTACATGGCCAACGAGGGCGACTTGGACGCCATTCGAGAGCTCATCGACTCCGGCACCAATGTCAATTTCACTGACATCGACGGCCGCACCGCCCTCCACGTCGCCGCTTGCCAGGGCCAAACCGACGTCGTCCAGCTCTTGCTCCAAAGAGGCGCCGTGGTCGACCCGCGTGACTGTTGGGGCAGCACGCCTCTTGCAGATGCAATTTATTACAAAAACGATGATGTGATCAAACTTTTGGAGGATTACGGTGCTAAACCTCCGATGGCTCCAATGCATGTCCAGAACTCACGGGAAGTCCCCGAATACGAGATTAATCCTAATGAGCTTGATTTCTCCAATAGTGTCGAGATAACCAAGGGAACCTATCGTATTGCATCGTGGCGTGGAATTCAAGTTGCAGTTAAAACGCTTGGGGAGAAAGTATTTGCTGATGAAGATAAAGTAAATGCATTCAGGGATGAGCTTTCTCTACTTCAGAAGATACGGCATCCAAACGTTGTCCAATTTTTGGGTGCTGTAACACAAAGCAGTCCAATGATGATTGTCATAGAATATCTATCCAAGGGAGATTTTCGTGCGTATCTTAAAAGGAAAGGTGCATTAAAACCACCATCAGCATTAAAGTTTTCACTCGACATTGCTAGGGGGATGAATTACTTGCATGAGCATAAACCTGAAGCAATAATTCATCGAGACCTGGAGCCCTCGTATGCTCTCGCACTCTCTCTCTTTATATTTATTGTTGCTGTTATTTTTGCTAGTAGAGAAATAGATGTTTACCAACATAATTTATATCTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Pkinase_Tyr

Protein Analysis

314

Amino Acids

35.14

Weight (kDa)

5.33

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 44 - 132 1.1e-18 Ankyrin repeats (3 copies)
Ank_4 PF13637 46 - 93 4.9e-09 Ankyrin repeats (many copies)
Ank PF00023 72 - 103 1.7e-08 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 162 - 279 4.3e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 282 4e-20 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028775)

Species Orthologous Gene IDs
malus_domestica MD17G1141900.v1.1
pyrus_communis pycom17g13520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccB1I GGYRCC 3 cut(s) 76, 188, 287
AccB7I CCANNNNNTGG 1 cut(s) 646
AccI GTMKAC 1 cut(s) 297
AccII CGCG 2 cut(s) 121, 304
AciI CCGC 8 cut(s) 46, 119, 121, 127, 220, 225, 240, 302
AclI AACGTT 1 cut(s) 633
AcoI YGGCCR 2 cut(s) 141, 217
AcsI RAATTY 1 cut(s) 525
AcuI CTGAAG 3 cut(s) 80, 596, 834
AcyI GRCGYC 5 cut(s) 77, 101, 162, 261, 288
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 646
AgsI TTSAA 1 cut(s) 530
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 2 cut(s) 246, 834
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 4 cut(s) 175, 271, 463, 601
AluI AGCT 4 cut(s) 175, 271, 463, 601
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 1 cut(s) 825
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 4 cut(s) 41, 141, 217, 250
ApaI GGGCCC 1 cut(s) 45
ApeKI GCWGC 2 cut(s) 25, 318
ApoI RAATTY 1 cut(s) 525
AseI ATTAAT 1 cut(s) 450
AspLEI GCGC 2 cut(s) 79, 290
AspS9I GGNCC 4 cut(s) 41, 42, 82, 250
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 45
BalI TGGCCA 1 cut(s) 143
BanI GGYRCC 3 cut(s) 76, 188, 287
BanII GRGCYC 3 cut(s) 45, 177, 843
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 37, 330
BccI CCATC 2 cut(s) 394, 757
BceAI ACGGC 3 cut(s) 232, 275, 638
BciT130I CCWGG 2 cut(s) 248, 836
BclI TGATCA 1 cut(s) 363
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 825
BfaI CTAG 2 cut(s) 780, 897
BfoI RGCGCY 2 cut(s) 80, 291
BisI GCNGC 5 cut(s) 26, 128, 220, 240, 319
BlsI GCNGC 5 cut(s) 27, 129, 221, 241, 320
Bme1390I CCNGG 3 cut(s) 86, 248, 836
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 4 cut(s) 41, 42, 82, 250
BmiI GGNNCC 9 cut(s) 6, 43, 44, 78, 190, 289, 405, 499, 840
BmrFI CCNGG 3 cut(s) 86, 248, 836
BmsI GCATC 3 cut(s) 325, 521, 634
BpmI CTGGAG 1 cut(s) 857
BpuMI CCSGG 1 cut(s) 86
BsaHI GRCGYC 5 cut(s) 77, 101, 162, 261, 288
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 5 cut(s) 119, 247, 291, 492, 699
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 1 cut(s) 646
Bse118I RCCGGY 1 cut(s) 109
Bse3DI GCAATG 1 cut(s) 774
BseBI CCWGG 2 cut(s) 248, 836
BseDI CCNNGG 5 cut(s) 119, 247, 291, 492, 699
BseGI GGATG 3 cut(s) 601, 625, 792
BseLI CCNNNNNNNGG 1 cut(s) 646
BseMI GCAATG 1 cut(s) 774
BseSI GKGCMC 1 cut(s) 45
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 37, 330
Bsh1236I CGCG 2 cut(s) 121, 304
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 4 cut(s) 43, 143, 219, 252
BshNI GGYRCC 3 cut(s) 76, 188, 287
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 4 cut(s) 80, 85, 110, 186
BslFI GGGAC 2 cut(s) 101, 419
BslI CCNNNNNNNGG 1 cut(s) 646
BsmAI GTCTC 1 cut(s) 825
BsmFI GGGAC 2 cut(s) 101, 419
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 4 cut(s) 43, 143, 219, 252
Bso31I GGTCTC 1 cut(s) 825
BsoBI CYCGRG 1 cut(s) 104
Bsp120I GGGCCC 1 cut(s) 41
Bsp1286I GDGCHC 3 cut(s) 45, 177, 843
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 1 cut(s) 363
BspACI CCGC 8 cut(s) 46, 119, 121, 127, 220, 225, 240, 302
BspANI GGCC 4 cut(s) 43, 143, 219, 252
BspFNI CGCG 2 cut(s) 121, 304
BspLI GGNNCC 9 cut(s) 6, 43, 44, 78, 190, 289, 405, 499, 840
BspT107I GGYRCC 3 cut(s) 76, 188, 287
BspTNI GGTCTC 1 cut(s) 825
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 109
BsrGI TGTACA 1 cut(s) 135
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 5 cut(s) 119, 247, 291, 492, 699
BssMI GATC 1 cut(s) 363
BssNI GRCGYC 5 cut(s) 77, 101, 162, 261, 288
BssT1I CCWWGG 2 cut(s) 492, 699
Bst2UI CCWGG 2 cut(s) 248, 836
Bst4CI ACNGT 2 cut(s) 311, 386
BstACI GRCGYC 5 cut(s) 77, 101, 162, 261, 288
BstAUI TGTACA 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 75, 244, 323
BstDSI CCRYGG 2 cut(s) 119, 291
BstF5I GGATG 3 cut(s) 601, 625, 792
BstFNI CGCG 2 cut(s) 121, 304
BstH2I RGCGCY 2 cut(s) 80, 291
BstHHI GCGC 2 cut(s) 79, 290
BstKTI GATC 1 cut(s) 366
BstMAI GTCTC 1 cut(s) 825
BstMBI GATC 1 cut(s) 363
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 25, 70, 127
BstNI CCWGG 2 cut(s) 248, 836
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 84, 246, 834
BstSLI GKGCMC 1 cut(s) 45
BstUI CGCG 2 cut(s) 121, 304
BstV1I GCAGC 2 cut(s) 37, 330
BstZI CGGCCG 1 cut(s) 217
BsuRI GGCC 4 cut(s) 43, 143, 219, 252
BtgI CCRYGG 2 cut(s) 119, 291
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 3 cut(s) 601, 625, 792
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 75, 244, 323
CfoI GCGC 2 cut(s) 79, 290
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 4 cut(s) 41, 42, 82, 250
Cfr42I CCGCGG 1 cut(s) 122
CpoI CGGWCCG 1 cut(s) 82
CseI GACGC 2 cut(s) 109, 170
Csp6I GTAC 1 cut(s) 136
CspI CGGWCCG 1 cut(s) 82
CviAII CATG 4 cut(s) 133, 139, 413, 800
CviQI GTAC 1 cut(s) 136
DinI GGCGCC 2 cut(s) 78, 289
DpnI GATC 1 cut(s) 365
DpnII GATC 1 cut(s) 363
EaeI YGGCCR 2 cut(s) 141, 217
EagI CGGCCG 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 175
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 492, 699
Eco24I GRGCYC 3 cut(s) 45, 177, 843
Eco31I GGTCTC 1 cut(s) 825
Eco47I GGWCC 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 3 cut(s) 80, 596, 834
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 41, 42
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 2 cut(s) 246, 834
EcoT14I CCWWGG 2 cut(s) 492, 699
EcoT22I ATGCAT 2 cut(s) 414, 589
EcoT38I GRGCYC 3 cut(s) 45, 177, 843
EgeI GGCGCC 2 cut(s) 78, 289
EheI GGCGCC 2 cut(s) 78, 289
ErhI CCWWGG 2 cut(s) 492, 699
FaeI CATG 4 cut(s) 136, 142, 416, 803
FaqI GGGAC 2 cut(s) 101, 419
FatI CATG 4 cut(s) 132, 138, 412, 799
FauI CCCGC 4 cut(s) 53, 114, 126, 309
FbaI TGATCA 1 cut(s) 363
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 5 cut(s) 26, 128, 220, 240, 319
FokI GGATG 3 cut(s) 608, 612, 799
FriOI GRGCYC 3 cut(s) 45, 177, 843
Fsp4HI GCNGC 5 cut(s) 26, 128, 220, 240, 319
FspBI CTAG 2 cut(s) 780, 897
GlaI GCGC 2 cut(s) 78, 289
GluI GCNGC 5 cut(s) 26, 128, 220, 240, 319
GsuI CTGGAG 1 cut(s) 857
HaeII RGCGCY 2 cut(s) 80, 291
HaeIII GGCC 4 cut(s) 43, 143, 219, 252
HapII CCGG 4 cut(s) 80, 85, 110, 186
HgaI GACGC 2 cut(s) 109, 170
HhaI GCGC 2 cut(s) 79, 290
Hin1I GRCGYC 5 cut(s) 77, 101, 162, 261, 288
Hin1II CATG 4 cut(s) 136, 142, 416, 803
Hin6I GCGC 2 cut(s) 77, 288
HinP1I GCGC 2 cut(s) 77, 288
HincII GTYRAC 2 cut(s) 115, 298
HindII GTYRAC 2 cut(s) 115, 298
HinfI GANTC 2 cut(s) 92, 182
HpaII CCGG 4 cut(s) 80, 85, 110, 186
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 3 cut(s) 115, 298, 916
Hpy188I TCNGA 3 cut(s) 399, 615, 941
Hpy188III TCNNGA 4 cut(s) 170, 418, 484, 830
Hpy8I GTNNAC 3 cut(s) 115, 298, 916
Hpy99I CGWCG 5 cut(s) 73, 218, 239, 263, 266
HpyCH4III ACNGT 2 cut(s) 311, 386
HpyCH4IV ACGT 3 cut(s) 234, 261, 633
HpyCH4V TGCA 8 cut(s) 332, 338, 412, 512, 536, 587, 737, 799
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 70, 127
HpySE526I ACGT 3 cut(s) 234, 261, 633
Hsp92I GRCGYC 5 cut(s) 77, 101, 162, 261, 288
Hsp92II CATG 4 cut(s) 136, 142, 416, 803
HspAI GCGC 2 cut(s) 77, 288
KasI GGCGCC 2 cut(s) 76, 287
Ksp22I TGATCA 1 cut(s) 363
KspI CCGCGG 1 cut(s) 122
Kzo9I GATC 1 cut(s) 363
LmnI GCTCC 3 cut(s) 282, 409, 838
Lsp1109I GCAGC 2 cut(s) 37, 330
LweI GCATC 3 cut(s) 325, 521, 634
MaeI CTAG 2 cut(s) 780, 897
MaeII ACGT 3 cut(s) 234, 261, 633
MaeIII GTNAC 3 cut(s) 97, 305, 655
MalI GATC 1 cut(s) 365
MboI GATC 1 cut(s) 363
MboII GAAGA 2 cut(s) 584, 628
MhlI GDGCHC 3 cut(s) 45, 177, 843
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 7 cut(s) 199, 339, 525, 641, 790, 822, 925
MluNI TGGCCA 1 cut(s) 143
Mly113I GGCGCC 2 cut(s) 77, 288
MlyI GAGTC 2 cut(s) 86, 176
MnlI CCTC 8 cut(s) 32, 142, 239, 278, 336, 370, 405, 853
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 2 cut(s) 414, 589
MscI TGGCCA 1 cut(s) 143
MseI TTAA 5 cut(s) 450, 540, 723, 740, 758
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 4 cut(s) 80, 85, 110, 186
MspR9I CCNGG 3 cut(s) 86, 248, 836
Mva1269I GAATGC 1 cut(s) 587
MvaI CCWGG 2 cut(s) 248, 836
MvnI CGCG 2 cut(s) 121, 304
MwoI GCNNNNNNNGC 3 cut(s) 25, 70, 127
NarI GGCGCC 2 cut(s) 77, 288
NciI CCSGG 1 cut(s) 86
NdeII GATC 1 cut(s) 363
NlaIII CATG 4 cut(s) 136, 142, 416, 803
NlaIV GGNNCC 9 cut(s) 6, 43, 44, 78, 190, 289, 405, 499, 840
NmuCI GTSAC 2 cut(s) 97, 305
NsiI ATGCAT 2 cut(s) 414, 589
NspI RCATGY 1 cut(s) 416
PctI GAATGC 1 cut(s) 587
PflFI GACNNNGTC 1 cut(s) 263
PflMI CCANNNNNTGG 1 cut(s) 646
PkrI GCNGC 5 cut(s) 27, 129, 221, 241, 320
PleI GAGTC 2 cut(s) 86, 176
PluTI GGCGCC 2 cut(s) 80, 291
PpsI GAGTC 2 cut(s) 86, 176
PshBI ATTAAT 1 cut(s) 450
Psp124BI GAGCTC 1 cut(s) 177
Psp1406I AACGTT 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 246, 834
PspGI CCWGG 2 cut(s) 246, 834
PspN4I GGNNCC 9 cut(s) 6, 43, 44, 78, 190, 289, 405, 499, 840
PspOMI GGGCCC 1 cut(s) 41
PspPI GGNCC 4 cut(s) 41, 42, 82, 250
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 137
Rsr2I CGGWCCG 1 cut(s) 82
RsrII CGGWCCG 1 cut(s) 82
SacI GAGCTC 1 cut(s) 177
SacII CCGCGG 1 cut(s) 122
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 5 cut(s) 450, 540, 723, 740, 758
SatI GCNGC 5 cut(s) 26, 128, 220, 240, 319
Sau3AI GATC 1 cut(s) 363
Sau96I GGNCC 4 cut(s) 41, 42, 82, 250
SchI GAGTC 2 cut(s) 86, 176
ScrFI CCNGG 3 cut(s) 86, 248, 836
SduI GDGCHC 3 cut(s) 45, 177, 843
SfaNI GCATC 3 cut(s) 325, 521, 634
SfoI GGCGCC 2 cut(s) 78, 289
Sfr303I CCGCGG 1 cut(s) 122
SgrBI CCGCGG 1 cut(s) 122
SinI GGWCC 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 137
Sse9I AATT 7 cut(s) 199, 339, 525, 641, 790, 822, 925
SsiI CCGC 8 cut(s) 46, 119, 121, 127, 220, 225, 240, 302
SspDI GGCGCC 2 cut(s) 76, 287
SspMI CTAG 2 cut(s) 780, 897
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 3 cut(s) 84, 246, 834
StyI CCWWGG 2 cut(s) 492, 699
TaaI ACNGT 2 cut(s) 311, 386
TaiI ACGT 3 cut(s) 237, 264, 636
TaqI TCGA 7 cut(s) 169, 180, 213, 297, 483, 771, 829
TasI AATT 7 cut(s) 199, 339, 525, 641, 790, 822, 925
TatI WGTACW 1 cut(s) 135
TauI GCSGC 3 cut(s) 130, 222, 242
Tru1I TTAA 5 cut(s) 450, 540, 723, 740, 758
Tru9I TTAA 5 cut(s) 450, 540, 723, 740, 758
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 2 cut(s) 97, 305
TseI GCWGC 2 cut(s) 25, 318
Tsp45I GTSAC 2 cut(s) 97, 305
TspDTI ATGAA 3 cut(s) 585, 803, 815
TspRI CASTG 1 cut(s) 211
Tth111I GACNNNGTC 1 cut(s) 263
Van91I CCANNNNNTGG 1 cut(s) 646
VpaK11BI GGWCC 1 cut(s) 82
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 1 cut(s) 525
XceI RCATGY 1 cut(s) 416
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 2 cut(s) 780, 897
ZraI GACGTC 1 cut(s) 262
Zsp2I ATGCAT 2 cut(s) 414, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.