MD17G1143700.v1.1

Mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0028781
Physical Location & Seq
Forward (+)
12945614 .. 12947258
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1143700.v1.1.491

Sequence Viewer

Length: 471 bp
ATGCACGATCTTCCGCCGGTGGAGGTAGGCGAGGCCGCTGAGGATTTGCCTGGCCAAATCGGAGAGGGCCCTCTCGTTCCCGATGTGCTGGCCCTCCAGCGACCCGCCGTCCATGTACTCGAGCAGGACCTGGATCTCGCCGTTGTGGTCGGACAAGTCGTGGCACTTGACGACGTTGGGGTTATCGACGTCGCGCAGGATTTCGATCTCGCGGCAGATCTGGCGGAGGGTTTCGATCTCGTGGTTGCCGTAAATCACCTTGAGCGCGTAAAGACGGCCCGTGGGGCGGTGGATGACCTTGTAGACGGTGCCTCCGGCTCCGCTCCCGATGCGGTTGGAGCGCTCGAGCTCGGAGAAGTTGATAAGCTGCTGGGCCGCGCTGTGGGCGGAGGAGGACTGGGCCGAGCTGGGGCCGGGCGGAGGGAGAGGGAGCGGTACAGCAAGCGATGTGTCCCGCTGAGGGAGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

157

Amino Acids

16.11

Weight (kDa)

4.38

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028781)

Species Orthologous Gene IDs
malus_domestica MD17G1143700.v1.1
pyrus_communis pycom17g13680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 308
AccBSI CCGCTC 2 cut(s) 323, 433
AccI GTMKAC 1 cut(s) 303
AccII CGCG 4 cut(s) 194, 212, 267, 378
AclWI GGATC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 52
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 117, 437
AfeI AGCGCT 1 cut(s) 342
AfiI CCNNNNNNNGG 5 cut(s) 286, 382, 409, 420, 460
AhdI GACNNNNNGTC 1 cut(s) 107
AjnI CCWGG 2 cut(s) 49, 129
AluBI AGCT 3 cut(s) 349, 367, 407
AluI AGCT 3 cut(s) 349, 367, 407
Alw21I GWGCWC 1 cut(s) 351
AlwI GGATC 1 cut(s) 141
AlwNI CAGNNNCTG 1 cut(s) 130
Ama87I CYCGRG 2 cut(s) 119, 344
Aor51HI AGCGCT 1 cut(s) 342
AoxI GGCC 8 cut(s) 33, 52, 67, 90, 276, 373, 400, 411
ApaI GGGCCC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 367
AspLEI GCGC 4 cut(s) 196, 267, 343, 380
AspS9I GGNCC 8 cut(s) 67, 68, 91, 127, 277, 373, 400, 411
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 2 cut(s) 119, 344
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 71
BalI TGGCCA 1 cut(s) 54
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 2 cut(s) 71, 351
BauI CACGAG 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 351
BbvCI CCTCAGC 2 cut(s) 39, 458
BbvI GCAGC 1 cut(s) 354
BceAI ACGGC 4 cut(s) 92, 125, 233, 291
BciT130I CCWGG 2 cut(s) 51, 131
BcnI CCSGG 1 cut(s) 415
BfoI RGCGCY 1 cut(s) 344
BglI GCCNNNNNGGC 1 cut(s) 284
BglII AGATCT 1 cut(s) 217
BisI GCNGC 4 cut(s) 36, 213, 368, 376
BlsI GCNGC 4 cut(s) 37, 214, 369, 377
Bme1390I CCNGG 3 cut(s) 51, 131, 415
Bme18I GGWCC 1 cut(s) 127
BmeRI GACNNNNNGTC 1 cut(s) 107
BmeT110I CYCGRG 2 cut(s) 119, 344
BmgT120I GGNCC 8 cut(s) 67, 68, 91, 127, 277, 373, 400, 411
BmiI GGNNCC 4 cut(s) 69, 310, 319, 412
BmrFI CCNGG 3 cut(s) 51, 131, 415
BmrI ACTGGG 1 cut(s) 407
BmsI GCATC 1 cut(s) 319
BmuI ACTGGG 1 cut(s) 407
BpmI CTGGAG 1 cut(s) 80
Bpu10I CCTNAGC 2 cut(s) 39, 458
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 415
BsaBI GATNNNNATC 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 280
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 5 cut(s) 286, 382, 409, 420, 460
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 2 cut(s) 51, 131
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 298
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 5 cut(s) 286, 382, 409, 420, 460
BseMII CTCAG 2 cut(s) 30, 449
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 405
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 1 cut(s) 354
BseYI CCCAGC 2 cut(s) 370, 407
Bsh1236I CGCG 4 cut(s) 194, 212, 267, 378
BshFI GGCC 8 cut(s) 35, 54, 69, 92, 278, 375, 402, 413
BshNI GGYRCC 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 2 cut(s) 119, 344
BsiSI CCGG 3 cut(s) 17, 315, 414
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 5 cut(s) 286, 382, 409, 420, 460
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 8 cut(s) 35, 54, 69, 92, 278, 375, 402, 413
BsoBI CYCGRG 2 cut(s) 119, 344
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 71, 351
Bsp143I GATC 5 cut(s) 7, 133, 205, 217, 235
BspANI GGCC 8 cut(s) 35, 54, 69, 92, 278, 375, 402, 413
BspCNI CTCAG 2 cut(s) 31, 450
BspFNI CGCG 4 cut(s) 194, 212, 267, 378
BspLI GGNNCC 4 cut(s) 69, 310, 319, 412
BspPI GGATC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 308
BsrBI CCGCTC 2 cut(s) 323, 433
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 5 cut(s) 7, 133, 205, 217, 235
BssNI GRCGYC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 51, 131
Bst4CI ACNGT 1 cut(s) 308
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 90, 443
BstDEI CTNAG 2 cut(s) 39, 458
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 1 cut(s) 298
BstFNI CGCG 4 cut(s) 194, 212, 267, 378
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 4 cut(s) 196, 267, 343, 380
BstKTI GATC 5 cut(s) 10, 136, 208, 220, 238
BstMBI GATC 5 cut(s) 7, 133, 205, 217, 235
BstMWI GCNNNNNNNGC 5 cut(s) 221, 284, 329, 338, 384
BstNI CCWGG 2 cut(s) 51, 131
BstSCI CCNGG 3 cut(s) 49, 129, 413
BstSLI GKGCMC 1 cut(s) 71
BstUI CGCG 4 cut(s) 194, 212, 267, 378
BstV1I GCAGC 1 cut(s) 354
BstX2I RGATCY 2 cut(s) 133, 217
BstYI RGATCY 2 cut(s) 133, 217
BsuRI GGCC 8 cut(s) 35, 54, 69, 92, 278, 375, 402, 413
BtgI CCRYGG 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 460
BtsCI GGATG 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 90, 443
CaiI CAGNNNCTG 1 cut(s) 130
CfoI GCGC 4 cut(s) 196, 267, 343, 380
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 8 cut(s) 67, 68, 91, 127, 277, 373, 400, 411
Csp6I GTAC 2 cut(s) 116, 436
CviAII CATG 1 cut(s) 113
CviQI GTAC 2 cut(s) 116, 436
DdeI CTNAG 2 cut(s) 39, 458
DpnI GATC 5 cut(s) 9, 135, 207, 219, 237
DpnII GATC 5 cut(s) 7, 133, 205, 217, 235
DriI GACNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 52
Eam1105I GACNNNNNGTC 1 cut(s) 107
EciI GGCGGA 3 cut(s) 239, 402, 433
Ecl136II GAGCTC 1 cut(s) 349
Eco24I GRGCYC 2 cut(s) 71, 351
Eco47I GGWCC 1 cut(s) 127
Eco47III AGCGCT 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 349
Eco88I CYCGRG 2 cut(s) 119, 344
EcoICRI GAGCTC 1 cut(s) 349
EcoO109I RGGNCCY 3 cut(s) 67, 68, 127
EcoRII CCWGG 2 cut(s) 49, 129
EcoT38I GRGCYC 2 cut(s) 71, 351
FaeI CATG 1 cut(s) 116
FaiI YATR 1 cut(s) 114
FaqI GGGAC 1 cut(s) 437
FatI CATG 1 cut(s) 112
FauI CCCGC 2 cut(s) 112, 462
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 4 cut(s) 36, 213, 368, 376
FokI GGATG 1 cut(s) 305
FriOI GRGCYC 2 cut(s) 71, 351
Fsp4HI GCNGC 4 cut(s) 36, 213, 368, 376
GlaI GCGC 4 cut(s) 195, 266, 342, 379
GluI GCNGC 4 cut(s) 36, 213, 368, 376
GsaI CCCAGC 2 cut(s) 374, 411
GsuI CTGGAG 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 344
HaeIII GGCC 8 cut(s) 35, 54, 69, 92, 278, 375, 402, 413
HapII CCGG 3 cut(s) 17, 315, 414
HhaI GCGC 4 cut(s) 196, 267, 343, 380
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 1 cut(s) 116
Hin6I GCGC 4 cut(s) 194, 265, 341, 378
HinP1I GCGC 4 cut(s) 194, 265, 341, 378
HpaII CCGG 3 cut(s) 17, 315, 414
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 304
Hpy188I TCNGA 3 cut(s) 62, 152, 353
Hpy188III TCNNGA 2 cut(s) 80, 326
Hpy8I GTNNAC 1 cut(s) 304
Hpy99I CGWCG 3 cut(s) 176, 191, 194
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 2 cut(s) 174, 189
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 284, 329, 338, 384
HpyF3I CTNAG 2 cut(s) 39, 458
HpySE526I ACGT 2 cut(s) 174, 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 1 cut(s) 116
HspAI GCGC 4 cut(s) 194, 265, 341, 378
Kzo9I GATC 5 cut(s) 7, 133, 205, 217, 235
LmnI GCTCC 4 cut(s) 323, 328, 338, 430
Lsp1109I GCAGC 1 cut(s) 354
LweI GCATC 1 cut(s) 319
MaeII ACGT 2 cut(s) 174, 189
MalI GATC 5 cut(s) 9, 135, 207, 219, 237
MbiI CCGCTC 2 cut(s) 323, 433
MboI GATC 5 cut(s) 7, 133, 205, 217, 235
MflI RGATCY 2 cut(s) 133, 217
MhlI GDGCHC 2 cut(s) 71, 351
MlsI TGGCCA 1 cut(s) 54
MluNI TGGCCA 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 130, 316
Mox20I TGGCCA 1 cut(s) 54
MscI TGGCCA 1 cut(s) 54
Msp20I TGGCCA 1 cut(s) 54
MspA1I CMGCKG 2 cut(s) 38, 457
MspI CCGG 3 cut(s) 17, 315, 414
MspR9I CCNGG 3 cut(s) 51, 131, 415
MvaI CCWGG 2 cut(s) 51, 131
MvnI CGCG 4 cut(s) 194, 212, 267, 378
MwoI GCNNNNNNNGC 5 cut(s) 221, 284, 329, 338, 384
NciI CCSGG 1 cut(s) 415
NdeII GATC 5 cut(s) 7, 133, 205, 217, 235
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 4 cut(s) 69, 310, 319, 412
NmeAIII GCCGAG 1 cut(s) 428
PaeR7I CTCGAG 2 cut(s) 119, 344
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 246
PkrI GCNGC 4 cut(s) 37, 214, 369, 377
PpuMI RGGWCCY 1 cut(s) 127
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 49, 129
PspFI CCCAGC 2 cut(s) 370, 407
PspGI CCWGG 2 cut(s) 49, 129
PspN4I GGNNCC 4 cut(s) 69, 310, 319, 412
PspOMI GGGCCC 1 cut(s) 67
PspPI GGNCC 8 cut(s) 67, 68, 91, 127, 277, 373, 400, 411
PspPPI RGGWCCY 1 cut(s) 127
PspXI VCTCGAGB 2 cut(s) 119, 344
PstNI CAGNNNCTG 1 cut(s) 130
PsuI RGATCY 2 cut(s) 133, 217
RsaI GTAC 2 cut(s) 117, 437
RsaNI GTAC 2 cut(s) 116, 436
SacI GAGCTC 1 cut(s) 351
SatI GCNGC 4 cut(s) 36, 213, 368, 376
Sau3AI GATC 5 cut(s) 7, 133, 205, 217, 235
Sau96I GGNCC 8 cut(s) 67, 68, 91, 127, 277, 373, 400, 411
ScrFI CCNGG 3 cut(s) 51, 131, 415
SduI GDGCHC 2 cut(s) 71, 351
SetI ASST 9 cut(s) 27, 132, 177, 192, 261, 300, 351, 369, 409
SfaNI GCATC 1 cut(s) 319
Sfr274I CTCGAG 2 cut(s) 119, 344
SgrAI CRCCGGYG 1 cut(s) 16
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 2 cut(s) 119, 344
SmlI CTYRAG 3 cut(s) 119, 260, 344
SmoI CTYRAG 3 cut(s) 119, 260, 344
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 3 cut(s) 49, 129, 413
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 2 cut(s) 177, 192
TaqI TCGA 5 cut(s) 120, 186, 204, 234, 345
TatI WGTACW 1 cut(s) 115
TauI GCSGC 3 cut(s) 38, 215, 378
TseI GCWGC 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 127
XhoI CTCGAG 2 cut(s) 119, 344
XmiI GTMKAC 1 cut(s) 303
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.