MD17G1169000.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0028789
Physical Location & Seq
Reverse (-)
17175492 .. 17175819
328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1169000.v1.1.491

Sequence Viewer

Length: 282 bp
ATGGTTGATCTTGTGCAGTTGGTCGAGCTGCTAGGTGGTATATTGGATGACAGCAGAATAGTGATATTCATGGATACAAAGAAGGGATGTGAATCACATGATCAGATCGCCCTGCAGCTTTGCACGGACGGTCAGCATGCAGTCTTGGTTCATGAAAACGAAAGTCAAGCGGAAAGGGTCCGGATTGTCTCTGAGTTTCAAGCTGGCAAGATCCCCATAATGATTGCAACAGATGTAGCCGCTCGTGTTCTAGGTATCAATAGAATTTCTCTTAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05202 ko05205 map03040 map05202 map05205
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

94

Amino Acids

10.2

Weight (kDa)

4.95

Isoelectric Point (pI)

22.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 8 - 90 9.4e-11 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028789)

Species Orthologous Gene IDs
malus_domestica MD17G1169000.v1.1
pyrus_communis pycom11g03160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 242
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 2 cut(s) 170, 240
AclWI GGATC 1 cut(s) 205
AcsI RAATTY 1 cut(s) 264
AgsI TTSAA 1 cut(s) 200
AluBI AGCT 3 cut(s) 28, 118, 203
AluI AGCT 3 cut(s) 28, 118, 203
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 1 cut(s) 205
Aor13HI TCCGGA 1 cut(s) 180
ApeKI GCWGC 2 cut(s) 28, 115
ApoI RAATTY 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 178
AvaII GGWCC 1 cut(s) 178
BauI CACGAG 1 cut(s) 243
BbvI GCAGC 2 cut(s) 15, 127
BciVI GTATCC 1 cut(s) 67
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 32, 251
BfmI CTRYAG 1 cut(s) 113
BfuI GTATCC 1 cut(s) 67
BisI GCNGC 3 cut(s) 29, 116, 240
BlsI GCNGC 3 cut(s) 30, 117, 241
Bme18I GGWCC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 178
BmiI GGNNCC 1 cut(s) 179
BsaBI GATNNNNATC 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 91
BseAI TCCGGA 1 cut(s) 180
BseGI GGATG 2 cut(s) 52, 92
BseJI GATNNNNATC 1 cut(s) 91
BseMII CTCAG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 15, 127
BsgI GTGCAG 1 cut(s) 35
BsiSI CCGG 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 193
Bsp13I TCCGGA 1 cut(s) 180
Bsp143I GATC 4 cut(s) 7, 100, 105, 210
BspACI CCGC 2 cut(s) 170, 240
BspCNI CTCAG 1 cut(s) 184
BspEI TCCGGA 1 cut(s) 180
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 179
BspMAI CTGCAG 1 cut(s) 117
BspPI GGATC 1 cut(s) 205
BsrBI CCGCTC 1 cut(s) 242
BssMI GATC 4 cut(s) 7, 100, 105, 210
BssSI CACGAG 1 cut(s) 243
Bst2BI CACGAG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 138, 205
BstDEI CTNAG 2 cut(s) 192, 272
BstF5I GGATG 2 cut(s) 52, 92
BstKTI GATC 4 cut(s) 10, 103, 108, 213
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 4 cut(s) 7, 100, 105, 210
BstNSI RCATGY 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 15, 127
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BsuI GTATCC 1 cut(s) 67
BtsCI GGATG 2 cut(s) 52, 92
Cac8I GCNNGC 2 cut(s) 138, 205
CciI TCATGA 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 178
CviAII CATG 4 cut(s) 70, 98, 137, 152
CviJI RGCY 4 cut(s) 28, 118, 203, 239
CviKI_1 RGCY 4 cut(s) 28, 118, 203, 239
DdeI CTNAG 2 cut(s) 192, 272
DpnI GATC 4 cut(s) 9, 102, 107, 212
DpnII GATC 4 cut(s) 7, 100, 105, 210
Eco47I GGWCC 1 cut(s) 178
FaeI CATG 4 cut(s) 73, 101, 140, 155
FaiI YATR 6 cut(s) 41, 71, 99, 138, 153, 218
FatI CATG 4 cut(s) 69, 97, 136, 151
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 29, 116, 240
FokI GGATG 2 cut(s) 59, 99
Fsp4HI GCNGC 3 cut(s) 29, 116, 240
FspBI CTAG 2 cut(s) 32, 251
GluI GCNGC 3 cut(s) 29, 116, 240
HapII CCGG 1 cut(s) 181
Hin1II CATG 4 cut(s) 73, 101, 140, 155
HinfI GANTC 1 cut(s) 92
HpaII CCGG 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 105, 193
Hpy188III TCNNGA 2 cut(s) 152, 181
HpyAV CCTTC 1 cut(s) 76
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4V TGCA 5 cut(s) 16, 115, 123, 140, 227
HpyF3I CTNAG 2 cut(s) 192, 272
Hsp92II CATG 4 cut(s) 73, 101, 140, 155
Kpn2I TCCGGA 1 cut(s) 180
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 4 cut(s) 7, 100, 105, 210
LpnPI CCDG 3 cut(s) 125, 189, 194
Lsp1109I GCAGC 2 cut(s) 15, 127
MaeI CTAG 2 cut(s) 32, 251
MalI GATC 4 cut(s) 9, 102, 107, 212
MbiI CCGCTC 1 cut(s) 242
MboI GATC 4 cut(s) 7, 100, 105, 210
MflI RGATCY 1 cut(s) 210
MluCI AATT 1 cut(s) 264
MroI TCCGGA 1 cut(s) 180
MspI CCGG 1 cut(s) 181
NdeII GATC 4 cut(s) 7, 100, 105, 210
NlaIII CATG 4 cut(s) 73, 101, 140, 155
NlaIV GGNNCC 1 cut(s) 179
NspI RCATGY 1 cut(s) 140
PaeI GCATGC 1 cut(s) 140
PagI TCATGA 1 cut(s) 151
PfeI GAWTC 1 cut(s) 92
PkrI GCNGC 3 cut(s) 30, 117, 241
PspN4I GGNNCC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 178
PstI CTGCAG 1 cut(s) 117
PsuI RGATCY 1 cut(s) 210
SatI GCNGC 3 cut(s) 29, 116, 240
Sau3AI GATC 4 cut(s) 7, 100, 105, 210
Sau96I GGNCC 1 cut(s) 178
SetI ASST 5 cut(s) 30, 37, 120, 205, 256
SfcI CTRYAG 1 cut(s) 113
SinI GGWCC 1 cut(s) 178
SphI GCATGC 1 cut(s) 140
Sse9I AATT 1 cut(s) 264
SsiI CCGC 2 cut(s) 170, 240
SspMI CTAG 2 cut(s) 32, 251
TaaI ACNGT 1 cut(s) 131
TaqI TCGA 1 cut(s) 24
TasI AATT 1 cut(s) 264
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 1 cut(s) 92
TseI GCWGC 2 cut(s) 28, 115
TspDTI ATGAA 3 cut(s) 58, 140, 168
TspGWI ACGGA 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 178
XapI RAATTY 1 cut(s) 264
XceI RCATGY 1 cut(s) 140
XspI CTAG 2 cut(s) 32, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.