MD17G1257200.v1.1

Pentatricopeptide repeat-containing protein At1g77360, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0028792
Physical Location & Seq
Forward (+)
31807644 .. 31808279
636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1257200.v1.1.491

Sequence Viewer

Length: 636 bp
ATGAGGTGTTATCCGGGGATCAATTTTTTCAGGCTTGCGCTCGATGAGTGTGTCAATAAGGGCGATGCTAGGGGAGCTGACAATTTTTGGCGAGCAATGATAGGGAGAATTGGATTCCGACCTGATACAAGTATGTACAATGCGGTGATTTCTTTACATTGTCATGATGGCAATGTAGATTTAGCGAAAAGGATATTGGACGAGATGGTGTACGATGGGCCGTTCCCGAATTCACAAATATATAACACGGTGTTTAAGTTTTTAATCAAGGATAGGAAGTTAAAGGAAGCTTCGACATTGTTTACTGAGATGGTTAAAAATGAGTGTGTTATAGAGCTCGTTAATTGTGACACAACAGTAAGGATTTTCCTGGATTCTGGGGACCTGTACTTTGCTATAAAAGTTTGGAAGTGCATGATGGAGAATTACCATTCTGGCTTGGAGGATACCGGGAATTTGTTGGTCATAGGGCTATGTGATCTGAATAGGGTACCGGAAGCAGTCAAGTACGCGGATGATATGATTGCAAGAGGAATCAAGTTAGAGTTTTCAACTTTGTCAAAGCTGAAACAGAACCTCTTCCAAGCAAGGAAGGAAAACGTGTACGATGAACTTTTGAGGAAATGGAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

212

Amino Acids

24.35

Weight (kDa)

6.82

Isoelectric Point (pI)

24.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 38 - 70 2.1e-07 PPR repeat
PPR_2 PF13041 41 - 78 8.3e-07 PPR repeat family
PPR PF01535 45 - 73 7.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028792)

Species Orthologous Gene IDs
malus_domestica MD17G1257200.v1.1
rosa_chinensis RchiOBHm_Chr3g0493421

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccII CGCG 1 cut(s) 512
AciI CCGC 2 cut(s) 143, 512
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 2 cut(s) 229, 454
AfaI GTAC 6 cut(s) 137, 212, 389, 492, 509, 605
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 1 cut(s) 552
AjnI CCWGG 1 cut(s) 369
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 77, 290, 337, 565, 632
AluI AGCT 5 cut(s) 77, 290, 337, 565, 632
Alw21I GWGCWC 1 cut(s) 339
AlwI GGATC 1 cut(s) 26
AoxI GGCC 1 cut(s) 218
ApoI RAATTY 2 cut(s) 229, 454
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
Asp700I GAANNNNTTC 1 cut(s) 578
Asp718I GGTACC 1 cut(s) 490
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 218, 382
AsuC2I CCSGG 2 cut(s) 15, 451
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 382
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BccI CCATC 5 cut(s) 161, 199, 209, 304, 412
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 1 cut(s) 371
BciVI GTATCC 1 cut(s) 439
BcnI CCSGG 2 cut(s) 15, 451
BfaI CTAG 1 cut(s) 69
BfuI GTATCC 1 cut(s) 439
Bme1390I CCNGG 3 cut(s) 15, 371, 451
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 218, 382
BmiI GGNNCC 2 cut(s) 383, 492
BmrFI CCNGG 3 cut(s) 15, 371, 451
BmsI GCATC 1 cut(s) 55
BpuMI CCSGG 2 cut(s) 15, 451
BsaJI CCNNGG 1 cut(s) 14
BsaWI WCCGGW 1 cut(s) 493
Bse3DI GCAATG 2 cut(s) 102, 178
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 1 cut(s) 14
BseGI GGATG 1 cut(s) 520
BseMI GCAATG 2 cut(s) 102, 178
BseMII CTCAG 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 1 cut(s) 220
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 3 cut(s) 14, 450, 494
BslFI GGGAC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 1 cut(s) 220
Bsp1286I GDGCHC 1 cut(s) 339
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 2 cut(s) 18, 478
BspACI CCGC 2 cut(s) 143, 512
BspANI GGCC 1 cut(s) 220
BspCNI CTCAG 1 cut(s) 298
BspFNI CGCG 1 cut(s) 512
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 383, 492
BspPI GGATC 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 490
BsrDI GCAATG 2 cut(s) 102, 178
BsrGI TGTACA 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 2 cut(s) 18, 478
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 250, 358
Bst6I CTCTTC 1 cut(s) 584
BstAUI TGTACA 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 36, 93
BstDEI CTNAG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 1 cut(s) 512
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 21, 481
BstMBI GATC 2 cut(s) 18, 478
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 3 cut(s) 13, 369, 449
BstUI CGCG 1 cut(s) 512
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 78
BtsCI GGATG 1 cut(s) 520
Cac8I GCNNGC 2 cut(s) 36, 93
CciI TCATGA 1 cut(s) 163
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 218, 382
Csp6I GTAC 6 cut(s) 136, 211, 388, 491, 508, 604
CviAII CATG 2 cut(s) 164, 415
CviJI RGCY 9 cut(s) 34, 77, 220, 290, 337, 438, 472, 565, 632
CviKI_1 RGCY 9 cut(s) 34, 77, 220, 290, 337, 438, 472, 565, 632
CviQI GTAC 6 cut(s) 136, 211, 388, 491, 508, 604
DdeI CTNAG 1 cut(s) 306
DpnI GATC 2 cut(s) 20, 480
DpnII GATC 2 cut(s) 18, 478
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 339
FaeI CATG 2 cut(s) 167, 418
FaqI GGGAC 1 cut(s) 395
FatI CATG 2 cut(s) 163, 414
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 339
FspBI CTAG 1 cut(s) 69
GlaI GCGC 1 cut(s) 39
HaeIII GGCC 1 cut(s) 220
HapII CCGG 3 cut(s) 14, 450, 494
HhaI GCGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 167, 418
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 2 cut(s) 288, 630
HinfI GANTC 3 cut(s) 114, 374, 534
HpaII CCGG 3 cut(s) 14, 450, 494
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 3 cut(s) 211, 303, 604
Hpy188I TCNGA 2 cut(s) 119, 483
Hpy188III TCNNGA 2 cut(s) 164, 226
Hpy8I GTNNAC 3 cut(s) 211, 303, 604
HpyAV CCTTC 1 cut(s) 586
HpyCH4III ACNGT 2 cut(s) 250, 358
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 2 cut(s) 414, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 1 cut(s) 306
HpySE526I ACGT 1 cut(s) 600
Hsp92II CATG 2 cut(s) 167, 418
HspAI GCGC 1 cut(s) 38
KpnI GGTACC 1 cut(s) 494
Kzo9I GATC 2 cut(s) 18, 478
LmnI GCTCC 1 cut(s) 74
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 1 cut(s) 347
MalI GATC 2 cut(s) 20, 480
MboI GATC 2 cut(s) 18, 478
MboII GAAGA 1 cut(s) 571
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 7 cut(s) 22, 82, 108, 229, 343, 424, 454
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 4 cut(s) 436, 524, 587, 612
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 6 cut(s) 255, 263, 281, 315, 342, 634
MslI CAYNNNNRTG 1 cut(s) 162
MspI CCGG 3 cut(s) 14, 450, 494
MspR9I CCNGG 3 cut(s) 15, 371, 451
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 2 cut(s) 15, 451
NdeII GATC 2 cut(s) 18, 478
NlaIII CATG 2 cut(s) 167, 418
NlaIV GGNNCC 2 cut(s) 383, 492
NmuCI GTSAC 1 cut(s) 347
PagI TCATGA 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 3 cut(s) 114, 374, 534
PfoI TCCNGGA 1 cut(s) 369
PpuMI RGGWCCY 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 383, 492
PspPI GGNCC 2 cut(s) 218, 382
PspPPI RGGWCCY 1 cut(s) 382
RsaI GTAC 6 cut(s) 137, 212, 389, 492, 509, 605
RsaNI GTAC 6 cut(s) 136, 211, 388, 491, 508, 604
RseI CAYNNNNRTG 1 cut(s) 162
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 6 cut(s) 255, 263, 281, 315, 342, 634
Sau3AI GATC 2 cut(s) 18, 478
Sau96I GGNCC 2 cut(s) 218, 382
ScrFI CCNGG 3 cut(s) 15, 371, 451
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 1 cut(s) 55
SinI GGWCC 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 162
Sse9I AATT 7 cut(s) 22, 82, 108, 229, 343, 424, 454
SsiI CCGC 2 cut(s) 143, 512
SspMI CTAG 1 cut(s) 69
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 3 cut(s) 13, 369, 449
TaaI ACNGT 2 cut(s) 250, 358
TaiI ACGT 1 cut(s) 603
TaqI TCGA 2 cut(s) 42, 293
TasI AATT 7 cut(s) 22, 82, 108, 229, 343, 424, 454
TatI WGTACW 2 cut(s) 135, 387
TfiI GAWTC 3 cut(s) 114, 374, 534
Tru1I TTAA 6 cut(s) 255, 263, 281, 315, 342, 634
Tru9I TTAA 6 cut(s) 255, 263, 281, 315, 342, 634
TseFI GTSAC 1 cut(s) 347
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 1 cut(s) 624
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 2 cut(s) 229, 454
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.