Prupe.1G081200_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0029176
Physical Location & Seq
Reverse (-)
5944184 .. 5947018
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G081200.1

Sequence Viewer

Length: 1086 bp
ATGGCACGTGAGATTGTGAAGCCCTTGTGGACGTTGTTTTCTGCAATTTTTTTAATTTATTTGGTTAATGGGGCAGCACAACCACAAGTTCCTTGTTACTTCATATTTGGAGACTCATTATCCGACAGTGGAAACAACAATGGCCTCTTCACTGTGGCCAAAGCCAATTTTCGACCCTATGGCATTGATTTTCCAAGGGGTCCAACTGGAAGGTTTTCCAATGGACGTAATTTGGTCGATGCGGTTGCTGAACTACTAGGTTTGGGTAGTTACATTCCACCTTTTGCAACTGCAAGAGGAAAGGAAATACTCAAGGGCGTCAATTATGCATCTGGCTCAGCTGGTATCCGCAACGAAACTGGAAGACAAGTGGGACAGATAATAAGTATGGCTATGCAATTGCAAAATCACCAAAGCATAGTGAAACAGATAGCTTCTTTTAGGGGAAATAATTACTCATCAGCTGAGGAGCACTTAGGCAAATGCATATATTCAGTTGGAATCGGCACCAATGATTACTTTATCAACTACTTTGTTCCCCTGTCCAGCACTAGCCGCCAATATACACCACAGCAATATGCAAGAGTACTCATTGAACAATATTCTCAGCAATTAAGAACGTTGTACAACTTTGGGGCAAGAAAGATAGTTCTCTTCGGGCTAGGTGCTATAGGCTCCGCACCTTCTGAAGTGGCAGCGTGTGGCACAAAAGGCTCTTCTTGCGTTGCTTATATCAACAGTGCAGTTCAAATTTTCAACGGGAGGCTTAAATCACTTGTCCAAGAACTCAACAGCAATCTAAGAAATGCCAAGTTTATTTACATAGACTATTATGGGATTGGATCATCATATGCTTTATCTCGGGGTTCTCTGATTACTAACGTCTCATGTTGTGGAGTGAAAGACGGTTTGAACACATGCATTCCTTTTCAAATCCCATGCAGGAACAGGACCCGGTTCATGTTTTGGGATGGTATACATCCTACAGAGGCAACAAATGTTTTTATAGCAGCAAGAGCATACAAAGCTGAGTTTCTTACAGATGCTTTTCCCTACGATATCCACAGCTTAGCTCAACTATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

39.63

Weight (kDa)

9.25

Isoelectric Point (pI)

27.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029176)

Species Orthologous Gene IDs
prunus_persica Prupe.1G081200_v2.0.a1 Prupe.1G081200_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 976
AciI CCGC 4 cut(s) 242, 349, 556, 678
AclI AACGTT 1 cut(s) 620
AclWI GGATC 1 cut(s) 850
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 1 cut(s) 750
AcuI CTGAAG 1 cut(s) 708
AcvI CACGTG 1 cut(s) 8
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 2 cut(s) 588, 626
AgsI TTSAA 5 cut(s) 596, 749, 757, 913, 932
AluBI AGCT 6 cut(s) 341, 434, 464, 1028, 1068, 1073
AluI AGCT 6 cut(s) 341, 434, 464, 1028, 1068, 1073
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 2 cut(s) 105, 889
AlwI GGATC 1 cut(s) 850
Ama87I CYCGRG 1 cut(s) 861
AoxI GGCC 2 cut(s) 142, 156
ApeKI GCWGC 3 cut(s) 74, 695, 1010
ApoI RAATTY 1 cut(s) 750
Asp700I GAANNNNTTC 1 cut(s) 214
AspS9I GGNCC 2 cut(s) 200, 951
AsuC2I CCSGG 1 cut(s) 955
AsuHPI GGTGA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 2 cut(s) 200, 951
BalI TGGCCA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 506
BbrPI CACGTG 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 3 cut(s) 86, 707, 1022
BccI CCATC 1 cut(s) 965
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciVI GTATCC 1 cut(s) 356
BcnI CCSGG 1 cut(s) 955
BcoDI GTCTC 2 cut(s) 105, 889
BfaI CTAG 3 cut(s) 257, 552, 662
BfmI CTRYAG 2 cut(s) 669, 984
BfuI GTATCC 1 cut(s) 356
BisI GCNGC 4 cut(s) 75, 556, 696, 1011
BlpI GCTNAGC 2 cut(s) 337, 1069
BlsI GCNGC 4 cut(s) 76, 557, 697, 1012
BmcAI AGTACT 1 cut(s) 588
Bme1390I CCNGG 1 cut(s) 955
Bme18I GGWCC 2 cut(s) 200, 951
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 2 cut(s) 200, 951
BmiI GGNNCC 4 cut(s) 201, 508, 676, 953
BmrFI CCNGG 1 cut(s) 955
BmsI GCATC 3 cut(s) 229, 338, 1033
BpiI GAAGAC 1 cut(s) 370
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 2 cut(s) 337, 1069
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 955
BsaAI YACGTR 1 cut(s) 8
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 211, 364
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 2 cut(s) 976, 979
BseMII CTCAG 4 cut(s) 351, 456, 620, 1020
BseNI ACTGG 2 cut(s) 211, 364
BseRI GAGGAG 1 cut(s) 482
BseXI GCAGC 3 cut(s) 86, 707, 1022
BsgI GTGCAG 1 cut(s) 762
BshFI GGCC 2 cut(s) 144, 158
BshNI GGYRCC 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 861
BsiSI CCGG 1 cut(s) 955
BslFI GGGAC 1 cut(s) 387
BsmAI GTCTC 2 cut(s) 105, 889
BsmBI CGTCTC 1 cut(s) 889
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 2 cut(s) 144, 158
BsoBI CYCGRG 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 474
Bsp1407I TGTACA 1 cut(s) 624
Bsp143I GATC 1 cut(s) 842
Bsp1720I GCTNAGC 2 cut(s) 337, 1069
BspACI CCGC 4 cut(s) 242, 349, 556, 678
BspANI GGCC 2 cut(s) 144, 158
BspCNI CTCAG 4 cut(s) 350, 457, 619, 1021
BspLI GGNNCC 4 cut(s) 201, 508, 676, 953
BspPI GGATC 1 cut(s) 850
BspQI GCTCTTC 1 cut(s) 721
BspT107I GGYRCC 1 cut(s) 506
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 2 cut(s) 211, 364
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 1 cut(s) 842
BssNAI GTATAC 1 cut(s) 977
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 194
Bst1107I GTATAC 1 cut(s) 977
Bst4CI ACNGT 4 cut(s) 128, 154, 740, 908
Bst6I CTCTTC 3 cut(s) 152, 659, 721
BstACI GRCGYC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 624
BstBAI YACGTR 1 cut(s) 8
BstDEI CTNAG 7 cut(s) 337, 465, 475, 606, 800, 1029, 1069
BstF5I GGATG 2 cut(s) 976, 979
BstKTI GATC 1 cut(s) 845
BstMAI GTCTC 2 cut(s) 105, 889
BstMBI GATC 1 cut(s) 842
BstMWI GCNNNNNNNGC 5 cut(s) 555, 711, 720, 1016, 1025
BstNSI RCATGY 1 cut(s) 921
BstSCI CCNGG 1 cut(s) 953
BstSFI CTRYAG 2 cut(s) 669, 984
BstV1I GCAGC 3 cut(s) 86, 707, 1022
BstV2I GAAGAC 1 cut(s) 370
BstZ17I GTATAC 1 cut(s) 977
BsuI GTATCC 1 cut(s) 356
BsuRI GGCC 2 cut(s) 144, 158
BtsCI GGATG 2 cut(s) 976, 979
BtsIMutI CAGTG 3 cut(s) 133, 150, 745
Cfr13I GGNCC 2 cut(s) 200, 951
CseI GACGC 1 cut(s) 307
Csp6I GTAC 2 cut(s) 587, 625
CviAII CATG 4 cut(s) 888, 918, 939, 961
CviQI GTAC 2 cut(s) 587, 625
DdeI CTNAG 7 cut(s) 337, 465, 475, 606, 800, 1029, 1069
DpnI GATC 1 cut(s) 844
DpnII GATC 1 cut(s) 842
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 3 cut(s) 152, 659, 721
EarI CTCTTC 3 cut(s) 152, 659, 721
Eco130I CCWWGG 1 cut(s) 194
Eco32I GATATC 1 cut(s) 1060
Eco47I GGWCC 2 cut(s) 200, 951
Eco57I CTGAAG 1 cut(s) 708
Eco72I CACGTG 1 cut(s) 8
Eco88I CYCGRG 1 cut(s) 861
EcoO109I RGGNCCY 1 cut(s) 951
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 1 cut(s) 194
EcoT22I ATGCAT 3 cut(s) 331, 488, 923
ErhI CCWWGG 1 cut(s) 194
Esp3I CGTCTC 1 cut(s) 889
FaeI CATG 4 cut(s) 891, 921, 942, 964
FaqI GGGAC 1 cut(s) 387
FatI CATG 4 cut(s) 887, 917, 938, 960
FauNDI CATATG 1 cut(s) 850
FblI GTMKAC 1 cut(s) 976
Fnu4HI GCNGC 4 cut(s) 75, 556, 696, 1011
FokI GGATG 2 cut(s) 966, 983
Fsp4HI GCNGC 4 cut(s) 75, 556, 696, 1011
FspBI CTAG 3 cut(s) 257, 552, 662
GluI GCNGC 4 cut(s) 75, 556, 696, 1011
HaeIII GGCC 2 cut(s) 144, 158
HapII CCGG 1 cut(s) 955
HgaI GACGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 4 cut(s) 891, 921, 942, 964
HinfI GANTC 2 cut(s) 113, 501
HpaII CCGG 1 cut(s) 955
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 2 cut(s) 30, 977
Hpy188I TCNGA 3 cut(s) 124, 688, 873
Hpy8I GTNNAC 2 cut(s) 30, 977
HpyAV CCTTC 2 cut(s) 204, 693
HpyCH4III ACNGT 4 cut(s) 128, 154, 740, 908
HpyCH4IV ACGT 5 cut(s) 7, 32, 226, 620, 882
HpyF10VI GCNNNNNNNGC 5 cut(s) 555, 711, 720, 1016, 1025
HpyF3I CTNAG 7 cut(s) 337, 465, 475, 606, 800, 1029, 1069
HpySE526I ACGT 5 cut(s) 7, 32, 226, 620, 882
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 4 cut(s) 891, 921, 942, 964
Kzo9I GATC 1 cut(s) 842
LguI GCTCTTC 1 cut(s) 721
LmnI GCTCC 2 cut(s) 469, 680
LpnPI CCDG 9 cut(s) 192, 318, 327, 345, 554, 559, 928, 934, 968
Lsp1109I GCAGC 3 cut(s) 86, 707, 1022
LweI GCATC 3 cut(s) 229, 338, 1033
MaeI CTAG 3 cut(s) 257, 552, 662
MaeII ACGT 5 cut(s) 7, 32, 226, 620, 882
MaeIII GTNAC 2 cut(s) 95, 269
MalI GATC 1 cut(s) 844
MboI GATC 1 cut(s) 842
MboII GAAGA 4 cut(s) 139, 375, 646, 708
MfeI CAATTG 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 474
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 9 cut(s) 45, 54, 166, 229, 322, 398, 451, 611, 750
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 3 cut(s) 147, 227, 478
MnlI CCTC 5 cut(s) 155, 290, 460, 756, 982
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 3 cut(s) 331, 488, 923
MroXI GAANNNNTTC 1 cut(s) 214
MscI TGGCCA 1 cut(s) 158
MseI TTAA 4 cut(s) 53, 66, 614, 768
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 2 cut(s) 341, 464
MspI CCGG 1 cut(s) 955
MspR9I CCNGG 1 cut(s) 955
MunI CAATTG 1 cut(s) 398
Mva1269I GAATGC 1 cut(s) 921
MwoI GCNNNNNNNGC 5 cut(s) 555, 711, 720, 1016, 1025
NciI CCSGG 1 cut(s) 955
NdeI CATATG 1 cut(s) 850
NdeII GATC 1 cut(s) 842
NlaIII CATG 4 cut(s) 891, 921, 942, 964
NlaIV GGNNCC 4 cut(s) 201, 508, 676, 953
NsiI ATGCAT 3 cut(s) 331, 488, 923
NspI RCATGY 1 cut(s) 921
PciSI GCTCTTC 1 cut(s) 721
PctI GAATGC 1 cut(s) 921
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 501
PkrI GCNGC 4 cut(s) 76, 557, 697, 1012
PleI GAGTC 1 cut(s) 107
PmaCI CACGTG 1 cut(s) 8
PmlI CACGTG 1 cut(s) 8
PpsI GAGTC 1 cut(s) 107
Ppu21I YACGTR 1 cut(s) 8
PpuMI RGGWCCY 1 cut(s) 951
Psp1406I AACGTT 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 951
PspCI CACGTG 1 cut(s) 8
PspN4I GGNNCC 4 cut(s) 201, 508, 676, 953
PspPI GGNCC 2 cut(s) 200, 951
PspPPI RGGWCCY 1 cut(s) 951
PvuII CAGCTG 2 cut(s) 341, 464
RsaI GTAC 2 cut(s) 588, 626
RsaNI GTAC 2 cut(s) 587, 625
SapI GCTCTTC 1 cut(s) 721
SaqAI TTAA 4 cut(s) 53, 66, 614, 768
SatI GCNGC 4 cut(s) 75, 556, 696, 1011
Sau3AI GATC 1 cut(s) 842
Sau96I GGNCC 2 cut(s) 200, 951
ScaI AGTACT 1 cut(s) 588
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 955
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 3 cut(s) 229, 338, 1033
SfcI CTRYAG 2 cut(s) 669, 984
SinI GGWCC 2 cut(s) 200, 951
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 9 cut(s) 45, 54, 166, 229, 322, 398, 451, 611, 750
SsiI CCGC 4 cut(s) 242, 349, 556, 678
SspI AATATT 1 cut(s) 602
SspMI CTAG 3 cut(s) 257, 552, 662
StyD4I CCNGG 1 cut(s) 953
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 4 cut(s) 128, 154, 740, 908
TaiI ACGT 5 cut(s) 10, 35, 229, 623, 885
TaqI TCGA 2 cut(s) 172, 237
TasI AATT 9 cut(s) 45, 54, 166, 229, 322, 398, 451, 611, 750
TatI WGTACW 2 cut(s) 586, 624
TauI GCSGC 1 cut(s) 558
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 4 cut(s) 53, 66, 614, 768
Tru9I TTAA 4 cut(s) 53, 66, 614, 768
TscAI CASTG 3 cut(s) 133, 157, 745
TseI GCWGC 3 cut(s) 74, 695, 1010
TspDTI ATGAA 2 cut(s) 91, 949
TspRI CASTG 3 cut(s) 133, 157, 745
VpaK11BI GGWCC 2 cut(s) 200, 951
XapI RAATTY 1 cut(s) 750
XceI RCATGY 1 cut(s) 921
XmiI GTMKAC 1 cut(s) 976
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 3 cut(s) 257, 552, 662
ZrmI AGTACT 1 cut(s) 588
Zsp2I ATGCAT 3 cut(s) 331, 488, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.