Prupe.1G273700_v2.0.a1

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0029134
Physical Location & Seq
Forward (+)
27990512 .. 27992266
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G273700.1

Sequence Viewer

Length: 567 bp
ATGGCACTCGCCTTGTTCGTAAAGGAAGAAGGTTGGGTCGATTTGTCAGACACAAATTCTGGCTCTAGGGTGTTTCTCTTTGCAGCAATCTTTTTGATTTACGTGCTACTCTTCGGCCTCAACATGCATTTTCCTGTTGAGTATCTTGTTGAACCTAAGGAAGTCATGAACTTCACACTGTTTTCACTAGAGTTCATTGACCAAGAGGGAATACCTTCCCTTAGTAATATATATGAACCTAGATTTGGAGGACATCAGACACTTGAGGAAAGAGAGCAATCTTTCTTTGCGAAGAATCAAACTCTACATTGTGGATTTATCAAAGGACCCCCTGGATTCTCAAGCACTGGATTTGATATAGATGTGAAGGACAAGGCATACAAGAACAAGTGTAAGATTGCAGTATCTTCTTGCATTTTTGGAAGCTCTGACTTTCTTAGGAGGCCTACAAGCAAAAAGGTTAATGAGCTAGTCTTTATTGAACACTGTGAGTGCAGAGCACTAGTAAAGCAATTCTGGCATAGGGAAGAGCCATCTCCTCCAGGACCTCCACCACTTGTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616

Protein Analysis

189

Amino Acids

21.36

Weight (kDa)

5.75

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029134)

Species Orthologous Gene IDs
prunus_persica Prupe.1G273700_v2.0.a1 Prupe.1G274100_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 55
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 2 cut(s) 152, 482
AhlI ACTAGT 1 cut(s) 502
AjnI CCWGG 2 cut(s) 331, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 228, 260
AluBI AGCT 2 cut(s) 426, 469
AluI AGCT 2 cut(s) 426, 469
Alw21I GWGCWC 1 cut(s) 502
AoxI GGCC 2 cut(s) 115, 443
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 55
Asp700I GAANNNNTTC 1 cut(s) 214
AspS9I GGNCC 2 cut(s) 326, 545
AvaII GGWCC 2 cut(s) 326, 545
AxyI CCTNAGG 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 541
BciT130I CCWGG 2 cut(s) 333, 543
BcuI ACTAGT 1 cut(s) 502
BfaI CTAG 5 cut(s) 66, 188, 240, 470, 503
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 333, 543
Bme18I GGWCC 2 cut(s) 326, 545
BmgT120I GGNCC 2 cut(s) 326, 545
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 2 cut(s) 333, 543
BpmI CTGGAG 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 284, 325
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 331
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 352
Bse21I CCTNAGG 1 cut(s) 156
BseBI CCWGG 2 cut(s) 333, 543
BseDI CCNNGG 1 cut(s) 331
BseLI CCNNNNNNNGG 1 cut(s) 245
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 528
BseXI GCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 514
BshFI GGCC 2 cut(s) 117, 445
BsiHKAI GWGCWC 1 cut(s) 502
BslI CCNNNNNNNGG 1 cut(s) 245
BsnI GGCC 2 cut(s) 117, 445
Bsp1286I GDGCHC 1 cut(s) 502
BspANI GGCC 2 cut(s) 117, 445
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 328
BspQI GCTCTTC 1 cut(s) 522
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 1 cut(s) 331
Bst2UI CCWGG 2 cut(s) 333, 543
Bst4CI ACNGT 2 cut(s) 180, 488
Bst6I CTCTTC 2 cut(s) 116, 522
BstBAI YACGTR 1 cut(s) 103
BstDEI CTNAG 3 cut(s) 156, 221, 437
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstNI CCWGG 2 cut(s) 333, 543
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 331, 541
BstV1I GCAGC 1 cut(s) 95
Bsu36I CCTNAGG 1 cut(s) 156
BsuRI GGCC 2 cut(s) 117, 445
BtsIMutI CAGTG 3 cut(s) 176, 345, 484
CciI TCATGA 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 326, 545
CviAII CATG 2 cut(s) 124, 166
CviJI RGCY 6 cut(s) 63, 117, 426, 445, 469, 532
CviKI_1 RGCY 6 cut(s) 63, 117, 426, 445, 469, 532
DdeI CTNAG 3 cut(s) 156, 221, 437
Eam1104I CTCTTC 2 cut(s) 116, 522
EarI CTCTTC 2 cut(s) 116, 522
Eco147I AGGCCT 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 326, 545
Eco81I CCTNAGG 1 cut(s) 156
EcoO109I RGGNCCY 2 cut(s) 326, 545
EcoRII CCWGG 2 cut(s) 331, 541
EcoT22I ATGCAT 1 cut(s) 129
FaeI CATG 2 cut(s) 127, 169
FaiI YATR 8 cut(s) 125, 167, 230, 232, 234, 359, 379, 522
FatI CATG 2 cut(s) 123, 165
Fnu4HI GCNGC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 5 cut(s) 66, 188, 240, 470, 503
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 2 cut(s) 117, 445
Hin1II CATG 2 cut(s) 127, 169
HinfI GANTC 2 cut(s) 295, 336
Hpy188I TCNGA 3 cut(s) 49, 258, 430
Hpy188III TCNNGA 1 cut(s) 166
HpyAV CCTTC 3 cut(s) 23, 225, 361
HpyCH4III ACNGT 2 cut(s) 180, 488
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 5 cut(s) 83, 127, 401, 414, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
HpyF3I CTNAG 3 cut(s) 156, 221, 437
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 2 cut(s) 127, 169
LguI GCTCTTC 1 cut(s) 522
LpnPI CCDG 8 cut(s) 45, 147, 318, 333, 345, 502, 528, 555
Lsp1109I GCAGC 1 cut(s) 95
MaeI CTAG 5 cut(s) 66, 188, 240, 470, 503
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 559
MboII GAAGA 5 cut(s) 38, 103, 304, 399, 539
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 2 cut(s) 55, 512
MnlI CCTC 7 cut(s) 128, 199, 242, 259, 435, 549, 558
Mph1103I ATGCAT 1 cut(s) 129
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 1 cut(s) 462
MspR9I CCNGG 2 cut(s) 333, 543
MvaI CCWGG 2 cut(s) 333, 543
MwoI GCNNNNNNNGC 1 cut(s) 517
NlaIII CATG 2 cut(s) 127, 169
NlaIV GGNNCC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 129
NspI RCATGY 1 cut(s) 127
PagI TCATGA 1 cut(s) 165
PceI AGGCCT 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 295, 336
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 1 cut(s) 85
Ppu21I YACGTR 1 cut(s) 103
PpuMI RGGWCCY 2 cut(s) 326, 545
Psp5II RGGWCCY 2 cut(s) 326, 545
Psp6I CCWGG 2 cut(s) 331, 541
PspGI CCWGG 2 cut(s) 331, 541
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 326, 545
PspPPI RGGWCCY 2 cut(s) 326, 545
SapI GCTCTTC 1 cut(s) 522
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 1 cut(s) 84
Sau96I GGNCC 2 cut(s) 326, 545
ScrFI CCNGG 2 cut(s) 333, 543
SduI GDGCHC 1 cut(s) 502
SetI ASST 9 cut(s) 34, 105, 157, 217, 241, 428, 462, 471, 550
SinI GGWCC 2 cut(s) 326, 545
SmlI CTYRAG 2 cut(s) 263, 340
SmoI CTYRAG 2 cut(s) 263, 340
SpeI ACTAGT 1 cut(s) 502
Sse9I AATT 2 cut(s) 55, 512
SseBI AGGCCT 1 cut(s) 445
SspMI CTAG 5 cut(s) 66, 188, 240, 470, 503
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 2 cut(s) 331, 541
TaaI ACNGT 2 cut(s) 180, 488
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 39
TasI AATT 2 cut(s) 55, 512
TfiI GAWTC 2 cut(s) 295, 336
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 3 cut(s) 183, 352, 491
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 3 cut(s) 182, 184, 249
TspRI CASTG 3 cut(s) 183, 352, 491
VpaK11BI GGWCC 2 cut(s) 326, 545
XapI RAATTY 1 cut(s) 55
XceI RCATGY 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 5 cut(s) 66, 188, 240, 470, 503
Zsp2I ATGCAT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.