Prupe.1G279600_v2.0.a1

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0029153
Physical Location & Seq
Reverse (-)
28346720 .. 28351009
4290 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G279600.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 711 bp
ATGGCCCTGCGTGTTTCTTCCTTACTTCTCACGGAAGAAAGGAAGCTTCCGTTTCGCGATGTTTGGGTGGATTGGGGGATTTTTTTTCTTCATGATAGTGGTGAAAAAACGAGGGTGTTTTGTATTTTTGAATCCGGGGGTGATTTTGGGGATTTCGGTGAAGGGACCGGACCGAAAAAGAAGGTGGTTGTAATACAAGATACCGTGGATGATTCTGGGGCTCCATCCCATTCTTGGAAGGGGAACACATATGAGGAGCTTCTTGATAGGGTGTTTAACATTCTTCGTGATCGCGAGAGTAACTTGGAGCTGGCTAGAGACAGGCGTAGGATAGTTTTGAGGCCTCCACAAGTACTTCGTGAGGGCACAGACAGAACAGTTTTTGTGAATTTCATAGATGAGTGCAAGATGATGTATAGGGAGCCGAATCATGTCATGACTTTCTTGCTGACTGAACTGGGGAGAAGTGGATCACTTGATGGGCAACAAAGACGGTGGCAGGCGATGGCGGCCAACGGTGGTGGTGGCAGGTGGCCGGCGGCGGTGGCCGGCGGCGGAAGTTTGGCGGCGAGATATGATTGGCTAGATATGGACATGTGGCATAAATGTCTCTTCTCTCTTAAATGCGAGAAGTGTGGTTCTAAGCGATCAGTTGCTCAAATTAAAGCTGATTTTCTGACTCGTGTGGGATCAAGGAATGCAAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

237

Amino Acids

26.81

Weight (kDa)

8.84

Isoelectric Point (pI)

51.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029153)

Species Orthologous Gene IDs
prunus_persica Prupe.1G026300_v2.0.a1 Prupe.1G279600_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 519
Acc36I ACCTGC 1 cut(s) 519
AccII CGCG 2 cut(s) 57, 294
AciI CCGC 6 cut(s) 509, 539, 542, 552, 555, 566
AclWI GGATC 2 cut(s) 478, 697
AcoI YGGCCR 3 cut(s) 510, 533, 546
AcsI RAATTY 1 cut(s) 388
AfaI GTAC 1 cut(s) 354
AfiI CCNNNNNNNGG 1 cut(s) 234
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 1 cut(s) 131
AluBI AGCT 4 cut(s) 46, 259, 310, 668
AluI AGCT 4 cut(s) 46, 259, 310, 668
Alw26I GTCTC 2 cut(s) 312, 614
AlwI GGATC 2 cut(s) 478, 697
AoxI GGCC 5 cut(s) 3, 341, 510, 533, 546
ApoI RAATTY 1 cut(s) 388
AspS9I GGNCC 3 cut(s) 4, 165, 170
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 3 cut(s) 113, 152, 170
AvaII GGWCC 2 cut(s) 165, 170
BaeGI GKGCMC 1 cut(s) 368
BanII GRGCYC 1 cut(s) 223
BauI CACGAG 1 cut(s) 681
BccI CCATC 3 cut(s) 232, 473, 499
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 312, 614
BfaI CTAG 2 cut(s) 315, 584
BfuAI ACCTGC 1 cut(s) 519
BisI GCNGC 4 cut(s) 510, 540, 553, 567
BlsI GCNGC 4 cut(s) 511, 541, 554, 568
BmcAI AGTACT 1 cut(s) 354
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 2 cut(s) 165, 170
BmgT120I GGNCC 3 cut(s) 4, 165, 170
BmiI GGNNCC 3 cut(s) 166, 222, 423
BmrFI CCNGG 1 cut(s) 136
BmrI ACTGGG 1 cut(s) 467
BmuI ACTGGG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 136
BsaJI CCNNGG 2 cut(s) 135, 204
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse118I RCCGGY 2 cut(s) 535, 548
Bse1I ACTGG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 135, 204
BseGI GGATG 2 cut(s) 214, 224
BseLI CCNNNNNNNGG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 269
BseSI GKGCMC 1 cut(s) 368
Bsh1236I CGCG 2 cut(s) 57, 294
BshFI GGCC 5 cut(s) 5, 343, 512, 535, 548
BsiSI CCGG 4 cut(s) 135, 168, 536, 549
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 2 cut(s) 312, 614
BsmFI GGGAC 1 cut(s) 178
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 5 cut(s) 5, 343, 512, 535, 548
Bsp1286I GDGCHC 2 cut(s) 223, 368
Bsp143I GATC 4 cut(s) 289, 470, 647, 689
Bsp68I TCGCGA 2 cut(s) 57, 294
BspACI CCGC 6 cut(s) 509, 539, 542, 552, 555, 566
BspANI GGCC 5 cut(s) 5, 343, 512, 535, 548
BspFNI CGCG 2 cut(s) 57, 294
BspHI TCATGA 2 cut(s) 91, 435
BspLI GGNNCC 3 cut(s) 166, 222, 423
BspMI ACCTGC 1 cut(s) 519
BspPI GGATC 2 cut(s) 478, 697
BsrFI RCCGGY 2 cut(s) 535, 548
BsrI ACTGG 1 cut(s) 462
BssAI RCCGGY 2 cut(s) 535, 548
BssECI CCNNGG 2 cut(s) 135, 204
BssMI GATC 4 cut(s) 289, 470, 647, 689
BssSI CACGAG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 681
Bst4CI ACNGT 4 cut(s) 205, 379, 495, 518
Bst6I CTCTTC 1 cut(s) 617
BstC8I GCNNGC 4 cut(s) 312, 501, 537, 550
BstDEI CTNAG 1 cut(s) 642
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 214, 224
BstFNI CGCG 2 cut(s) 57, 294
BstKTI GATC 4 cut(s) 292, 473, 650, 692
BstMAI GTCTC 2 cut(s) 312, 614
BstMBI GATC 4 cut(s) 289, 470, 647, 689
BstMWI GCNNNNNNNGC 2 cut(s) 509, 545
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 368
BstUI CGCG 2 cut(s) 57, 294
BsuRI GGCC 5 cut(s) 5, 343, 512, 535, 548
BtgI CCRYGG 1 cut(s) 204
BtgZI GCGATG 2 cut(s) 72, 518
BtsCI GGATG 2 cut(s) 214, 224
BtuMI TCGCGA 2 cut(s) 57, 294
BveI ACCTGC 1 cut(s) 519
Cac8I GCNNGC 4 cut(s) 312, 501, 537, 550
CciI TCATGA 2 cut(s) 91, 435
Cfr10I RCCGGY 2 cut(s) 535, 548
Cfr13I GGNCC 3 cut(s) 4, 165, 170
CpoI CGGWCCG 1 cut(s) 170
Csp6I GTAC 1 cut(s) 353
CspCI CAANNNNNGTGG 4 cut(s) 476, 502, 511, 537
CspI CGGWCCG 1 cut(s) 170
CviAII CATG 5 cut(s) 92, 431, 436, 595, 708
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 1 cut(s) 642
DpnI GATC 4 cut(s) 291, 472, 649, 691
DpnII GATC 4 cut(s) 289, 470, 647, 689
EaeI YGGCCR 3 cut(s) 510, 533, 546
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
EciI GGCGGA 1 cut(s) 570
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 165, 170
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 5 cut(s) 95, 434, 439, 598, 711
FaqI GGGAC 1 cut(s) 178
FatI CATG 5 cut(s) 91, 430, 435, 594, 707
FauNDI CATATG 1 cut(s) 250
Fnu4HI GCNGC 4 cut(s) 510, 540, 553, 567
FokI GGATG 2 cut(s) 211, 221
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 4 cut(s) 510, 540, 553, 567
FspBI CTAG 2 cut(s) 315, 584
GluI GCNGC 4 cut(s) 510, 540, 553, 567
HaeIII GGCC 5 cut(s) 5, 343, 512, 535, 548
HapII CCGG 4 cut(s) 135, 168, 536, 549
Hin1II CATG 5 cut(s) 95, 434, 439, 598, 711
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 4 cut(s) 131, 212, 427, 679
HpaII CCGG 4 cut(s) 135, 168, 536, 549
HphI GGTGA 3 cut(s) 113, 152, 170
Hpy188I TCNGA 1 cut(s) 678
Hpy188III TCNNGA 7 cut(s) 56, 92, 263, 287, 293, 359, 436
HpyAV CCTTC 3 cut(s) 155, 175, 232
HpyCH4III ACNGT 4 cut(s) 205, 379, 495, 518
HpyCH4V TGCA 2 cut(s) 405, 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 545
HpyF3I CTNAG 1 cut(s) 642
Hsp92II CATG 5 cut(s) 95, 434, 439, 598, 711
KroI GCCGGC 2 cut(s) 535, 548
KroNI GCCGGC 2 cut(s) 537, 550
Kzo9I GATC 4 cut(s) 289, 470, 647, 689
LmnI GCTCC 4 cut(s) 226, 256, 307, 421
MaeI CTAG 2 cut(s) 315, 584
MaeIII GTNAC 1 cut(s) 299
MalI GATC 4 cut(s) 291, 472, 649, 691
MboI GATC 4 cut(s) 289, 470, 647, 689
MboII GAAGA 5 cut(s) 9, 47, 80, 275, 604
MhlI GDGCHC 2 cut(s) 223, 368
MluCI AATT 2 cut(s) 388, 660
MlyI GAGTC 1 cut(s) 673
MnlI CCTC 5 cut(s) 105, 247, 333, 354, 355
MroNI GCCGGC 2 cut(s) 535, 548
MseI TTAA 3 cut(s) 276, 621, 663
MslI CAYNNNNRTG 1 cut(s) 96
MspI CCGG 4 cut(s) 135, 168, 536, 549
MspR9I CCNGG 1 cut(s) 136
Mva1269I GAATGC 1 cut(s) 703
MvnI CGCG 2 cut(s) 57, 294
MwoI GCNNNNNNNGC 2 cut(s) 509, 545
NaeI GCCGGC 2 cut(s) 537, 550
NciI CCSGG 1 cut(s) 136
NdeI CATATG 1 cut(s) 250
NdeII GATC 4 cut(s) 289, 470, 647, 689
NgoMIV GCCGGC 2 cut(s) 535, 548
NlaIII CATG 5 cut(s) 95, 434, 439, 598, 711
NlaIV GGNNCC 3 cut(s) 166, 222, 423
NruI TCGCGA 2 cut(s) 57, 294
NspI RCATGY 1 cut(s) 598
PagI TCATGA 2 cut(s) 91, 435
PaqCI CACCTGC 1 cut(s) 519
PceI AGGCCT 1 cut(s) 343
PciI ACATGT 1 cut(s) 594
PctI GAATGC 1 cut(s) 703
PdiI GCCGGC 2 cut(s) 537, 550
PfeI GAWTC 3 cut(s) 131, 212, 427
PkrI GCNGC 4 cut(s) 511, 541, 554, 568
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
PscI ACATGT 1 cut(s) 594
PspN4I GGNNCC 3 cut(s) 166, 222, 423
PspPI GGNCC 3 cut(s) 4, 165, 170
RruI TCGCGA 2 cut(s) 57, 294
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 96
Rsr2I CGGWCCG 1 cut(s) 170
RsrII CGGWCCG 1 cut(s) 170
SaqAI TTAA 3 cut(s) 276, 621, 663
SatI GCNGC 4 cut(s) 510, 540, 553, 567
Sau3AI GATC 4 cut(s) 289, 470, 647, 689
Sau96I GGNCC 3 cut(s) 4, 165, 170
ScaI AGTACT 1 cut(s) 354
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 2 cut(s) 223, 368
SetI ASST 6 cut(s) 48, 186, 261, 312, 533, 670
SinI GGWCC 2 cut(s) 165, 170
SmiMI CAYNNNNRTG 1 cut(s) 96
Sse9I AATT 2 cut(s) 388, 660
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 6 cut(s) 509, 539, 542, 552, 555, 566
SspMI CTAG 2 cut(s) 315, 584
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 1 cut(s) 134
TaaI ACNGT 4 cut(s) 205, 379, 495, 518
TaqII GACCGA 1 cut(s) 187
TasI AATT 2 cut(s) 388, 660
TatI WGTACW 1 cut(s) 352
TauI GCSGC 4 cut(s) 512, 542, 555, 569
TfiI GAWTC 3 cut(s) 131, 212, 427
Tru1I TTAA 3 cut(s) 276, 621, 663
Tru9I TTAA 3 cut(s) 276, 621, 663
TspDTI ATGAA 2 cut(s) 80, 382
TspGWI ACGGA 2 cut(s) 39, 47
VpaK11BI GGWCC 2 cut(s) 165, 170
XapI RAATTY 1 cut(s) 388
XceI RCATGY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XspI CTAG 2 cut(s) 315, 584
ZrmI AGTACT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.