Prupe.1G440000_v2.0.a1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0029157
Physical Location & Seq
Reverse (-)
37397984 .. 37400629
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G440000.1

Sequence Viewer

Length: 1200 bp
ATGGAGGTGGAAAGAACAAAGTTAGGAACCCTAAAAGCTCCTACTCATGGAAAGTTCATTACAGTTCTCAGCATTGATGGAGGTGGAATTAGAGGAATTATTCCAGGAATTATAGTTGCCTTCTTAGAGTCCGAGTTACAGAAGCTAGATGGTGAGGAAGCAAGAGTTGCAGATTACTTCGATGTAGTTGCAGGGACCAGCACAGGTGGTCTGTTGACCATCATGCTAACTGCACCCAATGACAACAATCGCCCTATGTATGCTGCCAAAGATATAAAGTCCTTCTATTTTGATAACGGCCCTAAAATTTTTCCTCAAGGGAGTTGGCCACTTGCCAAAATGATCAAAGCAGTGCTTGGACCAAGGTACGATGGCAAGTATCTCCATAATCTCCTTAAGCAAAAACTGGGAAACACAAGATGTCAACAAACATTGACCAACGTTGTAATCCCAACTTTTGACATCAAGCGACTCCAGCCCACCATCTTCTCCAGCTTTGAGGTGAAGAGCAAGCCACACCTCAATGCCTTACTGTCAGATATAGGTATTGGAACTTCCGCTGCCCCAACTTATTTTCCTGCCCACTATTTTGAAACCAAAGACTCTGCAGGAAAGGTGGAAAATTTCAATCTTGTTGATGGTGGTGTAGCTGCTAACAATCCGACTTTAATTGCTATTGGAGAAGTGACAAAAGAGATTCACAGGAAAAACCCAGATTTCAATTCTATAAAAGCAGCTGATCATTATGGTAGACTACTGGTACTATCACTGGGAACAGGTTCTCCTAAAGTTGAAGAGAAGTACACTGCAAATGAAGCATCCAGATGGGGAGTGTTTGGTTGGTTAGTAAGTGACCATTCTACCCCATTAGTTGATGCCTTCACTCAAGCAAGTGCAGATTTAGTAGACTTTCATATGTCTGTGGTTTTCAAAAGCCTGGAATCCGAAAAAAATTACCTTCGAATTCAGGATGATACATTGAAAGGGACAGTGTCCTCTGTTGATAATTCCACGAAGGAGAATCTGGAAAATCTTGCAAAGATTGGAGAAGAGTTGCTGAAGAAACCGGTGTCAAATGTGAATTTAGAGACGGGTAGTTTTGGACCGTCGAAATGTGAGACCAATGAACAGGCTCTTACAAGGTTTGCAAAGCTGCTCTCTCAAGAGAAGCACCTTCGTGATCAAGCCTCATCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

400

Amino Acids

43.7

Weight (kDa)

6.77

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029157)

Species Orthologous Gene IDs
prunus_persica Prupe.1G440000_v2.0.a1 Prupe.1G440000_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 751, 906
AciI CCGC 1 cut(s) 558
AclI AACGTT 1 cut(s) 441
AcoI YGGCCR 1 cut(s) 326
AcsI RAATTY 4 cut(s) 306, 622, 963, 1081
AcuI CTGAAG 1 cut(s) 1079
AfaI GTAC 3 cut(s) 368, 762, 803
AfiI CCNNNNNNNGG 1 cut(s) 47
AflII CTTAAG 1 cut(s) 395
AgeI ACCGGT 1 cut(s) 1066
AgsI TTSAA 6 cut(s) 593, 628, 721, 794, 931, 982
AjnI CCWGG 2 cut(s) 103, 936
AleI CACNNNNGTG 1 cut(s) 1176
AloI GAACNNNNNNTCC 2 cut(s) 766, 798
AluBI AGCT 6 cut(s) 38, 145, 495, 650, 737, 1153
AluI AGCT 6 cut(s) 38, 145, 495, 650, 737, 1153
Alw26I GTCTC 2 cut(s) 1082, 1112
AoxI GGCC 2 cut(s) 298, 326
ApeKI GCWGC 5 cut(s) 263, 560, 650, 734, 1153
ApoI RAATTY 4 cut(s) 306, 622, 963, 1081
AsiGI ACCGGT 1 cut(s) 1066
Asp700I GAANNNNTTC 1 cut(s) 778
AspS9I GGNCC 4 cut(s) 195, 299, 359, 1103
AsuHPI GGTGA 2 cut(s) 164, 514
AsuII TTCGAA 1 cut(s) 961
AvaII GGWCC 3 cut(s) 195, 359, 1103
BalI TGGCCA 1 cut(s) 328
BarI GAAGNNNNNNTAC 2 cut(s) 538, 570
BbvI GCAGC 5 cut(s) 250, 547, 637, 746, 1140
BccI CCATC 7 cut(s) 71, 143, 227, 365, 491, 632, 819
BceAI ACGGC 1 cut(s) 313
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 2 cut(s) 105, 938
BclI TGATCA 3 cut(s) 342, 739, 1180
BcoDI GTCTC 2 cut(s) 1082, 1112
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 1 cut(s) 606
BfrI CTTAAG 1 cut(s) 395
BisI GCNGC 5 cut(s) 264, 561, 651, 735, 1154
BlsI GCNGC 5 cut(s) 265, 562, 652, 736, 1155
Bme1390I CCNGG 2 cut(s) 105, 938
Bme18I GGWCC 3 cut(s) 195, 359, 1103
BmgT120I GGNCC 4 cut(s) 195, 299, 359, 1103
BmiI GGNNCC 2 cut(s) 28, 196
BmrFI CCNGG 2 cut(s) 105, 938
BmrI ACTGGG 2 cut(s) 416, 779
BmsI GCATC 2 cut(s) 827, 865
BmuI ACTGGG 2 cut(s) 416, 779
BpmI CTGGAG 2 cut(s) 458, 475
Bpu14I TTCGAA 1 cut(s) 961
BpuEI CTTGAG 3 cut(s) 300, 870, 1146
BsaI GGTCTC 1 cut(s) 1112
BsaJI CCNNGG 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 1066
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 3 cut(s) 411, 762, 774
BseBI CCWGG 2 cut(s) 105, 938
BseDI CCNNGG 1 cut(s) 362
BseGI GGATG 2 cut(s) 818, 976
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 3 cut(s) 411, 762, 774
BseXI GCAGC 5 cut(s) 250, 547, 637, 746, 1140
BsgI GTGCAG 2 cut(s) 216, 915
BshFI GGCC 2 cut(s) 300, 328
BshTI ACCGGT 1 cut(s) 1066
BsiSI CCGG 1 cut(s) 1067
BslFI GGGAC 2 cut(s) 208, 1000
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 2 cut(s) 1082, 1112
BsmBI CGTCTC 1 cut(s) 1082
BsmFI GGGAC 2 cut(s) 208, 1000
BsnI GGCC 2 cut(s) 300, 328
Bso31I GGTCTC 1 cut(s) 1112
Bsp119I TTCGAA 1 cut(s) 961
Bsp143I GATC 3 cut(s) 342, 739, 1180
BspACI CCGC 1 cut(s) 558
BspANI GGCC 2 cut(s) 300, 328
BspCNI CTCAG 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 28, 196
BspMAI CTGCAG 1 cut(s) 610
BspQI GCTCTTC 1 cut(s) 500
BspT104I TTCGAA 1 cut(s) 961
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 1112
BsrFI RCCGGY 1 cut(s) 1066
BsrI ACTGG 3 cut(s) 411, 762, 774
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 1 cut(s) 362
BssMI GATC 3 cut(s) 342, 739, 1180
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 2 cut(s) 105, 938
Bst4CI ACNGT 4 cut(s) 64, 534, 991, 1107
Bst6I CTCTTC 3 cut(s) 500, 789, 1044
BstAFI CTTAAG 1 cut(s) 395
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 961
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 3 cut(s) 68, 124, 1197
BstF5I GGATG 2 cut(s) 818, 976
BstKTI GATC 3 cut(s) 345, 742, 1183
BstMAI GTCTC 2 cut(s) 1082, 1112
BstMBI GATC 3 cut(s) 342, 739, 1180
BstMWI GCNNNNNNNGC 3 cut(s) 167, 475, 815
BstNI CCWGG 2 cut(s) 105, 938
BstSCI CCNGG 2 cut(s) 103, 936
BstSFI CTRYAG 1 cut(s) 606
BstV1I GCAGC 5 cut(s) 250, 547, 637, 746, 1140
BsuRI GGCC 2 cut(s) 300, 328
BtsCI GGATG 2 cut(s) 818, 976
BtsI GCAGTG 2 cut(s) 357, 804
BtsIMutI CAGTG 4 cut(s) 357, 767, 804, 996
Cac8I GCNNGC 1 cut(s) 512
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 4 cut(s) 195, 299, 359, 1103
Csp6I GTAC 3 cut(s) 367, 761, 802
CspAI ACCGGT 1 cut(s) 1066
CviAII CATG 2 cut(s) 47, 223
CviQI GTAC 3 cut(s) 367, 761, 802
DdeI CTNAG 3 cut(s) 68, 124, 1197
DpnI GATC 3 cut(s) 344, 741, 1182
DpnII GATC 3 cut(s) 342, 739, 1180
EaeI YGGCCR 1 cut(s) 326
Eam1104I CTCTTC 3 cut(s) 500, 789, 1044
EarI CTCTTC 3 cut(s) 500, 789, 1044
Eco130I CCWWGG 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 1112
Eco47I GGWCC 3 cut(s) 195, 359, 1103
Eco57I CTGAAG 1 cut(s) 1079
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 2 cut(s) 103, 936
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 1082
FaeI CATG 2 cut(s) 50, 226
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 2 cut(s) 208, 1000
FatI CATG 2 cut(s) 46, 222
FauNDI CATATG 1 cut(s) 915
FbaI TGATCA 3 cut(s) 342, 739, 1180
FblI GTMKAC 2 cut(s) 751, 906
Fnu4HI GCNGC 5 cut(s) 264, 561, 651, 735, 1154
FokI GGATG 2 cut(s) 805, 983
Fsp4HI GCNGC 5 cut(s) 264, 561, 651, 735, 1154
FspBI CTAG 1 cut(s) 146
GluI GCNGC 5 cut(s) 264, 561, 651, 735, 1154
GsuI CTGGAG 2 cut(s) 458, 475
HaeIII GGCC 2 cut(s) 300, 328
HapII CCGG 1 cut(s) 1067
Hin1II CATG 2 cut(s) 50, 226
HincII GTYRAC 2 cut(s) 216, 425
HindII GTYRAC 2 cut(s) 216, 425
HinfI GANTC 6 cut(s) 128, 471, 602, 697, 941, 1021
HpaII CCGG 1 cut(s) 1067
HphI GGTGA 2 cut(s) 164, 514
Hpy166II GTNNAC 5 cut(s) 216, 425, 752, 804, 907
Hpy188I TCNGA 4 cut(s) 133, 538, 663, 946
Hpy188III TCNNGA 5 cut(s) 822, 968, 1025, 1163, 1178
Hpy8I GTNNAC 5 cut(s) 216, 425, 752, 804, 907
Hpy99I CGWCG 1 cut(s) 1111
HpyAV CCTTC 6 cut(s) 130, 292, 889, 968, 1009, 1184
HpyCH4III ACNGT 4 cut(s) 64, 534, 991, 1107
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 8 cut(s) 170, 191, 233, 608, 809, 896, 1037, 1148
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 475, 815
HpyF3I CTNAG 3 cut(s) 68, 124, 1197
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 2 cut(s) 50, 226
Ksp22I TGATCA 3 cut(s) 342, 739, 1180
Kzo9I GATC 3 cut(s) 342, 739, 1180
LguI GCTCTTC 1 cut(s) 500
LmnI GCTCC 1 cut(s) 43
Lsp1109I GCAGC 5 cut(s) 250, 547, 637, 746, 1140
LweI GCATC 2 cut(s) 827, 865
MaeI CTAG 1 cut(s) 146
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 3 cut(s) 135, 685, 851
MalI GATC 3 cut(s) 344, 741, 1182
MboI GATC 3 cut(s) 342, 739, 1180
MboII GAAGA 5 cut(s) 478, 517, 806, 1061, 1072
MlsI TGGCCA 1 cut(s) 328
MluNI TGGCCA 1 cut(s) 328
MlyI GAGTC 3 cut(s) 137, 465, 596
MmeI TCCRAC 1 cut(s) 686
MnlI CCTC 8 cut(s) 74, 86, 148, 324, 493, 530, 1006, 1198
Mox20I TGGCCA 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 778
MscI TGGCCA 1 cut(s) 328
MseI TTAA 2 cut(s) 396, 668
MslI CAYNNNNRTG 3 cut(s) 522, 823, 1176
Msp20I TGGCCA 1 cut(s) 328
MspA1I CMGCKG 2 cut(s) 560, 737
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 1 cut(s) 1067
MspR9I CCNGG 2 cut(s) 105, 938
MvaI CCWGG 2 cut(s) 105, 938
MwoI GCNNNNNNNGC 3 cut(s) 167, 475, 815
NdeI CATATG 1 cut(s) 915
NdeII GATC 3 cut(s) 342, 739, 1180
NlaIII CATG 2 cut(s) 50, 226
NlaIV GGNNCC 2 cut(s) 28, 196
NmuCI GTSAC 2 cut(s) 685, 851
NspV TTCGAA 1 cut(s) 961
OliI CACNNNNGTG 1 cut(s) 1176
PciSI GCTCTTC 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 778
PfeI GAWTC 3 cut(s) 697, 941, 1021
PflFI GACNNNGTC 1 cut(s) 991
PfoI TCCNGGA 1 cut(s) 103
PinAI ACCGGT 1 cut(s) 1066
PkrI GCNGC 5 cut(s) 265, 562, 652, 736, 1155
PleI GAGTC 3 cut(s) 136, 465, 596
PpsI GAGTC 3 cut(s) 136, 465, 596
Psp1406I AACGTT 1 cut(s) 441
Psp6I CCWGG 2 cut(s) 103, 936
PspGI CCWGG 2 cut(s) 103, 936
PspN4I GGNNCC 2 cut(s) 28, 196
PspPI GGNCC 4 cut(s) 195, 299, 359, 1103
PstI CTGCAG 1 cut(s) 610
PsyI GACNNNGTC 1 cut(s) 991
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 3 cut(s) 368, 762, 803
RsaNI GTAC 3 cut(s) 367, 761, 802
RseI CAYNNNNRTG 3 cut(s) 522, 823, 1176
SapI GCTCTTC 1 cut(s) 500
SaqAI TTAA 2 cut(s) 396, 668
SatI GCNGC 5 cut(s) 264, 561, 651, 735, 1154
Sau3AI GATC 3 cut(s) 342, 739, 1180
Sau96I GGNCC 4 cut(s) 195, 299, 359, 1103
SchI GAGTC 3 cut(s) 137, 465, 596
ScrFI CCNGG 2 cut(s) 105, 938
SfaNI GCATC 2 cut(s) 827, 865
SfcI CTRYAG 1 cut(s) 606
SfuI TTCGAA 1 cut(s) 961
SinI GGWCC 3 cut(s) 195, 359, 1103
SmiMI CAYNNNNRTG 3 cut(s) 522, 823, 1176
SmlI CTYRAG 4 cut(s) 315, 395, 885, 1161
SmoI CTYRAG 4 cut(s) 315, 395, 885, 1161
SsiI CCGC 1 cut(s) 558
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 103, 936
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 4 cut(s) 64, 534, 991, 1107
TaiI ACGT 1 cut(s) 444
TaqI TCGA 3 cut(s) 180, 961, 1109
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 3 cut(s) 697, 941, 1021
Tru1I TTAA 2 cut(s) 396, 668
Tru9I TTAA 2 cut(s) 396, 668
TscAI CASTG 4 cut(s) 357, 774, 811, 996
TseFI GTSAC 2 cut(s) 685, 851
TseI GCWGC 5 cut(s) 263, 560, 650, 734, 1153
Tsp45I GTSAC 2 cut(s) 685, 851
TspDTI ATGAA 4 cut(s) 46, 828, 902, 1140
TspRI CASTG 4 cut(s) 357, 774, 811, 996
Tth111I GACNNNGTC 1 cut(s) 991
Vha464I CTTAAG 1 cut(s) 395
VpaK11BI GGWCC 3 cut(s) 195, 359, 1103
XapI RAATTY 4 cut(s) 306, 622, 963, 1081
XmiI GTMKAC 2 cut(s) 751, 906
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.