Prupe.1G486300_v2.0.a1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0021140
Physical Location & Seq
Reverse (-)
40493370 .. 40496136
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G486300.1

Sequence Viewer

Length: 1815 bp
ATGAAAACTCAGGGACCCAAGTCACTCCTAGCTCTCATAGAAACCTGCACAAGCCTTAATCAACTGAAGCAAATCCATGCCAAATCTATAATCTCAGGCCTTTCCTACAACCATTTCATCCTCACCCAAATCGCCAATTCCTTCCTGTTCCCTCAGTCTCTGAACTACGCCACACGCATCTTGGTTCAAACCCAAGAGCCAAGCATCTTCATTTACAATTCCATGATCAAAGCATATTCTCAATCAGAAACCCCATTTGTTGCTTTATCAATGTATAACAATATGAGGGTACGCGAAAATGCGGTTGGCGACCAGTTCACGTACCCTTTTGTGTTCAAAGCTTGTGCTAGTGAATTGGCTGTGGAGAAAGGGAGGGAGGTTCATGGTGTGGTTGTTAGAATTGGGTTTGATTCGGATAGGTTTTTGTGTAGTTCTTTGCTAAACTTTTATGGGGTTTGCGGAAAAATTGAGAGTGCAAGGCAGGTGTTTGATGAATTTAAGGCGAAGGATGTTGTTTTCTGGAATGCCATGATAATGGGTTATGCGCGAAATGGGATGGCTTTGGAAGCTTGTGAGGTTTTTAGGGAGATGGTGAAAGTGGGAGAGGTTGAGGCCAATGAAGGCGCGATTTTGGCATTGATATCGGCTTGCACGGTATCGAAAAACTTGAAACTTGGGAGAGAAATTCATGGGTATGTGAGAAAAGAGGTAACTTTTGGGTTAAGTGTGAAACTGGGAGCTGCAATGGTTGACTTGTATGCAAAATGTGGCTGTTTAGATTATGCAAAGAGAGTGTTTGAGGGAATGCCTGAGAAGAATTCTGTGGTGTGGAACTCATTGATAAGTGGTTATTCCCTTAATGGGTTTTCGAGGGAAGCAATTGATCTTTTCAAGGATATGTGTTTTTGGGATGTTAAGCCTGATAGGTACACAATTTCAGGCTTGTTGTCTGCTTGTGCCCAAACCGGAGCTATTAATCTTGGGAATTGGGTTCGGAAATTTGCAGAGAAGAATGGACTTTGGGACGAGTTCATCGGGACCTCTTTGGTAGACCTGTATGCAAAATGCGGTTCTATTGAAGCAGCTAGAGAAGTGTTTGATCAAATGGATAAAAAAACTGTTGCAACATGGAACACTATTCTCTCTGGATATGCGTCAAATGGGCAGGCTGGATCTGCAATAGAGCTCTTTGACGAGATGAGAAAATCAGGTGCTATTCCGGATAGCATAACCTTTCTTTCGATTTTACATGCTTGTGCTCATGCTGGTTTGGTTGAAATAGGCAAGCAATACTTTGATTCGATGGTGAAAAATGATAAGATCACCCCAAAAGTTGAGCATTATGGTTGCATGGTTGATCTTCTTGGCCGTGCTGCACTTCTAAAAGAAGCAAGGGAGCTCATTGAGAGGATGGATATTGAACCAAATGTAATTGTGTGGGGATCACTGTTTAATGCTTGTAGCATTCATGGTGATATTGAGGTGGGAGAGTGGGCAGCTGACCATATTTTCAACTTAGAAGCAATGGATGGGGGTTCCTACGTGTTGTTAGCAAATATGTATGCTGCAGCTGGAAGGTTTGACAGGGTTGAAGCAGTTAGACAGGCAATGGTTGACGCAAGTTTTTGCAAGCTACCTGGATGTAGCATGATTGAGATTGGTGATGTAGTTCATGAGTTTCTTGTTGCTGACAAGACACACCCTAAATCAGAAGAGATCTACTCGGTGTTGGATGAATTAAGCGACAAGCTCAAGACGGCGACAGGCTCCATGCACTTGCCTGCTTTAGCTGGAGAGCATTTGGAAAGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

605

Amino Acids

66.82

Weight (kDa)

5.52

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021140)

Species Orthologous Gene IDs
malus_domestica MD15G1131000.v1.1
prunus_persica Prupe.1G486300_v2.0.a1 Prupe.1G486300_v2.0.a1 Prupe.1G486300_v2.0.a1
pyrus_communis pycom15g11810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 2 cut(s) 53, 472
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 3 cut(s) 294, 547, 626
AccIII TCCGGA 1 cut(s) 1219
AciI CCGC 3 cut(s) 302, 459, 1068
AclWI GGATC 2 cut(s) 1180, 1450
AcoI YGGCCR 1 cut(s) 1366
AcsI RAATTY 4 cut(s) 494, 684, 817, 998
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 3 cut(s) 291, 323, 929
AfiI CCNNNNNNNGG 1 cut(s) 861
AflIII ACRYGT 1 cut(s) 1542
AgsI TTSAA 9 cut(s) 188, 337, 670, 892, 1079, 1277, 1421, 1513, 1592
AjnI CCWGG 1 cut(s) 1636
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
Alw21I GWGCWC 3 cut(s) 1188, 1261, 1401
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 2 cut(s) 1180, 1450
AlwNI CAGNNNCTG 1 cut(s) 160
Aor13HI TCCGGA 1 cut(s) 1219
AoxI GGCC 3 cut(s) 97, 612, 1366
ApeKI GCWGC 6 cut(s) 740, 1082, 1373, 1496, 1565, 1568
ApoI RAATTY 4 cut(s) 494, 684, 817, 998
AseI ATTAAT 1 cut(s) 975
AspLEI GCGC 2 cut(s) 547, 626
AspS9I GGNCC 2 cut(s) 14, 1038
AsuHPI GGTGA 6 cut(s) 115, 604, 1315, 1318, 1484, 1673
AvaII GGWCC 2 cut(s) 14, 1038
BaeGI GKGCMC 1 cut(s) 961
BanII GRGCYC 2 cut(s) 1188, 1401
Bbv12I GWGCWC 3 cut(s) 1188, 1261, 1401
BbvI GCAGC 6 cut(s) 727, 1094, 1360, 1508, 1552, 1580
BccI CCATC 5 cut(s) 550, 583, 1297, 1405, 1523
BceAI ACGGC 2 cut(s) 1353, 1773
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BciT130I CCWGG 1 cut(s) 1638
BclI TGATCA 2 cut(s) 225, 1099
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 3 cut(s) 29, 348, 1086
BfmI CTRYAG 1 cut(s) 1566
BfuAI ACCTGC 2 cut(s) 53, 472
BglII AGATCT 1 cut(s) 1716
BisI GCNGC 6 cut(s) 741, 1083, 1374, 1497, 1566, 1569
BlsI GCNGC 6 cut(s) 742, 1084, 1375, 1498, 1567, 1570
Bme1390I CCNGG 1 cut(s) 1638
Bme18I GGWCC 2 cut(s) 14, 1038
BmgT120I GGNCC 2 cut(s) 14, 1038
BmiI GGNNCC 5 cut(s) 15, 16, 1039, 1537, 1768
BmrFI CCNGG 1 cut(s) 1638
BmrI ACTGGG 1 cut(s) 743
BmsI GCATC 2 cut(s) 186, 213
BmuI ACTGGG 1 cut(s) 743
BoxI GACNNNNGTC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 1706, 1738
BpmI CTGGAG 1 cut(s) 1812
BpuEI CTTGAG 1 cut(s) 1736
BsaAI YACGTR 2 cut(s) 321, 1543
BsaWI WCCGGW 2 cut(s) 965, 1219
Bsc4I CCNNNNNNNGG 1 cut(s) 861
Bse1I ACTGG 2 cut(s) 313, 738
Bse3DI GCAATG 3 cut(s) 750, 1530, 1614
BseAI TCCGGA 1 cut(s) 1219
BseBI CCWGG 1 cut(s) 1638
BseGI GGATG 8 cut(s) 117, 514, 561, 916, 1416, 1534, 1646, 1738
BseLI CCNNNNNNNGG 1 cut(s) 861
BseMI GCAATG 3 cut(s) 750, 1530, 1614
BseMII CTCAG 4 cut(s) 23, 108, 167, 801
BseNI ACTGG 2 cut(s) 313, 738
BseSI GKGCMC 1 cut(s) 961
BseXI GCAGC 6 cut(s) 727, 1094, 1360, 1508, 1552, 1580
BsgI GTGCAG 2 cut(s) 31, 1359
Bsh1236I CGCG 3 cut(s) 294, 547, 626
BshFI GGCC 3 cut(s) 99, 614, 1368
BsiHKAI GWGCWC 3 cut(s) 1188, 1261, 1401
BsiSI CCGG 2 cut(s) 966, 1220
BslFI GGGAC 3 cut(s) 27, 1037, 1051
BslI CCNNNNNNNGG 1 cut(s) 861
BsmAI GTCTC 1 cut(s) 162
BsmFI GGGAC 3 cut(s) 27, 1037, 1051
BsmI GAATGC 3 cut(s) 529, 810, 1464
BsnI GGCC 3 cut(s) 99, 614, 1368
Bsp1286I GDGCHC 4 cut(s) 961, 1188, 1261, 1401
Bsp13I TCCGGA 1 cut(s) 1219
Bsp143I GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
BspACI CCGC 3 cut(s) 302, 459, 1068
BspANI GGCC 3 cut(s) 99, 614, 1368
BspCNI CTCAG 4 cut(s) 22, 107, 166, 802
BspEI TCCGGA 1 cut(s) 1219
BspFNI CGCG 3 cut(s) 294, 547, 626
BspHI TCATGA 1 cut(s) 1672
BspLI GGNNCC 5 cut(s) 15, 16, 1039, 1537, 1768
BspMAI CTGCAG 1 cut(s) 1570
BspMI ACCTGC 2 cut(s) 53, 472
BspPI GGATC 2 cut(s) 1180, 1450
BsrDI GCAATG 3 cut(s) 750, 1530, 1614
BsrI ACTGG 2 cut(s) 313, 738
BssMI GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
Bst2UI CCWGG 1 cut(s) 1638
Bst4CI ACNGT 3 cut(s) 655, 1120, 1449
Bst6I CTCTTC 1 cut(s) 1707
BstBAI YACGTR 2 cut(s) 321, 1543
BstC8I GCNNGC 5 cut(s) 649, 1167, 1286, 1631, 1782
BstDEI CTNAG 5 cut(s) 9, 94, 153, 810, 1516
BstF5I GGATG 8 cut(s) 117, 514, 561, 916, 1416, 1534, 1646, 1738
BstFNI CGCG 3 cut(s) 294, 547, 626
BstHHI GCGC 2 cut(s) 547, 626
BstKTI GATC 8 cut(s) 228, 886, 1102, 1175, 1323, 1360, 1445, 1719
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
BstMWI GCNNNNNNNGC 3 cut(s) 566, 632, 1175
BstNI CCWGG 1 cut(s) 1638
BstNSI RCATGY 1 cut(s) 1253
BstPAI GACNNNNGTC 1 cut(s) 19
BstSCI CCNGG 1 cut(s) 1636
BstSFI CTRYAG 1 cut(s) 1566
BstSLI GKGCMC 1 cut(s) 961
BstUI CGCG 3 cut(s) 294, 547, 626
BstV1I GCAGC 6 cut(s) 727, 1094, 1360, 1508, 1552, 1580
BstX2I RGATCY 2 cut(s) 1172, 1716
BstXI CCANNNNNNTGG 1 cut(s) 535
BstYI RGATCY 2 cut(s) 1172, 1716
BsuRI GGCC 3 cut(s) 99, 614, 1368
BtsCI GGATG 8 cut(s) 117, 514, 561, 916, 1416, 1534, 1646, 1738
BtsIMutI CAGTG 1 cut(s) 1445
BveI ACCTGC 2 cut(s) 53, 472
Cac8I GCNNGC 5 cut(s) 649, 1167, 1286, 1631, 1782
CaiI CAGNNNCTG 1 cut(s) 160
CciI TCATGA 1 cut(s) 1672
CfoI GCGC 2 cut(s) 547, 626
Cfr13I GGNCC 2 cut(s) 14, 1038
CseI GACGC 2 cut(s) 1143, 1625
Csp6I GTAC 3 cut(s) 290, 322, 928
CviQI GTAC 3 cut(s) 290, 322, 928
DdeI CTNAG 5 cut(s) 9, 94, 153, 810, 1516
DpnI GATC 8 cut(s) 227, 885, 1101, 1174, 1322, 1359, 1444, 1718
DpnII GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
EaeI YGGCCR 1 cut(s) 1366
Eam1104I CTCTTC 1 cut(s) 1707
EarI CTCTTC 1 cut(s) 1707
Ecl136II GAGCTC 2 cut(s) 1186, 1399
Eco147I AGGCCT 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 1188, 1401
Eco32I GATATC 1 cut(s) 642
Eco47I GGWCC 2 cut(s) 14, 1038
Eco53kI GAGCTC 2 cut(s) 1186, 1399
Eco57I CTGAAG 1 cut(s) 86
EcoICRI GAGCTC 2 cut(s) 1186, 1399
EcoO109I RGGNCCY 2 cut(s) 14, 1038
EcoRI GAATTC 1 cut(s) 817
EcoRII CCWGG 1 cut(s) 1636
EcoRV GATATC 1 cut(s) 642
EcoT38I GRGCYC 2 cut(s) 1188, 1401
FalI AAGNNNNNCTT 2 cut(s) 1277, 1309
FaqI GGGAC 3 cut(s) 27, 1037, 1051
FbaI TGATCA 2 cut(s) 225, 1099
FblI GTMKAC 1 cut(s) 1050
Fnu4HI GCNGC 6 cut(s) 741, 1083, 1374, 1497, 1566, 1569
FokI GGATG 8 cut(s) 104, 521, 568, 923, 1423, 1541, 1653, 1745
FriOI GRGCYC 2 cut(s) 1188, 1401
Fsp4HI GCNGC 6 cut(s) 741, 1083, 1374, 1497, 1566, 1569
FspBI CTAG 3 cut(s) 29, 348, 1086
GlaI GCGC 2 cut(s) 546, 625
GluI GCNGC 6 cut(s) 741, 1083, 1374, 1497, 1566, 1569
GsuI CTGGAG 1 cut(s) 1812
HaeIII GGCC 3 cut(s) 99, 614, 1368
HapII CCGG 2 cut(s) 966, 1220
HgaI GACGC 2 cut(s) 1143, 1625
HhaI GCGC 2 cut(s) 547, 626
Hin6I GCGC 2 cut(s) 545, 624
HinP1I GCGC 2 cut(s) 545, 624
HincII GTYRAC 2 cut(s) 751, 1615
HindII GTYRAC 2 cut(s) 751, 1615
HindIII AAGCTT 2 cut(s) 339, 567
HinfI GANTC 2 cut(s) 410, 1298
HpaII CCGG 2 cut(s) 966, 1220
HphI GGTGA 6 cut(s) 115, 604, 1315, 1318, 1484, 1673
Hpy166II GTNNAC 5 cut(s) 318, 751, 930, 1051, 1615
Hpy188I TCNGA 5 cut(s) 162, 247, 415, 996, 1711
Hpy188III TCNNGA 6 cut(s) 520, 1036, 1146, 1220, 1673, 1753
Hpy8I GTNNAC 5 cut(s) 318, 751, 930, 1051, 1615
HpyAV CCTTC 4 cut(s) 151, 499, 614, 1569
HpyCH4III ACNGT 3 cut(s) 655, 1120, 1449
HpyCH4IV ACGT 2 cut(s) 320, 1542
HpyF10VI GCNNNNNNNGC 3 cut(s) 566, 632, 1175
HpyF3I CTNAG 5 cut(s) 9, 94, 153, 810, 1516
HpySE526I ACGT 2 cut(s) 320, 1542
HspAI GCGC 2 cut(s) 545, 624
KflI GGGWCCC 1 cut(s) 14
Kpn2I TCCGGA 1 cut(s) 1219
Ksp22I TGATCA 2 cut(s) 225, 1099
Kzo9I GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
LmnI GCTCC 4 cut(s) 737, 968, 1396, 1772
Lsp1109I GCAGC 6 cut(s) 727, 1094, 1360, 1508, 1552, 1580
LweI GCATC 2 cut(s) 186, 213
MaeI CTAG 3 cut(s) 29, 348, 1086
MaeII ACGT 2 cut(s) 320, 1542
MaeIII GTNAC 2 cut(s) 21, 709
MalI GATC 8 cut(s) 227, 885, 1101, 1174, 1322, 1359, 1444, 1718
MboI GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
MboII GAAGA 5 cut(s) 199, 826, 1021, 1352, 1724
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 2 cut(s) 1172, 1716
MhlI GDGCHC 4 cut(s) 961, 1188, 1261, 1401
MmeI TCCRAC 1 cut(s) 1710
MroI TCCGGA 1 cut(s) 1219
MseI TTAA 8 cut(s) 57, 498, 722, 858, 915, 975, 1452, 1739
MslI CAYNNNNRTG 3 cut(s) 533, 693, 1254
MspA1I CMGCKG 2 cut(s) 1499, 1571
MspI CCGG 2 cut(s) 966, 1220
MspR9I CCNGG 1 cut(s) 1638
MunI CAATTG 1 cut(s) 879
Mva1269I GAATGC 3 cut(s) 529, 810, 1464
MvaI CCWGG 1 cut(s) 1638
MvnI CGCG 3 cut(s) 294, 547, 626
MwoI GCNNNNNNNGC 3 cut(s) 566, 632, 1175
NdeII GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
NlaIV GGNNCC 5 cut(s) 15, 16, 1039, 1537, 1768
NmuCI GTSAC 1 cut(s) 21
NspI RCATGY 1 cut(s) 1253
PagI TCATGA 1 cut(s) 1672
PaqCI CACCTGC 1 cut(s) 472
PceI AGGCCT 1 cut(s) 99
PctI GAATGC 3 cut(s) 529, 810, 1464
PfeI GAWTC 2 cut(s) 410, 1298
PkrI GCNGC 6 cut(s) 742, 1084, 1375, 1498, 1567, 1570
Ppu21I YACGTR 2 cut(s) 321, 1543
PpuMI RGGWCCY 2 cut(s) 14, 1038
PshAI GACNNNNGTC 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 975
Psp124BI GAGCTC 2 cut(s) 1188, 1401
Psp5II RGGWCCY 2 cut(s) 14, 1038
Psp6I CCWGG 1 cut(s) 1636
PspGI CCWGG 1 cut(s) 1636
PspN4I GGNNCC 5 cut(s) 15, 16, 1039, 1537, 1768
PspPI GGNCC 2 cut(s) 14, 1038
PspPPI RGGWCCY 2 cut(s) 14, 1038
PstI CTGCAG 1 cut(s) 1570
PstNI CAGNNNCTG 1 cut(s) 160
PsuI RGATCY 2 cut(s) 1172, 1716
PvuII CAGCTG 2 cut(s) 1499, 1571
RsaI GTAC 3 cut(s) 291, 323, 929
RsaNI GTAC 3 cut(s) 290, 322, 928
RseI CAYNNNNRTG 3 cut(s) 533, 693, 1254
SacI GAGCTC 2 cut(s) 1188, 1401
SaqAI TTAA 8 cut(s) 57, 498, 722, 858, 915, 975, 1452, 1739
SatI GCNGC 6 cut(s) 741, 1083, 1374, 1497, 1566, 1569
Sau3AI GATC 8 cut(s) 225, 883, 1099, 1172, 1320, 1357, 1442, 1716
Sau96I GGNCC 2 cut(s) 14, 1038
ScrFI CCNGG 1 cut(s) 1638
SduI GDGCHC 4 cut(s) 961, 1188, 1261, 1401
SfaNI GCATC 2 cut(s) 186, 213
SfcI CTRYAG 1 cut(s) 1566
SinI GGWCC 2 cut(s) 14, 1038
SmiMI CAYNNNNRTG 3 cut(s) 533, 693, 1254
SmlI CTYRAG 1 cut(s) 1751
SmoI CTYRAG 1 cut(s) 1751
SseBI AGGCCT 1 cut(s) 99
SsiI CCGC 3 cut(s) 302, 459, 1068
SspMI CTAG 3 cut(s) 29, 348, 1086
SstI GAGCTC 2 cut(s) 1188, 1401
StuI AGGCCT 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 1636
TaaI ACNGT 3 cut(s) 655, 1120, 1449
TaiI ACGT 2 cut(s) 323, 1545
TaqI TCGA 4 cut(s) 659, 869, 1241, 1301
TfiI GAWTC 2 cut(s) 410, 1298
Tru1I TTAA 8 cut(s) 57, 498, 722, 858, 915, 975, 1452, 1739
Tru9I TTAA 8 cut(s) 57, 498, 722, 858, 915, 975, 1452, 1739
TscAI CASTG 1 cut(s) 1452
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 6 cut(s) 740, 1082, 1373, 1496, 1565, 1568
Tsp45I GTSAC 1 cut(s) 21
TspRI CASTG 1 cut(s) 1452
VpaK11BI GGWCC 2 cut(s) 14, 1038
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 4 cut(s) 494, 684, 817, 998
XceI RCATGY 1 cut(s) 1253
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 1050
XspI CTAG 3 cut(s) 29, 348, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.