Prupe.3G148700_v2.0.a1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0026721
Physical Location & Seq
Reverse (-)
16235943 .. 16237780
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G148700.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 1530 bp
ATGGAGACTTCAGCATCGCCTCCGCCACCAACCACCTCACCCACCAAACCAAAGCTTCGCATTATGTGCAGCTATGGCGGCCACATAGTCCCCAATCCACGCTCCAAGTCCCTCTGCTACATTGGCGGCGACACTCGCATACTCTCAATCGACCCAACCACCGTCTCAACTCTCTCTTCTCTCACATCCCACCTCGCAGCCACTCTCTCTCTCCCCGCACCTCTGTTTCTCAAATACCAACTTCCCCGCCACGACCTCCTCTCCTTAGTCTCTCTCTCCACCGACGACGACCTTCTCATCTTCCTCAACGAGCTTCGCCGCCTCTCCAATTCCGCCTCTCGGATCAGATTGTTTGTGTTTTTCGACAAGACCCATCATCAGTGTAAGGCCATTCAGCACCCAAAGACTGAGTCTTGGTTCATTGATGCGCTGGAGAGTGCTAAGATTGTGATGCAGAAGGGGATGAGGAGTAATGGGTTGGTGGGTTTTGAGGCCGAGAGTCAGAGTCAGAGTGAGTGTTTGAGTGGTGGTGGTAGTAGGAAATTTTTGAGCTGTGGGGTTGGTGGTGGTGGGTCTGTGGCTGCAGAGTCCATTGTGTTGGAGACTGGGTCGTCTTTTGGGTCTACTTCATCTTCAGCTTCTTTGTCTAGTTTGCCCTCTGTTAGAGCTCGAGTTGAGGATGTTATGGCTGGTACATTGCTGGAAAATAGGACTGACGCAGTGATTAATGTTGCTTCAATGGAGAACAGGGTTTCTTATAACTATGCTGAATCAGATATTAAACTTAGTGATCCTAATTTTTCTGGGATCCAGGTCCAGAAAACAGTTCAGGCGTCTGGATATTATCCAGTGACTTCACAACTGGATCAGCTTCAGCAACCGAAGCTACAATTCGTTCAAGTAGGTACCCATTACATACCCCAGAATCCTGTTGGGATGTTGCCAGTTTCATCATACTACCCAATGTACCATCCGCAGCTGCATCAGCAGCAGCTGCAACACTTACACTATCAGCCCAATCAGCCGTACCCGATATACATCTTGCCTGCTGCACCAGCACAACCTTACAATTTGCCAATGCACGGTAGCTCATACCCTATAGCTCATGGTCATCCCCCGTTGTATCCAAATGCTTCCTGTATCCCTCCCCAAGCAACTCAGAACGAACCTATTAAAGCTGGTGTTTCATCTGATTTTGCTTCACGAGTGTACAGACCAGTCCATTCAGAAATGCCACATCATGGAGGAGTGCCACTTATCAATGTTCCATATGATGGAAATCAGCAACAACCATCAGGTGTTTCTGAAATGCAGCATGAAACTGTTGCTATTGCTTCCAGGGAATCTCCTAAATACAGTAATCAGCCTGATAATGACCCTGCACGTGACCTGATATACAAATCTCAGCCTTCAGCACCGTCATTGGCTTACCAAAGCATGACCAAAGATACAATACATTTATCGGAAGCCTTGACTCAGTTTCATATAGACAACCGTAAACAACAGACTGGGAACTTGGACTCACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

510

Amino Acids

55.66

Weight (kDa)

6.7

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026721)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16220
prunus_persica Prupe.3G148700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AasI GACNNNNNNGTC 1 cut(s) 610
Acc65I GGTACC 1 cut(s) 905
AccB1I GGYRCC 1 cut(s) 905
AccB7I CCANNNNNTGG 2 cut(s) 1241, 1274
AccI GTMKAC 1 cut(s) 623
AciI CCGC 8 cut(s) 23, 78, 126, 216, 247, 319, 333, 974
AclWI GGATC 5 cut(s) 350, 785, 802, 815, 873
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 3 cut(s) 618, 857, 1395
AcvI CACGTG 1 cut(s) 1385
AcyI GRCGYC 1 cut(s) 833
AfaI GTAC 5 cut(s) 694, 907, 968, 1028, 1211
AfiI CCNNNNNNNGG 4 cut(s) 339, 1082, 1241, 1274
AgsI TTSAA 2 cut(s) 738, 899
AjnI CCWGG 2 cut(s) 810, 1337
Alw21I GWGCWC 1 cut(s) 670
Alw26I GTCTC 3 cut(s) 169, 274, 596
AlwI GGATC 5 cut(s) 350, 785, 802, 815, 873
AlwNI CAGNNNCTG 1 cut(s) 994
Ama87I CYCGRG 1 cut(s) 669
AoxI GGCC 3 cut(s) 79, 387, 492
ApoI RAATTY 1 cut(s) 542
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
AseI ATTAAT 1 cut(s) 726
Asp718I GGTACC 1 cut(s) 905
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 1 cut(s) 814
AsuHPI GGTGA 1 cut(s) 30
AvaI CYCGRG 1 cut(s) 669
AvaII GGWCC 1 cut(s) 814
BamHI GGATCC 1 cut(s) 807
BanI GGYRCC 1 cut(s) 905
BanII GRGCYC 1 cut(s) 670
BauI CACGAG 1 cut(s) 1203
BbrPI CACGTG 1 cut(s) 1385
Bbv12I GWGCWC 1 cut(s) 670
BccI CCATC 4 cut(s) 381, 978, 1268, 1300
BceAI ACGGC 1 cut(s) 1009
BciT130I CCWGG 2 cut(s) 812, 1339
BciVI GTATCC 2 cut(s) 1134, 1151
BcoDI GTCTC 3 cut(s) 169, 274, 596
BfaI CTAG 1 cut(s) 648
BfmI CTRYAG 2 cut(s) 582, 1098
BfuI GTATCC 2 cut(s) 1134, 1151
Bme1390I CCNGG 2 cut(s) 812, 1339
Bme18I GGWCC 1 cut(s) 814
BmeT110I CYCGRG 1 cut(s) 669
BmgT120I GGNCC 1 cut(s) 814
BmiI GGNNCC 2 cut(s) 809, 907
BmrFI CCNGG 2 cut(s) 812, 1339
BmrI ACTGGG 2 cut(s) 615, 1518
BmsI GCATC 4 cut(s) 23, 415, 441, 991
BmuI ACTGGG 2 cut(s) 615, 1518
BpmI CTGGAG 1 cut(s) 452
BsaAI YACGTR 1 cut(s) 1385
BsaBI GATNNNNATC 2 cut(s) 1037, 1278
BsaHI GRCGYC 1 cut(s) 833
BsaJI CCNNGG 1 cut(s) 1338
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 593, 623
Bsc4I CCNNNNNNNGG 4 cut(s) 339, 1082, 1241, 1274
Bse1I ACTGG 6 cut(s) 610, 848, 867, 944, 1217, 1513
Bse3DI GCAATG 1 cut(s) 695
Bse8I GATNNNNATC 2 cut(s) 1037, 1278
BseBI CCWGG 2 cut(s) 812, 1339
BseDI CCNNGG 1 cut(s) 1338
BseGI GGATG 6 cut(s) 185, 468, 685, 942, 970, 1111
BseJI GATNNNNATC 2 cut(s) 1037, 1278
BseLI CCNNNNNNNGG 4 cut(s) 339, 1082, 1241, 1274
BseMI GCAATG 1 cut(s) 695
BseMII CTCAG 4 cut(s) 399, 1172, 1418, 1490
BseNI ACTGG 6 cut(s) 610, 848, 867, 944, 1217, 1513
BseRI GAGGAG 3 cut(s) 248, 481, 1260
BsgI GTGCAG 3 cut(s) 88, 1035, 1365
BshFI GGCC 3 cut(s) 81, 389, 494
BshNI GGYRCC 1 cut(s) 905
BsiHKAI GWGCWC 1 cut(s) 670
BsiHKCI CYCGRG 1 cut(s) 669
BslFI GGGAC 2 cut(s) 74, 94
BslI CCNNNNNNNGG 4 cut(s) 339, 1082, 1241, 1274
BsmAI GTCTC 3 cut(s) 169, 274, 596
BsmBI CGTCTC 1 cut(s) 169
BsmFI GGGAC 2 cut(s) 74, 94
BsnI GGCC 3 cut(s) 81, 389, 494
BsoBI CYCGRG 1 cut(s) 669
Bsp1286I GDGCHC 1 cut(s) 670
Bsp1407I TGTACA 1 cut(s) 1209
Bsp143I GATC 4 cut(s) 342, 790, 807, 865
BspACI CCGC 8 cut(s) 23, 78, 126, 216, 247, 319, 333, 974
BspANI GGCC 3 cut(s) 81, 389, 494
BspCNI CTCAG 4 cut(s) 400, 1171, 1417, 1489
BspLI GGNNCC 2 cut(s) 809, 907
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 5 cut(s) 350, 785, 802, 815, 873
BspT107I GGYRCC 1 cut(s) 905
BsrDI GCAATG 1 cut(s) 695
BsrGI TGTACA 1 cut(s) 1209
BsrI ACTGG 6 cut(s) 610, 848, 867, 944, 1217, 1513
BssECI CCNNGG 1 cut(s) 1338
BssMI GATC 4 cut(s) 342, 790, 807, 865
BssNI GRCGYC 1 cut(s) 833
BssSI CACGAG 1 cut(s) 1203
Bst2BI CACGAG 1 cut(s) 1203
Bst2UI CCWGG 2 cut(s) 812, 1339
Bst4CI ACNGT 7 cut(s) 163, 826, 1085, 1324, 1358, 1419, 1496
Bst6I CTCTTC 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 833
BstAPI GCANNNNNTGC 2 cut(s) 66, 994
BstAUI TGTACA 1 cut(s) 1209
BstBAI YACGTR 1 cut(s) 1385
BstC8I GCNNGC 1 cut(s) 1047
BstDEI CTNAG 7 cut(s) 265, 408, 441, 785, 1158, 1404, 1476
BstF5I GGATG 6 cut(s) 185, 468, 685, 942, 970, 1111
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 4 cut(s) 345, 793, 810, 868
BstMAI GTCTC 3 cut(s) 169, 274, 596
BstMBI GATC 4 cut(s) 342, 790, 807, 865
BstNI CCWGG 2 cut(s) 812, 1339
BstSCI CCNGG 2 cut(s) 810, 1337
BstSFI CTRYAG 2 cut(s) 582, 1098
BstX2I RGATCY 1 cut(s) 807
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 1 cut(s) 807
BsuI GTATCC 2 cut(s) 1134, 1151
BsuRI GGCC 3 cut(s) 81, 389, 494
BtsCI GGATG 6 cut(s) 185, 468, 685, 942, 970, 1111
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 3 cut(s) 386, 726, 855
Cac8I GCNNGC 1 cut(s) 1047
CaiI CAGNNNCTG 1 cut(s) 994
CfoI GCGC 1 cut(s) 430
Cfr13I GGNCC 1 cut(s) 814
CseI GACGC 2 cut(s) 725, 822
Csp6I GTAC 5 cut(s) 693, 906, 967, 1027, 1210
CviAII CATG 4 cut(s) 1106, 1241, 1316, 1438
CviQI GTAC 5 cut(s) 693, 906, 967, 1027, 1210
DdeI CTNAG 7 cut(s) 265, 408, 441, 785, 1158, 1404, 1476
DpnI GATC 4 cut(s) 344, 792, 809, 867
DpnII GATC 4 cut(s) 342, 790, 807, 865
DrdI GACNNNNNNGTC 1 cut(s) 610
DseDI GACNNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
EciI GGCGGA 2 cut(s) 12, 322
Ecl136II GAGCTC 1 cut(s) 668
Eco24I GRGCYC 1 cut(s) 670
Eco47I GGWCC 1 cut(s) 814
Eco53kI GAGCTC 1 cut(s) 668
Eco57I CTGAAG 3 cut(s) 618, 857, 1395
Eco72I CACGTG 1 cut(s) 1385
Eco88I CYCGRG 1 cut(s) 669
EcoICRI GAGCTC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 810, 1337
EcoT38I GRGCYC 1 cut(s) 670
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 4 cut(s) 1109, 1244, 1319, 1441
FaqI GGGAC 2 cut(s) 74, 94
FatI CATG 4 cut(s) 1105, 1240, 1315, 1437
FauI CCCGC 2 cut(s) 223, 254
FauNDI CATATG 1 cut(s) 1270
FblI GTMKAC 1 cut(s) 623
FokI GGATG 6 cut(s) 172, 475, 692, 949, 957, 1098
FriOI GRGCYC 1 cut(s) 670
FspBI CTAG 1 cut(s) 648
GlaI GCGC 1 cut(s) 429
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 3 cut(s) 81, 389, 494
HgaI GACGC 2 cut(s) 725, 822
HhaI GCGC 1 cut(s) 430
Hin1I GRCGYC 1 cut(s) 833
Hin1II CATG 4 cut(s) 1109, 1244, 1319, 1441
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 9 cut(s) 410, 499, 505, 587, 770, 925, 1343, 1474, 1520
HphI GGTGA 1 cut(s) 30
Hpy166II GTNNAC 3 cut(s) 624, 1210, 1499
Hpy188III TCNNGA 3 cut(s) 817, 837, 1203
Hpy8I GTNNAC 3 cut(s) 624, 1210, 1499
Hpy99I CGWCG 2 cut(s) 287, 290
HpyAV CCTTC 3 cut(s) 302, 451, 1419
HpyCH4III ACNGT 7 cut(s) 163, 826, 1085, 1324, 1358, 1419, 1496
HpyCH4IV ACGT 1 cut(s) 1384
HpyCH4V TGCA 9 cut(s) 69, 454, 584, 982, 997, 1052, 1081, 1312, 1382
HpyF3I CTNAG 7 cut(s) 265, 408, 441, 785, 1158, 1404, 1476
HpySE526I ACGT 1 cut(s) 1384
Hsp92I GRCGYC 1 cut(s) 833
Hsp92II CATG 4 cut(s) 1109, 1244, 1319, 1441
HspAI GCGC 1 cut(s) 428
KpnI GGTACC 1 cut(s) 909
Kzo9I GATC 4 cut(s) 342, 790, 807, 865
LmnI GCTCC 1 cut(s) 107
LweI GCATC 4 cut(s) 23, 415, 441, 991
MaeI CTAG 1 cut(s) 648
MaeII ACGT 1 cut(s) 1384
MaeIII GTNAC 2 cut(s) 850, 1385
MalI GATC 4 cut(s) 344, 792, 809, 867
MboI GATC 4 cut(s) 342, 790, 807, 865
MboII GAAGA 3 cut(s) 168, 292, 624
MflI RGATCY 1 cut(s) 807
MhlI GDGCHC 1 cut(s) 670
MluCI AATT 5 cut(s) 328, 542, 796, 890, 1069
MlyI GAGTC 6 cut(s) 419, 508, 514, 596, 1468, 1514
MmeI TCCRAC 1 cut(s) 579
MseI TTAA 3 cut(s) 726, 780, 1173
MspA1I CMGCKG 2 cut(s) 979, 994
MspR9I CCNGG 2 cut(s) 812, 1339
MvaI CCWGG 2 cut(s) 812, 1339
NdeI CATATG 1 cut(s) 1270
NdeII GATC 4 cut(s) 342, 790, 807, 865
NlaIII CATG 4 cut(s) 1109, 1244, 1319, 1441
NlaIV GGNNCC 2 cut(s) 809, 907
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 2 cut(s) 850, 1385
PaeR7I CTCGAG 1 cut(s) 669
PfeI GAWTC 3 cut(s) 770, 925, 1343
PflFI GACNNNGTC 2 cut(s) 409, 607
PflMI CCANNNNNTGG 2 cut(s) 1241, 1274
PleI GAGTC 6 cut(s) 418, 507, 513, 595, 1468, 1514
PmaCI CACGTG 1 cut(s) 1385
PmlI CACGTG 1 cut(s) 1385
PpsI GAGTC 6 cut(s) 418, 507, 513, 595, 1468, 1514
Ppu21I YACGTR 1 cut(s) 1385
PshBI ATTAAT 1 cut(s) 726
PsiI TTATAA 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 670
Psp6I CCWGG 2 cut(s) 810, 1337
PspCI CACGTG 1 cut(s) 1385
PspGI CCWGG 2 cut(s) 810, 1337
PspN4I GGNNCC 2 cut(s) 809, 907
PspPI GGNCC 1 cut(s) 814
PspXI VCTCGAGB 1 cut(s) 669
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 1 cut(s) 994
PsuI RGATCY 1 cut(s) 807
PsyI GACNNNGTC 2 cut(s) 409, 607
PvuII CAGCTG 2 cut(s) 979, 994
RsaI GTAC 5 cut(s) 694, 907, 968, 1028, 1211
RsaNI GTAC 5 cut(s) 693, 906, 967, 1027, 1210
SacI GAGCTC 1 cut(s) 670
SaqAI TTAA 3 cut(s) 726, 780, 1173
Sau3AI GATC 4 cut(s) 342, 790, 807, 865
Sau96I GGNCC 1 cut(s) 814
SchI GAGTC 6 cut(s) 419, 508, 514, 596, 1468, 1514
ScrFI CCNGG 2 cut(s) 812, 1339
SduI GDGCHC 1 cut(s) 670
SfaNI GCATC 4 cut(s) 23, 415, 441, 991
SfcI CTRYAG 2 cut(s) 582, 1098
Sfr274I CTCGAG 1 cut(s) 669
SinI GGWCC 1 cut(s) 814
SlaI CTCGAG 1 cut(s) 669
SmlI CTYRAG 1 cut(s) 669
SmoI CTYRAG 1 cut(s) 669
Sse9I AATT 5 cut(s) 328, 542, 796, 890, 1069
SsiI CCGC 8 cut(s) 23, 78, 126, 216, 247, 319, 333, 974
SspMI CTAG 1 cut(s) 648
SstI GAGCTC 1 cut(s) 670
StyD4I CCNGG 2 cut(s) 810, 1337
TaaI ACNGT 7 cut(s) 163, 826, 1085, 1324, 1358, 1419, 1496
TaiI ACGT 1 cut(s) 1387
TaqI TCGA 3 cut(s) 150, 363, 670
TasI AATT 5 cut(s) 328, 542, 796, 890, 1069
TatI WGTACW 1 cut(s) 1209
TauI GCSGC 3 cut(s) 81, 129, 321
TfiI GAWTC 3 cut(s) 770, 925, 1343
Tru1I TTAA 3 cut(s) 726, 780, 1173
Tru9I TTAA 3 cut(s) 726, 780, 1173
TscAI CASTG 3 cut(s) 386, 726, 855
TseFI GTSAC 2 cut(s) 850, 1385
Tsp45I GTSAC 2 cut(s) 850, 1385
TspDTI ATGAA 6 cut(s) 409, 618, 939, 1176, 1332, 1472
TspRI CASTG 3 cut(s) 386, 726, 855
Tth111I GACNNNGTC 2 cut(s) 409, 607
Van91I CCANNNNNTGG 2 cut(s) 1241, 1274
VpaK11BI GGWCC 1 cut(s) 814
VspI ATTAAT 1 cut(s) 726
XapI RAATTY 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 1 cut(s) 929
XhoI CTCGAG 1 cut(s) 669
XmiI GTMKAC 1 cut(s) 623
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.