Prupe.3G151600_v2.0.a1

RNA polymerase II transcription elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0021905
Physical Location & Seq
Forward (+)
16571829 .. 16575091
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G151600.1

Sequence Viewer

Length: 1038 bp
ATGGCTAAAAATCGGAAGAACAACGATAACGATGAACCAAACACTGCTCCAGAGCCTGACCAATGGTACAACCTATCCCTAGGTTCCTCCTTTGTGGATCACCGTCCCTCCCCAAAATTCTGTACTTTACGTTATGAATTTAAACCAGCTTCAATTGATAAGAATCAACCTGGTTCGTTCCACAAGAGCAAGGACAACAGGGTTACAGTTGAGTTTCACAACAACCAACAAGGAAAACCCAAGGTTACTTTTGAAGGTGTCAGTGAGGATTACAAGGAGCATGATGCTGTTTTGTTCTTTGATGGTGAGAGTTTTCGGTTGGAGAGGTTGCACCGAGCAGTGAAGCGGTTGAGACATCTAAGGCTGCCTGGTGAATCTGCAGCTCCAGCAGCCTCAGTCACGAGTACATCAATTTCAGCACTGGAATCTTATTCTCCACCATCTGGTGGAAAAGGAGCAAAGCTTCAGTCTTTGAACAAGGATGTTCTTCCTCATATGCCGGTGGAGGTGAAAGGAATTGATATCAACAACTCAGAGAGCATAGCCCCAGGCCCAACATCTGAAGCTGACAAGGATTTTGATTGTCCACCTTCCTTACCAAATCCACCAGCTGCATCTCCTGATGCAGAACCTAGAAGTGATGAGATGGATGAAGAAGTAGATGTTGTCATTGATGATGATGATGATGATGATGTTGTTGTGAATGAAACAACTGGGAAAGGAAAGTCTCCTGTAAATGAATTTCACACTGGCATTGACATTAACATACCTCATCCAGGTGATCTGGATGATGAGATTGCTGATGTAGATGTAGATGATGTGGTGGATAATGTCCCAAATGCTGCAGAAGCCCTTAGAGCTCAAGTGAATGCTGAGGTGGGAGGGAAGCAGACTTCAAGCTCTAGTGATAGTAGTGGGAGTGAGAGTAGTGGGAGTGAAAGCGGCAGTGGAAGTGGCAGTGGAAGTGAAAGCGAGAGTGCGAGTAGCAGCAGTGATACTGAAAGCAGTGAAGGTGGTGACTCAGTAAATTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

346

Amino Acids

36.8

Weight (kDa)

4.42

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021905)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38050
malus_domestica MD09G1260000.v1.1
prunus_persica Prupe.3G151600_v2.0.a1
pyrus_communis pycom09g17460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 443, 446
AciI CCGC 2 cut(s) 346, 942
AclWI GGATC 1 cut(s) 105
AcsI RAATTY 4 cut(s) 116, 137, 740, 1027
AcuI CTGAAG 2 cut(s) 449, 582
AfaI GTAC 3 cut(s) 68, 124, 406
AfiI CCNNNNNNNGG 3 cut(s) 443, 446, 776
AgsI TTSAA 4 cut(s) 153, 254, 475, 897
AjnI CCWGG 4 cut(s) 169, 367, 547, 775
AluBI AGCT 7 cut(s) 149, 383, 463, 566, 611, 860, 900
AluI AGCT 7 cut(s) 149, 383, 463, 566, 611, 860, 900
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 2 cut(s) 346, 732
AlwI GGATC 1 cut(s) 105
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 1 cut(s) 550
ApeKI GCWGC 6 cut(s) 364, 380, 389, 611, 842, 987
ApoI RAATTY 4 cut(s) 116, 137, 740, 1027
ArsI GACNNNNNNTTYG 4 cut(s) 452, 484, 560, 592
AspA2I CCTAGG 1 cut(s) 79
AspS9I GGNCC 1 cut(s) 551
AsuHPI GGTGA 6 cut(s) 92, 317, 383, 520, 791, 1028
AvrII CCTAGG 1 cut(s) 79
BanII GRGCYC 1 cut(s) 862
BauI CACGAG 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 862
BbvCI CCTCAGC 1 cut(s) 873
BbvI GCAGC 6 cut(s) 351, 392, 401, 598, 829, 999
BccI CCATC 3 cut(s) 296, 448, 640
BciT130I CCWGG 4 cut(s) 171, 369, 549, 777
BcoDI GTCTC 2 cut(s) 346, 732
BfaI CTAG 4 cut(s) 80, 633, 903, 1036
BfmI CTRYAG 2 cut(s) 378, 843
BisI GCNGC 7 cut(s) 365, 381, 390, 612, 843, 943, 988
BlnI CCTAGG 1 cut(s) 79
BlsI GCNGC 7 cut(s) 366, 382, 391, 613, 844, 944, 989
Bme1390I CCNGG 4 cut(s) 171, 369, 549, 777
BmgT120I GGNCC 1 cut(s) 551
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 4 cut(s) 171, 369, 549, 777
BmrI ACTGGG 1 cut(s) 723
BmsI GCATC 3 cut(s) 274, 613, 623
BmuI ACTGGG 1 cut(s) 723
BpmI CTGGAG 2 cut(s) 33, 369
Bpu10I CCTNAGC 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 846
BsaBI GATNNNNATC 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 79, 240, 547
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 910, 940
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 446, 776
Bse118I RCCGGY 1 cut(s) 499
Bse1I ACTGG 3 cut(s) 426, 718, 754
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 4 cut(s) 171, 369, 549, 777
BseDI CCNNGG 3 cut(s) 79, 240, 547
BseGI GGATG 4 cut(s) 487, 655, 772, 793
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 3 cut(s) 443, 446, 776
BseMII CTCAG 4 cut(s) 408, 546, 864, 1035
BseNI ACTGG 3 cut(s) 426, 718, 754
BseXI GCAGC 6 cut(s) 351, 392, 401, 598, 829, 999
BshFI GGCC 1 cut(s) 552
BsiHKAI GWGCWC 1 cut(s) 862
BsiSI CCGG 1 cut(s) 500
BslFI GGGAC 2 cut(s) 90, 818
BslI CCNNNNNNNGG 3 cut(s) 443, 446, 776
BsmAI GTCTC 2 cut(s) 346, 732
BsmFI GGGAC 2 cut(s) 90, 818
BsmI GAATGC 1 cut(s) 874
BsnI GGCC 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 862
Bsp143I GATC 2 cut(s) 97, 781
BspACI CCGC 2 cut(s) 346, 942
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 4 cut(s) 407, 545, 865, 1034
BspLI GGNNCC 1 cut(s) 85
BspMAI CTGCAG 2 cut(s) 382, 847
BspPI GGATC 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 499
BsrI ACTGG 3 cut(s) 426, 718, 754
BssAI RCCGGY 1 cut(s) 499
BssECI CCNNGG 3 cut(s) 79, 240, 547
BssMI GATC 2 cut(s) 97, 781
BssSI CACGAG 1 cut(s) 400
BssT1I CCWWGG 2 cut(s) 79, 240
Bst2BI CACGAG 1 cut(s) 400
Bst2UI CCWGG 4 cut(s) 171, 369, 549, 777
Bst4CI ACNGT 2 cut(s) 104, 208
BstDEI CTNAG 6 cut(s) 359, 394, 532, 854, 873, 1021
BstENI CCTNNNNNAGG 1 cut(s) 774
BstF5I GGATG 4 cut(s) 487, 655, 772, 793
BstKTI GATC 2 cut(s) 100, 784
BstMAI GTCTC 2 cut(s) 346, 732
BstMBI GATC 2 cut(s) 97, 781
BstMWI GCNNNNNNNGC 4 cut(s) 386, 389, 848, 857
BstNI CCWGG 4 cut(s) 171, 369, 549, 777
BstSCI CCNGG 4 cut(s) 169, 367, 547, 775
BstSFI CTRYAG 2 cut(s) 378, 843
BstV1I GCAGC 6 cut(s) 351, 392, 401, 598, 829, 999
BsuRI GGCC 1 cut(s) 552
BtsCI GGATG 4 cut(s) 487, 655, 772, 793
BtsI GCAGTG 6 cut(s) 42, 345, 952, 964, 997, 1012
BtsIMutI CAGTG 9 cut(s) 42, 268, 345, 419, 747, 952, 964, 997, 1012
CaiI CAGNNNCTG 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 499
Cfr13I GGNCC 1 cut(s) 551
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 3 cut(s) 67, 123, 405
CviAII CATG 1 cut(s) 281
CviQI GTAC 3 cut(s) 67, 123, 405
DdeI CTNAG 6 cut(s) 359, 394, 532, 854, 873, 1021
DpnI GATC 2 cut(s) 99, 783
DpnII GATC 2 cut(s) 97, 781
DraI TTTAAA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 860
Eco130I CCWWGG 2 cut(s) 79, 240
Eco24I GRGCYC 1 cut(s) 862
Eco32I GATATC 1 cut(s) 523
Eco53kI GAGCTC 1 cut(s) 860
Eco57I CTGAAG 2 cut(s) 449, 582
EcoICRI GAGCTC 1 cut(s) 860
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoRII CCWGG 4 cut(s) 169, 367, 547, 775
EcoRV GATATC 1 cut(s) 523
EcoT14I CCWWGG 2 cut(s) 79, 240
EcoT38I GRGCYC 1 cut(s) 862
ErhI CCWWGG 2 cut(s) 79, 240
FaeI CATG 1 cut(s) 284
FaiI YATR 6 cut(s) 135, 282, 495, 497, 542, 767
FaqI GGGAC 2 cut(s) 90, 818
FatI CATG 1 cut(s) 280
FauNDI CATATG 1 cut(s) 495
Fnu4HI GCNGC 7 cut(s) 365, 381, 390, 612, 843, 943, 988
FokI GGATG 4 cut(s) 494, 662, 759, 800
FriOI GRGCYC 1 cut(s) 862
Fsp4HI GCNGC 7 cut(s) 365, 381, 390, 612, 843, 943, 988
FspBI CTAG 4 cut(s) 80, 633, 903, 1036
GluI GCNGC 7 cut(s) 365, 381, 390, 612, 843, 943, 988
GsuI CTGGAG 2 cut(s) 33, 369
HaeIII GGCC 1 cut(s) 552
HapII CCGG 1 cut(s) 500
Hin1II CATG 1 cut(s) 284
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 4 cut(s) 163, 374, 425, 1019
HpaII CCGG 1 cut(s) 500
HphI GGTGA 6 cut(s) 92, 317, 383, 520, 791, 1028
Hpy166II GTNNAC 1 cut(s) 587
Hpy188I TCNGA 3 cut(s) 15, 535, 562
Hpy188III TCNNGA 4 cut(s) 50, 400, 620, 785
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 3 cut(s) 248, 600, 1004
HpyCH4III ACNGT 2 cut(s) 104, 208
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 5 cut(s) 331, 380, 614, 626, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 386, 389, 848, 857
HpyF3I CTNAG 6 cut(s) 359, 394, 532, 854, 873, 1021
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 1 cut(s) 284
Kzo9I GATC 2 cut(s) 97, 781
LmnI GCTCC 4 cut(s) 52, 277, 388, 455
Lsp1109I GCAGC 6 cut(s) 351, 392, 401, 598, 829, 999
LweI GCATC 3 cut(s) 274, 613, 623
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 4 cut(s) 80, 633, 903, 1036
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 4 cut(s) 202, 244, 397, 1016
MalI GATC 2 cut(s) 99, 783
MboI GATC 2 cut(s) 97, 781
MboII GAAGA 3 cut(s) 28, 479, 665
MfeI CAATTG 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 862
MluCI AATT 7 cut(s) 116, 137, 153, 411, 516, 740, 1027
MlyI GAGTC 1 cut(s) 1013
MmeI TCCRAC 1 cut(s) 300
MseI TTAA 2 cut(s) 141, 762
MslI CAYNNNNRTG 1 cut(s) 777
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 4 cut(s) 171, 369, 549, 777
MunI CAATTG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 874
MvaI CCWGG 4 cut(s) 171, 369, 549, 777
MwoI GCNNNNNNNGC 4 cut(s) 386, 389, 848, 857
NdeI CATATG 1 cut(s) 495
NdeII GATC 2 cut(s) 97, 781
NlaIII CATG 1 cut(s) 284
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 397, 1016
PctI GAATGC 1 cut(s) 874
PfeI GAWTC 3 cut(s) 163, 374, 425
PflMI CCANNNNNTGG 2 cut(s) 443, 446
PkrI GCNGC 7 cut(s) 366, 382, 391, 613, 844, 944, 989
PleI GAGTC 1 cut(s) 1013
PpsI GAGTC 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 862
Psp6I CCWGG 4 cut(s) 169, 367, 547, 775
PspGI CCWGG 4 cut(s) 169, 367, 547, 775
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 551
PstI CTGCAG 2 cut(s) 382, 847
PstNI CAGNNNCTG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 3 cut(s) 68, 124, 406
RsaNI GTAC 3 cut(s) 67, 123, 405
RseI CAYNNNNRTG 1 cut(s) 777
SacI GAGCTC 1 cut(s) 862
SaqAI TTAA 2 cut(s) 141, 762
SatI GCNGC 7 cut(s) 365, 381, 390, 612, 843, 943, 988
Sau3AI GATC 2 cut(s) 97, 781
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 1 cut(s) 1013
ScrFI CCNGG 4 cut(s) 171, 369, 549, 777
SduI GDGCHC 1 cut(s) 862
SexAI ACCWGGT 1 cut(s) 169
SfaNI GCATC 3 cut(s) 274, 613, 623
SfcI CTRYAG 2 cut(s) 378, 843
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 7 cut(s) 116, 137, 153, 411, 516, 740, 1027
SsiI CCGC 2 cut(s) 346, 942
SspMI CTAG 4 cut(s) 80, 633, 903, 1036
SstI GAGCTC 1 cut(s) 862
StyD4I CCNGG 4 cut(s) 169, 367, 547, 775
StyI CCWWGG 2 cut(s) 79, 240
TaaI ACNGT 2 cut(s) 104, 208
TaiI ACGT 1 cut(s) 133
TasI AATT 7 cut(s) 116, 137, 153, 411, 516, 740, 1027
TatI WGTACW 2 cut(s) 122, 404
TauI GCSGC 1 cut(s) 945
TfiI GAWTC 3 cut(s) 163, 374, 425
Tru1I TTAA 2 cut(s) 141, 762
Tru9I TTAA 2 cut(s) 141, 762
TscAI CASTG 9 cut(s) 49, 268, 345, 426, 754, 952, 964, 997, 1012
TseFI GTSAC 2 cut(s) 397, 1016
TseI GCWGC 6 cut(s) 364, 380, 389, 611, 842, 987
Tsp45I GTSAC 2 cut(s) 397, 1016
TspDTI ATGAA 5 cut(s) 48, 150, 666, 720, 753
TspRI CASTG 9 cut(s) 49, 268, 345, 426, 754, 952, 964, 997, 1012
Van91I CCANNNNNTGG 2 cut(s) 443, 446
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 4 cut(s) 116, 137, 740, 1027
XmaJI CCTAGG 1 cut(s) 79
XspI CTAG 4 cut(s) 80, 633, 903, 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.