Prupe.3G277400_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0029131
Physical Location & Seq
Reverse (-)
25491758 .. 25492630
873 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G277400.1

Sequence Viewer

Length: 873 bp
ATGCAACTTCATCAGAAACCCACAACAACAAAAATTACCTCGTCGCCATTCGAGTTCCTTGAGCATCTTAAGGGGTGCCACAAGGGTGACAAGGTCCAAGGCATCAAAGACCTGAAAAAATACCTCGGAAAATTTGGTTACTTGAGCAGAAACAATAATGGCCACTTCAATGATGATGATTTTGACGACCAATTGGAGTCTGCCATCAAAACTTACCAAATCAATTACCACCTCAAAGCCACCGGAACATTGGATGCCAAAACCGTATCCAACATGATGATGCCACGCTGTGGTGTTGCAGATATCATCAATGGTACCTCCTCCATGCGATCAGGCAAAAAAAGGCACCCTCACCATCACCACGGAGGACACACAGTTGCTCATTATTCTTTCTTCCAAGGAAACCCAAAATGGCCGGCCTCCAAGTATCACCTCACCTATGCTTTTCTTCAAGGCACCCCAGCTGAGGCCATGGGCCCAGTCTCCAGTGCTTTTCAAACATGGGCCGCCAATAAACACTTCACCTTCAGTGAGGTCCAGAGCAATCAGAACCCAGATCTGACTGTCAGTTTTCACAGGGGCAATCATGGAGATGGAGCCCCATTTGATGGGCCAGGTGGGACCATAGCCCATGCATTTGCTCCCACAAATGGGAGATTTCACTACGACGCTGCTGAGGCGTGGGCTGTGGGTGCCGTGACCGGTGCTTTTGACTTGCAGAGTGTAGCTCTGCATGAAATAGGGCACCTTCTCGGGCTGGGGCATAGCTCTGTTCAAGGAGCTATTATGTTCCCCTCTATCGGCACTGGAGTGACTCAGCAGAGCTTGCATGGGGATGATATTCAAGGAATTAAAGCTTTATACAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

291

Amino Acids

31.62

Weight (kDa)

7.91

Isoelectric Point (pI)

26.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029131)

Species Orthologous Gene IDs
prunus_persica Prupe.3G277400_v2.0.a1 Prupe.3G277700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 6 cut(s) 75, 314, 345, 455, 692, 744
AccB7I CCANNNNNTGG 2 cut(s) 290, 608
AciI CCGC 1 cut(s) 507
AcoI YGGCCR 2 cut(s) 160, 413
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 511
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 1 cut(s) 316
AfiI CCNNNNNNNGG 6 cut(s) 290, 466, 608, 650, 651, 800
AflII CTTAAG 1 cut(s) 68
AgeI ACCGGT 1 cut(s) 701
AgsI TTSAA 5 cut(s) 169, 452, 497, 776, 845
AjnI CCWGG 1 cut(s) 613
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AleI CACNNNNGTG 2 cut(s) 84, 809
AluBI AGCT 6 cut(s) 464, 728, 768, 782, 825, 857
AluI AGCT 6 cut(s) 464, 728, 768, 782, 825, 857
Alw26I GTCTC 1 cut(s) 487
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 7 cut(s) 160, 413, 417, 468, 475, 504, 611
ApaI GGGCCC 1 cut(s) 479
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 1 cut(s) 131
AsiGI ACCGGT 1 cut(s) 701
Asp718I GGTACC 1 cut(s) 314
AspS9I GGNCC 7 cut(s) 94, 475, 476, 504, 535, 611, 621
AsuHPI GGTGA 6 cut(s) 98, 344, 350, 422, 427, 514
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 3 cut(s) 94, 535, 621
BaeGI GKGCMC 2 cut(s) 479, 747
BalI TGGCCA 1 cut(s) 162
BanI GGYRCC 6 cut(s) 75, 314, 345, 455, 692, 744
BanII GRGCYC 2 cut(s) 479, 601
BbvCI CCTCAGC 2 cut(s) 465, 675
BbvI GCAGC 1 cut(s) 658
BccI CCATC 4 cut(s) 212, 363, 587, 602
BceAI ACGGC 1 cut(s) 680
BciT130I CCWGG 1 cut(s) 615
BciVI GTATCC 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 487
BfrI CTTAAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 277
BglII AGATCT 1 cut(s) 556
BisI GCNGC 2 cut(s) 507, 672
BlsI GCNGC 2 cut(s) 508, 673
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 3 cut(s) 94, 535, 621
BmeT110I CYCGRG 1 cut(s) 752
BmgT120I GGNCC 7 cut(s) 94, 475, 476, 504, 535, 611, 621
BmiI GGNNCC 9 cut(s) 77, 316, 347, 457, 477, 598, 622, 694, 746
BmrFI CCNGG 1 cut(s) 615
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 4 cut(s) 73, 111, 244, 270
BmuI ACTGGG 1 cut(s) 473
BplI GAGNNNNNCTC 2 cut(s) 712, 744
BpmI CTGGAG 2 cut(s) 469, 828
Bpu10I CCTNAGC 2 cut(s) 465, 675
BpuEI CTTGAG 2 cut(s) 80, 163
BsaJI CCNNGG 5 cut(s) 97, 124, 361, 397, 471
BsaWI WCCGGW 2 cut(s) 242, 701
Bsc4I CCNNNNNNNGG 6 cut(s) 290, 466, 608, 650, 651, 800
Bse118I RCCGGY 2 cut(s) 415, 701
Bse1I ACTGG 3 cut(s) 479, 486, 811
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 5 cut(s) 97, 124, 361, 397, 471
BseGI GGATG 2 cut(s) 259, 841
BseLI CCNNNNNNNGG 6 cut(s) 290, 466, 608, 650, 651, 800
BseMII CTCAG 3 cut(s) 456, 666, 830
BseNI ACTGG 3 cut(s) 479, 486, 811
BseRI GAGGAG 1 cut(s) 310
BseSI GKGCMC 2 cut(s) 479, 747
BseXI GCAGC 1 cut(s) 658
BseYI CCCAGC 2 cut(s) 460, 757
BshFI GGCC 7 cut(s) 162, 415, 419, 470, 477, 506, 613
BshNI GGYRCC 6 cut(s) 75, 314, 345, 455, 692, 744
BshTI ACCGGT 1 cut(s) 701
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 3 cut(s) 243, 416, 702
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 6 cut(s) 290, 466, 608, 650, 651, 800
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 634
BsnI GGCC 7 cut(s) 162, 415, 419, 470, 477, 506, 613
BsoBI CYCGRG 1 cut(s) 752
Bsp120I GGGCCC 1 cut(s) 475
Bsp1286I GDGCHC 3 cut(s) 479, 601, 747
Bsp143I GATC 2 cut(s) 329, 556
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 1 cut(s) 507
BspANI GGCC 7 cut(s) 162, 415, 419, 470, 477, 506, 613
BspCNI CTCAG 3 cut(s) 457, 667, 829
BspLI GGNNCC 9 cut(s) 77, 316, 347, 457, 477, 598, 622, 694, 746
BspT107I GGYRCC 6 cut(s) 75, 314, 345, 455, 692, 744
BspTI CTTAAG 1 cut(s) 68
BsrFI RCCGGY 2 cut(s) 415, 701
BsrI ACTGG 3 cut(s) 479, 486, 811
BssAI RCCGGY 2 cut(s) 415, 701
BssECI CCNNGG 5 cut(s) 97, 124, 361, 397, 471
BssMI GATC 2 cut(s) 329, 556
BssT1I CCWWGG 3 cut(s) 97, 397, 471
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 3 cut(s) 265, 376, 565
BstAFI CTTAAG 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 826
BstC8I GCNNGC 2 cut(s) 417, 827
BstDEI CTNAG 3 cut(s) 465, 675, 816
BstDSI CCRYGG 2 cut(s) 361, 471
BstF5I GGATG 2 cut(s) 259, 841
BstKTI GATC 2 cut(s) 332, 559
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 2 cut(s) 329, 556
BstMWI GCNNNNNNNGC 3 cut(s) 677, 692, 826
BstNI CCWGG 1 cut(s) 615
BstSCI CCNGG 1 cut(s) 613
BstSLI GKGCMC 2 cut(s) 479, 747
BstV1I GCAGC 1 cut(s) 658
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 7 cut(s) 162, 415, 419, 470, 477, 506, 613
BtgI CCRYGG 2 cut(s) 361, 471
BtsCI GGATG 2 cut(s) 259, 841
BtsIMutI CAGTG 3 cut(s) 493, 535, 804
Cac8I GCNNGC 2 cut(s) 417, 827
Cfr10I RCCGGY 2 cut(s) 415, 701
Cfr13I GGNCC 7 cut(s) 94, 475, 476, 504, 535, 611, 621
CseI GACGC 1 cut(s) 677
Csp6I GTAC 1 cut(s) 315
CspAI ACCGGT 1 cut(s) 701
CviAII CATG 8 cut(s) 274, 325, 472, 501, 587, 632, 734, 830
CviQI GTAC 1 cut(s) 315
DdeI CTNAG 3 cut(s) 465, 675, 816
DpnI GATC 2 cut(s) 331, 558
DpnII GATC 2 cut(s) 329, 556
DraIII CACNNNGTG 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 160, 413
Eco130I CCWWGG 3 cut(s) 97, 397, 471
Eco24I GRGCYC 2 cut(s) 479, 601
Eco32I GATATC 1 cut(s) 304
Eco47I GGWCC 3 cut(s) 94, 535, 621
Eco57I CTGAAG 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 752
EcoRII CCWGG 1 cut(s) 613
EcoRV GATATC 1 cut(s) 304
EcoT14I CCWWGG 3 cut(s) 97, 397, 471
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 2 cut(s) 479, 601
ErhI CCWWGG 3 cut(s) 97, 397, 471
FaeI CATG 8 cut(s) 277, 328, 475, 504, 590, 635, 737, 833
FaqI GGGAC 1 cut(s) 634
FatI CATG 8 cut(s) 273, 324, 471, 500, 586, 631, 733, 829
Fnu4HI GCNGC 2 cut(s) 507, 672
FokI GGATG 2 cut(s) 266, 848
FriOI GRGCYC 2 cut(s) 479, 601
FseI GGCCGGCC 1 cut(s) 419
Fsp4HI GCNGC 2 cut(s) 507, 672
GluI GCNGC 2 cut(s) 507, 672
GsaI CCCAGC 2 cut(s) 464, 761
GsuI CTGGAG 2 cut(s) 469, 828
HaeIII GGCC 7 cut(s) 162, 415, 419, 470, 477, 506, 613
HapII CCGG 3 cut(s) 243, 416, 702
HgaI GACGC 1 cut(s) 677
Hin1II CATG 8 cut(s) 277, 328, 475, 504, 590, 635, 737, 833
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 2 cut(s) 197, 814
HpaII CCGG 3 cut(s) 243, 416, 702
HphI GGTGA 6 cut(s) 98, 344, 350, 422, 427, 514
Hpy188I TCNGA 4 cut(s) 15, 128, 549, 561
Hpy188III TCNNGA 1 cut(s) 538
Hpy99I CGWCG 2 cut(s) 46, 671
HpyAV CCTTC 2 cut(s) 535, 758
HpyCH4III ACNGT 3 cut(s) 265, 376, 565
HpyCH4V TGCA 6 cut(s) 4, 299, 635, 718, 733, 829
HpyF10VI GCNNNNNNNGC 3 cut(s) 677, 692, 826
HpyF3I CTNAG 3 cut(s) 465, 675, 816
Hsp92II CATG 8 cut(s) 277, 328, 475, 504, 590, 635, 737, 833
KpnI GGTACC 1 cut(s) 318
KroI GCCGGC 1 cut(s) 415
KroNI GCCGGC 1 cut(s) 417
Kzo9I GATC 2 cut(s) 329, 556
LmnI GCTCC 3 cut(s) 596, 646, 779
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 4 cut(s) 73, 111, 244, 270
MaeIII GTNAC 4 cut(s) 86, 137, 697, 811
MalI GATC 2 cut(s) 331, 558
MboI GATC 2 cut(s) 329, 556
MboII GAAGA 2 cut(s) 385, 440
MfeI CAATTG 1 cut(s) 191
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 3 cut(s) 479, 601, 747
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 5 cut(s) 33, 131, 191, 223, 849
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 2 cut(s) 206, 808
MmeI TCCRAC 1 cut(s) 294
Mox20I TGGCCA 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 637
MroNI GCCGGC 1 cut(s) 415
MscI TGGCCA 1 cut(s) 162
MseI TTAA 2 cut(s) 69, 852
MslI CAYNNNNRTG 6 cut(s) 84, 168, 278, 591, 809, 834
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 68
MspI CCGG 3 cut(s) 243, 416, 702
MspR9I CCNGG 1 cut(s) 615
MunI CAATTG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 3 cut(s) 677, 692, 826
NaeI GCCGGC 1 cut(s) 417
NcoI CCATGG 1 cut(s) 471
NdeII GATC 2 cut(s) 329, 556
NgoMIV GCCGGC 1 cut(s) 415
NlaIII CATG 8 cut(s) 277, 328, 475, 504, 590, 635, 737, 833
NlaIV GGNNCC 9 cut(s) 77, 316, 347, 457, 477, 598, 622, 694, 746
NmuCI GTSAC 3 cut(s) 86, 697, 811
NsiI ATGCAT 1 cut(s) 637
OliI CACNNNNGTG 2 cut(s) 84, 809
PdiI GCCGGC 1 cut(s) 417
PflFI GACNNNGTC 1 cut(s) 92
PflMI CCANNNNNTGG 2 cut(s) 290, 608
PinAI ACCGGT 1 cut(s) 701
PkrI GCNGC 2 cut(s) 508, 673
PleI GAGTC 2 cut(s) 205, 808
PpsI GAGTC 2 cut(s) 205, 808
Psp6I CCWGG 1 cut(s) 613
PspFI CCCAGC 2 cut(s) 460, 757
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 9 cut(s) 77, 316, 347, 457, 477, 598, 622, 694, 746
PspOMI GGGCCC 1 cut(s) 475
PspPI GGNCC 7 cut(s) 94, 475, 476, 504, 535, 611, 621
PsuI RGATCY 1 cut(s) 556
PsyI GACNNNGTC 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 464
RigI GGCCGGCC 1 cut(s) 419
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
RseI CAYNNNNRTG 6 cut(s) 84, 168, 278, 591, 809, 834
SaqAI TTAA 2 cut(s) 69, 852
SatI GCNGC 2 cut(s) 507, 672
Sau3AI GATC 2 cut(s) 329, 556
Sau96I GGNCC 7 cut(s) 94, 475, 476, 504, 535, 611, 621
SchI GAGTC 2 cut(s) 206, 808
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 3 cut(s) 479, 601, 747
SfaNI GCATC 4 cut(s) 73, 111, 244, 270
SinI GGWCC 3 cut(s) 94, 535, 621
SmiMI CAYNNNNRTG 6 cut(s) 84, 168, 278, 591, 809, 834
SmlI CTYRAG 3 cut(s) 59, 68, 142
SmoI CTYRAG 3 cut(s) 59, 68, 142
Sse9I AATT 5 cut(s) 33, 131, 191, 223, 849
SsiI CCGC 1 cut(s) 507
StyD4I CCNGG 1 cut(s) 613
StyI CCWWGG 3 cut(s) 97, 397, 471
TaaI ACNGT 3 cut(s) 265, 376, 565
TaqI TCGA 1 cut(s) 51
TasI AATT 5 cut(s) 33, 131, 191, 223, 849
TauI GCSGC 1 cut(s) 509
Tru1I TTAA 2 cut(s) 69, 852
Tru9I TTAA 2 cut(s) 69, 852
TscAI CASTG 3 cut(s) 493, 535, 811
TseFI GTSAC 3 cut(s) 86, 697, 811
TseI GCWGC 1 cut(s) 671
Tsp45I GTSAC 3 cut(s) 86, 697, 811
TspDTI ATGAA 1 cut(s) 750
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 3 cut(s) 493, 535, 811
Tth111I GACNNNGTC 1 cut(s) 92
Van91I CCANNNNNTGG 2 cut(s) 290, 608
Vha464I CTTAAG 1 cut(s) 68
VpaK11BI GGWCC 3 cut(s) 94, 535, 621
XapI RAATTY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.