Prupe.3G277700_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0029131
Physical Location & Seq
Reverse (-)
25507019 .. 25507821
803 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G277700.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 753 bp
CATCTCAAGGGATGCCACAAGGGTGACAAGGTTCAAGGCATCCAAGACCTCAAAAAATACCTCGGAAAATTTGGTTACTTAAGCAGCAACAATAATGGCCACTTCAATGATGATGATTTTGACGACCAATTGGAGTCAGCCATCAAAACTTACCAAATCAATTACCACCTCAAAGCCACTGGAGCATTGGATGCCAAAACCGTATCCAATATGATGATGCCACGTTGCGGTGTCGCAGATATCATCAATGGTACCTCCTCCATGCGATCAGGCAAACAAAGGCACCCTCACCACCATCATCACGGAGGACACACAGTTGCTCATTATACTTTCTTCCGGGGAAACCCAAAATGGCCGGCCTCTAAGTATCACCTCACCTATGCTTTTCTTCAAGACACCCCAGCTGAAGCCACGGGACCAAGCAATCAGAACCCCGATCTGACGGTGAGTTTTCACAGGGGTAATCATGGAGACGGAGCTCCCTTTGACGGGCCAGGAGGGACCATAGCCCATGCATTTGCGCCAACGAACGGGAGATTTCACTACGACGCTGATGAGCGGTTTTCTGTGGGCGCCGTGAGTGGTGCTTATGACTTGGAGACTGTGGCTTTGCATGAAATAGGGCACCTTCTTGGGCTTGGGCACAGCTCTGTTCAGGGAGCTATTATGTACCCGACTATAAGCCCTGGAGTGACCCAGCAGAGCTTGCATGGGGATGATATTCAAGGAATTAAAGCTTTATACAACGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

251

Amino Acids

27.17

Weight (kDa)

6.74

Isoelectric Point (pI)

25.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029131)

Species Orthologous Gene IDs
prunus_persica Prupe.3G277400_v2.0.a1 Prupe.3G277700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 251
AccB1I GGYRCC 4 cut(s) 251, 282, 572, 624
AccBSI CCGCTC 1 cut(s) 559
AciI CCGC 2 cut(s) 228, 559
AcoI YGGCCR 2 cut(s) 97, 353
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 2 cut(s) 253, 671
AfiI CCNNNNNNNGG 4 cut(s) 227, 488, 489, 530
AflII CTTAAG 1 cut(s) 79
AgsI TTSAA 4 cut(s) 35, 106, 392, 725
AjnI CCWGG 2 cut(s) 493, 685
AleI CACNNNNGTG 1 cut(s) 21
AluBI AGCT 6 cut(s) 404, 479, 648, 662, 705, 737
AluI AGCT 6 cut(s) 404, 479, 648, 662, 705, 737
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 2 cut(s) 465, 593
AoxI GGCC 4 cut(s) 97, 353, 357, 491
ApeKI GCWGC 1 cut(s) 84
ApoI RAATTY 1 cut(s) 68
ArsI GACNNNNNNTTYG 2 cut(s) 592, 624
Asp718I GGTACC 1 cut(s) 251
AspLEI GCGC 2 cut(s) 523, 575
AspS9I GGNCC 3 cut(s) 416, 491, 501
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 5 cut(s) 35, 281, 362, 367, 457
AvaII GGWCC 2 cut(s) 416, 501
BaeGI GKGCMC 2 cut(s) 627, 645
BalI TGGCCA 1 cut(s) 99
BanI GGYRCC 4 cut(s) 251, 282, 572, 624
BanII GRGCYC 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 481
BbvI GCAGC 1 cut(s) 96
BccI CCATC 2 cut(s) 149, 303
BceAI ACGGC 1 cut(s) 560
BciT130I CCWGG 2 cut(s) 495, 687
BciVI GTATCC 1 cut(s) 214
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 2 cut(s) 465, 593
BfoI RGCGCY 1 cut(s) 576
BfrI CTTAAG 1 cut(s) 79
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 1 cut(s) 85
BlsI GCNGC 1 cut(s) 86
Bme1390I CCNGG 3 cut(s) 338, 495, 687
Bme18I GGWCC 2 cut(s) 416, 501
BmgT120I GGNCC 3 cut(s) 416, 491, 501
BmiI GGNNCC 6 cut(s) 253, 284, 417, 502, 574, 626
BmrFI CCNGG 3 cut(s) 338, 495, 687
BmsI GCATC 4 cut(s) 2, 48, 181, 207
BpmI CTGGAG 2 cut(s) 201, 708
BpuMI CCSGG 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 4 cut(s) 61, 337, 411, 685
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 488, 489, 530
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 1 cut(s) 184
BseBI CCWGG 2 cut(s) 495, 687
BseDI CCNNGG 4 cut(s) 61, 337, 411, 685
BseGI GGATG 4 cut(s) 17, 39, 196, 721
BseLI CCNNNNNNNGG 4 cut(s) 227, 488, 489, 530
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 1 cut(s) 247
BseSI GKGCMC 2 cut(s) 627, 645
BseXI GCAGC 1 cut(s) 96
BseYI CCCAGC 2 cut(s) 400, 696
BshFI GGCC 4 cut(s) 99, 355, 359, 493
BshNI GGYRCC 4 cut(s) 251, 282, 572, 624
BsiHKAI GWGCWC 1 cut(s) 481
BsiSI CCGG 2 cut(s) 337, 356
BslFI GGGAC 2 cut(s) 429, 514
BslI CCNNNNNNNGG 4 cut(s) 227, 488, 489, 530
BsmAI GTCTC 2 cut(s) 465, 593
BsmBI CGTCTC 1 cut(s) 465
BsmFI GGGAC 2 cut(s) 429, 514
BsnI GGCC 4 cut(s) 99, 355, 359, 493
Bsp1286I GDGCHC 3 cut(s) 481, 627, 645
Bsp143I GATC 2 cut(s) 266, 436
BspACI CCGC 2 cut(s) 228, 559
BspANI GGCC 4 cut(s) 99, 355, 359, 493
BspLI GGNNCC 6 cut(s) 253, 284, 417, 502, 574, 626
BspT107I GGYRCC 4 cut(s) 251, 282, 572, 624
BspTI CTTAAG 1 cut(s) 79
BsrBI CCGCTC 1 cut(s) 559
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 4 cut(s) 61, 337, 411, 685
BssMI GATC 2 cut(s) 266, 436
BssNI GRCGYC 1 cut(s) 573
Bst2UI CCWGG 2 cut(s) 495, 687
Bst4CI ACNGT 4 cut(s) 202, 316, 445, 604
BstACI GRCGYC 1 cut(s) 573
BstAFI CTTAAG 1 cut(s) 79
BstAPI GCANNNNNTGC 2 cut(s) 191, 706
BstC8I GCNNGC 2 cut(s) 357, 707
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 4 cut(s) 17, 39, 196, 721
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 2 cut(s) 523, 575
BstKTI GATC 2 cut(s) 269, 439
BstMAI GTCTC 2 cut(s) 465, 593
BstMBI GATC 2 cut(s) 266, 436
BstMWI GCNNNNNNNGC 3 cut(s) 182, 191, 706
BstNI CCWGG 2 cut(s) 495, 687
BstSCI CCNGG 3 cut(s) 336, 493, 685
BstSLI GKGCMC 2 cut(s) 627, 645
BstV1I GCAGC 1 cut(s) 96
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 4 cut(s) 99, 355, 359, 493
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 4 cut(s) 17, 39, 196, 721
BtsIMutI CAGTG 1 cut(s) 177
Cac8I GCNNGC 2 cut(s) 357, 707
CfoI GCGC 2 cut(s) 523, 575
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 416, 491, 501
CseI GACGC 1 cut(s) 557
Csp6I GTAC 2 cut(s) 252, 670
CviAII CATG 5 cut(s) 262, 467, 512, 614, 710
CviQI GTAC 2 cut(s) 252, 670
DdeI CTNAG 1 cut(s) 363
DinI GGCGCC 1 cut(s) 574
DpnI GATC 2 cut(s) 268, 438
DpnII GATC 2 cut(s) 266, 436
EaeI YGGCCR 2 cut(s) 97, 353
Ecl136II GAGCTC 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 481
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 416, 501
Eco53kI GAGCTC 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 426
EcoICRI GAGCTC 1 cut(s) 479
EcoRII CCWGG 2 cut(s) 493, 685
EcoRV GATATC 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 481
EgeI GGCGCC 1 cut(s) 574
EheI GGCGCC 1 cut(s) 574
Esp3I CGTCTC 1 cut(s) 465
FaeI CATG 5 cut(s) 265, 470, 515, 617, 713
FaqI GGGAC 2 cut(s) 429, 514
FatI CATG 5 cut(s) 261, 466, 511, 613, 709
Fnu4HI GCNGC 1 cut(s) 85
FokI GGATG 4 cut(s) 24, 26, 203, 728
FriOI GRGCYC 1 cut(s) 481
FseI GGCCGGCC 1 cut(s) 359
Fsp4HI GCNGC 1 cut(s) 85
GlaI GCGC 2 cut(s) 522, 574
GluI GCNGC 1 cut(s) 85
GsaI CCCAGC 2 cut(s) 404, 700
GsuI CTGGAG 2 cut(s) 201, 708
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 4 cut(s) 99, 355, 359, 493
HapII CCGG 2 cut(s) 337, 356
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 523, 575
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 5 cut(s) 265, 470, 515, 617, 713
Hin6I GCGC 2 cut(s) 521, 573
HinP1I GCGC 2 cut(s) 521, 573
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 1 cut(s) 134
HpaII CCGG 2 cut(s) 337, 356
HphI GGTGA 5 cut(s) 35, 281, 362, 367, 457
Hpy188I TCNGA 3 cut(s) 65, 429, 441
Hpy188III TCNNGA 1 cut(s) 392
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 1 cut(s) 638
HpyCH4III ACNGT 4 cut(s) 202, 316, 445, 604
HpyCH4IV ACGT 1 cut(s) 223
HpyCH4V TGCA 3 cut(s) 515, 613, 709
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 191, 706
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 223
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 5 cut(s) 265, 470, 515, 617, 713
HspAI GCGC 2 cut(s) 521, 573
KasI GGCGCC 1 cut(s) 572
KpnI GGTACC 1 cut(s) 255
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Kzo9I GATC 2 cut(s) 266, 436
LmnI GCTCC 4 cut(s) 182, 476, 484, 659
Lsp1109I GCAGC 1 cut(s) 96
LweI GCATC 4 cut(s) 2, 48, 181, 207
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 3 cut(s) 23, 74, 691
MalI GATC 2 cut(s) 268, 438
MbiI CCGCTC 1 cut(s) 559
MboI GATC 2 cut(s) 266, 436
MboII GAAGA 2 cut(s) 325, 380
MfeI CAATTG 1 cut(s) 128
MhlI GDGCHC 3 cut(s) 481, 627, 645
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 4 cut(s) 68, 128, 160, 729
MluNI TGGCCA 1 cut(s) 99
Mly113I GGCGCC 1 cut(s) 573
MlyI GAGTC 1 cut(s) 143
Mox20I TGGCCA 1 cut(s) 99
Mph1103I ATGCAT 1 cut(s) 517
MroNI GCCGGC 1 cut(s) 355
MscI TGGCCA 1 cut(s) 99
MseI TTAA 2 cut(s) 80, 732
MslI CAYNNNNRTG 3 cut(s) 21, 105, 714
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 404
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 2 cut(s) 337, 356
MspR9I CCNGG 3 cut(s) 338, 495, 687
MunI CAATTG 1 cut(s) 128
MvaI CCWGG 2 cut(s) 495, 687
MwoI GCNNNNNNNGC 3 cut(s) 182, 191, 706
NaeI GCCGGC 1 cut(s) 357
NarI GGCGCC 1 cut(s) 573
NciI CCSGG 1 cut(s) 338
NdeII GATC 2 cut(s) 266, 436
NgoMIV GCCGGC 1 cut(s) 355
NlaIII CATG 5 cut(s) 265, 470, 515, 617, 713
NlaIV GGNNCC 6 cut(s) 253, 284, 417, 502, 574, 626
NmuCI GTSAC 2 cut(s) 23, 691
NsiI ATGCAT 1 cut(s) 517
OliI CACNNNNGTG 1 cut(s) 21
PdiI GCCGGC 1 cut(s) 357
PkrI GCNGC 1 cut(s) 86
PleI GAGTC 1 cut(s) 142
PluTI GGCGCC 1 cut(s) 576
PpsI GAGTC 1 cut(s) 142
Psp124BI GAGCTC 1 cut(s) 481
Psp6I CCWGG 2 cut(s) 493, 685
PspFI CCCAGC 2 cut(s) 400, 696
PspGI CCWGG 2 cut(s) 493, 685
PspN4I GGNNCC 6 cut(s) 253, 284, 417, 502, 574, 626
PspPI GGNCC 3 cut(s) 416, 491, 501
PvuII CAGCTG 1 cut(s) 404
RigI GGCCGGCC 1 cut(s) 359
RsaI GTAC 2 cut(s) 253, 671
RsaNI GTAC 2 cut(s) 252, 670
RseI CAYNNNNRTG 3 cut(s) 21, 105, 714
SacI GAGCTC 1 cut(s) 481
SaqAI TTAA 2 cut(s) 80, 732
SatI GCNGC 1 cut(s) 85
Sau3AI GATC 2 cut(s) 266, 436
Sau96I GGNCC 3 cut(s) 416, 491, 501
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 3 cut(s) 338, 495, 687
SduI GDGCHC 3 cut(s) 481, 627, 645
SfaNI GCATC 4 cut(s) 2, 48, 181, 207
SfoI GGCGCC 1 cut(s) 574
SinI GGWCC 2 cut(s) 416, 501
SmiMI CAYNNNNRTG 3 cut(s) 21, 105, 714
SmlI CTYRAG 2 cut(s) 5, 79
SmoI CTYRAG 2 cut(s) 5, 79
Sse9I AATT 4 cut(s) 68, 128, 160, 729
SsiI CCGC 2 cut(s) 228, 559
SspDI GGCGCC 1 cut(s) 572
SstI GAGCTC 1 cut(s) 481
StyD4I CCNGG 3 cut(s) 336, 493, 685
TaaI ACNGT 4 cut(s) 202, 316, 445, 604
TaiI ACGT 1 cut(s) 226
TasI AATT 4 cut(s) 68, 128, 160, 729
Tru1I TTAA 2 cut(s) 80, 732
Tru9I TTAA 2 cut(s) 80, 732
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 2 cut(s) 23, 691
TseI GCWGC 1 cut(s) 84
Tsp45I GTSAC 2 cut(s) 23, 691
TspDTI ATGAA 1 cut(s) 630
TspGWI ACGGA 2 cut(s) 318, 489
TspRI CASTG 1 cut(s) 184
Vha464I CTTAAG 1 cut(s) 79
VpaK11BI GGWCC 2 cut(s) 416, 501
XapI RAATTY 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.