Prupe.4G020200_v2.0.a1

cellular response to nitrogen starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0021231
Physical Location & Seq
Forward (+)
948085 .. 950398
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G020200.3_v2.0.a1.CDS.1

Sequence Viewer

Length: 594 bp
ATGGCCGAAAGCTCTTGCAAGCTTGAAAATCATCTGGAAACGATGCGAGCAGAAGAGAACATTTCAGATCCAGCCCCAATGGTTGTGAAGAATGCTGCCGCTGCGGCTTCCCTAAGTGTCATTCCTGACATTAATAAGATGAAGAAGAAGAAGAAGAGGAAACCGACAGTAGTAATATCTCCGGTTAGAGCTTTAATTTTAGACACTTGGAATATGAGGCACGTGTGGAAGGATGCGAATGGTATAAGTGGTACTGAAAAACATATAGACCCTTGGCTTTTGAGGGAGACGGACCCAGATTTGGTACCAGTGGCTGTGGAGAAGGATGCAAGGAGTGATATTGCTGAGATTGACGCGAAGAAGTACCTTCTCCCCAAGGATGCACTTCTTGGAGATTTTATTGCTTATGTTCGGATGTGGATCTTTCTAAAGAAAAGAAAACCTATATTTGTCTTCTTCAAAAACACTGTACCTCCCACAGGTGCCTCTATGGGTGCAGTTGATGAGGAAAATAAGGATGATGATGGATTTCTTCACATGACCTACAGTGGGAATGATACCTTCAGCTACAGTGGGAATGTTGTGACCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.07

Weight (kDa)

6.91

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 304
AccB1I GGYRCC 2 cut(s) 304, 482
AccII CGCG 1 cut(s) 356
AciI CCGC 2 cut(s) 99, 104
AclWI GGATC 2 cut(s) 62, 428
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 547
AcvI CACGTG 1 cut(s) 223
AfaI GTAC 4 cut(s) 253, 306, 365, 471
AfiI CCNNNNNNNGG 3 cut(s) 301, 479, 549
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 26, 460
AluBI AGCT 4 cut(s) 12, 22, 191, 567
AluI AGCT 4 cut(s) 12, 22, 191, 567
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 2 cut(s) 62, 428
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 95, 101
AseI ATTAAT 1 cut(s) 132
Asp718I GGTACC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 292
AvaII GGWCC 1 cut(s) 292
BanI GGYRCC 2 cut(s) 304, 482
BbrPI CACGTG 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 445
BbvI GCAGC 2 cut(s) 82, 88
BccI CCATC 1 cut(s) 518
BcoDI GTCTC 1 cut(s) 281
BfmI CTRYAG 2 cut(s) 544, 568
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 4 cut(s) 96, 99, 102, 105
BlsI GCNGC 4 cut(s) 97, 100, 103, 106
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 3 cut(s) 294, 306, 484
BmsI GCATC 4 cut(s) 33, 223, 316, 370
BpiI GAAGAC 1 cut(s) 445
BsaAI YACGTR 1 cut(s) 223
BsaBI GATNNNNATC 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 272, 375
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 479, 549
Bse1I ACTGG 1 cut(s) 308
Bse8I GATNNNNATC 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 272, 375
BseGI GGATG 5 cut(s) 238, 331, 385, 420, 523
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 3 cut(s) 301, 479, 549
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 1 cut(s) 308
BseXI GCAGC 2 cut(s) 82, 88
BsgI GTGCAG 1 cut(s) 516
Bsh1236I CGCG 1 cut(s) 356
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 304, 482
BsiSI CCGG 1 cut(s) 182
BslI CCNNNNNNNGG 3 cut(s) 301, 479, 549
BsmAI GTCTC 1 cut(s) 281
BsmBI CGTCTC 1 cut(s) 281
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 67, 420
BspACI CCGC 2 cut(s) 99, 104
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 337
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 3 cut(s) 294, 306, 484
BspPI GGATC 2 cut(s) 62, 428
BspT107I GGYRCC 2 cut(s) 304, 482
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 272, 375
BssMI GATC 2 cut(s) 67, 420
BssT1I CCWWGG 2 cut(s) 272, 375
Bst4CI ACNGT 4 cut(s) 169, 469, 548, 572
Bst6I CTCTTC 2 cut(s) 48, 149
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 20, 48
BstDEI CTNAG 2 cut(s) 113, 345
BstENI CCTNNNNNAGG 1 cut(s) 477
BstF5I GGATG 5 cut(s) 238, 331, 385, 420, 523
BstFNI CGCG 1 cut(s) 356
BstKTI GATC 2 cut(s) 70, 423
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 2 cut(s) 67, 420
BstMWI GCNNNNNNNGC 2 cut(s) 101, 104
BstSFI CTRYAG 2 cut(s) 544, 568
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 2 cut(s) 82, 88
BstV2I GAAGAC 1 cut(s) 445
BstX2I RGATCY 2 cut(s) 67, 420
BstYI RGATCY 2 cut(s) 67, 420
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 5 cut(s) 238, 331, 385, 420, 523
BtsIMutI CAGTG 4 cut(s) 315, 465, 553, 577
Cac8I GCNNGC 2 cut(s) 20, 48
CaiI CAGNNNCTG 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 292
CseI GACGC 1 cut(s) 362
Csp6I GTAC 4 cut(s) 252, 305, 364, 470
CviAII CATG 1 cut(s) 538
CviJI RGCY 9 cut(s) 5, 12, 22, 74, 107, 191, 277, 314, 567
CviKI_1 RGCY 9 cut(s) 5, 12, 22, 74, 107, 191, 277, 314, 567
CviQI GTAC 4 cut(s) 252, 305, 364, 470
DdeI CTNAG 2 cut(s) 113, 345
DpnI GATC 2 cut(s) 69, 422
DpnII GATC 2 cut(s) 67, 420
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 48, 149
EarI CTCTTC 2 cut(s) 48, 149
Eco130I CCWWGG 2 cut(s) 272, 375
Eco47I GGWCC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 547
Eco72I CACGTG 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 477
EcoT14I CCWWGG 2 cut(s) 272, 375
ErhI CCWWGG 2 cut(s) 272, 375
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 1 cut(s) 541
FaiI YATR 8 cut(s) 215, 245, 264, 266, 408, 446, 491, 539
FatI CATG 1 cut(s) 537
Fnu4HI GCNGC 4 cut(s) 96, 99, 102, 105
FokI GGATG 5 cut(s) 245, 338, 392, 427, 530
Fsp4HI GCNGC 4 cut(s) 96, 99, 102, 105
GluI GCNGC 4 cut(s) 96, 99, 102, 105
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 182
HgaI GACGC 1 cut(s) 362
Hin1II CATG 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 20
HpaII CCGG 1 cut(s) 182
Hpy188I TCNGA 2 cut(s) 67, 414
Hpy188III TCNNGA 2 cut(s) 35, 125
HpyAV CCTTC 4 cut(s) 223, 316, 377, 571
HpyCH4III ACNGT 4 cut(s) 169, 469, 548, 572
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 4 cut(s) 18, 329, 383, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 104
HpyF3I CTNAG 2 cut(s) 113, 345
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 1 cut(s) 541
KpnI GGTACC 1 cut(s) 308
Kzo9I GATC 2 cut(s) 67, 420
LpnPI CCDG 7 cut(s) 20, 84, 138, 195, 309, 321, 465
Lsp1109I GCAGC 2 cut(s) 82, 88
LweI GCATC 4 cut(s) 33, 223, 316, 370
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 583
MalI GATC 2 cut(s) 69, 422
MboI GATC 2 cut(s) 67, 420
MflI RGATCY 2 cut(s) 67, 420
MluCI AATT 1 cut(s) 195
MnlI CCTC 6 cut(s) 150, 210, 276, 483, 496, 499
MseI TTAA 2 cut(s) 132, 194
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 97
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 2 cut(s) 101, 104
NdeII GATC 2 cut(s) 67, 420
NlaIII CATG 1 cut(s) 541
NlaIV GGNNCC 3 cut(s) 294, 306, 484
NmuCI GTSAC 1 cut(s) 583
PctI GAATGC 1 cut(s) 97
PkrI GCNGC 4 cut(s) 97, 100, 103, 106
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 223
PshBI ATTAAT 1 cut(s) 132
PspCI CACGTG 1 cut(s) 223
PspN4I GGNNCC 3 cut(s) 294, 306, 484
PspPI GGNCC 1 cut(s) 292
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 2 cut(s) 67, 420
RsaI GTAC 4 cut(s) 253, 306, 365, 471
RsaNI GTAC 4 cut(s) 252, 305, 364, 470
SaqAI TTAA 2 cut(s) 132, 194
SatI GCNGC 4 cut(s) 96, 99, 102, 105
Sau3AI GATC 2 cut(s) 67, 420
Sau96I GGNCC 1 cut(s) 292
SfaNI GCATC 4 cut(s) 33, 223, 316, 370
SfcI CTRYAG 2 cut(s) 544, 568
SinI GGWCC 1 cut(s) 292
Sse9I AATT 1 cut(s) 195
SsiI CCGC 2 cut(s) 99, 104
StyI CCWWGG 2 cut(s) 272, 375
TaaI ACNGT 4 cut(s) 169, 469, 548, 572
TaiI ACGT 1 cut(s) 225
TasI AATT 1 cut(s) 195
TauI GCSGC 2 cut(s) 101, 107
Tru1I TTAA 2 cut(s) 132, 194
Tru9I TTAA 2 cut(s) 132, 194
TscAI CASTG 4 cut(s) 315, 472, 553, 577
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 2 cut(s) 95, 101
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 4 cut(s) 315, 472, 553, 577
VpaK11BI GGWCC 1 cut(s) 292
VspI ATTAAT 1 cut(s) 132
XagI CCTNNNNNAGG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.